Detection of submicroscopic infection with Plasmodium spp., using classical and molecular techniques in pregnant patients from Córdoba, Colombia
Introduction: Gestational malaria affects both the mother and the development of her embryo or fetus. Rapid diagnosis and timely and effective treatment are required to prevent complications and deaths. Objective: To compare thick blood smear with nested PCR and real-time PCR (qRT-PCR) for the diagn...
- Autores:
-
Blanquiceth Y.P.
Murillo Gomez, Oscar
Maestre A.E.
Corredor M.
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2014
- Institución:
- Universidad Cooperativa de Colombia
- Repositorio:
- Repositorio UCC
- Idioma:
- OAI Identifier:
- oai:repository.ucc.edu.co:20.500.12494/42688
- Acceso en línea:
- https://www.scopus.com/inward/record.uri?eid=2-s2.0-84903203158&partnerID=40&md5=04abf1a520b9ab82b4fc008d5ac4eec6
https://hdl.handle.net/20.500.12494/42688
- Palabra clave:
- article
blood
blood smear
clinical article
Colombia
diagnostic test accuracy study
DNA extraction
female
gestational malaria
human
malaria
microorganism detection
molecular diagnosis
nested polymerase chain reaction
nonhuman
placenta blood
Plasmodium falciparum
Plasmodium vivax
polymerase chain reaction
pregnant woman
real time polymerase chain reaction
sensitivity and specificity
umbilical cord blood
- Rights
- closedAccess
- License
- http://purl.org/coar/access_right/c_14cb
| Summary: | Introduction: Gestational malaria affects both the mother and the development of her embryo or fetus. Rapid diagnosis and timely and effective treatment are required to prevent complications and deaths. Objective: To compare thick blood smear with nested PCR and real-time PCR (qRT-PCR) for the diagnosis of submicroscopic infections with Plasmodium falciparum and P. vivax. Methodology: 21 women with clinical manifestations of malaria, including both pregnant and non-pregnant, were studied in Puerto Libertador, Córdoba, Colombia. Peripheral blood specimens were obtained from all of them; umbilical cord and placenta blood specimens were taken in the pregnant ones. DNA was extracted and amplified for nested PCR or qRT-PCR. Statistical analysis was done using Graphpad PRISM and EPIDAT softwares. Results: The three techniques were satisfactory for the detection of Plasmodium falciparum and P. vivax in peripheral blood and in the umbilical cord and placenta specimens. Molecular tests were 100% sensitive and specific. Two submicroscopic cases of P. falciparum infection were detected with the two PCR techniques. Conclusion: qRT-PCR is advantageous over nested PCR because its standardization is shorter, it requires lesser infrastructure and it allows the quantification of DNA. |
|---|