Partial characterization of venom from the Colombian spider Phoneutria boliviensis (Aranae:Ctenidae)

We report on the first studies on the characterization of venom from Phoneutria boliviensis (Aranae:Ctenidae) (F. O. Pickard-Cambridge, 1897), done with Colombian species. After the electrostimulation extraction process, the venom showed physicochemical properties corresponding to a colorless and wa...

Full description

Autores:
Estrada Gomez, S.
Vargas Muñoz, Leidy Johana
Lanchero, P.
Latorre, C. S.
Tipo de recurso:
Article of journal
Fecha de publicación:
2023
Institución:
Universidad Cooperativa de Colombia
Repositorio:
Repositorio UCC
Idioma:
OAI Identifier:
oai:repository.ucc.edu.co:20.500.12494/49648
Acceso en línea:
https://doi.org/10.3390/toxins7082872
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84940857999&doi=10.3390%2ftoxins7082872&partnerID=40&md5=ed1ae486f3e0aef1480fc6f4ffeda62d
https://hdl.handle.net/20.500.12494/49648
Palabra clave:
AEDES
ANIMAL
ANIMALS
ARTICLE
BENZOYLARGININE 4 NITROANILIDE
BENZOYLARGININE NITROANILIDE
CHEMISTRY
CHROMATOGRAPHY, HIGH PRESSURE LIQUID
DRUG EFFECTS
ELECTROSPRAY MASS SPECTROMETRY
ENZYME ACTIVITY
FEMALE
FREEZE DRYING
HEMOLYSIS
HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
INSECTICIDAL ACTIVITY
LARVA
MALE
MASS SPECTROMETRY
METABOLISM
NONHUMAN
PEPTIDE
PEPTIDES
PHONEUTRIA
PHOSPHOLIPASE A2
PHOSPHOLIPASES A2
PHYSICAL CHEMISTRY
POLYACRYLAMIDE GEL ELECTROPHORESIS
PROTEIN ANALYSIS
PROTEIN CONTENT
PROTEIN DEGRADATION
PROTEOLYSIS
PROTEOMICS
REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION
SPIDER
SPIDER VENOM
SPIDER VENOMS
SPIDERS
TANDEM MASS SPECTROMETRY
Rights
openAccess
License
http://purl.org/coar/access_right/c_abf2
Description
Summary:We report on the first studies on the characterization of venom from Phoneutria boliviensis (Aranae:Ctenidae) (F. O. Pickard-Cambridge, 1897), done with Colombian species. After the electrostimulation extraction process, the venom showed physicochemical properties corresponding to a colorless and water-soluble liquid with a density of 0.86 mg/mL and 87% aqueous content. P. boliviensis venom and RP-HPLC fractions showed hemolytic activity and hydrolyzed the synthetic substrate 4-nitro-3-octanoyloxy-benzoic acid, indicating the presence of phospholipases A2 enzymes. The electrophoretic profile showed an important protein content with molecular masses below 14 kDa, and differences between male and female protein content were also revealed. The RP-HPLC venom profile exposes differences between males and female content consistent with the electrophoretic profile. Five fractions collected from the RP-HPLC displayed significant larvicidal activity. Mass analysis indicates the presence of peptides ranging from 1047.71 to 3278.07 Da. Two peptides, Ctenitoxin-Pb48 and Ctenitoxin-Pb53, were partially identified using HPLC-nESI-MS/MS, which showed a high homology with other Ctenitoxins (family Tx3) from Phoneutria nigriventer, Phoneutria keyserlingi and Phoneutria reidyi affecting voltage-gated calcium receptors (Cav 1, 2.1, 2.2 and 2.3) and NMDA-glutamate receptors. © 2015 by the authors; licensee MDPI, Basel, Switzerland.