Microscopical and elemental FESEM and Phenom ProX-SEM-EDS analysis of osteocyte- and blood vessel-like microstructures obtained from fossil vertebrates of the Eocene Messel Pit, Germany

The Eocene (?48 Ma) Messel Pit in Germany is a UNESCO World Heritage Site because of its exceptionally preserved fossils, including vertebrates, invertebrates, and plants. Messel fossil vertebrates are typically characterized by their articulated state, and in some cases the skin, hair, feathers, sc...

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Fecha de publicación:
2016
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
eng
OAI Identifier:
oai:repository.urosario.edu.co:10336/28132
Acceso en línea:
https://doi.org/10.7717/peerj.1618
https://repository.urosario.edu.co/handle/10336/28132
Palabra clave:
Blood vessels
Crocodiles
Eocene
Germany
Mammals Messel Pit
Molecular paleontology
Osteocytes Turtles
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License
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spelling 882350066002020-08-19T14:45:59Z2020-08-19T14:45:59Z2016-01-21The Eocene (?48 Ma) Messel Pit in Germany is a UNESCO World Heritage Site because of its exceptionally preserved fossils, including vertebrates, invertebrates, and plants. Messel fossil vertebrates are typically characterized by their articulated state, and in some cases the skin, hair, feathers, scales and stomach contents are also preserved. Despite the exceptional macroscopic preservation of Messel fossil vertebrates, the microstructural aspect of these fossils has been poorly explored. In particular, soft tissue structures such as hair or feathers have not been chemically analyzed, nor have bone microstructures. I report here the preservation and recovery of osteocyte-like and blood vessel-like microstructures from the bone of Messel Pit specimens, including the turtles Allaeochelys crassesculpta and Neochelys franzeni , the crocodile Diplocynodon darwini , and the pangolin Eomanis krebsi . I used a Field Emission Scanning Electron Microscope (FESEM) and a Phenom ProX desktop scanning electron microscope (LOT-QuantumDesign) equipped with a thermionic CeB6 source and a high sensitivity multi-mode backscatter electron (BSE) for microscopical and elemental characterization of these bone microstructures. Osteocyte-like and blood vessel-like microstructures are constituted by a thin layer (?50 nm thickness), external and internal mottled texture with slightly marked striations. Circular to linear marks are common on the external surface of the osteocyte-like microstructures and are interpreted as microbial troughs. Iron (Fe) is the most abundant element found in the osteocyte-like and blood vessel-like microstructures, but not in the bone matrix or collagen fibril-like microstructures. The occurrence of well-preserved soft-tissue elements (at least their physical form) establishes a promising background for future studies on preservation of biomolecules (proteins or DNA) in Messel Pit fossils.application/pdfhttps://doi.org/10.7717/peerj.1618ISSN: 2167-8359https://repository.urosario.edu.co/handle/10336/28132engPeerJe1618PeerJVol. 4PeerJ, ISSN: 2167-8359, VOL.4 (2016); PP. e1618https://peerj.com/articles/1618/Abierto (Texto Completo)http://purl.org/coar/access_right/c_abf2PeerJinstname:Universidad del Rosarioreponame:Repositorio Institucional EdocURBlood vesselsCrocodilesEoceneGermanyMammals Messel PitMolecular paleontologyOsteocytes TurtlesMicroscopical and elemental FESEM and Phenom ProX-SEM-EDS analysis of osteocyte- and blood vessel-like microstructures obtained from fossil vertebrates of the Eocene Messel Pit, GermanyAnálisis microscópico y elemental FESEM y Phenom ProX-SEM-EDS de microestructuras parecidas a osteocitos y vasos sanguíneos obtenidas de vertebrados fósiles del Eoceno Messel Pit, AlemaniaarticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Cadena, Edwin AlbertoORIGINALpeerj-1618.pdfapplication/pdf18153626https://repository.urosario.edu.co/bitstreams/cd0ec9a0-b6f2-449b-ad6e-db384b487a02/downloada7e5e125062175341f796e6df4c75d8bMD51TEXTpeerj-1618.pdf.txtpeerj-1618.pdf.txtExtracted texttext/plain35998https://repository.urosario.edu.co/bitstreams/f4f65476-6436-4d60-a891-cea4bc8083fb/downloadc80f798da2971fc240083daa7489742eMD52THUMBNAILpeerj-1618.pdf.jpgpeerj-1618.pdf.jpgGenerated Thumbnailimage/jpeg4373https://repository.urosario.edu.co/bitstreams/d71d577c-4e4d-4770-a337-5f0505a1ced7/download8912acb339130c5084c4329bc6848bb9MD5310336/28132oai:repository.urosario.edu.co:10336/281322021-01-21 03:18:53.649https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co
