Immunologic evaluation and validation of methods using synthetic peptides derived from Mycobacterium tuberculosis for the diagnosis of tuberculosis infection
The goal of this study was to demonstrate the usefulness of an enzyme-linked immunosorbent assay (ELISA) for the serodiagnosis of pulmonary tuberculosis (PTB) and extrapulmonary TB (EPTB). This assay used 20 amino acid-long, non-overlapped synthetic peptides that spanned the complete Mycobacterium t...
- Autores:
- Tipo de recurso:
- Fecha de publicación:
- 2013
- Institución:
- Universidad del Rosario
- Repositorio:
- Repositorio EdocUR - U. Rosario
- Idioma:
- eng
- OAI Identifier:
- oai:repository.urosario.edu.co:10336/21827
- Acceso en línea:
- https://doi.org/10.1590/0074-0276108022013001
https://repository.urosario.edu.co/handle/10336/21827
- Palabra clave:
- Incidencia & prevención de la enfermedad
Enfermedades
Pulmonary tuberculosis
Extrapulmonary tuberculosis
Synthetic peptide
ESAT-6 antigen
Ag85A antigen
- Rights
- License
- Abierto (Texto Completo)
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oai:repository.urosario.edu.co:10336/21827 |
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Repositorio EdocUR - U. Rosario |
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|
dc.title.spa.fl_str_mv |
Immunologic evaluation and validation of methods using synthetic peptides derived from Mycobacterium tuberculosis for the diagnosis of tuberculosis infection |
title |
Immunologic evaluation and validation of methods using synthetic peptides derived from Mycobacterium tuberculosis for the diagnosis of tuberculosis infection |
spellingShingle |
Immunologic evaluation and validation of methods using synthetic peptides derived from Mycobacterium tuberculosis for the diagnosis of tuberculosis infection Incidencia & prevención de la enfermedad Enfermedades Pulmonary tuberculosis Extrapulmonary tuberculosis Synthetic peptide ESAT-6 antigen Ag85A antigen |
title_short |
Immunologic evaluation and validation of methods using synthetic peptides derived from Mycobacterium tuberculosis for the diagnosis of tuberculosis infection |
title_full |
Immunologic evaluation and validation of methods using synthetic peptides derived from Mycobacterium tuberculosis for the diagnosis of tuberculosis infection |
title_fullStr |
Immunologic evaluation and validation of methods using synthetic peptides derived from Mycobacterium tuberculosis for the diagnosis of tuberculosis infection |
title_full_unstemmed |
Immunologic evaluation and validation of methods using synthetic peptides derived from Mycobacterium tuberculosis for the diagnosis of tuberculosis infection |
title_sort |
Immunologic evaluation and validation of methods using synthetic peptides derived from Mycobacterium tuberculosis for the diagnosis of tuberculosis infection |
dc.subject.ddc.spa.fl_str_mv |
Incidencia & prevención de la enfermedad Enfermedades |
topic |
Incidencia & prevención de la enfermedad Enfermedades Pulmonary tuberculosis Extrapulmonary tuberculosis Synthetic peptide ESAT-6 antigen Ag85A antigen |
dc.subject.keyword.spa.fl_str_mv |
Pulmonary tuberculosis Extrapulmonary tuberculosis Synthetic peptide ESAT-6 antigen Ag85A antigen |
description |
