Interaction of leishmania (L.) chagasi with the lulo cell line in different environmental conditions

There are unknown or poorly understood events in the Leishmania-host cell interactions that merit studies in new in vitro models. The aim of the present work was to evaluate Leishmania chagasi interaction with a cell line (Lulo) previously established and derived from Lutzomyia longipalpis embryonic...

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Fecha de publicación:
2006
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
spa
OAI Identifier:
oai:repository.urosario.edu.co:10336/23007
Acceso en línea:
https://repository.urosario.edu.co/handle/10336/23007
Palabra clave:
Leishmania donovani chagasi
Lutzomyia longipalpis
Solanum quitoense
Cell morphometry
Percentage of infection
Promastigote
Temperature and co 2
Ultrastructure characteristics
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spelling 34b63110-6888-4bba-9cc8-6f075fc56dc7-19253ba68-86a7-4501-a31d-369280ee7264-1efa9d9a3-26df-4963-bccd-9e23d1b87159-19b72ba46-b2f4-424d-a5cd-8bea16665306-12722bfca-f932-4999-bfdd-5b5808beaa28-12020-05-25T23:59:13Z2020-05-25T23:59:13Z2006There are unknown or poorly understood events in the Leishmania-host cell interactions that merit studies in new in vitro models. The aim of the present work was to evaluate Leishmania chagasi interaction with a cell line (Lulo) previously established and derived from Lutzomyia longipalpis embryonic tissues, bearing different temperatures and CO 2 concentrations in mind and morphometric changes of the cells. The J774 cell line was used as positive control of the infection. The L. chagasi MH/CO/84/CL-044B strain was used for infection assays. The parasites were added to adherent cells, in Lulo as well as J774, in a 5:1 ratio. Incubation was at 28°C and 37°C, in the absence and presence of CO 2. A morphometric study of the cells was carried out before and after infection. Both parasite and cell ultrastructure characteristics were analyzed by electron microscope. The greatest percentage of infection in the Lulo cells at 28°C (26,8%) was registered on day six post infection, while at 37°C, in the absence of CO 2, the maximum value (30,4%) was obtained on day nine. The morphometric analyses showed a significant increase in the size of Lulo cells infected at 28°C and J774 cells at 37°C with and without CO 2, respectively.application/pdf1200488https://repository.urosario.edu.co/handle/10336/23007spa171No. 2165Revista Colombiana de EntomologiaVol. 32Revista Colombiana de Entomologia, ISSN:1200488, Vol.32, No.2 (2006); pp. 165-171https://www.scopus.com/inward/record.uri?eid=2-s2.0-79960469168&partnerID=40&md5=3c70dbc529752606952e463511204368Abierto (Texto Completo)http://purl.org/coar/access_right/c_abf2instname:Universidad del Rosarioreponame:Repositorio Institucional EdocURLeishmania donovani chagasiLutzomyia longipalpisSolanum quitoenseCell morphometryPercentage of infectionPromastigoteTemperature and co 2Ultrastructure characteristicsInteraction of leishmania (L.) chagasi with the lulo cell line in different environmental conditionsInteracción de leishmania (L.) chagasi con la línea celular lulo en diferentes condiciones ambientalesarticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Acero V.Galeano E.Ayala M.Castellanos J.Bello F.ORIGINALv32n2a10.pdfapplication/pdf560990https://repository.urosario.edu.co/bitstreams/a4eaf40f-a8d5-4f9b-9656-2e1431b07de6/downloadaf0fdfac54ed991e5f995d8a05f5a34dMD51TEXTv32n2a10.pdf.txtv32n2a10.pdf.txtExtracted texttext/plain45496https://repository.urosario.edu.co/bitstreams/43323365-a153-40be-8801-07acebd4481d/download8d4420caac029f7345b5b45a1944b3ddMD52THUMBNAILv32n2a10.pdf.jpgv32n2a10.pdf.jpgGenerated Thumbnailimage/jpeg4561https://repository.urosario.edu.co/bitstreams/d2302742-ee63-4474-b102-22b343adaabf/download49bf9a354dc4140c30cfb2a1d4af8becMD5310336/23007oai:repository.urosario.edu.co:10336/230072022-05-02 07:37:14.438998https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co
