Identification, characterization and antigenicity of the Plasmodium vivax rhoptry neck protein 1 (PvRON1)

Background: Plasmodium vivax malaria remains a major health problem in tropical and sub-tropical regions worldwide. Several rhoptry proteins which are important for interaction with and/or invasion of red blood cells, such as PfRONs, Pf92, Pf38, Pf12 and Pf34, have been described during the last few...

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Fecha de publicación:
2011
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
eng
OAI Identifier:
oai:repository.urosario.edu.co:10336/8762
Acceso en línea:
http://repository.urosario.edu.co/handle/10336/8762
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Enfermedades
Plasmodium vivax rhoptry neck protein 1 (PvRON1)
Toxoplasma
Plasmodium
Proteinas
Inmunología
RED-BLOOD-CELLS
TOXOPLASMA-GONDII
MALARIA PARASITES
CIRCUMSPOROZOITE PROTEIN
FALCIPARUM MEROZOITES
MONOCLONAL-ANTIBODIES
COMPARATIVE GENOMICS
SURFACE PROTEIN
GLYCOSYLPHOSPHATIDYLINOSITOL
PREDICTION
Rights
License
Abierto (Texto completo)
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repository_id_str
dc.title.spa.fl_str_mv Identification, characterization and antigenicity of the Plasmodium vivax rhoptry neck protein 1 (PvRON1)
title Identification, characterization and antigenicity of the Plasmodium vivax rhoptry neck protein 1 (PvRON1)
spellingShingle Identification, characterization and antigenicity of the Plasmodium vivax rhoptry neck protein 1 (PvRON1)
Enfermedades
Plasmodium vivax rhoptry neck protein 1 (PvRON1)
Toxoplasma
Plasmodium
Proteinas
Inmunología
RED-BLOOD-CELLS
TOXOPLASMA-GONDII
MALARIA PARASITES
CIRCUMSPOROZOITE PROTEIN
FALCIPARUM MEROZOITES
MONOCLONAL-ANTIBODIES
COMPARATIVE GENOMICS
SURFACE PROTEIN
GLYCOSYLPHOSPHATIDYLINOSITOL
PREDICTION
title_short Identification, characterization and antigenicity of the Plasmodium vivax rhoptry neck protein 1 (PvRON1)
title_full Identification, characterization and antigenicity of the Plasmodium vivax rhoptry neck protein 1 (PvRON1)
title_fullStr Identification, characterization and antigenicity of the Plasmodium vivax rhoptry neck protein 1 (PvRON1)
title_full_unstemmed Identification, characterization and antigenicity of the Plasmodium vivax rhoptry neck protein 1 (PvRON1)
title_sort Identification, characterization and antigenicity of the Plasmodium vivax rhoptry neck protein 1 (PvRON1)
dc.subject.ddc.none.fl_str_mv Enfermedades
topic Enfermedades
Plasmodium vivax rhoptry neck protein 1 (PvRON1)
Toxoplasma
Plasmodium
Proteinas
Inmunología
RED-BLOOD-CELLS
TOXOPLASMA-GONDII
MALARIA PARASITES
CIRCUMSPOROZOITE PROTEIN
FALCIPARUM MEROZOITES
MONOCLONAL-ANTIBODIES
COMPARATIVE GENOMICS
SURFACE PROTEIN
GLYCOSYLPHOSPHATIDYLINOSITOL
PREDICTION
dc.subject.decs.spa.fl_str_mv Plasmodium vivax rhoptry neck protein 1 (PvRON1)
Toxoplasma
Plasmodium
Proteinas
Inmunología
dc.subject.keyword.eng.fl_str_mv RED-BLOOD-CELLS
TOXOPLASMA-GONDII
MALARIA PARASITES
CIRCUMSPOROZOITE PROTEIN
FALCIPARUM MEROZOITES
MONOCLONAL-ANTIBODIES
COMPARATIVE GENOMICS
SURFACE PROTEIN
GLYCOSYLPHOSPHATIDYLINOSITOL
PREDICTION
description Background: Plasmodium vivax malaria remains a major health problem in tropical and sub-tropical regions worldwide. Several rhoptry proteins which are important for interaction with and/or invasion of red blood cells, such as PfRONs, Pf92, Pf38, Pf12 and Pf34, have been described during the last few years and are being considered as potential anti-malarial vaccine candidates. This study describes the identification and characterization of the P. vivax rhoptry neck protein 1 (PvRON1) and examine its antigenicity in natural P. vivax infections. Methods: The PvRON1 encoding gene, which is homologous to that encoding the P. falciparum apical sushi protein (ASP) according to the plasmoDB database, was selected as