Facultative anaerobic bacteria on dentistry students’ gutta-percha points: The importance of disinfection

Background: During endodontic treatment in dentistry, if the gutta-percha points contain microorganisms that are resistant to the conditions in the root canal once it is sealed, they can lead to new infections. The purpose of this study was to determine the presence and quantification of facultative...

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Autores:: Universidad Cooperativa de Colombia
Angarita Díaz, María del Pilar
Rozo Ortiz, Diana Carolina
Forero Escobar, Diana
Arias Ubaque, Andrea Isabel
Imbachi Lizcano, Alvaro
Laidy Johanna, Sandoval Diaz

Tipo de recurso:: http://purl.org/coar/resource_type/c_f744

Fecha de publicación:: 2018

Institución:: Universidad Cooperativa de Colombia

Repositorio:: Repositorio UCC

Idioma:

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dc.title.spa.fl_str_mv	Facultative anaerobic bacteria on dentistry students’ gutta-percha points: The importance of disinfection
title	Facultative anaerobic bacteria on dentistry students’ gutta-percha points: The importance of disinfection
spellingShingle	Facultative anaerobic bacteria on dentistry students’ gutta-percha points: The importance of disinfection Gutta-percha obturación del conducto radicular contaminación bacteria TG 2018 ODO Gutta-percha root canal obturation contamination bacteria
title_short	Facultative anaerobic bacteria on dentistry students’ gutta-percha points: The importance of disinfection
title_full	Facultative anaerobic bacteria on dentistry students’ gutta-percha points: The importance of disinfection
title_fullStr	Facultative anaerobic bacteria on dentistry students’ gutta-percha points: The importance of disinfection
title_full_unstemmed	Facultative anaerobic bacteria on dentistry students’ gutta-percha points: The importance of disinfection
title_sort	Facultative anaerobic bacteria on dentistry students’ gutta-percha points: The importance of disinfection
dc.creator.fl_str_mv	Universidad Cooperativa de Colombia Angarita Díaz, María del Pilar Rozo Ortiz, Diana Carolina Forero Escobar, Diana Arias Ubaque, Andrea Isabel Imbachi Lizcano, Alvaro Laidy Johanna, Sandoval Diaz
dc.contributor.advisor.none.fl_str_mv	Amirov, Chingiz M.
dc.contributor.author.none.fl_str_mv	Universidad Cooperativa de Colombia Angarita Díaz, María del Pilar Rozo Ortiz, Diana Carolina Forero Escobar, Diana Arias Ubaque, Andrea Isabel Imbachi Lizcano, Alvaro Laidy Johanna, Sandoval Diaz
dc.subject.spa.fl_str_mv	Gutta-percha obturación del conducto radicular contaminación bacteria
topic	Gutta-percha obturación del conducto radicular contaminación bacteria TG 2018 ODO Gutta-percha root canal obturation contamination bacteria
dc.subject.classification.spa.fl_str_mv	TG 2018 ODO
dc.subject.other.spa.fl_str_mv	Gutta-percha root canal obturation contamination bacteria
description	Background: During endodontic treatment in dentistry, if the gutta-percha points contain microorganisms that are resistant to the conditions in the root canal once it is sealed, they can lead to new infections. The purpose of this study was to determine the presence and quantification of facultative anaerobic bacteria in students’ guttapercha points. Methods: A representative sample of dentistry students’ gutta-percha points were collected, together with information on their characteristics. The points were placed in saline solution for inoculation in blood agar followed by anaerobic incubation for five days. Bacteria presence and type were determined, quantified, and identified. Following this, the X2 test was used to verify whether there were any significant differences in the contamination found in the points between the characteristics studied. Results: The results of the microbiological analysis revealed that 32.1% of the points appeared to be contaminated by facultative anaerobic bacteria. The most common types of microorganisms were gram-positive