Effects of Line and Pillar Array Microengineered SiO2 Thin Films on the Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells

A primary goal in bone tissue engineering is the design of implants that induce controlled, guided, and rapid healing. The events that normally lead to the integration of an implant into bone and determine the performance of the device occur mainly at the tissue−implant interface. Topographical surf...

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Autores:
Peláez Vargas, Alejandro
Carvalho, Ángela
Hansford, Derek
Fernandes, Maria Helena
Monteiro, Fernando Jorge
Tipo de recurso:
Article of journal
Fecha de publicación:
2016
Institución:
Universidad Cooperativa de Colombia
Repositorio:
Repositorio UCC
Idioma:
OAI Identifier:
oai:repository.ucc.edu.co:20.500.12494/1186
Acceso en línea:
https://hdl.handle.net/20.500.12494/1186
Palabra clave:
Micropatrones
Biomateriales Dentales
Rights
closedAccess
License
Licencia CC
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oai_identifier_str oai:repository.ucc.edu.co:20.500.12494/1186
network_acronym_str COOPER2
network_name_str Repositorio UCC
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dc.title.spa.fl_str_mv Effects of Line and Pillar Array Microengineered SiO2 Thin Films on the Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells
title Effects of Line and Pillar Array Microengineered SiO2 Thin Films on the Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells
spellingShingle Effects of Line and Pillar Array Microengineered SiO2 Thin Films on the Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells
Micropatrones
Biomateriales Dentales
title_short Effects of Line and Pillar Array Microengineered SiO2 Thin Films on the Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells
title_full Effects of Line and Pillar Array Microengineered SiO2 Thin Films on the Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells
title_fullStr Effects of Line and Pillar Array Microengineered SiO2 Thin Films on the Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells
title_full_unstemmed Effects of Line and Pillar Array Microengineered SiO2 Thin Films on the Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells
title_sort Effects of Line and Pillar Array Microengineered SiO2 Thin Films on the Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells
dc.creator.fl_str_mv Peláez Vargas, Alejandro
Carvalho, Ángela
Hansford, Derek
Fernandes, Maria Helena
Monteiro, Fernando Jorge
dc.contributor.author.none.fl_str_mv Peláez Vargas, Alejandro
Carvalho, Ángela
Hansford, Derek
Fernandes, Maria Helena
Monteiro, Fernando Jorge
dc.subject.spa.fl_str_mv Micropatrones
Biomateriales Dentales
topic Micropatrones
Biomateriales Dentales
description A primary goal in bone tissue engineering is the design of implants that induce controlled, guided, and rapid healing. The events that normally lead to the integration of an implant into bone and determine the performance of the device occur mainly at the tissue−implant interface. Topographical surface modification of a biomaterial might be an efficient tool for inducing stem cell osteogenic differentiation and replace the use of biochemical stimuli. The main goal of this work was to develop micropatterned bioactive silica thin films to induce the osteogenic differentiation of human bone marrow-derived mesenchymal stem cells (hMSCs) only through topographical stimuli. Line and pillar micropatterns were developed by a combination of sol−gel/soft lithography and characterized by scanning electron microscopy, atomic force microscopy, and contact angle measurements. hMSCs were cultured onto the microfabricated thin films and flat control for up to 21 days under basal conditions. The micropatterned groups induced levels of osteogenic differentiation and expression of osteoblast-associated markers higher than those of the flat controls. Via comparison of the micropatterns, the pillars caused a stronger response of the osteogenic differentiation of hMSCs with a higher level of expression of osteoblast-associated markers, ALP activity, and extracellular matrix mineralization after the cells had been cultured for 21 days. These findings suggest that specific microtopographic cues can direct hMSCs toward osteogenic differentiation.
publishDate 2016
dc.date.available.none.fl_str_mv 2016
2017-08-18T23:34:29Z
dc.date.issued.none.fl_str_mv 2016
dc.date.accessioned.none.fl_str_mv 2017-08-18T23:34:29Z
dc.type.none.fl_str_mv Artículo
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dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/20.500.12494/1186
dc.identifier.bibliographicCitation.spa.fl_str_mv Peláez Vargas, A., Carvalho, A., Hansford, D., Fernandes, M, Monteiro, F. J. (2014). Effects of Line and Pillar Array Microengineered SiO2 Thin Films on the Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells. Langmuir, 32, 1091-1100. doi: 10.1021/acs.langmuir.5b03955
url https://hdl.handle.net/20.500.12494/1186
identifier_str_mv Peláez Vargas, A., Carvalho, A., Hansford, D., Fernandes, M, Monteiro, F. J. (2014). Effects of Line and Pillar Array Microengineered SiO2 Thin Films on the Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells. Langmuir, 32, 1091-1100. doi: 10.1021/acs.langmuir.5b03955
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rights_invalid_str_mv Licencia CC
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eu_rights_str_mv closedAccess
dc.publisher.spa.fl_str_mv Universidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Programa de Odontología, Medellín y Envigado, Colombia, 00000
dc.publisher.program.spa.fl_str_mv Odontología
dc.publisher.place.spa.fl_str_mv Medellín
institution Universidad Cooperativa de Colombia
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spelling Peláez Vargas, AlejandroCarvalho, ÁngelaHansford, DerekFernandes, Maria HelenaMonteiro, Fernando Jorge2017-08-18T23:34:29Z20162017-08-18T23:34:29Z2016https://hdl.handle.net/20.500.12494/1186Peláez Vargas, A., Carvalho, A., Hansford, D., Fernandes, M, Monteiro, F. J. (2014). Effects of Line and Pillar Array Microengineered SiO2 Thin Films on the Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells. Langmuir, 32, 1091-1100. doi: 10.1021/acs.langmuir.5b03955A primary goal in bone tissue engineering is the design of implants that induce controlled, guided, and rapid healing. The events that normally lead to the integration of an implant into bone and determine the performance of the device occur mainly at the tissue−implant interface. Topographical surface modification of a biomaterial might be an efficient tool for inducing stem cell osteogenic differentiation and replace the use of biochemical stimuli. The main goal of this work was to develop micropatterned bioactive silica thin films to induce the osteogenic differentiation of human bone marrow-derived mesenchymal stem cells (hMSCs) only through topographical stimuli. Line and pillar micropatterns were developed by a combination of sol−gel/soft lithography and characterized by scanning electron microscopy, atomic force microscopy, and contact angle measurements. hMSCs were cultured onto the microfabricated thin films and flat control for up to 21 days under basal conditions. The micropatterned groups induced levels of osteogenic differentiation and expression of osteoblast-associated markers higher than those of the flat controls. Via comparison of the micropatterns, the pillars caused a stronger response of the osteogenic differentiation of hMSCs with a higher level of expression of osteoblast-associated markers, ALP activity, and extracellular matrix mineralization after the cells had been cultured for 21 days. These findings suggest that specific microtopographic cues can direct hMSCs toward osteogenic differentiation.alejandro.pelaezv@campusucc.edu.coUniversidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Programa de Odontología, Medellín y Envigado, Colombia, 00000OdontologíaMedellínMicropatronesBiomateriales DentalesEffects of Line and Pillar Array Microengineered SiO2 Thin Films on the Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem CellsArtículohttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionLicencia CCinfo:eu-repo/semantics/closedAccesshttp://purl.org/coar/access_right/c_14cbPublicationLICENSElicense.txtlicense.txttext/plain; charset=utf-84553https://repository.ucc.edu.co/bitstreams/f40ab221-c900-41db-bf7a-f71de2f05140/downloadcb8f4d9b72d863ad6737f22af442cc82MD52TEXT2016 - other pelaez-vargas 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