Partial characterization of venom from the Colombian spider Phoneutria boliviensis (Aranae:Ctenidae)

We report on the first studies on the characterization of venom from Phoneutria boliviensis (Aranae:Ctenidae) (F. O. Pickard-Cambridge, 1897), done with Colombian species. After the electrostimulation extraction process, the venom showed physicochemical properties corresponding to a colorless and wa...

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Autores:
Estrada Gomez, S.
Vargas Muñoz, Leidy Johana
Lanchero, P.
Latorre, C. S.
Tipo de recurso:
Article of journal
Fecha de publicación:
2023
Institución:
Universidad Cooperativa de Colombia
Repositorio:
Repositorio UCC
Idioma:
OAI Identifier:
oai:repository.ucc.edu.co:20.500.12494/49648
Acceso en línea:
https://doi.org/10.3390/toxins7082872
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84940857999&doi=10.3390%2ftoxins7082872&partnerID=40&md5=ed1ae486f3e0aef1480fc6f4ffeda62d
https://hdl.handle.net/20.500.12494/49648
Palabra clave:
AEDES
ANIMAL
ANIMALS
ARTICLE
BENZOYLARGININE 4 NITROANILIDE
BENZOYLARGININE NITROANILIDE
CHEMISTRY
CHROMATOGRAPHY, HIGH PRESSURE LIQUID
DRUG EFFECTS
ELECTROSPRAY MASS SPECTROMETRY
ENZYME ACTIVITY
FEMALE
FREEZE DRYING
HEMOLYSIS
HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
INSECTICIDAL ACTIVITY
LARVA
MALE
MASS SPECTROMETRY
METABOLISM
NONHUMAN
PEPTIDE
PEPTIDES
PHONEUTRIA
PHOSPHOLIPASE A2
PHOSPHOLIPASES A2
PHYSICAL CHEMISTRY
POLYACRYLAMIDE GEL ELECTROPHORESIS
PROTEIN ANALYSIS
PROTEIN CONTENT
PROTEIN DEGRADATION
PROTEOLYSIS
PROTEOMICS
REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION
SPIDER
SPIDER VENOM
SPIDER VENOMS
SPIDERS
TANDEM MASS SPECTROMETRY
Rights
openAccess
License
http://purl.org/coar/access_right/c_abf2
id COOPER2_fd15b553ca9a5afc2fe4e678f66f1c9f
oai_identifier_str oai:repository.ucc.edu.co:20.500.12494/49648
network_acronym_str COOPER2
network_name_str Repositorio UCC
repository_id_str
spelling Estrada Gomez, S.Vargas Muñoz, Leidy JohanaLanchero, P.Latorre, C. S.2023-05-24T16:21:36Z2023-05-24T16:21:36Z31/07/2015https://doi.org/10.3390/toxins7082872https://www.scopus.com/inward/record.uri?eid=2-s2.0-84940857999&doi=10.3390%2ftoxins7082872&partnerID=40&md5=ed1ae486f3e0aef1480fc6f4ffeda62d20726651https://hdl.handle.net/20.500.12494/49648Estrada-Gomez S.,Vargas Muñoz Leidy johana,Lanchero P.,Latorre C.S..Partial characterization of venom from the Colombian spider Phoneutria boliviensis (Aranae:Ctenidae).Toxins. 2015. 7. (8):p. 2872-2887We report on the first studies on the characterization of venom from Phoneutria boliviensis (Aranae:Ctenidae) (F. O. Pickard-Cambridge, 1897), done with Colombian species. After the electrostimulation extraction process, the venom showed physicochemical properties corresponding to a colorless and water-soluble liquid with a density of 0.86 mg/mL and 87% aqueous content. P. boliviensis venom and RP-HPLC fractions showed hemolytic activity and hydrolyzed the synthetic substrate 4-nitro-3-octanoyloxy-benzoic acid, indicating the presence of phospholipases A2 enzymes. The electrophoretic profile showed an important protein content with molecular masses below 14 kDa, and differences between male and female protein content were also revealed. The RP-HPLC venom profile exposes differences between males and female content consistent with the electrophoretic profile. Five fractions collected from the RP-HPLC displayed significant larvicidal