Functional, biochemical and 3D studies of Mycobacterium tuberculosis protein peptides for an effective anti-tuberculosis vaccine

Tuberculosis (TB) is an air-born, transmissible disease, having an estimated 9.4 million new TB cases worldwide in 2009. Eventual control of this disease by developing a safe and efficient new vaccine able to detain its spread will have an enormous impact on public health policy. Selecting potential antigens to be included in a multi-epitope, minimal subunit-based, chemically-synthesized vaccine containing the minimum sequences needed for blocking mycobacterial interaction with host cells is a complex task due to the multiple mechanisms involved in M. tuberculosis infection and the mycobacterium's immune evasion mechanisms. Our methodology, described here takes into account a highly robust, specific, sensitive and functional approach to the search for potential epitopes to be included in an anti-TB vaccine; it has been based on identifying short mycobacterial protein fragments using synthetic peptides having high affinity interaction with alveolar epithelial cells (A549) and monocyte-derived macrophages (U937) which are able to block the microorganism's entry to target cells in in vitro assays. This manuscript presents a review of the results obtained with some of the MTB H37Rv proteins studied to date, aimed at using these high activity binding peptides (HABPs) as platforms to be included in a minimal subunit-based, multiepitope, chemically-synthesized, antituberculosis vaccine. © 2014 Informa Healthcare USA, Inc. All rights reserved: reproduction in whole or part not permitted.

• Fecha de publicación: 2014
• Institución: Universidad del Rosario
• Repositorio: Repositorio EdocUR - U. Rosario
• Idioma: eng
• OAI Identifier: oai:repository.urosario.edu.co:10336/23782
• Acceso en línea: https://doi.org/10.3109/1040841X.2013.763221; https://repository.urosario.edu.co/handle/10336/23782
• Palabra clave: Bacterial antigen; Bacterial protein; Bcg vaccine; Rv 0180 c protein; Rv 0227 c protein; Rv 0679 c protein; Rv 1490 protein; Rv 1510 c protein; Rv 1980 c protein; Rv 2004 c protein; Rv 2301 protein; Rv 2536 protein; Rv 2560 protein; Rv 2707 protein; Rv 2969c protein; Rv 3166 c protein; Rv 3481 c protein; Rv 3629 c protein; Rv 3630 protein; Rv 3804 c protein; Rv 3910 protein; Synthetic peptide; Unclassified drug; Bioinformatics; Immune response; Molecular biology; Mycobacterium tuberculosis; Nonhuman; Priority journal; Protein analysis; Protein binding; Protein function; Proteomics; Review; Three dimensional imaging; Bacterial adhesion; Bacterial proteins; Cell line; Epithelial cells; Humans; Macrophages; Mycobacterium tuberculosis; Tuberculosis vaccines; Mycobacterium tuberculosis surface protein; Receptor-ligand interaction; Subunit-vaccine; Synthetic-peptides; bacterial; Antigens
• License: Abierto (Texto Completo)