The eukaryotic genome, its reads, and the unfinished assembly

In recent years, readily affordable short read sequences provided by next-generation sequencing (NGS) have become longer and more accurate. This has led to a jump in interest in the utility of NGS-only approaches for exploring eukaryotic genomes. The concept of a static, 'finished' genome assembly, which still appears to be a faraway goal for many eukaryotes, is yielding to new paradigms. We here motivate an object-view concept where the raw reads are the main, fixed object, and assemblies with their annotations take a role of dynamically changing and modifiable views of that object. © 2013 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

• Fecha de publicación: 2013
• Institución: Universidad del Rosario
• Repositorio: Repositorio EdocUR - U. Rosario
• Idioma: eng
• OAI Identifier: oai:repository.urosario.edu.co:10336/24056
• Acceso en línea: https://doi.org/10.1016/j.febslet.2013.05.048; https://repository.urosario.edu.co/handle/10336/24056
• Palabra clave: Accuracy; Algorithm; Article; Bioinformatics; Fungal genome; Fungal strain; Genome analysis; Human; Information processing; Information retrieval; Next generation sequencing; Nonhuman; Priority journal; Reliability; Reproducibility; Sequence analysis; Animals; Base sequence; Chromosome mapping; Eukaryota; Genomics; High-throughput nucleotide sequencing; Humans; Molecular sequence annotation; Eukaryota; Assembly-free genome analysis; Eukaryotic genomics; Microbial strain collection; Next generation sequencing; Object-view separation; dna; human; Genome; Sequence analysis
• License: Abierto (Texto Completo)