Interaction of leishmania (L.) chagasi with the lulo cell line in different environmental conditions
There are unknown or poorly understood events in the Leishmania-host cell interactions that merit studies in new in vitro models. The aim of the present work was to evaluate Leishmania chagasi interaction with a cell line (Lulo) previously established and derived from Lutzomyia longipalpis embryonic...
- Autores:
- Tipo de recurso:
- Fecha de publicación:
- 2006
- Institución:
- Universidad del Rosario
- Repositorio:
- Repositorio EdocUR - U. Rosario
- Idioma:
- spa
- OAI Identifier:
- oai:repository.urosario.edu.co:10336/23007
- Acceso en línea:
- https://repository.urosario.edu.co/handle/10336/23007
- Palabra clave:
- Leishmania donovani chagasi
Lutzomyia longipalpis
Solanum quitoense
Cell morphometry
Percentage of infection
Promastigote
Temperature and co 2
Ultrastructure characteristics
- Rights
- License
- Abierto (Texto Completo)
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34b63110-6888-4bba-9cc8-6f075fc56dc7-19253ba68-86a7-4501-a31d-369280ee7264-1efa9d9a3-26df-4963-bccd-9e23d1b87159-19b72ba46-b2f4-424d-a5cd-8bea16665306-12722bfca-f932-4999-bfdd-5b5808beaa28-12020-05-25T23:59:13Z2020-05-25T23:59:13Z2006There are unknown or poorly understood events in the Leishmania-host cell interactions that merit studies in new in vitro models. The aim of the present work was to evaluate Leishmania chagasi interaction with a cell line (Lulo) previously established and derived from Lutzomyia longipalpis embryonic tissues, bearing different temperatures and CO 2 concentrations in mind and morphometric changes of the cells. The J774 cell line was used as positive control of the infection. The L. chagasi MH/CO/84/CL-044B strain was used for infection assays. The parasites were added to adherent cells, in Lulo as well as J774, in a 5:1 ratio. Incubation was at 28°C and 37°C, in the absence and presence of CO 2. A morphometric study of the cells was carried out before and after infection. Both parasite and cell ultrastructure characteristics were analyzed by electron microscope. The greatest percentage of infection in the Lulo cells at 28°C (26,8%) was registered on day six post infection, while at 37°C, in the absence of CO 2, the maximum value (30,4%) was obtained on day nine. The morphometric analyses showed a significant increase in the size of Lulo cells infected at 28°C and J774 cells at 37°C with and without CO 2, respectively.application/pdf1200488https://repository.urosario.edu.co/handle/10336/23007spa171No. 2165Revista Colombiana de EntomologiaVol. 32Revista Colombiana de Entomologia, ISSN:1200488, Vol.32, No.2 (2006); pp. 165-171https://www.scopus.com/inward/record.uri?eid=2-s2.0-79960469168&partnerID=40&md5=3c70dbc529752606952e463511204368Abierto (Texto Completo)http://purl.org/coar/access_right/c_abf2instname:Universidad del Rosarioreponame:Repositorio Institucional EdocURLeishmania donovani chagasiLutzomyia longipalpisSolanum quitoenseCell morphometryPercentage of infectionPromastigoteTemperature and co 2Ultrastructure characteristicsInteraction of leishmania (L.) chagasi with the lulo cell line in different environmental conditionsInteracción de leishmania (L.) chagasi con la línea celular lulo en diferentes condiciones ambientalesarticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Acero V.Galeano E.Ayala M.Castellanos J.Bello F.ORIGINALv32n2a10.pdfapplication/pdf560990https://repository.urosario.edu.co/bitstreams/a4eaf40f-a8d5-4f9b-9656-2e1431b07de6/downloadaf0fdfac54ed991e5f995d8a05f5a34dMD51TEXTv32n2a10.pdf.txtv32n2a10.pdf.txtExtracted texttext/plain45496https://repository.urosario.edu.co/bitstreams/43323365-a153-40be-8801-07acebd4481d/download8d4420caac029f7345b5b45a1944b3ddMD52THUMBNAILv32n2a10.pdf.jpgv32n2a10.pdf.jpgGenerated Thumbnailimage/jpeg4561https://repository.urosario.edu.co/bitstreams/d2302742-ee63-4474-b102-22b343adaabf/download49bf9a354dc4140c30cfb2a1d4af8becMD5310336/23007oai:repository.urosario.edu.co:10336/230072022-05-02 07:37:14.438998https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co |
dc.title.spa.fl_str_mv |
Interaction of leishmania (L.) chagasi with the lulo cell line in different environmental conditions |
dc.title.TranslatedTitle.spa.fl_str_mv |
Interacción de leishmania (L.) chagasi con la línea celular lulo en diferentes condiciones ambientales |
