Evaluación del potencial antimicrobiano del extracto de cáscara, semilla y pulpa de la Vitis vinífera sobre el cultivo de S. mutans
ABSTRACT Introduction: In the ongoing search for novel natural agents with potential health benefits, phenolic compounds have garnered significant attention due to their demonstrated antimicrobial and anti-inflammatory properties. Among these compounds, resveratrol and flavonoids, which are abundant...
- Autores:
-
Montoya Osorio, Daniela
Barrera Pino, Ana María
Caballero Montoya, Daniela
Peláez Vargas, Alejandro
- Tipo de recurso:
- Trabajo de grado de pregrado
- Fecha de publicación:
- 2024
- Institución:
- Universidad Cooperativa de Colombia
- Repositorio:
- Repositorio UCC
- Idioma:
- spa
- OAI Identifier:
- oai:repository.ucc.edu.co:20.500.12494/55914
- Acceso en línea:
- https://hdl.handle.net/20.500.12494/55914
- Palabra clave:
- Extractos
Vitis Vinefera
S. mutans
Clorhexidina
Extracts
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by-nc-nd/4.0/
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Evaluación del potencial antimicrobiano del extracto de cáscara, semilla y pulpa de la Vitis vinífera sobre el cultivo de S. mutans |
| title |
Evaluación del potencial antimicrobiano del extracto de cáscara, semilla y pulpa de la Vitis vinífera sobre el cultivo de S. mutans |
| spellingShingle |
Evaluación del potencial antimicrobiano del extracto de cáscara, semilla y pulpa de la Vitis vinífera sobre el cultivo de S. mutans Extractos Vitis Vinefera S. mutans Clorhexidina Extracts |
| title_short |
Evaluación del potencial antimicrobiano del extracto de cáscara, semilla y pulpa de la Vitis vinífera sobre el cultivo de S. mutans |
| title_full |
Evaluación del potencial antimicrobiano del extracto de cáscara, semilla y pulpa de la Vitis vinífera sobre el cultivo de S. mutans |
| title_fullStr |
Evaluación del potencial antimicrobiano del extracto de cáscara, semilla y pulpa de la Vitis vinífera sobre el cultivo de S. mutans |
| title_full_unstemmed |
Evaluación del potencial antimicrobiano del extracto de cáscara, semilla y pulpa de la Vitis vinífera sobre el cultivo de S. mutans |
| title_sort |
Evaluación del potencial antimicrobiano del extracto de cáscara, semilla y pulpa de la Vitis vinífera sobre el cultivo de S. mutans |
| dc.creator.fl_str_mv |
Montoya Osorio, Daniela Barrera Pino, Ana María Caballero Montoya, Daniela Peláez Vargas, Alejandro |
| dc.contributor.advisor.none.fl_str_mv |
Peláez Vargas, Alejandro |
| dc.contributor.author.none.fl_str_mv |
Montoya Osorio, Daniela Barrera Pino, Ana María Caballero Montoya, Daniela Peláez Vargas, Alejandro |
| dc.contributor.researchgroup.none.fl_str_mv |
GIOM |
| dc.subject.proposal.spa.fl_str_mv |
Extractos Vitis Vinefera S. mutans Clorhexidina |
| topic |
Extractos Vitis Vinefera S. mutans Clorhexidina Extracts |
| dc.subject.proposal.none.fl_str_mv |
Extracts |
| description |
ABSTRACT Introduction: In the ongoing search for novel natural agents with potential health benefits, phenolic compounds have garnered significant attention due to their demonstrated antimicrobial and anti-inflammatory properties. Among these compounds, resveratrol and flavonoids, which are abundant in various plant sources, have shown promise in combating microbial infections and reducing inflammation. Materials and Methods: Three types of extracts were evaluated: One commercial and two experimental (aqueous and hydroalcoholic), prepared from the peel, seed, and pulp of Vitis vinifera. Two different dehydration temperatures were used. The S. mutans strain was selected and cultured on BHI agar plates, which were then treated with the extracts. Inhibition zones were measured at various times, with 0.2% chlorhexidine used as a positive control and sterile saline solution as a negative control. Results. No inhibition zones were found for any of the commercial extracts or experimental. Only inhibition zones were observed in the positive control of Chlorhexidine. Conclusions. The commercial extract and the hydroalcoholic and aqueous experimental extracts prepared from Vitis vinifera grape did not exhibit inhibition against S. mutans. |
