Validation of real-time PCR assays for detecting Plasmodium and Babesia DNA species in blood samples
Malaria and babesiosis are global health threats affecting humans, wildlife, and domestic animals, particularly in Africa, the Americas, and Europe. Malaria can lead to severe outcomes, while babesiosis usually resembles a mild illness but can be severe and fatal in individuals with weakened immune...
- Autores:
- Tipo de recurso:
- Fecha de publicación:
- 2024
- Institución:
- Universidad del Rosario
- Repositorio:
- Repositorio EdocUR - U. Rosario
- Idioma:
- eng
- OAI Identifier:
- oai:repository.urosario.edu.co:10336/44850
- Acceso en línea:
- https://doi.org/10.1016/j.actatropica.2024.107350
https://repository.urosario.edu.co/handle/10336/44850
- Palabra clave:
- Real-time
PCRP
Lasmodium
Babesia
Sensitivity
- Rights
- License
- Attribution-NonCommercial-NoDerivatives 4.0 International
id |
EDOCUR2_0749365a8fa4556588942ae7d20f8c8c |
---|---|
oai_identifier_str |
oai:repository.urosario.edu.co:10336/44850 |
network_acronym_str |
EDOCUR2 |
network_name_str |
Repositorio EdocUR - U. Rosario |
repository_id_str |
|
spelling |
dc30c120-c40b-4f9c-92f2-245f57c6dcde797e15ee-3f51-49c8-9f1b-b1e61db4a9307fd8b73d-5864-44a7-b0f8-4a8d491a557f6f44215e-9571-4791-bfe9-918e2a499ce9d47af648-5f78-486c-893d-4b861642d4eb48d14312-00fa-4ed2-ad54-6aa14ae1a58d10117161186002025-01-26T18:37:36Z2025-01-26T18:37:36Z2024-10-012024-10-01Malaria and babesiosis are global health threats affecting humans, wildlife, and domestic animals, particularly in Africa, the Americas, and Europe. Malaria can lead to severe outcomes, while babesiosis usually resembles a mild illness but can be severe and fatal in individuals with weakened immune systems. Swift, accurate detection of these parasites is crucial for treatment and control. We evaluated a real-time PCR assay for diagnosing five Plasmodium and three Babesia species from blood samples, assessing its sensitivity, specificity, and analytical performance by analyzing 46 malaria-positive and 32 Babesia spp-positive samples diagnosed through microscopy. The limit of detection for Plasmodium species ranged from 30 to 0.0003 copies/µL. For mixed infections, it was 0.3 copies/µL for P. falciparum/P. vivax and 3 copies/µL for P. malariae/P. knowlesi. Babesia species had a detection limit of 0.2 copies/µL. No cross-reactivity was observed among 64 DNA samples from various microorganisms. The assay showed good sensitivity, detecting Plasmodium and Babesia species with 100 % accuracy overall, except for P. falciparum (97.7 %) and B. microti (12.5 %). The low sensitivity of detecting B. microti was attributed to limitations in microscopy for species identification. This technique heavily relies on the proficiency of the examiner, as species within the genus cannot be distinguished under a microscope. Additionally, Babesia can be confused with the early trophozoite stage (ring forms) of Plasmodium parasites. The findings support multiplex qPCR's diagnostic superiority over the gold standard, despite higher costs. It offers enhanced sensitivity, specificity, and detects mixed infections, crucial for effective monitoring and diagnosis of malaria and babesiosis in endemic regions with significant public health challenges.application/pdfhttps://doi.org/10.1016/j.actatropica.2024.107350https://repository.urosario.edu.co/handle/10336/44850engActa TropicaActa TropicaAttribution-NonCommercial-NoDerivatives 4.0 InternationalAbierto (Texto Completo)http://creativecommons.org/licenses/by-nc-sa/4.0/http://purl.org/coar/access_right/c_abf2Acta Tropicainstname:Universidad del Rosarioreponame:Repositorio Institucional EdocURReal-timePCRPLasmodiumBabesiaSensitivityValidation of real-time PCR assays for detecting Plasmodium and Babesia DNA species in blood samplesarticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Patiño, LHCastañeda, SCamargo, MCao, LYLiggayu, BPaniz-Mondolfi, ARamírez González, Juan DavidORIGINALValidation_of_real-time_PCR.pdfapplication/pdf4489864https://repository.urosario.edu.co/bitstreams/ae55e83f-72da-4b58-a5c6-51940f09c8d0/download748f188f0b38eeca18724d979efed561MD51TEXTValidation_of_real-time_PCR.pdf.txtValidation_of_real-time_PCR.pdf.txtExtracted texttext/plain99732https://repository.urosario.edu.co/bitstreams/196771e7-5207-413b-b67d-45ae28ec48e8/downloada9079ef3ff71b28ffcde8bd7613353b9MD52THUMBNAILValidation_of_real-time_PCR.pdf.jpgValidation_of_real-time_PCR.pdf.jpgGenerated Thumbnailimage/jpeg4514https://repository.urosario.edu.co/bitstreams/864affd1-eaea-4cef-843e-a9696a38309a/downloadd8b25309577a1e9ecb1611b538b5f3caMD5310336/44850oai:repository.urosario.edu.co:10336/448502025-01-27 03:01:17.301http://creativecommons.org/licenses/by-nc-sa/4.0/Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttps://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co |
dc.title.spa.fl_str_mv |
Validation of real-time PCR assays for detecting Plasmodium and Babesia DNA species in blood samples |
title |
