Effects of a functional food supplemented with probiotics on biological factors related to dental caries in children: a pilot study
Purpose The aim of this study was to evaluate the effects of a functional food supplemented with probiotics on biological factors related to dental caries in children aged 3–5 years. Methods A repeated measures pilot study was conducted with children who have consumed a commercial milk containing tw...
- Autores:
-
Angarita Díaz, María del Pilar
Angarita Díaz, María del Pilar
Forero Escobar, Diana
Cerón Bastidas, Ximena Andrea
Cisneros Hidalgo, Cristhian Ariel
Dávila Narváez, Fernando
Bedoya Correa, Claudia María
Da Cunha Freitas, Sidónio Ricardo
Cabrera Arango, Claudia Liliana
Melo Colina, Roxana
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2019
- Institución:
- Universidad Cooperativa de Colombia
- Repositorio:
- Repositorio UCC
- Idioma:
- OAI Identifier:
- oai:repository.ucc.edu.co:20.500.12494/16104
- Acceso en línea:
- https://hdl.handle.net/20.500.12494/16104
- Palabra clave:
- Alimentos funcional
Probioticos
Caries
Niñez
Functional food
Probiotics
Dental caries
Child welfare
- Rights
- openAccess
- License
- Atribución – No comercial – Sin Derivar
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Effects of a functional food supplemented with probiotics on biological factors related to dental caries in children: a pilot study |
| title |
Effects of a functional food supplemented with probiotics on biological factors related to dental caries in children: a pilot study |
| spellingShingle |
Effects of a functional food supplemented with probiotics on biological factors related to dental caries in children: a pilot study Alimentos funcional Probioticos Caries Niñez Functional food Probiotics Dental caries Child welfare |
| title_short |
Effects of a functional food supplemented with probiotics on biological factors related to dental caries in children: a pilot study |
| title_full |
Effects of a functional food supplemented with probiotics on biological factors related to dental caries in children: a pilot study |
| title_fullStr |
Effects of a functional food supplemented with probiotics on biological factors related to dental caries in children: a pilot study |
| title_full_unstemmed |
Effects of a functional food supplemented with probiotics on biological factors related to dental caries in children: a pilot study |
| title_sort |
Effects of a functional food supplemented with probiotics on biological factors related to dental caries in children: a pilot study |
| dc.creator.fl_str_mv |
Angarita Díaz, María del Pilar Angarita Díaz, María del Pilar Forero Escobar, Diana Cerón Bastidas, Ximena Andrea Cisneros Hidalgo, Cristhian Ariel Dávila Narváez, Fernando Bedoya Correa, Claudia María Da Cunha Freitas, Sidónio Ricardo Cabrera Arango, Claudia Liliana Melo Colina, Roxana |
| dc.contributor.advisor.none.fl_str_mv |
Lygidakis, Nick A. |
| dc.contributor.author.none.fl_str_mv |
Angarita Díaz, María del Pilar Angarita Díaz, María del Pilar Forero Escobar, Diana Cerón Bastidas, Ximena Andrea Cisneros Hidalgo, Cristhian Ariel Dávila Narváez, Fernando Bedoya Correa, Claudia María Da Cunha Freitas, Sidónio Ricardo Cabrera Arango, Claudia Liliana Melo Colina, Roxana |
| dc.subject.spa.fl_str_mv |
Alimentos funcional Probioticos Caries Niñez |
| topic |
Alimentos funcional Probioticos Caries Niñez Functional food Probiotics Dental caries Child welfare |
| dc.subject.other.spa.fl_str_mv |
Functional food Probiotics Dental caries Child welfare |
| description |
