The role of potassium channels in the vasodilatory effect of caffeine in human internal mammary arteries

There is little information about the direct effect of caffeine in human blood vessels. The purpose of this study was to evaluate the direct vascular effect of caffeine on human internal mammary artery (IMA) and the involvement of potassium channels in this response. Segments of IMA were obtained fr...

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Tipo de recurso:
Fecha de publicación:
2009
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
eng
OAI Identifier:
oai:repository.urosario.edu.co:10336/23470
Acceso en línea:
https://doi.org/10.1016/j.vph.2008.11.002
https://repository.urosario.edu.co/handle/10336/23470
Palabra clave:
4 aminopyridine
Caffeine
Glibenclamide
Noradrenalin
Potassium channel
Potassium channel blocking agent
Tetrylammonium
Article
Bath
Clinical article
Concentration (parameters)
Coronary artery bypass surgery
Endothelium
Human
Human tissue
Incubation time
Internal mammary artery
Mental concentration
Priority journal
Vasodilatation
Caffeine
Glyburide
Humans
Mammary arteries
Potassium channel blockers
Potassium channels
Vasodilation
Caffeine
Internal mammary artery
Potassium channel
Vasodilation
drug
Dose-response relationship
Rights
License
Abierto (Texto Completo)
id EDOCUR2_1515edf0de9c7963cfa2426efea92c0b
oai_identifier_str oai:repository.urosario.edu.co:10336/23470
network_acronym_str EDOCUR2
network_name_str Repositorio EdocUR - U. Rosario
repository_id_str
spelling 5ee38339-ca7a-4ab8-8451-99d76f728a7a-1bc54061b-7ea1-45b8-b02f-a9c8e72476b1-1e6341c24-1ff3-4b44-9865-924cc5e9e94d-1f915930c-6cd7-4e6e-b876-868dddf68360-12d345211-adbe-4771-b6ac-a07098a3157c-12020-05-26T00:02:18Z2020-05-26T00:02:18Z2009There is little information about the direct effect of caffeine in human blood vessels. The purpose of this study was to evaluate the direct vascular effect of caffeine on human internal mammary artery (IMA) and the involvement of potassium channels in this response. Segments of IMA were obtained from 29 patients who underwent coronary artery bypass graft surgery. They were cut into rings, suspended between two wire hooks in organ bath chambers and constricted submaximally with norepinephrine. Caffeine (3.16 × 10- 9 to 10- 4 mol/L) was added in a cumulative fashion to rings with or without functional endothelium and concentration response curves were constructed. The response to caffeine was also evaluated after incubation with adenosine 3?,5?-triphosphate (ATP)-dependent potassium channel blocker glibenclamide, voltage-dependent potassium channel blocker 4-aminopyridine and large-conductance calcium-activated potassium channel inhibitor tetraethylammonium. Caffeine produced a potent, concentration-dependent relaxation of IMA. The relaxant responses did not differ significantly between endothelium-intact and endothelium-denuded preparations. Incubation with different potassium channel inhibitors (glibenclamide, 4-aminopyridine and tetraethylammonium) did not cause significant alterations in the relaxant responses to caffeine. These results suggest that the vasodilatory response to caffeine in human IMA is independent of endothelial function and is not mediated by potassium channels. © 2008 Elsevier Inc. All rights reserved.application/pdfhttps://doi.org/10.1016/j.vph.2008.11.00215371891https://repository.urosario.edu.co/handle/10336/23470eng136No. 43924132Vascular PharmacologyVol. 50Vascular Pharmacology, ISSN:15371891, Vol.50, No.43924 (2009); pp. 132-136https://www.scopus.com/inward/record.uri?eid=2-s2.0-58549102308&doi=10.1016%2fj.vph.2008.11.002&partnerID=40&md5=64ba0d0bea4da031b615f07b7da23ff6Abierto (Texto Completo)http://purl.org/coar/access_right/c_abf2instname:Universidad del Rosarioreponame:Repositorio Institucional EdocUR4 aminopyridineCaffeineGlibenclamideNoradrenalinPotassium channelPotassium channel blocking agentTetrylammoniumArticleBathClinical articleConcentration (parameters)Coronary artery bypass surgeryEndotheliumHumanHuman tissueIncubation timeInternal mammary arteryMental concentrationPriority journalVasodilatationCaffeineGlyburideHumansMammary arteriesPotassium channel blockersPotassium channelsVasodilationCaffeineInternal mammary arteryPotassium channelVasodilationdrugDose-response relationshipThe role of potassium channels in the vasodilatory effect of caffeine in human internal mammary arteriesarticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Montes, Felix R.Cabrera, MarianaDelgadillo, AlexandraSalgar, CarlosEcheverri, Dario10336/23470oai:repository.urosario.edu.co:10336/234702022-05-02 07:37:20.994807https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co
dc.title.spa.fl_str_mv The role of potassium channels in the vasodilatory effect of caffeine in human internal mammary arteries
title The role of potassium channels in the vasodilatory effect of caffeine in human internal mammary arteries
spellingShingle The role of potassium channels in the vasodilatory effect of caffeine in human internal mammary arteries
4 aminopyridine
Caffeine
Glibenclamide
Noradrenalin
Potassium channel
Potassium channel blocking agent
Tetrylammonium
Article
Bath
Clinical article
Concentration (parameters)
Coronary artery bypass surgery
Endothelium
Human
Human tissue
Incubation time