bacilli, followed by gram-positive coccus. Staphylococcus epidermidis was among the microorganisms identified. No relationship was observed between the presence of cone contamination and the characteristics, but a statistically significant difference was detected within the group defined by package opening date. Significant differences were also found in terms of the presence of spore-forming bacilli within the group defined by package opening and expiry date. Conclusions: The presence of facultative anaerobic bacteria of clinical interest in the gutta-percha points used by dentistry students was identified and quantified. Among the bacteria identified, some are of clinical importance, such as Staphylococcus epidermidis and Streptococcus mitis. The establishment of disinfection protocols for such materials is recommended.
publishDate	2018
dc.date.issued.none.fl_str_mv	2018-12-01
dc.date.accessioned.none.fl_str_mv	2020-01-15T16:17:01Z
dc.date.available.none.fl_str_mv	2020-01-15T16:17:01Z
dc.type.none.fl_str_mv	Acta de memorias
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dc.identifier.issn.spa.fl_str_mv	11835702
dc.identifier.uri.spa.fl_str_mv	N/A
dc.identifier.uri.none.fl_str_mv	https://hdl.handle.net/20.500.12494/15924
dc.identifier.bibliographicCitation.spa.fl_str_mv	Angarita Díaz M. P., Rozo D. C., Forero D., Arias Ubaque, A.I., Imbachi, A. y Sandoval, L. J. (2018). Facultative anaerobic bacteria on dentistry students’ gutta-percha points: The importance of disinfection. Canadian Journal of Infection Control. 33(4):223-226. Recuperado de:
identifier_str_mv	11835702 N/A Angarita Díaz M. P., Rozo D. C., Forero D., Arias Ubaque, A.I., Imbachi, A. y Sandoval, L. J. (2018). Facultative anaerobic bacteria on dentistry students’ gutta-percha points: The importance of disinfection. Canadian Journal of Infection Control. 33(4):223-226. Recuperado de:
url	https://hdl.handle.net/20.500.12494/15924
dc.relation.isversionof.spa.fl_str_mv	https://ipac-canada.org/photos/custom/CJIC/CJIC_Winter_2018_Angarita.pdf
dc.relation.ispartofjournal.spa.fl_str_mv	Canadian Journal of Infection Control
dc.relation.references.spa.fl_str_mv	Abbott, P. V., & Salgado, J. C. (2009). Strategies for the endodontic management of concurrent endodontic and periodontal diseases. Australian Dental Journal, 54(Suppl 1), S70-S85. Farber, P. A., & Seltzer, S. (1988). Endodontic microbiology. I. Etiology. Journal of Endodontics, 14(7), 363-371 Baumgartner, J. C., Bakland, L. K., & Sugita, E. I. (2002). Microbiology of endodontics and asepsis in endodontic practice. In J. I. Ingle, & C. Baumgartner (Eds.), Ingle’s endodontics (63-88). London, UK: BC Decker. Leggett, H. C., Cornwallis, C. K., & West, S. A. (2012). Mechanisms of pathogenesis, infective dose and virulence in human parasites. PLoS Pathogens, 8(2), 10-12. Shailaja, S., & Suresh, B. S. (2014). Endodontic microflora – A review. Journal of Oral Health and Community Dentistry, 8(3), 160-165. Siqueira, Jr., J. F. (2001). Aetiology of root canal treatment failure: Why well-treated teeth can bail. International Endodontic Journal, 34(1), 1-10. Anderson, A. C., Hellwig, E., Vespermann, R., Wittmer, A., Schmid, M., Karygianni, L., & Al-Ahmad, A. (2012). Comprehensive analysis of secondary dental root canal infections: A combination of culture and culture-independent approaches reveals new insights. PLoS One, 7(11), e49576. Pandey, V., Choudhary, I., Kumar, V., Tripathi, P., Misra, A., & Bagde, H. (2016). Assessment of correlation between clinical parameters and pulp canal pathogens in endodontic pathologies: A microbiological study. Journal of Contemporary Dental Practice, 17(8), 654-658. Hentges, D. J. (1996). Anaerobes: General characteristics. In S. Baron (Ed.), Medical microbiology (4th ed.). Galveston, TX: University of Texas Medical Branch. Retrieved from https://www.ncbi.nlm.nih.gov/books/ NBK7638/ Pang, N. S., Jung, I. Y., Bae, K. S., Baek, S. H., Lee, W. C., & Kum, K. Y. (2007). Effects