The goal of this study was to demonstrate the usefulness of an enzyme-linked immunosorbent assay (ELISA) for the serodiagnosis of pulmonary tuberculosis (PTB) and extrapulmonary TB (EPTB). This assay used 20 amino acid-long, non-overlapped synthetic peptides that spanned the complete Mycobacterium tuberculosis ESAT-6 and Ag85A sequences. The validation cohort consisted of 1,102 individuals who were grouped into the following five diagnostic groups: 455 patients with PTB, 60 patients with EPTB, 40 individuals with non-EPTB, 33 individuals with leprosy and 514 healthy controls. For the PTB group, two ESAT-6 peptides (12033 and 12034) had the highest sensitivity levels of 96.9% and 96.2%, respectively, and an Ag85A-peptide (29878) was the most specific (97.4%) in the PTB groups. For the EPTB group, two Ag85A peptides (11005 and 11006) were observed to have a sensitivity of 98.3% and an Ag85A-peptide (29878) was also the most specific (96.4%). When combinations of peptides were used, such as 12033 and 12034 or 11005 and 11006, 99.5% and 100% sensitivities in the PTB and EPTB groups were observed, respectively. In conclusion, for a cohort that consists entirely of individuals from Venezuela, a multi-antigen immunoassay using highly sensitive ESAT-6 and Ag85A peptides alone and in combination could be used to more rapidly diagnose PTB and EPTB infection. |
publishDate |
2013 |
dc.date.created.none.fl_str_mv |
2013 |
dc.date.issued.none.fl_str_mv |
2013 |
dc.date.accessioned.none.fl_str_mv |
2020-04-29T16:59:03Z |
dc.date.available.none.fl_str_mv |
2020-04-29T16:59:03Z |
dc.type.eng.fl_str_mv |
article |
dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
dc.type.spa.spa.fl_str_mv |
Artículo |
dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.1590/0074-0276108022013001 |
dc.identifier.issn.none.fl_str_mv |
0074-0276 |
dc.identifier.uri.none.fl_str_mv |
https://repository.urosario.edu.co/handle/10336/21827 |
url |
https://doi.org/10.1590/0074-0276108022013001 https://repository.urosario.edu.co/handle/10336/21827 |
identifier_str_mv |
0074-0276 |
dc.language.iso.spa.fl_str_mv |
eng |
language |
eng |
dc.relation.citationEndPage.none.fl_str_mv |
139 |
dc.relation.citationIssue.none.fl_str_mv |
No. 2 |
dc.relation.citationStartPage.none.fl_str_mv |
131 |
dc.relation.citationTitle.none.fl_str_mv |
Memorias do Instituto Oswaldo Cruz |
dc.relation.citationVolume.none.fl_str_mv |
Vol. 108 |
dc.relation.ispartof.spa.fl_str_mv |
Memorias do Instituto Oswaldo Cruz, ISSN: 0074-0276 Vol. 108, No. 2 (2013) pp. 131-139 |
dc.relation.uri.spa.fl_str_mv |
http://www.scielo.br/pdf/mioc/v108n2/0074-0276-mioc-108-02-0131.pdf |
dc.rights.coar.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
dc.rights.acceso.spa.fl_str_mv |
Abierto (Texto Completo) |
rights_invalid_str_mv |
Abierto (Texto Completo) http://purl.org/coar/access_right/c_abf2 |
dc.format.mimetype.none.fl_str_mv |
application/pdf |
institution |
Universidad del Rosario |
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dc.source.reponame.none.fl_str_mv |
reponame:Repositorio Institucional EdocUR |
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d599af7b-599f-469e-abc7-932873d01fc66006a3c0b63-65fc-46a3-a6cc-73f48f4b7bdc6007127924a-ee65-4678-a167-47bc9ef435d56009d0ceeb8-091f-4e9c-adc0-4259802b67a2600958e562d-b694-4e15-aff2-b2940fb71ddd600b0906016-9dfb-4e98-a9e7-4772ac44df3b600957dc3d0-8a63-46b2-a0a7-dec142a1e63b6000c5d20b3-361f-4517-af77-f5043634913260022d05efc-544e-49d6-873c-7ab10992bc8960043d29fbf-4180-47ae-9d74-50c4e388bcc96001f1322f2-9fb4-4783-917a-a5c99787cf4e60051721018600e5faf5ef-260a-4795-b5cc-4325df2600ed600796530656002020-04-29T16:59:03Z2020-04-29T16:59:03Z20132013The goal of this study was to demonstrate the usefulness of an enzyme-linked immunosorbent assay (ELISA) for the