Molecular and serological detection of Ehrlichia canis and Babesia vogeli in dogs in Colombia

Ehrlichiosis and babesiosis are tick-borne diseases, caused mainly by Ehrlichia canis and Babesia canis, respectively, with a worldwide occurrence in dogs, whose main vector is the brown-dog tick, Rhipicephalus sanguineus. The present work aimed to detect the presence of E. canis and Babesia sp. in...

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Autores:: Vargas-Hernández G.
André M.R.
Faria J.L.M.
Munhoz T.D.
Hernandez Rodriguez, Milena Esney
Machado R.Z.
Tinucci-Costa M.

Tipo de recurso:: Article of journal

Fecha de publicación:: 2012

Institución:: Universidad Cooperativa de Colombia

Repositorio:: Repositorio UCC

Idioma:

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network_acronym_str	COOPER2
network_name_str	Repositorio UCC
repository_id_str
spelling	Vargas-Hernández G.André M.R.Faria J.L.M.Munhoz T.D.Hernandez Rodriguez, Milena EsneyMachado R.Z.Tinucci-Costa M.2021-12-16T22:15:39Z2021-12-16T22:15:39Z2012https://doi.org/10.1159/000371765https://psicologiaysalud.uv.mx/index.php/psicysalud/index03044017https://hdl.handle.net/20.500.12494/41616Vargas G,André MR,Faria JLM,Munhoz TD,Hernandez M,Machado RZ,Tinucci M. Molecular and serological detection of Ehrlichia canis and Babesia vogeli in dogs in Colombia. VET PARASITOL. 2012. 186. (3-4):p. 254-260. .Ehrlichiosis and babesiosis are tick-borne diseases, caused mainly by Ehrlichia canis and Babesia canis, respectively, with a worldwide occurrence in dogs, whose main vector is the brown-dog tick, Rhipicephalus sanguineus. The present work aimed to detect the presence of E. canis and Babesia sp. in 91 dog blood samples in Colombia, by molecular and serological techniques. We also performed sequence alignment to indicate the identity of the parasite species infecting these animals. The present work shows the first molecular detection of E. canis and B. vogeli in dogs from Colombia. Immunoglobulin-G (IgG) antibodies to E. canis and Babesia vogeli were found in 75 (82.4%) and 47 (51.6%) sampled dogs, respectively. Thirty-seven (40.6%) and 5 (5.5%) dogs were positive in PCR for E. canis and Babesia sp., respectively. After sequencing, amplicons showed 99% of identity with isolates of E. canis and B. vogeli. The phylogenetic trees based on 16S rRNA-Anaplasmataceae sequences and 18S rRNA-piroplasmid sequences supported the identity of the found E. canis and B. vogeli DNAs, respectively. The present work shows the first molecular detection of E. canis and B. vogeli in dogs in Colombia. © 2011 Elsevier B.V.milena.hernandez@campusucc.edu.co260-254Elsevier BVimmunoglobulin GRNA 16Santibody detectionarticleBabesiaBabesia vogeliblood samplingColombiacontrolled studydogEhrlichia canisgeographic distributionnonhumannucleotide sequenceparasite identificationphylogenetic treepolymerase chain reactionRNA sequencesequence alignmentsequence analysisserologyAnimalsBabesiaBabesiosisColombiaDog DiseasesDogsEhrlichia canisEhrlichiosisPhylogenySeroepidemiologic StudiesAnaplasmataceaeAnimaliaBabesiaBabesia canisBabesia spCanis familiarisEhrlichia canisIxodidaPiroplasmidaRhipicephalus sanguineusMolecular and serological detection of Ehrlichia canis and Babesia vogeli in dogs in ColombiaArtículohttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionVET PARASITOLinfo:eu-repo/semantics/closedAccesshttp://purl.org/coar/access_right/c_14cbPublication20.500.12494/41616oai:repository.ucc.edu.co:20.500.12494/416162024-08-20 16:10:31.659metadata.onlyhttps://repository.ucc.edu.coRepositorio Institucional Universidad Cooperativa de Colombiabdigital@metabiblioteca.com
dc.title.spa.fl_str_mv	Molecular and serological detection of Ehrlichia canis and Babesia vogeli in dogs in Colombia
title	Molecular and serological detection of Ehrlichia canis and Babesia vogeli in dogs in Colombia
spellingShingle	Molecular and serological detection of Ehrlichia canis and Babesia vogeli in dogs in Colombia immunoglobulin G RNA 16S antibody detection article Babesia Babesia vogeli blood sampling Colombia controlled study dog Ehrlichia canis geographic distribution nonhuman nucleotide sequence parasite identification phylogenetic tree polymerase chain reaction RNA sequence sequence alignment sequence analysis serology Animals Babesia Babesiosis Colombia Dog Diseases Dogs Ehrlichia canis Ehrlichiosis Phylogeny Seroepidemiologic Studies Anaplasmataceae Animalia Babesia Babesia canis Babesia sp Canis familiaris Ehrlichia canis Ixodida Piroplasmida Rhipicephalus sanguineus
title_short	Molecular and serological detection of Ehrlichia canis and Babesia vogeli in dogs in Colombia
title_full	Molecular and serological detection of Ehrlichia canis and Babesia vogeli in dogs in Colombia
title_fullStr	Molecular and serological detection of Ehrlichia canis and Babesia vogeli in dogs in Colombia
title_full_unstemmed	Molecular and serological detection of Ehrlichia canis and Babesia vogeli in dogs in Colombia
title_sort	Molecular and serological detection of Ehrlichia canis and Babesia vogeli in dogs in Colombia