title |
Interaction of leishmania (L.) chagasi with the lulo cell line in different environmental conditions |
spellingShingle |
Interaction of leishmania (L.) chagasi with the lulo cell line in different environmental conditions Leishmania donovani chagasi Lutzomyia longipalpis Solanum quitoense Cell morphometry Percentage of infection Promastigote Temperature and co 2 Ultrastructure characteristics |
title_short |
Interaction of leishmania (L.) chagasi with the lulo cell line in different environmental conditions |
title_full |
Interaction of leishmania (L.) chagasi with the lulo cell line in different environmental conditions |
title_fullStr |
Interaction of leishmania (L.) chagasi with the lulo cell line in different environmental conditions |
title_full_unstemmed |
Interaction of leishmania (L.) chagasi with the lulo cell line in different environmental conditions |
title_sort |
Interaction of leishmania (L.) chagasi with the lulo cell line in different environmental conditions |
dc.subject.keyword.spa.fl_str_mv |
Leishmania donovani chagasi Lutzomyia longipalpis Solanum quitoense Cell morphometry Percentage of infection Promastigote Temperature and co 2 Ultrastructure characteristics |
topic |
Leishmania donovani chagasi Lutzomyia longipalpis Solanum quitoense Cell morphometry Percentage of infection Promastigote Temperature and co 2 Ultrastructure characteristics |
description |
There are unknown or poorly understood events in the Leishmania-host cell interactions that merit studies in new in vitro models. The aim of the present work was to evaluate Leishmania chagasi interaction with a cell line (Lulo) previously established and derived from Lutzomyia longipalpis embryonic tissues, bearing different temperatures and CO 2 concentrations in mind and morphometric changes of the cells. The J774 cell line was used as positive control of the infection. The L. chagasi MH/CO/84/CL-044B strain was used for infection assays. The parasites were added to adherent cells, in Lulo as well as J774, in a 5:1 ratio. Incubation was at 28°C and 37°C, in the absence and presence of CO 2. A morphometric study of the cells was carried out before and after infection. Both parasite and cell ultrastructure characteristics were analyzed by electron microscope. The greatest percentage of infection in the Lulo cells at 28°C (26,8%) was registered on day six post infection, while at 37°C, in the absence of CO 2, the maximum value (30,4%) was obtained on day nine. The morphometric analyses showed a significant increase in the size of Lulo cells infected at 28°C and J774 cells at 37°C with and without CO 2, respectively. |
publishDate |
2006 |
dc.date.created.spa.fl_str_mv |
2006 |
dc.date.accessioned.none.fl_str_mv |
2020-05-25T23:59:13Z |
dc.date.available.none.fl_str_mv |
2020-05-25T23:59:13Z |
dc.type.eng.fl_str_mv |
article |
dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
dc.type.spa.spa.fl_str_mv |
Artículo |
dc.identifier.issn.none.fl_str_mv |
1200488 |
dc.identifier.uri.none.fl_str_mv |
https://repository.urosario.edu.co/handle/10336/23007 |
identifier_str_mv |
1200488 |
url |
https://repository.urosario.edu.co/handle/10336/23007 |
dc.language.iso.spa.fl_str_mv |
spa |
language |
spa |
dc.relation.citationEndPage.none.fl_str_mv |
171 |
dc.relation.citationIssue.none.fl_str_mv |
No. 2 |
dc.relation.citationStartPage.none.fl_str_mv |
165 |
dc.relation.citationTitle.none.fl_str_mv |
Revista Colombiana de Entomologia |
dc.relation.citationVolume.none.fl_str_mv |
Vol. 32 |
dc.relation.ispartof.spa.fl_str_mv |
Revista Colombiana de Entomologia, ISSN:1200488, Vol.32, No.2 (2006); pp. 165-171 |
dc.relation.uri.spa.fl_str_mv |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-79960469168&partnerID=40&md5=3c70dbc529752606952e463511204368 |
dc.rights.coar.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
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Abierto (Texto Completo) |
rights_invalid_str_mv |
Abierto (Texto Completo) http://purl.org/coar/access_right/c_abf2 |
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application/pdf |
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Universidad del Rosario |
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reponame:Repositorio Institucional EdocUR |
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