Molecular and in vitro characterization of field isolates of bovine herpesvirus-1.

Bovine Herpesvirus-1 (BoHV-1) is distributed worldwide and is a major pathogen in cattle, being the causal agent of a variety of clinical syndromes. The aim of this study was to isolate and to characterize (molecular and biological characterization) BoHV-1 from 29 immunosuppressed animals. It was po...

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Autores:
Ruiz Saenz, Julian
Jaime, Jairo
Ramirez, Gloria
Vera, Victor
Tipo de recurso:
Article of journal
Fecha de publicación:
2012
Institución:
Universidad Cooperativa de Colombia
Repositorio:
Repositorio UCC
Idioma:
OAI Identifier:
oai:repository.ucc.edu.co:20.500.12494/41401
Acceso en línea:
https://doi.org/10.1016/j.acci.2018.05.002
https://hdl.handle.net/20.500.12494/41401
Palabra clave:
Herpesvirus bovino 1
Infección genital Rinfección respiratoria
Infección latente
Rights
closedAccess
License
http://purl.org/coar/access_right/c_14cb
id COOPER2_e1ce10fdbcc4fe799230652977091650
oai_identifier_str oai:repository.ucc.edu.co:20.500.12494/41401
network_acronym_str COOPER2
network_name_str Repositorio UCC
repository_id_str
spelling Ruiz Saenz, JulianJaime, JairoRamirez, GloriaVera, Victor2021-12-16T22:15:29Z2021-12-16T22:15:29Z2012https://doi.org/10.1016/j.acci.2018.05.00216740769https://hdl.handle.net/20.500.12494/41401Ruiz J,Jaime J,Ramirez G,Vera V. Molecular and in vitro characterization of field isolates of bovine herpesvirus-1. Virol Sin. 2012. 27. (1):p. 26-37. .Bovine Herpesvirus-1 (BoHV-1) is distributed worldwide and is a major pathogen in cattle, being the causal agent of a variety of clinical syndromes. The aim of this study was to isolate and to characterize (molecular and biological characterization) BoHV-1 from 29 immunosuppressed animals. It was possible to obtain 18 isolates, each from a different animal, such as from the respiratory and reproductive tracts. In some cases the cytopathic effect was visible 12 hours post-inoculation, and became characteristic after 36-48 hours. Biological characteristics were evaluated and compared with Iowa and Colorado-1 reference strains, and differences were found in plaque size, virus titer measured by TCID50 and PFU/mL, and one step virus curves. These results showed that some isolates had a highly virulent-like behavior in vitro, compared to the reference strains, with shorter eclipse periods, faster release of virus into the supernatants, and higher burst size and viral titer. There were no differences in glycoprotein expression of BoHV-1 isolates, measured by Western blot on monolayers. Moreover, using restriction endonucleases analysis, most of the viruses were confirmed as BoHV-1.1 and just one of them was confirmed as BoHV-1.2a subtype. These findings suggest that some wild-type BoHV-1 isolates could be useful as seeds to develop new monovalent vaccines.0000-0002-1447-1458julian.ruizs@campusucc.edu.co37-26Ko hsueh chu pan sheHerpesvirus bovino 1Infección genital Rinfección respiratoriaInfección latenteMolecular and in vitro characterization of field isolates of bovine herpesvirus-1.Artículohttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionVirologica Sinicainfo:eu-repo/semantics/closedAccesshttp://purl.org/coar/access_right/c_14cbPublication20.500.12494/41401oai:repository.ucc.edu.co:20.500.12494/414012024-08-20 16:17:08.885metadata.onlyhttps://repository.ucc.edu.coRepositorio Institucional Universidad Cooperativa de Colombiabdigital@metabiblioteca.com
dc.title.spa.fl_str_mv Molecular and in vitro characterization of field isolates of bovine herpesvirus-1.
title Molecular and in vitro characterization of field isolates of bovine herpesvirus-1.
spellingShingle Molecular and in vitro characterization of field isolates of bovine herpesvirus-1.
