Immunologic evaluation and validation of methods using synthetic peptides derived from Mycobacterium tuberculosis for the diagnosis of tuberculosis infection

The goal of this study was to demonstrate the usefulness of an enzyme-linked immunosorbent assay (ELISA) for the serodiagnosis of pulmonary tuberculosis (PTB) and extrapulmonary TB (EPTB). This assay used 20 amino acid-long, non-overlapped synthetic peptides that spanned the complete Mycobacterium t...

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Autores:
Tipo de recurso:
Fecha de publicación:
2013
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
eng
OAI Identifier:
oai:repository.urosario.edu.co:10336/21827
Acceso en línea:
https://doi.org/10.1590/0074-0276108022013001
https://repository.urosario.edu.co/handle/10336/21827
Palabra clave:
Incidencia & prevención de la enfermedad
Enfermedades
Pulmonary tuberculosis
Extrapulmonary tuberculosis
Synthetic peptide
ESAT-6 antigen
Ag85A antigen
Rights
License
Abierto (Texto Completo)
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oai_identifier_str oai:repository.urosario.edu.co:10336/21827
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network_name_str Repositorio EdocUR - U. Rosario
repository_id_str
dc.title.spa.fl_str_mv Immunologic evaluation and validation of methods using synthetic peptides derived from Mycobacterium tuberculosis for the diagnosis of tuberculosis infection
title Immunologic evaluation and validation of methods using synthetic peptides derived from Mycobacterium tuberculosis for the diagnosis of tuberculosis infection
spellingShingle Immunologic evaluation and validation of methods using synthetic peptides derived from Mycobacterium tuberculosis for the diagnosis of tuberculosis infection
Incidencia & prevención de la enfermedad
Enfermedades
Pulmonary tuberculosis
Extrapulmonary tuberculosis
Synthetic peptide
ESAT-6 antigen
Ag85A antigen
title_short Immunologic evaluation and validation of methods using synthetic peptides derived from Mycobacterium tuberculosis for the diagnosis of tuberculosis infection
title_full Immunologic evaluation and validation of methods using synthetic peptides derived from Mycobacterium tuberculosis for the diagnosis of tuberculosis infection
title_fullStr Immunologic evaluation and validation of methods using synthetic peptides derived from Mycobacterium tuberculosis for the diagnosis of tuberculosis infection
title_full_unstemmed Immunologic evaluation and validation of methods using synthetic peptides derived from Mycobacterium tuberculosis for the diagnosis of tuberculosis infection
title_sort Immunologic evaluation and validation of methods using synthetic peptides derived from Mycobacterium tuberculosis for the diagnosis of tuberculosis infection
dc.subject.ddc.spa.fl_str_mv Incidencia & prevención de la enfermedad
Enfermedades
topic Incidencia & prevención de la enfermedad
Enfermedades
Pulmonary tuberculosis
Extrapulmonary tuberculosis
Synthetic peptide
ESAT-6 antigen
Ag85A antigen
dc.subject.keyword.spa.fl_str_mv Pulmonary tuberculosis
Extrapulmonary tuberculosis
Synthetic peptide
ESAT-6 antigen
Ag85A antigen
description The goal of this study was to demonstrate the usefulness of an enzyme-linked immunosorbent assay (ELISA) for the serodiagnosis of pulmonary tuberculosis (PTB) and extrapulmonary TB (EPTB). This assay used 20 amino acid-long, non-overlapped synthetic peptides that spanned the complete Mycobacterium tuberculosis ESAT-6 and Ag85A sequences. The validation cohort consisted of 1,102 individuals who were grouped into the following five diagnostic groups: 455 patients with PTB, 60 patients with EPTB, 40 individuals with non-EPTB, 33 individuals with leprosy and 514 healthy controls. For the PTB group, two ESAT-6 peptides (12033 and 12034) had the highest sensitivity levels of 96.9% and 96.2%, respectively, and an Ag85A-peptide (29878) was the most specific (97.4%) in the PTB groups. For the EPTB group, two Ag85A peptides (11005 and 11006) were observed to have a sensitivity of 98.3% and an Ag85A-peptide (29878) was also the most specific (96.4%). When combinations of peptides were used, such as 12033 and 12034 or 11005 and 11006, 99.5% and 100% sensitivities in the PTB and EPTB groups were observed, respectively. In conclusion, for a cohort that consists entirely of individuals from Venezuela, a multi-antigen immunoassay using highly sensitive ESAT-6 and Ag85A peptides alone and in combination could be used to more rapidly diagnose PTB and EPTB infection.
