Comparison of healing of full-thickness skin wounds grafted with multidirectional or unidirectional autologous artificial dermis: differential delivery of healing biomarkers

Cytokines, chemokines, and growth and remodeling factors orchestrate wound healing when skin damage occurs. During early stages, when the wound is still open, detection and quantification of these compounds might provide biomarkers of skin wound healing, which could aid to complete the scenario prov...

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Autores:
Tipo de recurso:
Fecha de publicación:
2018
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
eng
OAI Identifier:
oai:repository.urosario.edu.co:10336/23868
Acceso en línea:
https://doi.org/10.1007/s13346-018-0528-2
https://repository.urosario.edu.co/handle/10336/23868
Palabra clave:
Biological marker
Collagen type 1
Biological marker
Chemokine
Cytokine
Signal peptide
Animal cell
Animal experiment
Animal model
Animal tissue
Article
Comparative study
Controlled study
Follow up
Granulation tissue
Histopathology
Lagomorph
Morphometry
Nonhuman
Priority journal
Skin graft
Skin injury
Surface plasmon resonance
Wound closure
Wound fluid
Wound healing
Animal
Artificial skin
Autograft
Disease model
Epidermis
Metabolism
Skin disease
Skin transplantation
Tissue scaffold
Transplantation
Animals
Autografts
Biomarkers
Chemokines
Collagen type i
Cytokines
Epidermis
Intercellular signaling peptides and proteins
Skin diseases
Skin transplantation
Surface plasmon resonance
Tissue scaffolds
Wound healing
Differential delivery of bioactive compounds
Fiber orientation
Healing biomarkers
Healing of full-thickness skin wounds
Multidirectional and unidirectional autologous artificial dermis
Spr quantification
animal
artificial
Disease models
Skin
Rights
License
Abierto (Texto Completo)
Description
Summary:Cytokines, chemokines, and growth and remodeling factors orchestrate wound healing when skin damage occurs. During early stages, when the wound is still open, detection and quantification of these compounds might provide biomarkers of skin wound healing, which could aid to complete the scenario provided by clinical follow-up data and histological and histomorphometric analyses. This work assessed and compared the healing of full-thickness skin wounds grafted with artificial dermis made with autologous skin fibroblasts and unidirectional or multidirectional type I collagen scaffolds to test this hypothesis. Biomarkers of healing were detected and quantified in the culture medium of artificial dermis and exudates from the grafted wounds. Clinical follow-up of animals and histological and histomorphometric analysis showed differences in graft integration, wound closure, and histological and histomorphometric parameters. Surface plasmon resonance quantification of 13 healing biomarkers indicated differential secretion of most of the quantified factors in culture medium by the multidirectional and unidirectional artificial dermis. Also, there were significant differences between the concentration of some of the factors analyzed in the exudates of wounds grafted with the evaluated artificial dermis. These findings suggest that differential delivery of healing biomarkers induced by the directionality of the scaffold used to produce the multidirectional and unidirectional dermis was sufficient to create two skin wound microenvironments that determined a different outcome of healing. Overall, data indicate that healing of wounds grafted with multidirectional autologous artificial dermis is better than that of the wounds grafted with the unidirectional one. © 2018, Controlled Release Society.