Peptides of the liver stage antigen-1 (LSA-1) of Plasmodium falciparum bind to human hepatocytes

Synthetic peptides from the liver stage antigen-1 (LSA-1) antigen sequence were used in HepG2 cell and erythrocyte binding assays to identify regiones thar could be involuved in parasite invesion. LSA-1 protein peptides 20630 ( 21 INGKIIKNSEKDEIIKSNLRY 40), 20637 (157 KEKLQGQQSDSEQERRAY 173), 20638 (174 KEKLQEQQSDLEQERLA 190) and 20639 (191KEKLQEQQSDLEQERRAY 207) had high binding activity in HepG2 assays. Were located in immunogenic regions; peptides cell binding was saturable. Peptide 20630 bound specifically to 48 kDa HepG2 membrane surface protein. LSA-1 peptides 20630 (21 INGKIIKNSEKDEIIKSNLRY 40) and 20633 (81 DKELTMSNVKNVSQTNFSLY 100) showed specific erythrocyte binding activity and inhibited merozoite invasion of erythrocytes in vitro. A monkey serum prepared against LSA-1 20630 peptide analog (CGINGKNIKNAEKPMIIKSNLRGC) inhibited merozoite invasion in vitro. The data suggest LSA-1 “High Activity Binding Peptides” could play a possible role in hepatic cell invasion as well as merozoite invasion of erythrocytes.

• Fecha de publicación: 2003
• Institución: Universidad del Rosario
• Repositorio: Repositorio EdocUR - U. Rosario
• Idioma: eng
• OAI Identifier: oai:repository.urosario.edu.co:10336/26704
• Acceso en línea: https://doi.org/10.1016/S0196-9781(03)00135-9; https://repository.urosario.edu.co/handle/10336/26704
• Palabra clave: P. falciparum; Critical residues; Liver stage antigen (LSA); High activity binding peptides (HABPs); Phosphate buffered saline (PBS); Red blood cells (RBC)
• License: Restringido (Acceso a grupos específicos)