serodiagnosis of pulmonary tuberculosis (PTB) and extrapulmonary TB (EPTB). This assay used 20 amino acid-long, non-overlapped synthetic peptides that spanned the complete Mycobacterium tuberculosis ESAT-6 and Ag85A sequences. The validation cohort consisted of 1,102 individuals who were grouped into the following five diagnostic groups: 455 patients with PTB, 60 patients with EPTB, 40 individuals with non-EPTB, 33 individuals with leprosy and 514 healthy controls. For the PTB group, two ESAT-6 peptides (12033 and 12034) had the highest sensitivity levels of 96.9% and 96.2%, respectively, and an Ag85A-peptide (29878) was the most specific (97.4%) in the PTB groups. For the EPTB group, two Ag85A peptides (11005 and 11006) were observed to have a sensitivity of 98.3% and an Ag85A-peptide (29878) was also the most specific (96.4%). When combinations of peptides were used, such as 12033 and 12034 or 11005 and 11006, 99.5% and 100% sensitivities in the PTB and EPTB groups were observed, respectively. In conclusion, for a cohort that consists entirely of individuals from Venezuela, a multi-antigen immunoassay using highly sensitive ESAT-6 and Ag85A peptides alone and in combination could be used to more rapidly diagnose PTB and EPTB infection.application/pdfhttps://doi.org/10.1590/0074-02761080220130010074-0276https://repository.urosario.edu.co/handle/10336/21827eng139No. 2131Memorias do Instituto Oswaldo CruzVol. 108Memorias do Instituto Oswaldo Cruz, ISSN: 0074-0276 Vol. 108, No. 2 (2013) pp. 131-139http://www.scielo.br/pdf/mioc/v108n2/0074-0276-mioc-108-02-0131.pdfAbierto (Texto Completo)http://purl.org/coar/access_right/c_abf2instname:Universidad del Rosarioreponame:Repositorio Institucional EdocURIncidencia & prevención de la enfermedad614600Enfermedades616600Pulmonary tuberculosisExtrapulmonary tuberculosisSynthetic peptideESAT-6 antigenAg85A antigenImmunologic evaluation and validation of methods using synthetic peptides derived from Mycobacterium tuberculosis for the diagnosis of tuberculosis infectionarticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Araujo, ZaidaGiampietro, FrancescaBochichio, María de los AngelesPalacios, AndreaDinis, JeniferIsern, Jaimede Waard, Jacobus H.Rada, ElsaBorges, RafaelLarrea, Carlos Fernández deVillasmil, AngelVanegas, MagnoliaEnciso-Moreno, Jose AntonioPatarroyo, Manuel A.Araujo, ZaidaGiampietro, FrancescaBochichio, María de los ÁngelesPalacios, AndreaDinis, JeniferIsern, JaimeWaard, Jacobus Henry deRada, ElsaBorges, RafaelLarrea, Carlos Fernández deVillasmil, AngelVanegas, MagnoliaEnciso-Moreno, Jose AntonioPatarroyo, Manuel AlfonsoORIGINALImmunologic_evaluation_and_validation_of_methods.pdfapplication/pdf585593https://repository.urosario.edu.co/bitstreams/6cfedc33-700b-478f-a7f9-3aae1465c918/downloadf828b544529b4a2c3a76836eb4f13874MD51TEXTImmunologic_evaluation_and_validation_of_methods.pdf.txtImmunologic_evaluation_and_validation_of_methods.pdf.txtExtracted texttext/plain48100https://repository.urosario.edu.co/bitstreams/06e9f978-f803-4abc-a028-527d45780bd0/download529c41d98396c9aea75bf2d3776b0e9bMD52THUMBNAILImmunologic_evaluation_and_validation_of_methods.pdf.jpgImmunologic_evaluation_and_validation_of_methods.pdf.jpgGenerated Thumbnailimage/jpeg4749https://repository.urosario.edu.co/bitstreams/d8a75720-245d-472c-ac0f-19e7339dbfbe/download95eba600a973f1044ccf5dd6202d0d6eMD5310336/21827oai:repository.urosario.edu.co:10336/218272020-05-13 14:49:45.301https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co |