activity. Mass analysis indicates the presence of peptides ranging from 1047.71 to 3278.07 Da. Two peptides, Ctenitoxin-Pb48 and Ctenitoxin-Pb53, were partially identified using HPLC-nESI-MS/MS, which showed a high homology with other Ctenitoxins (family Tx3) from Phoneutria nigriventer, Phoneutria keyserlingi and Phoneutria reidyi affecting voltage-gated calcium receptors (Cav 1, 2.1, 2.2 and 2.3) and NMDA-glutamate receptors. © 2015 by the authors; licensee MDPI, Basel, Switzerland.leidy.vargasmu@campusucc.edu.co2872-2887MDPI AGAEDESANIMALANIMALSARTICLEBENZOYLARGININE 4 NITROANILIDEBENZOYLARGININE NITROANILIDECHEMISTRYCHROMATOGRAPHY, HIGH PRESSURE LIQUIDDRUG EFFECTSELECTROSPRAY MASS SPECTROMETRYENZYME ACTIVITYFEMALEFREEZE DRYINGHEMOLYSISHIGH PERFORMANCE LIQUID CHROMATOGRAPHYINSECTICIDAL ACTIVITYLARVAMALEMASS SPECTROMETRYMETABOLISMNONHUMANPEPTIDEPEPTIDESPHONEUTRIAPHOSPHOLIPASE A2PHOSPHOLIPASES A2PHYSICAL CHEMISTRYPOLYACRYLAMIDE GEL ELECTROPHORESISPROTEIN ANALYSISPROTEIN CONTENTPROTEIN DEGRADATIONPROTEOLYSISPROTEOMICSREVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHYSPECTROMETRY, MASS, ELECTROSPRAY IONIZATIONSPIDERSPIDER VENOMSPIDER VENOMSSPIDERSTANDEM MASS SPECTROMETRYPartial characterization of venom from the Colombian spider Phoneutria boliviensis (Aranae:Ctenidae)Artículohttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionToxinsinfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Publication20.500.12494/49648oai:repository.ucc.edu.co:20.500.12494/496482024-08-20 16:17:34.295metadata.onlyhttps://repository.ucc.edu.coRepositorio Institucional Universidad Cooperativa de Colombiabdigital@metabiblioteca.com
dc.title.spa.fl_str_mv Partial characterization of venom from the Colombian spider Phoneutria boliviensis (Aranae:Ctenidae)
title Partial characterization of venom from the Colombian spider Phoneutria boliviensis (Aranae:Ctenidae)
spellingShingle Partial characterization of venom from the Colombian spider Phoneutria boliviensis (Aranae:Ctenidae)
AEDES
ANIMAL
ANIMALS
ARTICLE
BENZOYLARGININE 4 NITROANILIDE
BENZOYLARGININE NITROANILIDE
CHEMISTRY
CHROMATOGRAPHY, HIGH PRESSURE LIQUID
DRUG EFFECTS
ELECTROSPRAY MASS SPECTROMETRY
ENZYME ACTIVITY
FEMALE
FREEZE DRYING
HEMOLYSIS
HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
INSECTICIDAL ACTIVITY
LARVA
MALE
MASS SPECTROMETRY
METABOLISM
NONHUMAN
PEPTIDE
PEPTIDES
PHONEUTRIA
PHOSPHOLIPASE A2
PHOSPHOLIPASES A2
PHYSICAL CHEMISTRY
POLYACRYLAMIDE GEL ELECTROPHORESIS
PROTEIN ANALYSIS
PROTEIN CONTENT
PROTEIN DEGRADATION
PROTEOLYSIS
PROTEOMICS
REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION
SPIDER
SPIDER VENOM
SPIDER VENOMS
SPIDERS
TANDEM MASS SPECTROMETRY
title_short Partial characterization of venom from the Colombian spider Phoneutria boliviensis (Aranae:Ctenidae)
title_full Partial characterization of venom from the Colombian spider Phoneutria boliviensis (Aranae:Ctenidae)
title_fullStr Partial characterization of venom from the Colombian spider Phoneutria boliviensis (Aranae:Ctenidae)
title_full_unstemmed Partial characterization of venom from the Colombian spider Phoneutria boliviensis (Aranae:Ctenidae)
title_sort Partial characterization of venom from the Colombian spider Phoneutria boliviensis (Aranae:Ctenidae)
dc.creator.fl_str_mv Estrada Gomez, S.
Vargas Muñoz, Leidy Johana
Lanchero, P.
Latorre, C. S.
dc.contributor.author.none.fl_str_mv Estrada Gomez, S.
Vargas Muñoz, Leidy Johana
Lanchero, P.
Latorre, C. S.
