Microscopical and elemental FESEM and Phenom ProX-SEM-EDS analysis of osteocyte- and blood vessel-like microstructures obtained from fossil vertebrates of the Eocene Messel Pit, Germany
The Eocene (?48 Ma) Messel Pit in Germany is a UNESCO World Heritage Site because of its exceptionally preserved fossils, including vertebrates, invertebrates, and plants. Messel fossil vertebrates are typically characterized by their articulated state, and in some cases the skin, hair, feathers, sc...
- Autores:
- Tipo de recurso:
- Fecha de publicación:
- 2016
- Institución:
- Universidad del Rosario
- Repositorio:
- Repositorio EdocUR - U. Rosario
- Idioma:
- eng
- OAI Identifier:
- oai:repository.urosario.edu.co:10336/28132
- Acceso en línea:
- https://doi.org/10.7717/peerj.1618
https://repository.urosario.edu.co/handle/10336/28132
- Palabra clave:
- Blood vessels
Crocodiles
Eocene
Germany
Mammals Messel Pit
Molecular paleontology
Osteocytes Turtles
- Rights
- License
- Abierto (Texto Completo)
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882350066002020-08-19T14:45:59Z2020-08-19T14:45:59Z2016-01-21The Eocene (?48 Ma) Messel Pit in Germany is a UNESCO World Heritage Site because of its exceptionally preserved fossils, including vertebrates, invertebrates, and plants. Messel fossil vertebrates are typically characterized by their articulated state, and in some cases the skin, hair, feathers, scales and stomach contents are also preserved. Despite the exceptional macroscopic preservation of Messel fossil vertebrates, the microstructural aspect of these fossils has been poorly explored. In particular, soft tissue structures such as hair or feathers have not been chemically analyzed, nor have bone microstructures. I report here the preservation and recovery of osteocyte-like and blood vessel-like microstructures from the bone of Messel Pit specimens, including the turtles Allaeochelys crassesculpta and Neochelys franzeni , the crocodile Diplocynodon darwini , and the pangolin Eomanis krebsi . I used a Field Emission Scanning Electron Microscope (FESEM) and a Phenom ProX desktop scanning electron microscope (LOT-QuantumDesign) equipped with a thermionic CeB6 source and a high sensitivity multi-mode backscatter electron (BSE) for microscopical and elemental characterization of these bone microstructures. Osteocyte-like and blood vessel-like microstructures are constituted by a thin layer (?50 nm thickness), external and internal mottled texture with slightly marked striations. Circular to linear marks are common on the external surface of the osteocyte-like microstructures and are interpreted as microbial troughs. Iron (Fe) is the most abundant element found in the osteocyte-like and blood vessel-like microstructures, but not in the bone matrix or collagen fibril-like microstructures. The occurrence of well-preserved soft-tissue elements (at least their physical form) establishes a promising background for future studies on preservation of biomolecules (proteins or DNA) in Messel Pit fossils.application/pdfhttps://doi.org/10.7717/peerj.1618ISSN: 2167-8359https://repository.urosario.edu.co/handle/10336/28132engPeerJe1618PeerJVol. 4PeerJ, ISSN: 2167-8359, VOL.4 (2016); PP. e1618https://peerj.com/articles/1618/Abierto (Texto Completo)http://purl.org/coar/access_right/c_abf2PeerJinstname:Universidad del Rosarioreponame:Repositorio Institucional EdocURBlood vesselsCrocodilesEoceneGermanyMammals Messel PitMolecular paleontologyOsteocytes TurtlesMicroscopical and elemental FESEM and Phenom ProX-SEM-EDS analysis of osteocyte- and blood vessel-like microstructures obtained from fossil vertebrates of the Eocene Messel Pit, GermanyAnálisis microscópico y elemental FESEM y Phenom ProX-SEM-EDS de microestructuras parecidas a osteocitos y vasos sanguíneos obtenidas de vertebrados fósiles del Eoceno Messel Pit, AlemaniaarticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Cadena, Edwin AlbertoORIGINALpeerj-1618.pdfapplication/pdf18153626https://repository.urosario.edu.co/bitstreams/cd0ec9a0-b6f2-449b-ad6e-db384b487a02/downloada7e5e125062175341f796e6df4c75d8bMD51TEXTpeerj-1618.pdf.txtpeerj-1618.pdf.txtExtracted texttext/plain35998https://repository.urosario.edu.co/bitstreams/f4f65476-6436-4d60-a891-cea4bc8083fb/downloadc80f798da2971fc240083daa7489742eMD52THUMBNAILpeerj-1618.pdf.jpgpeerj-1618.pdf.jpgGenerated Thumbnailimage/jpeg4373https://repository.urosario.edu.co/bitstreams/d71d577c-4e4d-4770-a337-5f0505a1ced7/download8912acb339130c5084c4329bc6848bb9MD5310336/28132oai:repository.urosario.edu.co:10336/281322021-01-21 03:18:53.649https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co |
dc.title.spa.fl_str_mv |
Microscopical and elemental FESEM and Phenom ProX-SEM-EDS analysis of osteocyte- and blood vessel-like microstructures obtained from fossil vertebrates of the Eocene Messel Pit, Germany |
dc.title.TranslatedTitle.spa.fl_str_mv |
Análisis microscópico y elemental FESEM y Phenom ProX-SEM-EDS de microestructuras parecidas a osteocitos y vasos sanguíneos obtenidas de vertebrados fósiles del Eoceno Messel Pit, Alemania |