Herpesvirus bovino 1
Infección genital Rinfección respiratoria
Infección latente
title_short Molecular and in vitro characterization of field isolates of bovine herpesvirus-1.
title_full Molecular and in vitro characterization of field isolates of bovine herpesvirus-1.
title_fullStr Molecular and in vitro characterization of field isolates of bovine herpesvirus-1.
title_full_unstemmed Molecular and in vitro characterization of field isolates of bovine herpesvirus-1.
title_sort Molecular and in vitro characterization of field isolates of bovine herpesvirus-1.
dc.creator.fl_str_mv Ruiz Saenz, Julian
Jaime, Jairo
Ramirez, Gloria
Vera, Victor
dc.contributor.author.none.fl_str_mv Ruiz Saenz, Julian
Jaime, Jairo
Ramirez, Gloria
Vera, Victor
dc.subject.spa.fl_str_mv Herpesvirus bovino 1
Infección genital Rinfección respiratoria
Infección latente
topic Herpesvirus bovino 1
Infección genital Rinfección respiratoria
Infección latente
description Bovine Herpesvirus-1 (BoHV-1) is distributed worldwide and is a major pathogen in cattle, being the causal agent of a variety of clinical syndromes. The aim of this study was to isolate and to characterize (molecular and biological characterization) BoHV-1 from 29 immunosuppressed animals. It was possible to obtain 18 isolates, each from a different animal, such as from the respiratory and reproductive tracts. In some cases the cytopathic effect was visible 12 hours post-inoculation, and became characteristic after 36-48 hours. Biological characteristics were evaluated and compared with Iowa and Colorado-1 reference strains, and differences were found in plaque size, virus titer measured by TCID50 and PFU/mL, and one step virus curves. These results showed that some isolates had a highly virulent-like behavior in vitro, compared to the reference strains, with shorter eclipse periods, faster release of virus into the supernatants, and higher burst size and viral titer. There were no differences in glycoprotein expression of BoHV-1 isolates, measured by Western blot on monolayers. Moreover, using restriction endonucleases analysis, most of the viruses were confirmed as BoHV-1.1 and just one of them was confirmed as BoHV-1.2a subtype. These findings suggest that some wild-type BoHV-1 isolates could be useful as seeds to develop new monovalent vaccines.
publishDate 2012
dc.date.issued.none.fl_str_mv 2012
dc.date.accessioned.none.fl_str_mv 2021-12-16T22:15:29Z
dc.date.available.none.fl_str_mv 2021-12-16T22:15:29Z
dc.type.none.fl_str_mv Artículo
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dc.type.driver.none.fl_str_mv info:eu-repo/semantics/article
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format http://purl.org/coar/resource_type/c_6501
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dc.identifier.none.fl_str_mv https://doi.org/10.1016/j.acci.2018.05.002
dc.identifier.issn.spa.fl_str_mv 16740769
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/20.500.12494/41401
dc.identifier.bibliographicCitation.spa.fl_str_mv Ruiz J,Jaime J,Ramirez G,Vera V. Molecular and in vitro characterization of field isolates of bovine herpesvirus-1. Virol Sin. 2012. 27. (1):p. 26-37. .
url https://doi.org/10.1016/j.acci.2018.05.002
https://hdl.handle.net/20.500.12494/41401
identifier_str_mv 16740769
Ruiz J,Jaime J,Ramirez G,Vera V. Molecular and in vitro characterization of field isolates of bovine herpesvirus-1. Virol Sin. 2012. 27. (1):p. 26-37. .
dc.relation.ispartofjournal.spa.fl_str_mv Virologica Sinica
dc.rights.accessrights.none.fl_str_mv info:eu-repo/semantics/closedAccess
dc.rights.coar.none.fl_str_mv http://purl.org/coar/access_right/c_14cb
eu_rights_str_mv closedAccess
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dc.format.extent.spa.fl_str_mv 37-26
dc.publisher.spa.fl_str_mv Ko hsueh chu pan she
institution Universidad Cooperativa de Colombia
repository.name.fl_str_mv Repositorio Institucional Universidad Cooperativa de Colombia
repository.mail.fl_str_mv bdigital@metabiblioteca.com
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