dc.subject.spa.fl_str_mv AEDES
ANIMAL
ANIMALS
ARTICLE
BENZOYLARGININE 4 NITROANILIDE
BENZOYLARGININE NITROANILIDE
CHEMISTRY
CHROMATOGRAPHY, HIGH PRESSURE LIQUID
DRUG EFFECTS
ELECTROSPRAY MASS SPECTROMETRY
ENZYME ACTIVITY
FEMALE
FREEZE DRYING
HEMOLYSIS
HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
INSECTICIDAL ACTIVITY
LARVA
MALE
MASS SPECTROMETRY
METABOLISM
NONHUMAN
PEPTIDE
PEPTIDES
PHONEUTRIA
PHOSPHOLIPASE A2
PHOSPHOLIPASES A2
PHYSICAL CHEMISTRY
POLYACRYLAMIDE GEL ELECTROPHORESIS
PROTEIN ANALYSIS
PROTEIN CONTENT
PROTEIN DEGRADATION
PROTEOLYSIS
PROTEOMICS
REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION
SPIDER
SPIDER VENOM
SPIDER VENOMS
SPIDERS
TANDEM MASS SPECTROMETRY
topic AEDES
ANIMAL
ANIMALS
ARTICLE
BENZOYLARGININE 4 NITROANILIDE
BENZOYLARGININE NITROANILIDE
CHEMISTRY
CHROMATOGRAPHY, HIGH PRESSURE LIQUID
DRUG EFFECTS
ELECTROSPRAY MASS SPECTROMETRY
ENZYME ACTIVITY
FEMALE
FREEZE DRYING
HEMOLYSIS
HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
INSECTICIDAL ACTIVITY
LARVA
MALE
MASS SPECTROMETRY
METABOLISM
NONHUMAN
PEPTIDE
PEPTIDES
PHONEUTRIA
PHOSPHOLIPASE A2
PHOSPHOLIPASES A2
PHYSICAL CHEMISTRY
POLYACRYLAMIDE GEL ELECTROPHORESIS
PROTEIN ANALYSIS
PROTEIN CONTENT
PROTEIN DEGRADATION
PROTEOLYSIS
PROTEOMICS
REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION
SPIDER
SPIDER VENOM
SPIDER VENOMS
SPIDERS
TANDEM MASS SPECTROMETRY
description We report on the first studies on the characterization of venom from Phoneutria boliviensis (Aranae:Ctenidae) (F. O. Pickard-Cambridge, 1897), done with Colombian species. After the electrostimulation extraction process, the venom showed physicochemical properties corresponding to a colorless and water-soluble liquid with a density of 0.86 mg/mL and 87% aqueous content. P. boliviensis venom and RP-HPLC fractions showed hemolytic activity and hydrolyzed the synthetic substrate 4-nitro-3-octanoyloxy-benzoic acid, indicating the presence of phospholipases A2 enzymes. The electrophoretic profile showed an important protein content with molecular masses below 14 kDa, and differences between male and female protein content were also revealed. The RP-HPLC venom profile exposes differences between males and female content consistent with the electrophoretic profile. Five fractions collected from the RP-HPLC displayed significant larvicidal activity. Mass analysis indicates the presence of peptides ranging from 1047.71 to 3278.07 Da. Two peptides, Ctenitoxin-Pb48 and Ctenitoxin-Pb53, were partially identified using HPLC-nESI-MS/MS, which showed a high homology with other Ctenitoxins (family Tx3) from Phoneutria nigriventer, Phoneutria keyserlingi and Phoneutria reidyi affecting voltage-gated calcium receptors (Cav 1, 2.1, 2.2 and 2.3) and NMDA-glutamate receptors. © 2015 by the authors; licensee MDPI, Basel, Switzerland.
publishDate 2023
dc.date.accessioned.none.fl_str_mv 2023-05-24T16:21:36Z
dc.date.available.none.fl_str_mv 2023-05-24T16:21:36Z
dc.date.issued.none.fl_str_mv 31/07/2015
dc.type.none.fl_str_mv Artículo
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.coar.none.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.coarversion.none.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.driver.none.fl_str_mv info:eu-repo/semantics/article
dc.type.redcol.none.fl_str_mv http://purl.org/redcol/resource_type/ART
dc.type.version.none.fl_str_mv info:eu-repo/semantics/publishedVersion
format http://purl.org/coar/resource_type/c_6501
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.3390/toxins7082872
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84940857999&doi=10.3390%2ftoxins7082872&partnerID=40&md5=ed1ae486f3e0aef1480fc6f4ffeda62d
dc.identifier.issn.spa.fl_str_mv 20726651
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/20.500.12494/49648
dc.identifier.bibliographicCitation.spa.fl_str_mv Estrada-Gomez S.,Vargas Muñoz Leidy johana,Lanchero P.,Latorre C.S..Partial characterization of venom from the Colombian spider Phoneutria boliviensis (Aranae:Ctenidae).Toxins. 2015. 7. (8):p. 2872-2887
url https://doi.org/10.3390/toxins7082872
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84940857999&doi=10.3390%2ftoxins7082872&partnerID=40&md5=ed1ae486f3e0aef1480fc6f4ffeda62d
https://hdl.handle.net/20.500.12494/49648
identifier_str_mv 20726651
Estrada-Gomez S.,Vargas Muñoz Leidy johana,Lanchero P.,Latorre C.S..Partial characterization of venom from the Colombian spider Phoneutria boliviensis (Aranae:Ctenidae).Toxins. 2015. 7. (8):p. 2872-2887
dc.relation.ispartofjournal.spa.fl_str_mv Toxins
dc.rights.accessrights.none.fl_str_mv info:eu-repo/semantics/openAccess
dc.rights.coar.none.fl_str_mv http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
rights_invalid_str_mv http://purl.org/coar/access_right/c_abf2
dc.format.extent.spa.fl_str_mv 2872-2887
dc.publisher.spa.fl_str_mv MDPI AG
institution Universidad Cooperativa de Colombia
repository.name.fl_str_mv Repositorio Institucional Universidad Cooperativa de Colombia
repository.mail.fl_str_mv bdigital@metabiblioteca.com
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