of short-term chemical disinfection of gutta-percha cones: Identification of affected microbes and alterations in surface texture and physical properties. Journal of Endodontics, 33(5), 594-598. Nabeshima, C. K., de Lima Machado, M. E., Borges Britto, M. L., & Pallotta, R. C. (2011). Effectiveness of different chemical agents for disinfection of gutta-percha cones. Australian Endodontic Journal, 37(3), 118-121. Gomes, B. P., Vianna, M. E., Matsumoto, C. U., Rossi, V. de P., Zaia, A. A., Ferraz, C. C., & Souza, F. J. (2005). Disinfection of gutta-percha cones with chlorhexidine and sodium hypochlorite. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics, 100(4), 512-517. Kayaoglu, G., Gürel, M., Omürlü, H., Bek, Z. G., & Sadik, B. (2009). Examination of gutta-percha cones for microbial contamination during chemical use. Journal of Applied Oral Science, 17(3), 244-247. Shweta, S. K. P., & Prakash, S. K. (2013). Dental abscess: A microbiological review. Dental Research Journal, 10(5), 585-591. Otto, M. (2009). Staphylococcus epidermidis – the “accidental” pathogen. Nature Reviews Microbiology, 7(8), 555-567. Blum, R. A., & Rodvold, K. A. (1987). Recognition and importance of Staphylococcus epidermidis infections. Clinical Pharmacy, 6(6), 464-475. Rôças, I. N., & Siqueira, Jr., J. F. (2012). Characterization of microbiota of root canal-treated teeth with posttreatment disease. Journal of Clinical Microbiology, 50(5), 1721-1724. O’Gara, J. P., & Humphreys, H. (2001). Staphylococcus epidermidis biofilms: Importance and implications. Journal of Medical Microbiology, 50(7), 582-587. Otto, M. (2014). Staphylococcus epidermidis pathogenesis. Methods in Molecular Biology, 1106, 17-31. Mitchell, J. (2011). Streptococcus mitis: Walking the line between commensalism and pathogenesis. Molecular Oral Microbiology, 26(2), 89-98. Siqueira, Jr., J. F., & Rôças, I. N. (2006). Catonella morbi and Granulicatella adiacens: New species in endodontic infections. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics, 102(2), 259-264. Shailaja, T. S., Sathiavathy, K. A., & Unni, G. (2013). Infective endocarditis caused by Granulicatella adiacens. Indian Heart Journal, 65(4), 447-449. Cargill, J. S., Scott, K. S., Gascoyne-Binzi, D., & Sandoe, J. A. T. (2012). Granulicatella infection: Diagnosis and management. Journal of Medical Microbiology, 61(6), 755-761. Turnbull, P. C. B. (1996). Bacillus. In S. Baron (Ed.), Medical microbiology (4th ed.). Galveston, TX: University of Texas Medical Branch. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/21413260 Pan, J., Zhao, J., & Jiang, N. (2014). Oral cavity infection: An adverse effect after the treatment of oral cancer in aged individuals. Journal of Applied Oral Science, 22(4), 261-267.
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dc.coverage.temporal.spa.fl_str_mv	Volumen 33 Número 4
dc.publisher.spa.fl_str_mv	Amirov Chingiz M. Universidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Programa de Odontología, Villavicencio, Colombia, 00000
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spelling	Amirov, Chingiz M.Universidad Cooperativa de ColombiaAngarita Díaz, María del PilarRozo Ortiz, Diana CarolinaForero Escobar, DianaArias Ubaque, Andrea IsabelImbachi Lizcano, AlvaroLaidy Johanna, Sandoval DiazVolumen 33 Número 42020-01-15T16:17:01Z2020-01-15T16:17:01Z2018-12-0111835702N/Ahttps://hdl.handle.net/20.500.12494/15924Angarita Díaz M. P., Rozo D. C., Forero D., Arias Ubaque, A.I., Imbachi, A. y Sandoval, L. J. (2018). Facultative anaerobic bacteria on dentistry students’ gutta-percha points: The importance of disinfection. Canadian Journal of Infection Control. 