Purpose The aim of this study was to evaluate the effects of a functional food supplemented with probiotics on biological factors related to dental caries in children aged 3–5 years. Methods A repeated measures pilot study was conducted with children who have consumed a commercial milk containing two lactic acid bacteria as probiotics (WP milk) for a period of 3 months and another period of 3 months consuming a milk without probiotics (NP milk). Salivary pH, plaque index, pH variation before and after a sugar rinse, quantification of Streptococcus mutans in saliva and demineralisation of the carious lesions were determined at the beginning and at the end of both milk ingestion periods. Results Regarding WP milk, a non-significant decrease in terms of the concentration of S. mutans and pH variation (p > 0.05), a significant decrease (i.e. acidification) in salivary pH (p < 0.01) and a remineralisation of 39.4% of the caries were found. On the other hand, for NP milk, a non-significant increase in terms of the concentration of S. mutans, pH variation, salivary pH (p > 0.05) and a remineralisation of 64.2% were found. Conclusions Lactic acid probiotics can contribute to the decrease in the number of cariogenic microorganisms. However, the appropriate selection of the bacteria type with regard to its acidogenicity is fundamental to avoid the generation of an effect contrary to that expected, e.g. a significant decrease in salivary pH. |
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2019 |
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2019-08-06 |
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2020-01-20T16:22:22Z |
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2020-01-20T16:22:22Z |
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Artículo |
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1996-9805 |
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10.1007/s40368-019-00468-y. |
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Angarita-Díaz, M.P., Forero-Escobar, D., Cerón-Bastidas, X.A., Cisneros Hidalgo, C.A., Dávila Narvaez, F., Bedoya Correa, C.M., Freitas, S., Cabrera Arango , C.L. y Melo Colina, L. (2019). Effects of a functional food supplemented with probiotics on biological factors related to dental caries in children: a pilot study. Eur Arch Paediatr Dent. DOI: 10.1007/s40368-019-00468-y. Recuperado de: https://link.springer.com/article/10.1007%2Fs40368-019-00468-y |
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1996-9805 10.1007/s40368-019-00468-y. Angarita-Díaz, M.P., Forero-Escobar, D., Cerón-Bastidas, X.A., Cisneros Hidalgo, C.A., Dávila Narvaez, F., Bedoya Correa, C.M., Freitas, S., Cabrera Arango , C.L. y Melo Colina, L. (2019). Effects of a functional food supplemented with probiotics on biological factors related to dental caries in children: a pilot study. Eur Arch Paediatr Dent. DOI: 10.1007/s40368-019-00468-y. Recuperado de: https://link.springer.com/article/10.1007%2Fs40368-019-00468-y |
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https://link.springer.com/article/10.1007%2Fs40368-019-00468-y |
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European Archives of Paediatric Dentistry |
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Ahola AJ, Yli-Knuuttila H, Suomalainen T, et al. Short-term consumption of probiotic-containing cheese and its effect on dental caries risk factors. Arch Oral Biol. 2002;47:799–804. Alanzi A, Honkala S, Honkala E, et al. Effect of Lactobacillus rhamnosus and Bifidobacterium lactis on gingival health, dental plaque, and periodontopathogens in adolescents: a randomised placebo-controlled clinical trial. Benef Microbes. 2018;9:593–602. American Academy of Pediatric Dentistry. Policy on early childhood caries (ECC): classifications, consequences, and preventive strategies. Pediatr Dent. 2016;6:52–4. Angarita MP, Freitas S, Mira A, et al. Inhibition activity of probiotic supernatants against the cariogenic Streptococcus mutans. J Ecosyst Ecography. 