Validation of real-time PCR assays for detecting Plasmodium and Babesia DNA species in blood samples |
spellingShingle |
Validation of real-time PCR assays for detecting Plasmodium and Babesia DNA species in blood samples Real-time PCRP Lasmodium Babesia Sensitivity |
title_short |
Validation of real-time PCR assays for detecting Plasmodium and Babesia DNA species in blood samples |
title_full |
Validation of real-time PCR assays for detecting Plasmodium and Babesia DNA species in blood samples |
title_fullStr |
Validation of real-time PCR assays for detecting Plasmodium and Babesia DNA species in blood samples |
title_full_unstemmed |
Validation of real-time PCR assays for detecting Plasmodium and Babesia DNA species in blood samples |
title_sort |
Validation of real-time PCR assays for detecting Plasmodium and Babesia DNA species in blood samples |
dc.subject.spa.fl_str_mv |
Real-time PCRP Lasmodium Babesia Sensitivity |
topic |
Real-time PCRP Lasmodium Babesia Sensitivity |
description |
Malaria and babesiosis are global health threats affecting humans, wildlife, and domestic animals, particularly in Africa, the Americas, and Europe. Malaria can lead to severe outcomes, while babesiosis usually resembles a mild illness but can be severe and fatal in individuals with weakened immune systems. Swift, accurate detection of these parasites is crucial for treatment and control. We evaluated a real-time PCR assay for diagnosing five Plasmodium and three Babesia species from blood samples, assessing its sensitivity, specificity, and analytical performance by analyzing 46 malaria-positive and 32 Babesia spp-positive samples diagnosed through microscopy. The limit of detection for Plasmodium species ranged from 30 to 0.0003 copies/µL. For mixed infections, it was 0.3 copies/µL for P. falciparum/P. vivax and 3 copies/µL for P. malariae/P. knowlesi. Babesia species had a detection limit of 0.2 copies/µL. No cross-reactivity was observed among 64 DNA samples from various microorganisms. The assay showed good sensitivity, detecting Plasmodium and Babesia species with 100 % accuracy overall, except for P. falciparum (97.7 %) and B. microti (12.5 %). The low sensitivity of detecting B. microti was attributed to limitations in microscopy for species identification. This technique heavily relies on the proficiency of the examiner, as species within the genus cannot be distinguished under a microscope. Additionally, Babesia can be confused with the early trophozoite stage (ring forms) of Plasmodium parasites. The findings support multiplex qPCR's diagnostic superiority over the gold standard, despite higher costs. It offers enhanced sensitivity, specificity, and detects mixed infections, crucial for effective monitoring and diagnosis of malaria and babesiosis in endemic regions with significant public health challenges. |
publishDate |
2024 |
dc.date.created.spa.fl_str_mv |
2024-10-01 |
dc.date.issued.spa.fl_str_mv |
2024-10-01 |
dc.date.accessioned.none.fl_str_mv |
2025-01-26T18:37:36Z |
dc.date.available.none.fl_str_mv |
2025-01-26T18:37:36Z |
dc.type.spa.fl_str_mv |
article |
dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
dc.type.spa.spa.fl_str_mv |
Artículo |
dc.identifier.doi.spa.fl_str_mv |
https://doi.org/10.1016/j.actatropica.2024.107350 |
dc.identifier.uri.none.fl_str_mv |
https://repository.urosario.edu.co/handle/10336/44850 |
url |
https://doi.org/10.1016/j.actatropica.2024.107350 https://repository.urosario.edu.co/handle/10336/44850 |
dc.language.iso.spa.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.spa.fl_str_mv |
Acta Tropica |
dc.rights.spa.fl_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International |
dc.rights.coar.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
dc.rights.acceso.spa.fl_str_mv |
Abierto (Texto Completo) |
dc.rights.uri.spa.fl_str_mv |
http://creativecommons.org/licenses/by-nc-sa/4.0/ |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International Abierto (Texto Completo) http://creativecommons.org/licenses/by-nc-sa/4.0/ http://purl.org/coar/access_right/c_abf2 |
dc.format.mimetype.spa.fl_str_mv |
application/pdf |
dc.publisher.spa.fl_str_mv |
Acta Tropica |
dc.source.spa.fl_str_mv |
Acta Tropica |
institution |
Universidad del Rosario |
dc.source.instname.spa.fl_str_mv |
instname:Universidad del Rosario |
dc.source.reponame.spa.fl_str_mv |
reponame:Repositorio Institucional EdocUR |
bitstream.url.fl_str_mv |
https://repository.urosario.edu.co/bitstreams/ae55e83f-72da-4b58-a5c6-51940f09c8d0/download https://repository.urosario.edu.co/bitstreams/196771e7-5207-413b-b67d-45ae28ec48e8/download https://repository.urosario.edu.co/bitstreams/864affd1-eaea-4cef-843e-a9696a38309a/download |
bitstream.checksum.fl_str_mv |
748f188f0b38eeca18724d979efed561 a9079ef3ff71b28ffcde8bd7613353b9 d8b25309577a1e9ecb1611b538b5f3ca |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositorio institucional EdocUR |
repository.mail.fl_str_mv |
edocur@urosario.edu.co |
_version_ |
1828160745215361024 |