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2024 |
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2024-06-12T18:22:17Z |
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2024-06-12T18:22:17Z 2028-06-11 |
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2024-06-11 |
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Trabajo de grado - Pregrado |
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Montoya Osorio, D.M., Barrera Pino, A.M., Caballero Montoya, D. y Peláez Vargas A. (2024). Evaluación del potencial antimicrobiano del extracto de cáscara, semilla y pulpa de la Vitis vinífera sobre el cultivo de S. mutans. [Tesis de pregrado, Universidad Cooperativa de Colombia]. Repositorio Institucional Universidad Cooperativa de Colombia.https://hdl.handle.net/20.500.12494/55914 |
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https://hdl.handle.net/20.500.12494/55914 |
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Montoya Osorio, D.M., Barrera Pino, A.M., Caballero Montoya, D. y Peláez Vargas A. (2024). Evaluación del potencial antimicrobiano del extracto de cáscara, semilla y pulpa de la Vitis vinífera sobre el cultivo de S. mutans. [Tesis de pregrado, Universidad Cooperativa de Colombia]. Repositorio Institucional Universidad Cooperativa de Colombia.https://hdl.handle.net/20.500.12494/55914 |
| url |
https://hdl.handle.net/20.500.12494/55914 |
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spa |
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spa |
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Molina-Quijada DMA, Medina-Juárez LA, González-Aguilar GA, Robles-Sánchez RM, Gámez-Meza N. Compuestos fenólicos y actividad antioxidante de cáscara de uva (Vitis vinifera L.) de mesa cultivada en el noroeste de México Phenolic compounds and antioxidant activity of table grape (Vitis vinifera L.) skin from northwest Mexico. CYTA - J Food. 2010;8(1):57–63. Nardini M. Compounds in Food: Characterization and Health Benefits. Molecules. 2022;25(27(3)):783. Wu CD. Grape products and oral health. J Nutr. 2009;139(9):3–8. Surco-laos F, Paucar HA, Gamboa WQ. Determinación De Polifenoles Totales Y Actividad Phenolic Compounds and Antioxidant Activity of Grape Seeds Residues of Pisco Production. Rev Soc Quim Perú. 2020;86(2):123–31. Clarke TK. Streptococcus mutans and dental caries. Br Med J. 1975;4(5997):647–8. Schindelin J, Arganda-Carrera I, Frise E, Verena K, Mark L, Tobias P, et al. Fiji - an Open platform for biological image analysis. Nat Methods. 2009;9(7). Aleixandre-Tudo JL, Buica A, Nieuwoudt H, Aleixandre JL, Du Toit W. Spectrophotometric Analysis of Phenolic Compounds in Grapes and Wines. J Agric Food Chem. 2017;65(20):4009–26. Reyes D, Ortega D, Quintero J, Piquer S, Alarcón M, Silva RF Da. Efecto antimicrobiano del extracto foliar de mango (Mangifera indica L. cv. Bocado) en microorganismos de interés clínico. Rev Salus [Internet]. 2017;21(2):7–13. Navarrete Barragán NA, Pita-Ospina EF, Sánchez Mora. RM, Giraldo Quintero SE, Bernal Lizarazú MC. Actividad in vitro de los extractos etanólicos de Lantana camara L., Petiveria alliacea L. y Lippia dulcis T. frente a bacterias patógenas. Nova. 2020;18(33):53–71. Castellanos JS, Betancourt DE, Báez DD, Baldión PA. Effect of flavonoids from grape seed and cranberry extracts on the microbiological activity of Streptococcus mutans : a systematic review of in vitro studies. BMC Oral Health. 