Internal mammary artery
Mental concentration
Priority journal
Vasodilatation
Caffeine
Glyburide
Humans
Mammary arteries
Potassium channel blockers
Potassium channels
Vasodilation
Caffeine
Internal mammary artery
Potassium channel
Vasodilation
drug
Dose-response relationship
title_short The role of potassium channels in the vasodilatory effect of caffeine in human internal mammary arteries
title_full The role of potassium channels in the vasodilatory effect of caffeine in human internal mammary arteries
title_fullStr The role of potassium channels in the vasodilatory effect of caffeine in human internal mammary arteries
title_full_unstemmed The role of potassium channels in the vasodilatory effect of caffeine in human internal mammary arteries
title_sort The role of potassium channels in the vasodilatory effect of caffeine in human internal mammary arteries
dc.subject.keyword.spa.fl_str_mv 4 aminopyridine
Caffeine
Glibenclamide
Noradrenalin
Potassium channel
Potassium channel blocking agent
Tetrylammonium
Article
Bath
Clinical article
Concentration (parameters)
Coronary artery bypass surgery
Endothelium
Human
Human tissue
Incubation time
Internal mammary artery
Mental concentration
Priority journal
Vasodilatation
Caffeine
Glyburide
Humans
Mammary arteries
Potassium channel blockers
Potassium channels
Vasodilation
Caffeine
Internal mammary artery
Potassium channel
Vasodilation
topic 4 aminopyridine
Caffeine
Glibenclamide
Noradrenalin
Potassium channel
Potassium channel blocking agent
Tetrylammonium
Article
Bath
Clinical article
Concentration (parameters)
Coronary artery bypass surgery
Endothelium
Human
Human tissue
Incubation time
Internal mammary artery
Mental concentration
Priority journal
Vasodilatation
Caffeine
Glyburide
Humans
Mammary arteries
Potassium channel blockers
Potassium channels
Vasodilation
Caffeine
Internal mammary artery
Potassium channel
Vasodilation
drug
Dose-response relationship
dc.subject.keyword.eng.fl_str_mv drug
Dose-response relationship
description There is little information about the direct effect of caffeine in human blood vessels. The purpose of this study was to evaluate the direct vascular effect of caffeine on human internal mammary artery (IMA) and the involvement of potassium channels in this response. Segments of IMA were obtained from 29 patients who underwent coronary artery bypass graft surgery. They were cut into rings, suspended between two wire hooks in organ bath chambers and constricted submaximally with norepinephrine. Caffeine (3.16 × 10- 9 to 10- 4 mol/L) was added in a cumulative fashion to rings with or without functional endothelium and concentration response curves were constructed. The response to caffeine was also evaluated after incubation with adenosine 3?,5?-triphosphate (ATP)-dependent potassium channel blocker glibenclamide, voltage-dependent potassium channel blocker 4-aminopyridine and large-conductance calcium-activated potassium channel inhibitor tetraethylammonium. Caffeine produced a potent, concentration-dependent relaxation of IMA. The relaxant responses did not differ significantly between endothelium-intact and endothelium-denuded preparations. Incubation with different potassium channel inhibitors (glibenclamide, 4-aminopyridine and tetraethylammonium) did not cause significant alterations in the relaxant responses to caffeine. These results suggest that the vasodilatory response to caffeine in human IMA is independent of endothelial function and is not mediated by potassium channels. © 2008 Elsevier Inc. All rights reserved.
publishDate 2009
dc.date.created.spa.fl_str_mv 2009
dc.date.accessioned.none.fl_str_mv 2020-05-26T00:02:18Z
dc.date.available.none.fl_str_mv 2020-05-26T00:02:18Z
dc.type.eng.fl_str_mv article
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.spa.spa.fl_str_mv Artículo
dc.identifier.doi.none.fl_str_mv https://doi.org/10.1016/j.vph.2008.11.002
dc.identifier.issn.none.fl_str_mv 15371891
dc.identifier.uri.none.fl_str_mv https://repository.urosario.edu.co/handle/10336/23470
url https://doi.org/10.1016/j.vph.2008.11.002
https://repository.urosario.edu.co/handle/10336/23470
identifier_str_mv 15371891
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.citationEndPage.none.fl_str_mv 136
dc.relation.citationIssue.none.fl_str_mv No. 43924
dc.relation.citationStartPage.none.fl_str_mv 132
dc.relation.citationTitle.none.fl_str_mv Vascular Pharmacology
dc.relation.citationVolume.none.fl_str_mv Vol. 50
dc.relation.ispartof.spa.fl_str_mv Vascular Pharmacology, ISSN:15371891, Vol.50, No.43924 (2009); pp. 132-136
dc.relation.uri.spa.fl_str_mv https://www.scopus.com/inward/record.uri?eid=2-s2.0-58549102308&doi=10.1016%2fj.vph.2008.11.002&partnerID=40&md5=64ba0d0bea4da031b615f07b7da23ff6
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.acceso.spa.fl_str_mv Abierto (Texto Completo)
rights_invalid_str_mv Abierto (Texto Completo)
http://purl.org/coar/access_right/c_abf2
dc.format.mimetype.none.fl_str_mv application/pdf
institution Universidad del Rosario
dc.source.instname.spa.fl_str_mv instname:Universidad del Rosario
dc.source.reponame.spa.fl_str_mv reponame:Repositorio Institucional EdocUR
repository.name.fl_str_mv Repositorio institucional EdocUR
repository.mail.fl_str_mv edocur@urosario.edu.co
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