33(4):223-226. Recuperado de:Background: During endodontic treatment in dentistry, if the gutta-percha points contain microorganisms that are resistant to the conditions in the root canal once it is sealed, they can lead to new infections. The purpose of this study was to determine the presence and quantification of facultative anaerobic bacteria in students’ guttapercha points. Methods: A representative sample of dentistry students’ gutta-percha points were collected, together with information on their characteristics. The points were placed in saline solution for inoculation in blood agar followed by anaerobic incubation for five days. Bacteria presence and type were determined, quantified, and identified. Following this, the X2 test was used to verify whether there were any significant differences in the contamination found in the points between the characteristics studied. Results: The results of the microbiological analysis revealed that 32.1% of the points appeared to be contaminated by facultative anaerobic bacteria. The most common types of microorganisms were gram-positive bacilli, followed by gram-positive coccus. Staphylococcus epidermidis was among the microorganisms identified. No relationship was observed between the presence of cone contamination and the characteristics, but a statistically significant difference was detected within the group defined by package opening date. Significant differences were also found in terms of the presence of spore-forming bacilli within the group defined by package opening and expiry date. Conclusions: The presence of facultative anaerobic bacteria of clinical interest in the gutta-percha points used by dentistry students was identified and quantified. Among the bacteria identified, some are of clinical importance, such as Staphylococcus epidermidis and Streptococcus mitis. The establishment of disinfection protocols for such materials is recommended.http://scienti.colciencias.gov.co:8081/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0001561382https://orcid.org/0000-0002-5435-3456GIOMETmaria.angaritad@campusucc.edu.codiana.rozo@campusucc.edu.codiana.foreroe@ucc.edu.coandrea.ariasu@hotmail.comalimliz1025@hotmail.comlaidysandoval@outlook.eshttp://scienti.colciencias.gov.co:8085/gruplac/jsp/visualiza/visualizagr.jsp?nro=000000000094414Amirov Chingiz M.Universidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Programa de Odontología, Villavicencio, Colombia, 00000OdontologíaVillavicenciohttps://ipac-canada.org/photos/custom/CJIC/CJIC_Winter_2018_Angarita.pdfCanadian Journal of Infection ControlAbbott, P. V., & Salgado, J. C. (2009). Strategies for the endodontic management of concurrent endodontic and periodontal diseases. Australian Dental Journal, 54(Suppl 1), S70-S85.Farber, P. A., & Seltzer, S. (1988). Endodontic microbiology. I. Etiology. Journal of Endodontics, 14(7), 363-371Baumgartner, J. C., Bakland, L. K., & Sugita, E. I. (2002). Microbiology of endodontics and asepsis in endodontic practice. In J. I. Ingle, & C. Baumgartner (Eds.), Ingle’s endodontics (63-88). London, UK: BC Decker.Leggett, H. C., Cornwallis, C. K., & West, S. A. (2012). Mechanisms of pathogenesis, infective dose and virulence in human parasites. PLoS Pathogens, 8(2), 10-12.Shailaja, S., & Suresh, B. S. (2014). Endodontic microflora – A review. Journal of Oral Health and Community Dentistry, 8(3), 160-165.Siqueira, Jr., J. F. (2001). Aetiology of root canal treatment failure: Why well-treated teeth can bail. International Endodontic Journal, 34(1), 1-10.Anderson, A. C., Hellwig, E., Vespermann, R., Wittmer, A., Schmid, M., Karygianni, L., & Al-Ahmad, A. (2012). Comprehensive analysis of secondary dental root canal infections: A combination of culture and culture-independent approaches reveals new insights. PLoS One, 7(11), e49576.Pandey, V., Choudhary, I., Kumar, V., Tripathi, P., Misra, A., & Bagde, H. (2016). Assessment of correlation between clinical parameters and pulp canal pathogens in endodontic pathologies: A microbiological study. Journal of Contemporary Dental Practice, 17(8), 654-658.Hentges, D. J. (1996). Anaerobes: General characteristics. In S. Baron (Ed.), Medical microbiology (4th ed.). Galveston, TX: University of Texas Medical Branch. Retrieved