title |
Microscopical and elemental FESEM and Phenom ProX-SEM-EDS analysis of osteocyte- and blood vessel-like microstructures obtained from fossil vertebrates of the Eocene Messel Pit, Germany |
spellingShingle |
Microscopical and elemental FESEM and Phenom ProX-SEM-EDS analysis of osteocyte- and blood vessel-like microstructures obtained from fossil vertebrates of the Eocene Messel Pit, Germany Blood vessels Crocodiles Eocene Germany Mammals Messel Pit Molecular paleontology Osteocytes Turtles |
title_short |
Microscopical and elemental FESEM and Phenom ProX-SEM-EDS analysis of osteocyte- and blood vessel-like microstructures obtained from fossil vertebrates of the Eocene Messel Pit, Germany |
title_full |
Microscopical and elemental FESEM and Phenom ProX-SEM-EDS analysis of osteocyte- and blood vessel-like microstructures obtained from fossil vertebrates of the Eocene Messel Pit, Germany |
title_fullStr |
Microscopical and elemental FESEM and Phenom ProX-SEM-EDS analysis of osteocyte- and blood vessel-like microstructures obtained from fossil vertebrates of the Eocene Messel Pit, Germany |
title_full_unstemmed |
Microscopical and elemental FESEM and Phenom ProX-SEM-EDS analysis of osteocyte- and blood vessel-like microstructures obtained from fossil vertebrates of the Eocene Messel Pit, Germany |
title_sort |
Microscopical and elemental FESEM and Phenom ProX-SEM-EDS analysis of osteocyte- and blood vessel-like microstructures obtained from fossil vertebrates of the Eocene Messel Pit, Germany |
dc.subject.keyword.spa.fl_str_mv |
Blood vessels Crocodiles Eocene Germany Mammals Messel Pit Molecular paleontology Osteocytes Turtles |
topic |
Blood vessels Crocodiles Eocene Germany Mammals Messel Pit Molecular paleontology Osteocytes Turtles |
description |
The Eocene (?48 Ma) Messel Pit in Germany is a UNESCO World Heritage Site because of its exceptionally preserved fossils, including vertebrates, invertebrates, and plants. Messel fossil vertebrates are typically characterized by their articulated state, and in some cases the skin, hair, feathers, scales and stomach contents are also preserved. Despite the exceptional macroscopic preservation of Messel fossil vertebrates, the microstructural aspect of these fossils has been poorly explored. In particular, soft tissue structures such as hair or feathers have not been chemically analyzed, nor have bone microstructures. I report here the preservation and recovery of osteocyte-like and blood vessel-like microstructures from the bone of Messel Pit specimens, including the turtles Allaeochelys crassesculpta and Neochelys franzeni , the crocodile Diplocynodon darwini , and the pangolin Eomanis krebsi . I used a Field Emission Scanning Electron Microscope (FESEM) and a Phenom ProX desktop scanning electron microscope (LOT-QuantumDesign) equipped with a thermionic CeB6 source and a high sensitivity multi-mode backscatter electron (BSE) for microscopical and elemental characterization of these bone microstructures. Osteocyte-like and blood vessel-like microstructures are constituted by a thin layer (?50 nm thickness), external and internal mottled texture with slightly marked striations. Circular to linear marks are common on the external surface of the osteocyte-like microstructures and are interpreted as microbial troughs. Iron (Fe) is the most abundant element found in the osteocyte-like and blood vessel-like microstructures, but not in the bone matrix or collagen fibril-like microstructures. The occurrence of well-preserved soft-tissue elements (at least their physical form) establishes a promising background for future studies on preservation of biomolecules (proteins or DNA) in Messel Pit fossils. |
publishDate |
2016 |
dc.date.created.spa.fl_str_mv |
2016-01-21 |
dc.date.accessioned.none.fl_str_mv |
2020-08-19T14:45:59Z |
dc.date.available.none.fl_str_mv |
2020-08-19T14:45:59Z |
dc.type.eng.fl_str_mv |
article |
dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
dc.type.spa.spa.fl_str_mv |
Artículo |
dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.7717/peerj.1618 |
dc.identifier.issn.none.fl_str_mv |
ISSN: 2167-8359 |
dc.identifier.uri.none.fl_str_mv |
https://repository.urosario.edu.co/handle/10336/28132 |
url |
https://doi.org/10.7717/peerj.1618 https://repository.urosario.edu.co/handle/10336/28132 |
identifier_str_mv |
ISSN: 2167-8359 |
dc.language.iso.spa.fl_str_mv |
eng |
language |
eng |
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e1618 |
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PeerJ |
dc.relation.citationVolume.none.fl_str_mv |
Vol. 4 |
dc.relation.ispartof.spa.fl_str_mv |
PeerJ, ISSN: 2167-8359, VOL.4 (2016); PP. e1618 |
dc.relation.uri.spa.fl_str_mv |
https://peerj.com/articles/1618/ |
dc.rights.coar.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
dc.rights.acceso.spa.fl_str_mv |
Abierto (Texto Completo) |
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Abierto (Texto Completo) http://purl.org/coar/access_right/c_abf2 |
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