Extraction and partial characterization of venom from the Colombian spider Pamphobeteus aff. nigricolor (Aranae:Theraphosidae)

We report the first studies of characterization and extraction of the Pamphobeteus aff. nigricolor (Pocock, 1901) (Aranae:Theraphosidae) venom done in Colombia using the electro-stimulation technique previous anesthesia with isofluorane. After each extraction process, a low viscosity, colorless venom was obtained. This venom showed a 1.01 mg/µl density and a pH of 5. The humidity percentage did not show a significance difference between males and females (P > 0.05) with a general media of 77.49 ± 1.74%. In all cases the venom yielded was variable between males and females, with a media of 22.45 ± 5.17 mg (wet weight) and 4.58 ± 0.94 mg (dry weigh), obtaining larger amounts in females, 28.34 ± 7.49 mg and 5.69 ± 1.36 (wet and dry weight respectively). Venom showed a hemolytic activity dependent of enzymatic active phospholipase and neither coagulant nor proteolytic activities were observed. Electrophoretic profile showed a main protein content with a molecular mass below 14 kDa. RP-HPLC venom profile revealed a difference among male and female venom's content where 17 and 21 main fractions were obtained respectively. Three peptides, Theraphotoxin-Pn1a, Theraphotoxin-Pn1b and Theraphotoxin-Pn2a, were identified using HPLC-nESI-MS/MS. These peptides showed a high identity with other peptides found on Theraphosides which are proved to affect voltage-gated calcium channels. © 2013 Elsevier Ltd. All rights reserved.

• Autores: Estrada-Gomez S.; Vargas Muñoz, Leidy Johana; Quintana Castillo, Juan Carlos
• Tipo de recurso: Article of journal
• Fecha de publicación: 2013
• Institución: Universidad Cooperativa de Colombia
• Repositorio: Repositorio UCC
• OAI Identifier: oai:repository.ucc.edu.co:20.500.12494/41609
• Acceso en línea: https://doi.org/10.1155/2017/1090534; https://hemeroteca.unad.edu.co/index.php/riaa/article/view/2369/2665; https://hdl.handle.net/20.500.12494/41609
• Palabra clave: phospholipase A2; spider venom; theraphotoxin Pn1a; theraphotoxin Pn1b; theraphotoxin Pn2a; toxin; unclassified drug; voltage gated calcium channel; animal tissue; article; blood clotting; Colombia; dry weight; electrophoresis; electrospray mass spectrometry; female; hemolysis; high performance liquid chromatography; humidity; male; nonhuman; Pamphobeteus nigricolor; peptide analysis; pH measurement; priority journal; protein degradation; reversed phase liquid chromatography; tarantula; toxin analysis; viscosity; Araneae; Theraphosidae; Pamphobeteus nigricolor; RP-HPLC; Theraphosidae; Venom extraction; Animals; Chemical Fractionation; Chromatography; High Pressure Liquid; Chromatography; Reverse-Phase; Electrophoresis; Polyacrylamide Gel; Female; Male; Sex Factors; Spider Venoms; Spiders
• Rights: closedAccess
• License: http://purl.org/coar/access_right/c_14cb