our study target. The pvron1 gene transcription was evaluated by RT-PCR using RNA obtained from the P. vivax VCG-1 strain. Two peptides derived from the deduced P. vivax Sal-I PvRON1 sequence were synthesized and inoculated in rabbits for obtaining anti-PvRON1 antibodies which were used to confirm the protein expression in VCG-1 strain schizonts along with its association with detergent-resistant microdomains (DRMs) by Western blot, and its localization by immunofluorescence assays. The antigenicity of the PvRON1 protein was assessed using human sera from individuals previously exposed to P. vivax malaria by ELISA. Results: In the P. vivax VCG-1 strain, RON1 is a 764 amino acid-long protein. In silico analysis has revealed that PvRON1 shares essential characteristics with different antigens involved in invasion, such as the presence of a secretory signal, a GPI-anchor sequence and a putative sushi domain. The PvRON1 protein is expressed in parasite's schizont stage, localized in rhoptry necks and it is associated with DRMs. Recombinant protein recognition by human sera indicates that this antigen can trigger an immune response during a natural infection with P. vivax. Conclusions: This study shows the identification and characterization of the P. vivax rhoptry neck protein 1 in the VCG-1 strain. Taking into account that PvRON1 shares several important characteristics with other Plasmodium antigens that play a functional role during RBC invasion and, as shown here, it is antigenic, it could be considered as a good vaccine candidate. Further studies aimed at assessing its immunogenicity and protection-inducing ability in the Aotus monkey model are thus recommended.
publishDate 2011
dc.date.created.none.fl_str_mv 2011-10-24
dc.date.issued.none.fl_str_mv 2011
dc.date.accessioned.none.fl_str_mv 2014-08-11T14:09:59Z
dc.date.available.none.fl_str_mv 2014-08-11T14:09:59Z
dc.type.eng.fl_str_mv article
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.spa.spa.fl_str_mv Artículo
dc.identifier.issn.none.fl_str_mv ISSN:1475-2875
dc.identifier.uri.none.fl_str_mv http://repository.urosario.edu.co/handle/10336/8762
identifier_str_mv ISSN:1475-2875
url http://repository.urosario.edu.co/handle/10336/8762
dc.language.iso.none.fl_str_mv eng
language eng
dc.relation.citationIssue.none.fl_str_mv No. 314
dc.relation.citationTitle.none.fl_str_mv MALARIA JOURNAL
dc.relation.citationVolume.none.fl_str_mv Vol. 10
dc.relation.ispartof.spa.fl_str_mv MALARIA JOURNAL ISSN: 1475-2875 V. 10 N. 314 Oct 24, 2011
dc.relation.uri.none.fl_str_mv http://www.malariajournal.com/content/10/1/314
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.acceso.spa.fl_str_mv Abierto (Texto completo)
rights_invalid_str_mv Abierto (Texto completo)
http://purl.org/coar/access_right/c_abf2
dc.format.medium.spa.fl_str_mv Recurso electrónico
dc.format.mimetype.none.fl_str_mv application/pdf
dc.format.tipo.spa.fl_str_mv Documento
dc.publisher.spa.fl_str_mv Universidad del Rosario
institution Universidad del Rosario
dc.source.instname.spa.fl_str_mv instname:Universidad del Rosario
dc.source.reponame.spa.fl_str_mv reponame:Repositorio Institucional EdocUR
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spelling Comunidad Rosarista4138d92d-a7e8-46a5-b415-2d76a4bb9f23600c3238f3e-9794-411a-a685-980631352d0660010ecd4f9-843f-4ef2-bec0-7d39d3381a13600796530656002014-08-11T14:09:59Z2014-08-11T14:09:59Z2011-10-242011Background: Plasmodium vivax malaria remains a major health problem in tropical and sub-tropical regions worldwide. Several rhoptry proteins which are important for interaction with and/or invasion of red blood cells, such as PfRONs, Pf92, Pf38, Pf12 and Pf34, have been described during the last few years and are being considered as potential anti-malarial vaccine candidates. This study describes the identification and