2024;1–21. Estrada-Reyes R, Araujo-Escalona AG. Los flavonoides y el Sistema Nervioso Central Rosa. Salud Ment [Internet]. 2012;35(5):375–84. Sarduy-Bermúdez L, González-Díaz ME. La biopelícula: una nueva concepción de la placa dentobacteriana. Medicent Electrón. 2016;20(3):167–75. Cruz Quintana SM, Díaz Sjostrom P, Arias Socarrás D, Mazón Baldeón GM. Microbiota of oral cavity ecosystems. Rev Cubana Estomatol. 2017;54(1):84–99. Sandoval M, Lazarte K, Arnao I. Hepatoprotección antioxidante de la cáscara y semilla de Vitis vinifera L. (uva). An la Fac Med. 2013;69(4):250. Nisha D S, Sebastian B, Kalappurakkal R, Kirubakaran R. Efficacy of aloe vera and probiotic mouthwashes vs fluoride mouthwash on Streptococcus mutans in plaque around brackets of orthodontic patients: a randomized clinical trial. Angle Orthod. 2023;93(5):538–44 Eguizábal M, Nakata HM, Sanmarquina O. Actividad antibacteriana in vitro del extracto etanólico de propóleo peruano sobre Streptococcus mutans y Lactobacillus casei Artículo originAl. Med. 2007;10(2):18–20. 17. Hwang H-R, Lee E-S, Kang S-M, Chung K-H, Kim B-I. Effect of antimicrobial photodynamic therapy with Chlorella and Curcuma extract on Streptococcus mutans biofilms. Photodiagnosis Photodyn Ther. 2021;35:102411. Bazán Aliaga RB, Reátegui Arévalo Ó, Solórzano Espinoza LV, Castro Arredondo JA, Miranda García VE, Martínez Cadillo EE, et al. Actividad inhibitoria del extracto etanólico del Cyperus Rotundus procedente de la región de Cajamarca (provincia de Contumazá) en una cepa estandarizada de Streptococcus mutans (ATCC®25175). Rev Científica Odontológica. 2022;10(1):e093. Somanah J, Bourdon E, Bahorun T, Aruoma OI. The inhibitory effect of a fermented papaya preparation on growth, hydrophobicity, and acid production of Streptococcus mutans , Streptococcus mitis , and Lactobacillus acidophilus : its implications in oral health improvement of diabetics . Food Sci Nutr. 2013;1(6):416–21. Jeong J, Kim S, Huh C. Separation and Identification of an Antimicrobial Substance from Schisandra chinensis Extract against Streptococcus mutans. Molecules. 2023;28(8):3417. Caicedo Salazar J. Efecto Antimicrobiano De Extracto De Semilla De Uva (Vitis Vinifera) Sobre Cepas De Streptococcus Mutans: Estudio in Vitro. Universidad Central Del Ecuador; 2017. Thimothe J, Bonsi I, Padilla-Zakour O, Koo H. Chemical Characterization of Red Wine Grape (Vitis vinifera and Vitis Interspecific Hybrids) and Pomace Phenolic Extracts and Their Biological Activity against Streptococcus mutans. J Agric Food Chem. 2007;55:10200–7. Caicedo Salazar J, Mayorga Solórzano F, Montaño Tatés V, Salazar Garcés M, Del Carmen Armas A. Efecto antimicrobiano de extractos acuosos de la cáscara, pulpa y semilla de uva (vitis vinifera) sobre streptococcus mutans, estudio in vitro. Kiru. 2018;15(2):77–80. |
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Peláez Vargas, AlejandroMontoya Osorio, DanielaBarrera Pino, Ana MaríaCaballero Montoya, DanielaPeláez Vargas, AlejandroGIOM2024-06-12T18:22:17Z2028-06-112024-06-12T18:22:17Z2024-06-11Montoya Osorio, D.M., Barrera Pino, A.M., Caballero Montoya, D. y Peláez Vargas A. (2024). Evaluación del potencial antimicrobiano del extracto de cáscara, semilla y pulpa de la Vitis vinífera sobre el cultivo de S. mutans. [Tesis de pregrado, Universidad Cooperativa de Colombia]. Repositorio Institucional Universidad Cooperativa de Colombia.https://hdl.handle.net/20.500.12494/55914https://hdl.handle.net/20.500.12494/55914ABSTRACT Introduction: In the ongoing search for novel natural agents with potential health benefits, phenolic compounds have garnered significant attention due to their demonstrated antimicrobial and anti-inflammatory properties. Among these compounds, resveratrol and