Ecology of Trypanosoma cruzi I genotypes across Rhodnius prolixus captured in Attalea butyracea palms

Trypanosoma cruzi, the agent of Chagas disease exhibits significant genetic diversity. This parasite is divided into six discrete typing units (DTUs) where T. cruzi I (TcI) is the most widespread in the Americas. TcI genotypes have been associated to domestic and sylvatic cycles of transmission (TcI...

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Autores:
Tipo de recurso:
Fecha de publicación:
2017
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
eng
OAI Identifier:
oai:repository.urosario.edu.co:10336/24302
Acceso en línea:
https://doi.org/10.1016/j.meegid.2017.01.017
https://repository.urosario.edu.co/handle/10336/24302
Palabra clave:
Adult
Arecaceae
Article
Attalea butyracea
Controlled study
Disease control
Disease transmission
Molecular typing
Nonhuman
Principal component analysis
Rhodnius prolixus
Taxonomic identification
Trypanosoma cruzi
Trypanosoma cruzi i
Animal
Arecaceae
Chagas disease
Classification
Colombia
Ecosystem
Genetics
Genotype
Human
Insect control
Insect vector
Isolation and purification
Organization and management
Parasitology
Phylogeny
Plant leaf
Rhodnius
Transmission
Trypanosoma cruzi
Protozoal dna
Animals
Arecaceae
Chagas disease
Colombia
Ecosystem
Genotype
Humans
Insect control
Insect vectors
Molecular typing
Phylogeny
Plant leaves
Rhodnius
Trypanosoma cruzi
A
Butyracea
Chagas disease
Domestic cycle
Dtus
R
Prolixus
Sylvatic cycle
T
Cruzi
protozoan
Dna
Rights
License
Abierto (Texto Completo)
Description
Summary:Trypanosoma cruzi, the agent of Chagas disease exhibits significant genetic diversity. This parasite is divided into six discrete typing units (DTUs) where T. cruzi I (TcI) is the most widespread in the Americas. TcI genotypes have been associated to domestic and sylvatic cycles of transmission (TcIDom and sylvatic TcI). Due to the importance of the enzootic transmission, we determined the frequency of TcI genotypes present in Rhodnius prolixus captured in different regions of the palm A. butyracea to understand the ecology of the disease and the importance of A. butyracea palms as ecotopes of R. prolixus. Forty A. butyracea palms were sampled (base crown, mid-point and crown) capturing 105 individuals identified as R. prolixus by morphological and molecular barcoding. We conducted molecular detection and typing of T. cruzi across 59 individuals. The results showed that all the insects were infected with TcI; 28.57% were sylvatic TcI, 12.38% TcIDom and 15,24% mixed infections (TcIDom/sylvatic TcI). Statistical analysis showed a similar behavior between TcIDom and mixed infections in the mid-point and at the crown of the palm, being more frequent in the crown, while sylvatic TcI does not seem to have a specific association with any of the sampled areas. These findings are consistent with other studies showing high mobility of the insect vector between different ecotopes, increasing the need to develop improvements in the programs of disease control. © 2017 Elsevier B.V.