characterization of the P. vivax rhoptry neck protein 1 (PvRON1) and examine its antigenicity in natural P. vivax infections. Methods: The PvRON1 encoding gene, which is homologous to that encoding the P. falciparum apical sushi protein (ASP) according to the plasmoDB database, was selected as our study target. The pvron1 gene transcription was evaluated by RT-PCR using RNA obtained from the P. vivax VCG-1 strain. Two peptides derived from the deduced P. vivax Sal-I PvRON1 sequence were synthesized and inoculated in rabbits for obtaining anti-PvRON1 antibodies which were used to confirm the protein expression in VCG-1 strain schizonts along with its association with detergent-resistant microdomains (DRMs) by Western blot, and its localization by immunofluorescence assays. The antigenicity of the PvRON1 protein was assessed using human sera from individuals previously exposed to P. vivax malaria by ELISA. Results: In the P. vivax VCG-1 strain, RON1 is a 764 amino acid-long protein. In silico analysis has revealed that PvRON1 shares essential characteristics with different antigens involved in invasion, such as the presence of a secretory signal, a GPI-anchor sequence and a putative sushi domain. The PvRON1 protein is expressed in parasite's schizont stage, localized in rhoptry necks and it is associated with DRMs. Recombinant protein recognition by human sera indicates that this antigen can trigger an immune response during a natural infection with P. vivax. Conclusions: This study shows the identification and characterization of the P. vivax rhoptry neck protein 1 in the VCG-1 strain. Taking into account that PvRON1 shares several important characteristics with other Plasmodium antigens that play a functional role during RBC invasion and, as shown here, it is antigenic, it could be considered as a good vaccine candidate. Further studies aimed at assessing its immunogenicity and protection-inducing ability in the Aotus monkey model are thus recommended.Recurso electrónicoapplication/pdfDocumentoISSN:1475-2875http://repository.urosario.edu.co/handle/10336/8762engUniversidad del RosarioNo. 314MALARIA JOURNALVol. 10MALARIA JOURNAL ISSN: 1475-2875 V. 10 N. 314 Oct 24, 2011http://www.malariajournal.com/content/10/1/314Abierto (Texto completo)EL AUTOR, manifiesta que la obra objeto de la presente autorización es original y la realizó sin violar o usurpar derechos de autor de terceros, por lo tanto la obra es de exclusiva autoría y tiene la titularidad sobre la misma.http://purl.org/coar/access_right/c_abf2instname:Universidad del Rosarioreponame:Repositorio Institucional EdocUREnfermedades616600Plasmodium vivax rhoptry neck protein 1 (PvRON1)ToxoplasmaPlasmodiumProteinasInmunologíaRED-BLOOD-CELLSTOXOPLASMA-GONDIIMALARIA PARASITESCIRCUMSPOROZOITE PROTEINFALCIPARUM MEROZOITESMONOCLONAL-ANTIBODIESCOMPARATIVE GENOMICSSURFACE PROTEINGLYCOSYLPHOSPHATIDYLINOSITOLPREDICTIONIdentification, characterization and antigenicity of the Plasmodium vivax rhoptry neck protein 1 (PvRON1)articleArtículohttp://purl.org/coar/resource_type/c_6501Moreno Pérez, Darwin AMontenegro, MarjoriePatarroyo, Manuel E.Patarroyo, Manuel A.Moreno Perez, Darwin AMontenegro, MarjoriePatarroyo, Manuel E.Patarroyo, Manuel A.ORIGINALIdentification, characterization and antigenicity.pdfIdentification, characterization and antigenicity.pdfapplication/pdf2560084https://repository.urosario.edu.co/bitstreams/e50c2a1f-8bdc-47fd-a5a6-9b8fd3788e80/downloadb2803336e90f40397f6aec7a5fcc1e07MD51LICENSElicense.txtlicense.txttext/plain2156https://repository.urosario.edu.co/bitstreams/d01f1aa7-6472-4c0d-b5b6-d320039830f2/downloadb4f8fe66e94b897ab4c355bac005ad16MD52TEXTIdentification, characterization and antigenicity.pdf.txtIdentification, characterization and antigenicity.pdf.txtExtracted 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