flavonoids, which are abundant in various plant sources, have shown promise in combating microbial infections and reducing inflammation. Materials and Methods: Three types of extracts were evaluated: One commercial and two experimental (aqueous and hydroalcoholic), prepared from the peel, seed, and pulp of Vitis vinifera. Two different dehydration temperatures were used. The S. mutans strain was selected and cultured on BHI agar plates, which were then treated with the extracts. Inhibition zones were measured at various times, with 0.2% chlorhexidine used as a positive control and sterile saline solution as a negative control. Results. No inhibition zones were found for any of the commercial extracts or experimental. Only inhibition zones were observed in the positive control of Chlorhexidine. Conclusions. The commercial extract and the hydroalcoholic and aqueous experimental extracts prepared from Vitis vinifera grape did not exhibit inhibition against S. mutans.Introducción: En la búsqueda continua de nuevos agentes naturales con posibles beneficios para la salud, los compuestos fenólicos han recibido atención significativa debido a sus demostradas propiedades antimicrobianas y antiinflamatorias. Entre estos compuestos, el resveratrol y los flavonoides, que son abundantes en varias fuentes vegetales, han mostrado promesa en la lucha contra las infecciones microbianas y la reducción de la inflamación. Materiales y Métodos: Se evaluaron tres tipos de extractos: uno comercial y dos experimentales (acuoso e hidroalcohólico), preparados a partir de la cáscara, semilla y pulpa de Vitis vinifera. Se utilizaron dos temperaturas de deshidratación diferentes. Se seleccionó la cepa de S. mutans y se cultivó en placas de agar BHI, que luego fueron tratadas con los extractos. Las zonas de inhibición se midieron en varios momentos, utilizando clorhexidina al 0,2% como control positivo y solución salina estéril como control negativo. Resultados: No se encontraron zonas de inhibición para ninguno de los extractos comerciales o experimentales. Solo se observaron zonas de inhibición en el control positivo de clorhexidina. Conclusiones: El extracto comercial y los extractos experimentales acuosos e hidroalcohólicos preparados a partir de uva Vitis vinifera no mostraron inhibición contra S. mutans.PregradoOdontólogoBiomaterials11 p.application/pdfspaUniversidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Odontología, Medellín y EnvigadoOdontologíaCiencias de la SaludMedellínMedellínhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://purl.org/coar/access_right/c_abf2Evaluación del potencial antimicrobiano del extracto de cáscara, semilla y pulpa de la Vitis vinífera sobre el cultivo de S. mutansTrabajo de grado - Pregradohttp://purl.org/coar/resource_type/c_7a1finfo:eu-repo/semantics/bachelorThesisinfo:eu-repo/semantics/acceptedVersionMolina-Quijada DMA, Medina-Juárez LA, González-Aguilar GA, Robles-Sánchez RM, Gámez-Meza N. Compuestos fenólicos y actividad antioxidante de cáscara de uva (Vitis vinifera L.) de mesa cultivada en el noroeste de México Phenolic compounds and antioxidant activity of table grape (Vitis vinifera L.) skin from northwest Mexico. CYTA - J Food. 2010;8(1):57–63.Nardini M. Compounds in Food: Characterization and Health Benefits. Molecules. 2022;25(27(3)):783.Wu CD. Grape products and oral health. J Nutr. 2009;139(9):3–8.Surco-laos F, Paucar HA, Gamboa WQ. Determinación De Polifenoles Totales Y Actividad Phenolic Compounds and Antioxidant Activity of Grape Seeds Residues of Pisco Production. Rev Soc Quim Perú. 2020;86(2):123–31.Clarke TK. Streptococcus mutans and dental caries. Br Med J. 1975;4(5997):647–8.Schindelin J, Arganda-Carrera I, Frise E, Verena K, Mark L, Tobias P, et al. Fiji - an Open platform for biological image analysis. Nat Methods. 