dc.title.spa.fl_str_mv Interaction of leishmania (L.) chagasi with the lulo cell line in different environmental conditions
dc.title.TranslatedTitle.spa.fl_str_mv Interacción de leishmania (L.) chagasi con la línea celular lulo en diferentes condiciones ambientales
title Interaction of leishmania (L.) chagasi with the lulo cell line in different environmental conditions
spellingShingle Interaction of leishmania (L.) chagasi with the lulo cell line in different environmental conditions
Leishmania donovani chagasi
Lutzomyia longipalpis
Solanum quitoense
Cell morphometry
Percentage of infection
Promastigote
Temperature and co 2
Ultrastructure characteristics
title_short Interaction of leishmania (L.) chagasi with the lulo cell line in different environmental conditions
title_full Interaction of leishmania (L.) chagasi with the lulo cell line in different environmental conditions
title_fullStr Interaction of leishmania (L.) chagasi with the lulo cell line in different environmental conditions
title_full_unstemmed Interaction of leishmania (L.) chagasi with the lulo cell line in different environmental conditions
title_sort Interaction of leishmania (L.) chagasi with the lulo cell line in different environmental conditions
dc.subject.keyword.spa.fl_str_mv Leishmania donovani chagasi
Lutzomyia longipalpis
Solanum quitoense
Cell morphometry
Percentage of infection
Promastigote
Temperature and co 2
Ultrastructure characteristics
topic Leishmania donovani chagasi
Lutzomyia longipalpis
Solanum quitoense
Cell morphometry
Percentage of infection
Promastigote
Temperature and co 2
Ultrastructure characteristics
description There are unknown or poorly understood events in the Leishmania-host cell interactions that merit studies in new in vitro models. The aim of the present work was to evaluate Leishmania chagasi interaction with a cell line (Lulo) previously established and derived from Lutzomyia longipalpis embryonic tissues, bearing different temperatures and CO 2 concentrations in mind and morphometric changes of the cells. The J774 cell line was used as positive control of the infection. The L. chagasi MH/CO/84/CL-044B strain was used for infection assays. The parasites were added to adherent cells, in Lulo as well as J774, in a 5:1 ratio. Incubation was at 28°C and 37°C, in the absence and presence of CO 2. A morphometric study of the cells was carried out before and after infection. Both parasite and cell ultrastructure characteristics were analyzed by electron microscope. The greatest percentage of infection in the Lulo cells at 28°C (26,8%) was registered on day six post infection, while at 37°C, in the absence of CO 2, the maximum value (30,4%) was obtained on day nine. The morphometric analyses showed a significant increase in the size of Lulo cells infected at 28°C and J774 cells at 37°C with and without CO 2, respectively.
publishDate 2006
dc.date.created.spa.fl_str_mv 2006
dc.date.accessioned.none.fl_str_mv 2020-05-25T23:59:13Z
dc.date.available.none.fl_str_mv 2020-05-25T23:59:13Z
dc.type.eng.fl_str_mv article
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dc.type.spa.spa.fl_str_mv Artículo
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dc.identifier.uri.none.fl_str_mv https://repository.urosario.edu.co/handle/10336/23007
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dc.language.iso.spa.fl_str_mv spa
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dc.relation.citationEndPage.none.fl_str_mv 171
dc.relation.citationIssue.none.fl_str_mv No. 2
dc.relation.citationStartPage.none.fl_str_mv 165
dc.relation.citationTitle.none.fl_str_mv Revista Colombiana de Entomologia
dc.relation.citationVolume.none.fl_str_mv Vol. 32
dc.relation.ispartof.spa.fl_str_mv Revista Colombiana de Entomologia, ISSN:1200488, Vol.32, No.2 (2006); pp. 165-171
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