publishDate 2013
dc.date.created.none.fl_str_mv 2013
dc.date.issued.none.fl_str_mv 2013
dc.date.accessioned.none.fl_str_mv 2020-04-29T16:59:03Z
dc.date.available.none.fl_str_mv 2020-04-29T16:59:03Z
dc.type.eng.fl_str_mv article
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dc.type.spa.spa.fl_str_mv Artículo
dc.identifier.doi.none.fl_str_mv https://doi.org/10.1590/0074-0276108022013001
dc.identifier.issn.none.fl_str_mv 0074-0276
dc.identifier.uri.none.fl_str_mv https://repository.urosario.edu.co/handle/10336/21827
url https://doi.org/10.1590/0074-0276108022013001
https://repository.urosario.edu.co/handle/10336/21827
identifier_str_mv 0074-0276
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.citationEndPage.none.fl_str_mv 139
dc.relation.citationIssue.none.fl_str_mv No. 2
dc.relation.citationStartPage.none.fl_str_mv 131
dc.relation.citationTitle.none.fl_str_mv Memorias do Instituto Oswaldo Cruz
dc.relation.citationVolume.none.fl_str_mv Vol. 108
dc.relation.ispartof.spa.fl_str_mv Memorias do Instituto Oswaldo Cruz, ISSN: 0074-0276 Vol. 108, No. 2 (2013) pp. 131-139
dc.relation.uri.spa.fl_str_mv http://www.scielo.br/pdf/mioc/v108n2/0074-0276-mioc-108-02-0131.pdf
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institution Universidad del Rosario
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spelling d599af7b-599f-469e-abc7-932873d01fc66006a3c0b63-65fc-46a3-a6cc-73f48f4b7bdc6007127924a-ee65-4678-a167-47bc9ef435d56009d0ceeb8-091f-4e9c-adc0-4259802b67a2600958e562d-b694-4e15-aff2-b2940fb71ddd600b0906016-9dfb-4e98-a9e7-4772ac44df3b600957dc3d0-8a63-46b2-a0a7-dec142a1e63b6000c5d20b3-361f-4517-af77-f5043634913260022d05efc-544e-49d6-873c-7ab10992bc8960043d29fbf-4180-47ae-9d74-50c4e388bcc96001f1322f2-9fb4-4783-917a-a5c99787cf4e60051721018600e5faf5ef-260a-4795-b5cc-4325df2600ed600796530656002020-04-29T16:59:03Z2020-04-29T16:59:03Z20132013The goal of this study was to demonstrate the usefulness of an enzyme-linked immunosorbent assay (ELISA) for the serodiagnosis of pulmonary tuberculosis (PTB) and extrapulmonary TB (EPTB). This assay used 20 amino acid-long, non-overlapped synthetic peptides that spanned the complete Mycobacterium tuberculosis ESAT-6 and Ag85A sequences. The validation cohort consisted of 1,102 individuals who were grouped into the following five diagnostic groups: 455 patients with PTB, 60 patients with EPTB, 40 individuals with non-EPTB, 33 individuals with leprosy and 514 healthy controls. For the PTB group, two ESAT-6 peptides (12033 and 12034) had the highest sensitivity levels of 96.9% and 96.2%, respectively, and an Ag85A-peptide (29878) was the most specific (97.4%) in the PTB groups. For the EPTB group, two Ag85A peptides (11005 and 11006) were observed to have a sensitivity of 98.3% and an Ag85A-peptide (29878) was also the most specific (96.4%). When combinations of peptides were used, such as 12033 and 12034 or 11005 and 11006, 99.5% and 100% sensitivities in the PTB and EPTB groups were observed, respectively. In conclusion, for a cohort that consists entirely of individuals from Venezuela, a multi-antigen immunoassay using highly sensitive ESAT-6 and Ag85A peptides alone and in combination could be used to more rapidly diagnose PTB and EPTB infection.application/pdfhttps://doi.org/10.1590/0074-02761080220130010074-0276https://repository.urosario.edu.co/handle/10336/21827eng139No. 2131Memorias do Instituto Oswaldo CruzVol. 108Memorias do Instituto Oswaldo Cruz, ISSN: 0074-0276 Vol. 108, No. 2 (2013) pp. 131-139http://www.scielo.br/pdf/mioc/v108n2/0074-0276-mioc-108-02-0131.pdfAbierto (Texto Completo)http://purl.org/coar/access_right/c_abf2instname:Universidad del Rosarioreponame:Repositorio Institucional EdocURIncidencia & prevención de la enfermedad614600Enfermedades616600Pulmonary tuberculosisExtrapulmonary tuberculosisSynthetic peptideESAT-6 antigenAg85A antigenImmunologic evaluation and validation of methods using synthetic peptides derived from Mycobacterium tuberculosis for the diagnosis of tuberculosis infectionarticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Araujo, ZaidaGiampietro, FrancescaBochichio, María de los AngelesPalacios, AndreaDinis, JeniferIsern, Jaimede Waard, Jacobus H.Rada, ElsaBorges, RafaelLarrea, Carlos Fernández deVillasmil, AngelVanegas, MagnoliaEnciso-Moreno, Jose AntonioPatarroyo, Manuel A.Araujo, ZaidaGiampietro, FrancescaBochichio, María de los ÁngelesPalacios, AndreaDinis, JeniferIsern, JaimeWaard, Jacobus Henry deRada, ElsaBorges, RafaelLarrea, Carlos Fernández deVillasmil, AngelVanegas, MagnoliaEnciso-Moreno, Jose AntonioPatarroyo, Manuel AlfonsoORIGINALImmunologic_evaluation_and_validation_of_methods.pdfapplication/pdf585593https://repository.urosario.edu.co/bitstreams/6cfedc33-700b-478f-a7f9-3aae1465c918/downloadf828b544529b4a2c3a76836eb4f13874MD51TEXTImmunologic_evaluation_and_validation_of_methods.pdf.txtImmunologic_evaluation_and_validation_of_methods.pdf.txtExtracted texttext/plain48100https://repository.urosario.edu.co/bitstreams/06e9f978-f803-4abc-a028-527d45780bd0/download529c41d98396c9aea75bf2d3776b0e9bMD52THUMBNAILImmunologic_evaluation_and_validation_of_methods.pdf.jpgImmunologic_evaluation_and_validation_of_methods.pdf.jpgGenerated Thumbnailimage/jpeg4749https://repository.urosario.edu.co/bitstreams/d8a75720-245d-472c-ac0f-19e7339dbfbe/download95eba600a973f1044ccf5dd6202d0d6eMD5310336/21827oai:repository.urosario.edu.co:10336/218272020-05-13 14:49:45.301https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co