2009;9(7).Aleixandre-Tudo JL, Buica A, Nieuwoudt H, Aleixandre JL, Du Toit W. Spectrophotometric Analysis of Phenolic Compounds in Grapes and Wines. J Agric Food Chem. 2017;65(20):4009–26.Reyes D, Ortega D, Quintero J, Piquer S, Alarcón M, Silva RF Da. Efecto antimicrobiano del extracto foliar de mango (Mangifera indica L. cv. Bocado) en microorganismos de interés clínico. Rev Salus [Internet]. 2017;21(2):7–13.Navarrete Barragán NA, Pita-Ospina EF, Sánchez Mora. RM, Giraldo Quintero SE, Bernal Lizarazú MC. Actividad in vitro de los extractos etanólicos de Lantana camara L., Petiveria alliacea L. y Lippia dulcis T. frente a bacterias patógenas. Nova. 2020;18(33):53–71.Castellanos JS, Betancourt DE, Báez DD, Baldión PA. Effect of flavonoids from grape seed and cranberry extracts on the microbiological activity of Streptococcus mutans : a systematic review of in vitro studies. BMC Oral Health. 2024;1–21.Estrada-Reyes R, Araujo-Escalona AG. Los flavonoides y el Sistema Nervioso Central Rosa. Salud Ment [Internet]. 2012;35(5):375–84.Sarduy-Bermúdez L, González-Díaz ME. La biopelícula: una nueva concepción de la placa dentobacteriana. Medicent Electrón. 2016;20(3):167–75.Cruz Quintana SM, Díaz Sjostrom P, Arias Socarrás D, Mazón Baldeón GM. Microbiota of oral cavity ecosystems. Rev Cubana Estomatol. 2017;54(1):84–99.Sandoval M, Lazarte K, Arnao I. Hepatoprotección antioxidante de la cáscara y semilla de Vitis vinifera L. (uva). An la Fac Med. 2013;69(4):250.Nisha D S, Sebastian B, Kalappurakkal R, Kirubakaran R. Efficacy of aloe vera and probiotic mouthwashes vs fluoride mouthwash on Streptococcus mutans in plaque around brackets of orthodontic patients: a randomized clinical trial. Angle Orthod. 2023;93(5):538–44Eguizábal M, Nakata HM, Sanmarquina O. Actividad antibacteriana in vitro del extracto etanólico de propóleo peruano sobre Streptococcus mutans y Lactobacillus casei Artículo originAl. Med. 2007;10(2):18–20. 17. Hwang H-R, Lee E-S, Kang S-M, Chung K-H, Kim B-I. Effect of antimicrobial photodynamic therapy with Chlorella and Curcuma extract on Streptococcus mutans biofilms. Photodiagnosis Photodyn Ther. 2021;35:102411.Bazán Aliaga RB, Reátegui Arévalo Ó, Solórzano Espinoza LV, Castro Arredondo JA, Miranda García VE, Martínez Cadillo EE, et al. Actividad inhibitoria del extracto etanólico del Cyperus Rotundus procedente de la región de Cajamarca (provincia de Contumazá) en una cepa estandarizada de Streptococcus mutans (ATCC®25175). Rev Científica Odontológica. 2022;10(1):e093.Somanah J, Bourdon E, Bahorun T, Aruoma OI. The inhibitory effect of a fermented papaya preparation on growth, hydrophobicity, and acid production of Streptococcus mutans , Streptococcus mitis , and Lactobacillus acidophilus : its implications in oral health improvement of diabetics . Food Sci Nutr. 2013;1(6):416–21.Jeong J, Kim S, Huh C. Separation and Identification of an Antimicrobial Substance from Schisandra chinensis Extract against Streptococcus mutans. Molecules. 2023;28(8):3417.Caicedo Salazar J. Efecto Antimicrobiano De Extracto De Semilla De Uva (Vitis Vinifera) Sobre Cepas De Streptococcus Mutans: Estudio in Vitro. Universidad Central Del Ecuador; 2017.Thimothe J, Bonsi I, Padilla-Zakour O, Koo H. Chemical Characterization of Red Wine Grape (Vitis vinifera and Vitis Interspecific Hybrids) and Pomace Phenolic Extracts and Their Biological Activity against Streptococcus mutans. J Agric Food Chem. 2007;55:10200–7.Caicedo Salazar J, Mayorga Solórzano F, Montaño Tatés V, Salazar Garcés M, Del Carmen Armas A. Efecto antimicrobiano de extractos acuosos de la cáscara, pulpa y semilla de uva (vitis vinifera) sobre streptococcus mutans, estudio in vitro. 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