2017;7:2. Castilho LS, Cotta FV, Bueno AC, et al. Validation of DIAGNOdent laser fluorescence and the International Caries Detection and Assessment System (ICDAS) in diagnosis of occlusal caries in permanent teeth: an in vivo study. Eur J Oral Sci. 2016;124:188–94. Coqueiro AY, Bonvini A, Raizel R, et al. Probiotic supplementation in dental caries: is it possible to replace conventional treatment? Nutrire. 2018;43:6. Cunha-Cruz J, Scott J, Rothen M, et al. Salivary characteristics and dental caries: evidence from general dental practices. J Am Dent Assoc. 2013;144:e31–40. De Keersmaecker SC, Verhoeven TL, Desair J, et al. Strong antimicrobial activity of Lactobacillus rhamnosus GG against Salmonella typhimurium is due to accumulation of lactic acid. FEMS Microbiol Lett. 2006;259:89–96. Fejerskov O, Edwina AM. Dental caries: the disease and its clinical management. Oxford: Blackwell Munksgaard; 2008. Haukioja A, Söderling E, Tenovuo J. Acid production from sugars and sugar alcohols by probiotic lactobacilli and bifidobacteria in vitro. Caries Res. 2008;42:449–53. Hicks J, Garcia-Godoy F, Flaitz C. Biological factors in dental caries: role of saliva and dental plaque in the dynamic process of demineralization and remineralization (part 1). J Clin Pediatr Dent. 2003;28:47–52. Jiang Q, Kainulainen V, Stamatova I, et al. Lactobacillus rhamnosus GG in experimental oral biofilms exposed to different carbohydrate sources. Caries Res. 2018;52:220–9. Jindal G, Pandey RK, Agarwal J, et al. A comparative evaluation of probiotics on salivary mutans streptococci counts in Indian children. Eur Arch Paediatr Dent. 2011;12:211–5. Joint FAO/WHO Working Group Report on Drafting Guidelines for the Evaluation of Probiotics in Food. London, Ontario, Canada. April 30 and May 1, 2002. Joshi A, Suja S, Jashbhai P. Identification of Lactobacillus rhamnosus GG bacteriocin gene determinants expressed in vivo in murine gut. World J Dairy Food Sci. 2014;9:70–8. Lin YT, Chou CC, Hsu CY. Effects of Lactobacillus casei Shirota intake on caries risk in children. J Dent Sci. 2017;12:179–84. Marsh PD. In sickness and in health-what does the oral microbiome mean to us? An ecological perspective. Adv Dent Res. 2018;29:60–5. Martignon S, Bautista-Mendoza G, González-Carrera MC, et al. Instruments for evaluating oral health knowledge, attitudes and practice for parents/caregivers of small children. Rev salud pública. 2008;10:308–14 Ministry of Health of Colombia. 2014. IV National Oral Health Sttudy ENSAB IV. Bogotá: Minsalud. 2014. https://www.minsalud.gov.co/sites/rid/Lists/BibliotecaDigital/RIDE/VS/PP/ENSAB-IV-Situacion-Bucal-Actual.pdf. Accessed 28 Sept 2018. Mira A. Oral Microbiome studies: potential diagnostic and therapeutic implications. Adv Dent Res. 2018;29:71–7. Mombelli A, Van Oosten MAC, Schürch E, et al. The microbiota associated with successful or failing osseointegrated titanium implants. Oral Microbiol Immunol. 1987;2:145–51. Nadelman P, Magno MB, Masterson D, et al. Are dairy products containing probiotics beneficial for oral health? A systematic review and meta-analysis. Clin Oral Investig. 2018;22:2763–85. Näse L, Hatakka K, Savilahti E, et al. Effect of long-term consumption of a probiotic bacterium, Lactobacillus rhamnosus GG, in milk on dental caries and caries risk in children. Caries Res. 2001;35:412–20. Pham LC, van Spanning RJ, Röling WF, et al. Effects of probiotic Lactobacillus salivarius W24 on the compositional stability of oral microbial communities. Arch Oral Microbiol. 