dc.creator.fl_str_mv	Vargas-Hernández G. André M.R. Faria J.L.M. Munhoz T.D. Hernandez Rodriguez, Milena Esney Machado R.Z. Tinucci-Costa M.
dc.contributor.author.none.fl_str_mv	Vargas-Hernández G. André M.R. Faria J.L.M. Munhoz T.D. Hernandez Rodriguez, Milena Esney Machado R.Z. Tinucci-Costa M.
dc.subject.spa.fl_str_mv	immunoglobulin G RNA 16S antibody detection article Babesia Babesia vogeli blood sampling Colombia controlled study dog Ehrlichia canis geographic distribution nonhuman nucleotide sequence parasite identification phylogenetic tree polymerase chain reaction RNA sequence sequence alignment sequence analysis serology Animals Babesia Babesiosis Colombia Dog Diseases Dogs Ehrlichia canis Ehrlichiosis Phylogeny Seroepidemiologic Studies Anaplasmataceae Animalia Babesia Babesia canis Babesia sp Canis familiaris Ehrlichia canis Ixodida Piroplasmida Rhipicephalus sanguineus
topic	immunoglobulin G RNA 16S antibody detection article Babesia Babesia vogeli blood sampling Colombia controlled study dog Ehrlichia canis geographic distribution nonhuman nucleotide sequence parasite identification phylogenetic tree polymerase chain reaction RNA sequence sequence alignment sequence analysis serology Animals Babesia Babesiosis Colombia Dog Diseases Dogs Ehrlichia canis Ehrlichiosis Phylogeny Seroepidemiologic Studies Anaplasmataceae Animalia Babesia Babesia canis Babesia sp Canis familiaris Ehrlichia canis Ixodida Piroplasmida Rhipicephalus sanguineus
description	Ehrlichiosis and babesiosis are tick-borne diseases, caused mainly by Ehrlichia canis and Babesia canis, respectively, with a worldwide occurrence in dogs, whose main vector is the brown-dog tick, Rhipicephalus sanguineus. The present work aimed to detect the presence of E. canis and Babesia sp. in 91 dog blood samples in Colombia, by molecular and serological techniques. We also performed sequence alignment to indicate the identity of the parasite species infecting these animals. The present work shows the first molecular detection of E. canis and B. vogeli in dogs from Colombia. Immunoglobulin-G (IgG) antibodies to E. canis and Babesia vogeli were found in 75 (82.4%) and 47 (51.6%) sampled dogs, respectively. Thirty-seven (40.6%) and 5 (5.5%) dogs were positive in PCR for E. canis and Babesia sp., respectively. After sequencing, amplicons showed 99% of identity with isolates of E. canis and B. vogeli. The phylogenetic trees based on 16S rRNA-Anaplasmataceae sequences and 18S rRNA-piroplasmid sequences supported the identity of the found E. canis and B. vogeli DNAs, respectively. The present work shows the first molecular detection of E. canis and B. vogeli in dogs in Colombia. © 2011 Elsevier B.V.
publishDate	2012
dc.date.issued.none.fl_str_mv	2012
dc.date.accessioned.none.fl_str_mv	2021-12-16T22:15:39Z
dc.date.available.none.fl_str_mv	2021-12-16T22:15:39Z
dc.type.none.fl_str_mv	Artículo
dc.type.coar.fl_str_mv	http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.coar.none.fl_str_mv	http://purl.org/coar/resource_type/c_6501
dc.type.coarversion.none.fl_str_mv	http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.driver.none.fl_str_mv	info:eu-repo/semantics/article
dc.type.redcol.none.fl_str_mv	http://purl.org/redcol/resource_type/ART
dc.type.version.none.fl_str_mv	info:eu-repo/semantics/publishedVersion
format	http://purl.org/coar/resource_type/c_6501
status_str	publishedVersion
dc.identifier.none.fl_str_mv	https://doi.org/10.1159/000371765 https://psicologiaysalud.uv.mx/index.php/psicysalud/index
dc.identifier.issn.spa.fl_str_mv	03044017
dc.identifier.uri.none.fl_str_mv	https://hdl.handle.net/20.500.12494/41616
dc.identifier.bibliographicCitation.spa.fl_str_mv	Vargas G,André MR,Faria JLM,Munhoz TD,Hernandez M,Machado RZ,Tinucci M. Molecular and serological detection of Ehrlichia canis and Babesia vogeli in dogs in Colombia. VET PARASITOL. 2012. 186. (3-4):p. 254-260. .
url	https://doi.org/10.1159/000371765 https://psicologiaysalud.uv.mx/index.php/psicysalud/index https://hdl.handle.net/20.500.12494/41616
identifier_str_mv	03044017 Vargas G,André MR,Faria JLM,Munhoz TD,Hernandez M,Machado RZ,Tinucci M. Molecular and serological detection of Ehrlichia canis and Babesia vogeli in dogs in Colombia. VET PARASITOL. 2012. 186. (3-4):p. 254-260. .
dc.relation.ispartofjournal.spa.fl_str_mv	VET PARASITOL
dc.rights.accessrights.none.fl_str_mv	info:eu-repo/semantics/closedAccess
dc.rights.coar.none.fl_str_mv	http://purl.org/coar/access_right/c_14cb
eu_rights_str_mv	closedAccess
rights_invalid_str_mv	http://purl.org/coar/access_right/c_14cb
dc.format.extent.spa.fl_str_mv	260-254
dc.publisher.spa.fl_str_mv	Elsevier BV
institution	Universidad Cooperativa de Colombia
repository.name.fl_str_mv	Repositorio Institucional Universidad Cooperativa de Colombia
repository.mail.fl_str_mv	bdigital@metabiblioteca.com
_version_	1814246518085910528

Molecular and serological detection of Ehrlichia canis and Babesia vogeli in dogs in Colombia

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