from https://www.ncbi.nlm.nih.gov/books/ NBK7638/Pang, N. S., Jung, I. Y., Bae, K. S., Baek, S. H., Lee, W. C., & Kum, K. Y. (2007). Effects of short-term chemical disinfection of gutta-percha cones: Identification of affected microbes and alterations in surface texture and physical properties. Journal of Endodontics, 33(5), 594-598.Nabeshima, C. K., de Lima Machado, M. E., Borges Britto, M. L., & Pallotta, R. C. (2011). Effectiveness of different chemical agents for disinfection of gutta-percha cones. Australian Endodontic Journal, 37(3), 118-121.Gomes, B. P., Vianna, M. E., Matsumoto, C. U., Rossi, V. de P., Zaia, A. A., Ferraz, C. C., & Souza, F. J. (2005). Disinfection of gutta-percha cones with chlorhexidine and sodium hypochlorite. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics, 100(4), 512-517.Kayaoglu, G., Gürel, M., Omürlü, H., Bek, Z. G., & Sadik, B. (2009). Examination of gutta-percha cones for microbial contamination during chemical use. Journal of Applied Oral Science, 17(3), 244-247.Shweta, S. K. P., & Prakash, S. K. (2013). Dental abscess: A microbiological review. Dental Research Journal, 10(5), 585-591.Otto, M. (2009). Staphylococcus epidermidis – the “accidental” pathogen. Nature Reviews Microbiology, 7(8), 555-567.Blum, R. A., & Rodvold, K. A. (1987). Recognition and importance of Staphylococcus epidermidis infections. Clinical Pharmacy, 6(6), 464-475.Rôças, I. N., & Siqueira, Jr., J. F. (2012). Characterization of microbiota of root canal-treated teeth with posttreatment disease. Journal of Clinical Microbiology, 50(5), 1721-1724.O’Gara, J. P., & Humphreys, H. (2001). Staphylococcus epidermidis biofilms: Importance and implications. Journal of Medical Microbiology, 50(7), 582-587.Otto, M. (2014). Staphylococcus epidermidis pathogenesis. Methods in Molecular Biology, 1106, 17-31.Mitchell, J. (2011). Streptococcus mitis: Walking the line between commensalism and pathogenesis. Molecular Oral Microbiology, 26(2), 89-98.Siqueira, Jr., J. F., & Rôças, I. N. (2006). Catonella morbi and Granulicatella adiacens: New species in endodontic infections. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics, 102(2), 259-264.Shailaja, T. S., Sathiavathy, K. A., & Unni, G. (2013). Infective endocarditis caused by Granulicatella adiacens. Indian Heart Journal, 65(4), 447-449.Cargill, J. S., Scott, K. S., Gascoyne-Binzi, D., & Sandoe, J. A. T. (2012). Granulicatella infection: Diagnosis and management. Journal of Medical Microbiology, 61(6), 755-761.Turnbull, P. C. B. (1996). Bacillus. In S. Baron (Ed.), Medical microbiology (4th ed.). Galveston, TX: University of Texas Medical Branch. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/21413260Pan, J., Zhao, J., & Jiang, N. (2014). Oral cavity infection: An adverse effect after the treatment of oral cancer in aged individuals. Journal of Applied Oral Science, 22(4), 261-267.Gutta-perchaobturación del conducto radicularcontaminaciónbacteriaTG 2018 ODOGutta-percharoot canal obturationcontaminationbacteriaFacultative anaerobic bacteria on dentistry students’ gutta-percha points: The importance of disinfectionActa de memoriashttp://purl.org/coar/resource_type/c_f744http://purl.org/coar/resource_type/c_8042info:eu-repo/semantics/workingPaperinfo:eu-repo/semantics/acceptedVersionAtribucióninfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2PublicationORIGINAL2018_Facultative anaerobic bacteria on dentistry students Feb.pdf2018_Facultative anaerobic bacteria on dentistry students Feb.pdfArtículoapplication/pdf150917https://repository.ucc.edu.co/bitstreams/9fc6f0d0-972b-436a-a6cd-0e45e1630ec8/download7c0d49a3646806b0825c80a16f3a5535MD53Derecho repositorio Conos de gutapercha.pdfDerecho repositorio Conos de gutapercha.pdfLicencia de 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Facultative anaerobic bacteria on dentistry students’ gutta-percha points: The importance of disinfection

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