2009;54:132–7. Pham LC, Hoogenkamp MA, Exterkate RA, et al. Effects of Lactobacillus rhamnosus GG on saliva-derived microcosms. Arch Oral Biol. 2011;56:136–47. Rojas EU. Viability and variability assessment of salivary microbiota stored at different temperatures. Porto Alegre: Universidade Federal Do Rio Grande Do Soul. Brasil; 2007. https ://lume.ufrgs .br/ handl e/10183 /12140 . Accessed 10 July 2018. Schwendicke F, Dörfer C, Kneist S, Meyer-Lueckel H, et al. Cariogenic effects of probiotic Lactobacillus rhamnosus GG in a dental biofilm model. Caries Res. 2014;48:186–92. Seminario-Amez M, López-López J, Estrugo-Devesa A, et al. Probiotics and oral health: a systematic review. Med Oral Patol Oral Cir Bucal. 2017;22:282–8. Simón-Soro A, Mira A. Solving the etiology of dental caries. Trends Microbiol. 2015;23:76–82 Söderling E, Marttinen A, Haukioja A. Probiotic lactobacilli interfere with Streptococcus mutans biofilm formation in vitro. Curr Microbiol. 2011;62:618–22. Vikneshan M, Ankola AV, Hiremath A, et al. Functional foods and its role in improving oral health. J Sci Dent. 2016;6:13–9. Villavicencio J, Villegas LM, Arango MC, et al. Effects of a food enriched with probiotics on Streptococcus mutans and Lactobacillus spp. salivary counts in preschool children: a cluster randomized trial. J Appl Oral Sci. 2018;26:e20170318 WHO. WHO Expert Consultation on Public Health Intervention against Early Childhood Caries: report of a meeting. Geneva: World Health Organization; 2017. https ://apps.who.int/iris/handl e/10665 /25562 7. |
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Universidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Programa de Odontología, Villavicencio, Colombia, 00000 Nick A. Lygidakis |
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Lygidakis, Nick A.Angarita Díaz, María del PilarAngarita Díaz, María del PilarForero Escobar, DianaCerón Bastidas, Ximena AndreaCisneros Hidalgo, Cristhian ArielDávila Narváez, FernandoBedoya Correa, Claudia MaríaDa Cunha Freitas, Sidónio RicardoCabrera Arango, Claudia LilianaMelo Colina, Roxana2020-01-20T16:22:22Z2020-01-20T16:22:22Z2019-08-061996-980510.1007/s40368-019-00468-y.https://hdl.handle.net/20.500.12494/16104Angarita-Díaz, M.P., Forero-Escobar, D., Cerón-Bastidas, X.A., Cisneros Hidalgo, C.A., Dávila Narvaez, F., Bedoya Correa, C.M., Freitas, S., Cabrera Arango , C.L. y Melo Colina, L. (2019). Effects of a functional food supplemented with probiotics on biological factors related to dental caries in children: a pilot study. Eur Arch Paediatr Dent. DOI: 10.1007/s40368-019-00468-y. Recuperado de: https://link.springer.com/article/10.1007%2Fs40368-019-00468-yPurpose The aim of this study was to evaluate the effects of a functional food supplemented with probiotics on biological factors related to dental caries in children aged 3–5 years. Methods A repeated measures pilot study was conducted with children who have consumed a commercial milk containing two lactic acid bacteria as probiotics (WP milk) for a period of 3 months and another period of 3 months consuming a milk without probiotics (NP milk). Salivary pH, plaque index, pH variation before and after a sugar rinse, quantification of Streptococcus mutans in saliva and demineralisation of the carious lesions were determined at the beginning and at the end of both milk ingestion periods. Results Regarding WP milk, a non-significant decrease in terms of the concentration of S. mutans and pH variation (p > 0.05), a significant decrease (i.e. acidification) in salivary pH (p < 0.01) and a remineralisation of 39.4% of the caries were found. On the other hand, for NP milk, a non-significant increase in terms of the concentration of S. mutans, pH variation, salivary pH (p > 0.05) and a remineralisation of 64.2% were found. Conclusions Lactic acid probiotics