dc.title.spa.fl_str_mv Microscopical and elemental FESEM and Phenom ProX-SEM-EDS analysis of osteocyte- and blood vessel-like microstructures obtained from fossil vertebrates of the Eocene Messel Pit, Germany
dc.title.TranslatedTitle.spa.fl_str_mv Análisis microscópico y elemental FESEM y Phenom ProX-SEM-EDS de microestructuras parecidas a osteocitos y vasos sanguíneos obtenidas de vertebrados fósiles del Eoceno Messel Pit, Alemania
title Microscopical and elemental FESEM and Phenom ProX-SEM-EDS analysis of osteocyte- and blood vessel-like microstructures obtained from fossil vertebrates of the Eocene Messel Pit, Germany
spellingShingle Microscopical and elemental FESEM and Phenom ProX-SEM-EDS analysis of osteocyte- and blood vessel-like microstructures obtained from fossil vertebrates of the Eocene Messel Pit, Germany
Blood vessels
Crocodiles
Eocene
Germany
Mammals Messel Pit
Molecular paleontology
Osteocytes Turtles
title_short Microscopical and elemental FESEM and Phenom ProX-SEM-EDS analysis of osteocyte- and blood vessel-like microstructures obtained from fossil vertebrates of the Eocene Messel Pit, Germany
title_full Microscopical and elemental FESEM and Phenom ProX-SEM-EDS analysis of osteocyte- and blood vessel-like microstructures obtained from fossil vertebrates of the Eocene Messel Pit, Germany
title_fullStr Microscopical and elemental FESEM and Phenom ProX-SEM-EDS analysis of osteocyte- and blood vessel-like microstructures obtained from fossil vertebrates of the Eocene Messel Pit, Germany
title_full_unstemmed Microscopical and elemental FESEM and Phenom ProX-SEM-EDS analysis of osteocyte- and blood vessel-like microstructures obtained from fossil vertebrates of the Eocene Messel Pit, Germany
title_sort Microscopical and elemental FESEM and Phenom ProX-SEM-EDS analysis of osteocyte- and blood vessel-like microstructures obtained from fossil vertebrates of the Eocene Messel Pit, Germany
dc.subject.keyword.spa.fl_str_mv Blood vessels
Crocodiles
Eocene
Germany
Mammals Messel Pit
Molecular paleontology
Osteocytes Turtles
topic Blood vessels
Crocodiles
Eocene
Germany
Mammals Messel Pit
Molecular paleontology
Osteocytes Turtles
description The Eocene (?48 Ma) Messel Pit in Germany is a UNESCO World Heritage Site because of its exceptionally preserved fossils, including vertebrates, invertebrates, and plants. Messel fossil vertebrates are typically characterized by their articulated state, and in some cases the skin, hair, feathers, scales and stomach contents are also preserved. Despite the exceptional macroscopic preservation of Messel fossil vertebrates, the microstructural aspect of these fossils has been poorly explored. In particular, soft tissue structures such as hair or feathers have not been chemically analyzed, nor have bone microstructures. I report here the preservation and recovery of osteocyte-like and blood vessel-like microstructures from the bone of Messel Pit specimens, including the turtles Allaeochelys crassesculpta and Neochelys franzeni , the crocodile Diplocynodon darwini , and the pangolin Eomanis krebsi . I used a Field Emission Scanning Electron Microscope (FESEM) and a Phenom ProX desktop scanning electron microscope (LOT-QuantumDesign) equipped with a thermionic CeB6 source and a high sensitivity multi-mode backscatter electron (BSE) for microscopical and elemental characterization of these bone microstructures. Osteocyte-like and blood vessel-like microstructures are constituted by a thin layer (?50 nm thickness), external and internal mottled texture with slightly marked striations. Circular to linear marks are common on the external surface of the osteocyte-like microstructures and are interpreted as microbial troughs. Iron (Fe) is the most abundant element found in the osteocyte-like and blood vessel-like microstructures, but not in the bone matrix or collagen fibril-like microstructures. The occurrence of well-preserved soft-tissue elements (at least their physical form) establishes a promising background for future studies on preservation of biomolecules (proteins or DNA) in Messel Pit fossils.
publishDate 2016
dc.date.created.spa.fl_str_mv 2016-01-21
dc.date.accessioned.none.fl_str_mv 2020-08-19T14:45:59Z
dc.date.available.none.fl_str_mv 2020-08-19T14:45:59Z
dc.type.eng.fl_str_mv article
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dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.spa.spa.fl_str_mv Artículo
dc.identifier.doi.none.fl_str_mv https://doi.org/10.7717/peerj.1618
dc.identifier.issn.none.fl_str_mv ISSN: 2167-8359
dc.identifier.uri.none.fl_str_mv https://repository.urosario.edu.co/handle/10336/28132
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https://repository.urosario.edu.co/handle/10336/28132
identifier_str_mv ISSN: 2167-8359
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