can contribute to the decrease in the number of cariogenic microorganisms. However, the appropriate selection of the bacteria type with regard to its acidogenicity is fundamental to avoid the generation of an effect contrary to that expected, e.g. a significant decrease in salivary pH.http://scienti.colciencias.gov.co:8081/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0001561382https://orcid.org/0000-0002-5435-3456GIOMETGIOMGIODmaria.angaritad@campusucc.edu.codiana.foreroe@ucc.edu.coximena.ceron@campusucc.edu.cocristhian.cisneros@campusucc.edu.cofernando.davila@ucc.edu.coclaudia.bedoyac@campusucc.edu.cosidfrei@gmail.comclaudia.cabrera@campusucc.edu.coroxana.melo@campusucc.edu.co9Universidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Programa de Odontología, Villavicencio, Colombia, 00000Nick A. LygidakisOdontologíaVillavicenciohttps://link.springer.com/article/10.1007%2Fs40368-019-00468-yEuropean Archives of Paediatric DentistryAhola AJ, Yli-Knuuttila H, Suomalainen T, et al. Short-term consumption of probiotic-containing cheese and its effect on dental caries risk factors. Arch Oral Biol. 2002;47:799–804.Alanzi A, Honkala S, Honkala E, et al. Effect of Lactobacillus rhamnosus and Bifidobacterium lactis on gingival health, dental plaque, and periodontopathogens in adolescents: a randomised placebo-controlled clinical trial. Benef Microbes. 2018;9:593–602.American Academy of Pediatric Dentistry. Policy on early childhood caries (ECC): classifications, consequences, and preventive strategies. Pediatr Dent. 2016;6:52–4.Angarita MP, Freitas S, Mira A, et al. Inhibition activity of probiotic supernatants against the cariogenic Streptococcus mutans. J Ecosyst Ecography. 2017;7:2.Castilho LS, Cotta FV, Bueno AC, et al. Validation of DIAGNOdent laser fluorescence and the International Caries Detection and Assessment System (ICDAS) in diagnosis of occlusal caries in permanent teeth: an in vivo study. Eur J Oral Sci. 2016;124:188–94.Coqueiro AY, Bonvini A, Raizel R, et al. Probiotic supplementation in dental caries: is it possible to replace conventional treatment? Nutrire. 2018;43:6.Cunha-Cruz J, Scott J, Rothen M, et al. Salivary characteristics and dental caries: evidence from general dental practices. J Am Dent Assoc. 2013;144:e31–40.De Keersmaecker SC, Verhoeven TL, Desair J, et al. Strong antimicrobial activity of Lactobacillus rhamnosus GG against Salmonella typhimurium is due to accumulation of lactic acid. FEMS Microbiol Lett. 2006;259:89–96.Fejerskov O, Edwina AM. Dental caries: the disease and its clinical management. Oxford: Blackwell Munksgaard; 2008.Haukioja A, Söderling E, Tenovuo J. Acid production from sugars and sugar alcohols by probiotic lactobacilli and bifidobacteria in vitro. Caries Res. 2008;42:449–53.Hicks J, Garcia-Godoy F, Flaitz C. Biological factors in dental caries: role of saliva and dental plaque in the dynamic process of demineralization and remineralization (part 1). J Clin Pediatr Dent. 2003;28:47–52.Jiang Q, Kainulainen V, Stamatova I, et al. Lactobacillus rhamnosus GG in experimental oral biofilms exposed to different carbohydrate sources. Caries Res. 2018;52:220–9.Jindal G, Pandey RK, Agarwal J, et al. A comparative evaluation of probiotics on salivary mutans streptococci counts in Indian children. Eur Arch Paediatr Dent. 2011;12:211–5.Joint FAO/WHO Working Group Report on Drafting Guidelines for the Evaluation of Probiotics in Food. London, Ontario, Canada. April 30 and May 1, 2002.Joshi A, Suja S, Jashbhai P. Identification of Lactobacillus rhamnosus GG bacteriocin gene determinants expressed in vivo in murine gut. World J Dairy Food Sci. 2014;9:70–8.Lin YT, Chou CC, Hsu CY. Effects of Lactobacillus casei Shirota intake on caries risk in children. J Dent Sci. 2017;12:179–84.Marsh PD. In sickness and in health-what does the oral microbiome mean to us? An ecological perspective. Adv Dent Res. 2018;29:60–5.Martignon S, Bautista-Mendoza G, González-Carrera MC, et al. Instruments for evaluating oral health knowledge, attitudes and practice for parents/caregivers of small children. Rev salud pública. 2008;10:308–14Ministry of Health of Colombia. 2014. IV National Oral Health Sttudy ENSAB IV. Bogotá: Minsalud. 2014. https://www.minsalud.gov.co/sites/rid/Lists/BibliotecaDigital/RIDE/VS/PP/ENSAB-IV-Situacion-Bucal-Actual.pdf. Accessed 28 Sept 2018.Mira A. Oral Microbiome studies: potential diagnostic and therapeutic implications. Adv Dent Res. 2018;29:71–7.Mombelli A, Van Oosten MAC, Schürch E, et al. The microbiota associated with successful or failing osseointegrated titanium implants. Oral Microbiol Immunol. 1987;2:145–51.Nadelman P, Magno MB, Masterson D, et al. Are dairy products containing probiotics beneficial for oral health? A systematic review and meta-analysis. Clin Oral Investig. 2018;22:2763–85.Näse L, Hatakka K, Savilahti E, et al. Effect of long-term consumption of a probiotic bacterium, Lactobacillus rhamnosus GG, in milk on dental caries and caries risk in children. Caries Res. 2001;35:412–20.Pham LC, van Spanning RJ, Röling WF, et al. Effects of probiotic Lactobacillus salivarius W24 on the compositional stability of oral microbial communities. Arch Oral Microbiol. 2009;54:132–7.Pham LC, Hoogenkamp MA, Exterkate RA, et al. Effects of Lactobacillus rhamnosus GG on saliva-derived microcosms. Arch Oral Biol. 2011;56:136–47.Rojas EU. Viability and variability assessment of salivary microbiota stored at different temperatures. Porto Alegre: Universidade Federal Do Rio Grande Do Soul. Brasil; 2007. https ://lume.ufrgs .br/ handl e/10183 /12140 . Accessed 10 July 2018.Schwendicke F, Dörfer C, Kneist S, Meyer-Lueckel H, et al. Cariogenic effects of probiotic Lactobacillus rhamnosus GG in a dental biofilm model. Caries Res. 2014;48:186–92.Seminario-Amez M, López-López J, Estrugo-Devesa A, et al. Probiotics and oral health: a systematic review. Med Oral Patol Oral Cir Bucal. 2017;22:282–8.Simón-Soro A, Mira A. Solving the etiology of dental caries. Trends Microbiol. 2015;23:76–82Söderling E, Marttinen A, Haukioja A. Probiotic lactobacilli interfere with Streptococcus mutans biofilm formation in vitro. Curr Microbiol. 2011;62:618–22.Vikneshan M, Ankola AV, Hiremath A, et al. Functional foods and its role in improving oral health. J Sci Dent. 2016;6:13–9.Villavicencio J, Villegas LM, Arango MC, et al. Effects of a food enriched with probiotics on Streptococcus mutans and Lactobacillus spp. salivary counts in preschool children: a cluster randomized trial. J Appl Oral Sci. 2018;26:e20170318WHO. WHO Expert Consultation on Public Health Intervention against Early Childhood Caries: report of a meeting. Geneva: World Health Organization; 2017. https ://apps.who.int/iris/handl e/10665 /25562 7.Alimentos funcionalProbioticosCariesNiñezFunctional foodProbioticsDental cariesChild welfareEffects of a functional food supplemented with probiotics on biological factors related to dental caries in children: a pilot studyArtículohttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAtribución – No comercial – Sin Derivarinfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2PublicationORIGINALEffect of probiotic.pdfEffect of probiotic.pdfArtículoapplication/pdf465904https://repository.ucc.edu.co/bitstreams/539d5ec7-779c-41f9-b7cf-c75b8b59c0b5/download0489b1b2728c411ba26863de33e12fd2MD51Licencia de uso RI Ver3 (1).pdfLicencia de uso RI Ver3 (1).pdfLicencia de 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