Molecular and in vitro characterization of field isolates of bovine herpesvirus-1.
Bovine Herpesvirus-1 (BoHV-1) is distributed worldwide and is a major pathogen in cattle, being the causal agent of a variety of clinical syndromes. The aim of this study was to isolate and to characterize (molecular and biological characterization) BoHV-1 from 29 immunosuppressed animals. It was po...
- Autores:
-
Ruiz Saenz, Julian
Jaime, Jairo
Ramirez, Gloria
Vera, Victor
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2012
- Institución:
- Universidad Cooperativa de Colombia
- Repositorio:
- Repositorio UCC
- Idioma:
- OAI Identifier:
- oai:repository.ucc.edu.co:20.500.12494/41401
- Acceso en línea:
- https://doi.org/10.1016/j.acci.2018.05.002
https://hdl.handle.net/20.500.12494/41401
- Palabra clave:
- Herpesvirus bovino 1
Infección genital Rinfección respiratoria
Infección latente
- Rights
- closedAccess
- License
- http://purl.org/coar/access_right/c_14cb
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Ruiz Saenz, JulianJaime, JairoRamirez, GloriaVera, Victor2021-12-16T22:15:29Z2021-12-16T22:15:29Z2012https://doi.org/10.1016/j.acci.2018.05.00216740769https://hdl.handle.net/20.500.12494/41401Ruiz J,Jaime J,Ramirez G,Vera V. Molecular and in vitro characterization of field isolates of bovine herpesvirus-1. Virol Sin. 2012. 27. (1):p. 26-37. .Bovine Herpesvirus-1 (BoHV-1) is distributed worldwide and is a major pathogen in cattle, being the causal agent of a variety of clinical syndromes. The aim of this study was to isolate and to characterize (molecular and biological characterization) BoHV-1 from 29 immunosuppressed animals. It was possible to obtain 18 isolates, each from a different animal, such as from the respiratory and reproductive tracts. In some cases the cytopathic effect was visible 12 hours post-inoculation, and became characteristic after 36-48 hours. Biological characteristics were evaluated and compared with Iowa and Colorado-1 reference strains, and differences were found in plaque size, virus titer measured by TCID50 and PFU/mL, and one step virus curves. These results showed that some isolates had a highly virulent-like behavior in vitro, compared to the reference strains, with shorter eclipse periods, faster release of virus into the supernatants, and higher burst size and viral titer. There were no differences in glycoprotein expression of BoHV-1 isolates, measured by Western blot on monolayers. Moreover, using restriction endonucleases analysis, most of the viruses were confirmed as BoHV-1.1 and just one of them was confirmed as BoHV-1.2a subtype. These findings suggest that some wild-type BoHV-1 isolates could be useful as seeds to develop new monovalent vaccines.0000-0002-1447-1458julian.ruizs@campusucc.edu.co37-26Ko hsueh chu pan sheHerpesvirus bovino 1Infección genital Rinfección respiratoriaInfección latenteMolecular and in vitro characterization of field isolates of bovine herpesvirus-1.Artículohttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionVirologica Sinicainfo:eu-repo/semantics/closedAccesshttp://purl.org/coar/access_right/c_14cbPublication20.500.12494/41401oai:repository.ucc.edu.co:20.500.12494/414012024-08-20 16:17:08.885metadata.onlyhttps://repository.ucc.edu.coRepositorio Institucional Universidad Cooperativa de Colombiabdigital@metabiblioteca.com |
dc.title.spa.fl_str_mv |
Molecular and in vitro characterization of field isolates of bovine herpesvirus-1. |
title |
Molecular and in vitro characterization of field isolates of bovine herpesvirus-1. |
spellingShingle |
Molecular and in vitro characterization of field isolates of bovine herpesvirus-1. Herpesvirus bovino 1 Infección genital Rinfección respiratoria Infección latente |
title_short |
Molecular and in vitro characterization of field isolates of bovine herpesvirus-1. |
title_full |
Molecular and in vitro characterization of field isolates of bovine herpesvirus-1. |
title_fullStr |
Molecular and in vitro characterization of field isolates of bovine herpesvirus-1. |
title_full_unstemmed |
Molecular and in vitro characterization of field isolates of bovine herpesvirus-1. |
title_sort |
Molecular and in vitro characterization of field isolates of bovine herpesvirus-1. |
dc.creator.fl_str_mv |
Ruiz Saenz, Julian Jaime, Jairo Ramirez, Gloria Vera, Victor |
dc.contributor.author.none.fl_str_mv |
Ruiz Saenz, Julian Jaime, Jairo Ramirez, Gloria Vera, Victor |
dc.subject.spa.fl_str_mv |
Herpesvirus bovino 1 Infección genital Rinfección respiratoria Infección latente |
topic |
Herpesvirus bovino 1 Infección genital Rinfección respiratoria Infección latente |
description |
Bovine Herpesvirus-1 (BoHV-1) is distributed worldwide and is a major pathogen in cattle, being the causal agent of a variety of clinical syndromes. The aim of this study was to isolate and to characterize (molecular and biological characterization) BoHV-1 from 29 immunosuppressed animals. It was possible to obtain 18 isolates, each from a different animal, such as from the respiratory and reproductive tracts. In some cases the cytopathic effect was visible 12 hours post-inoculation, and became characteristic after 36-48 hours. Biological characteristics were evaluated and compared with Iowa and Colorado-1 reference strains, and differences were found in plaque size, virus titer measured by TCID50 and PFU/mL, and one step virus curves. These results showed that some isolates had a highly virulent-like behavior in vitro, compared to the reference strains, with shorter eclipse periods, faster release of virus into the supernatants, and higher burst size and viral titer. There were no differences in glycoprotein expression of BoHV-1 isolates, measured by Western blot on monolayers. Moreover, using restriction endonucleases analysis, most of the viruses were confirmed as BoHV-1.1 and just one of them was confirmed as BoHV-1.2a subtype. These findings suggest that some wild-type BoHV-1 isolates could be useful as seeds to develop new monovalent vaccines. |
publishDate |
2012 |
dc.date.issued.none.fl_str_mv |
2012 |
dc.date.accessioned.none.fl_str_mv |
2021-12-16T22:15:29Z |
dc.date.available.none.fl_str_mv |
2021-12-16T22:15:29Z |
dc.type.none.fl_str_mv |
Artículo |
dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
dc.type.coar.none.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
dc.type.coarversion.none.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.driver.none.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.redcol.none.fl_str_mv |
http://purl.org/redcol/resource_type/ART |
dc.type.version.none.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
http://purl.org/coar/resource_type/c_6501 |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
https://doi.org/10.1016/j.acci.2018.05.002 |
dc.identifier.issn.spa.fl_str_mv |
16740769 |
dc.identifier.uri.none.fl_str_mv |
https://hdl.handle.net/20.500.12494/41401 |
dc.identifier.bibliographicCitation.spa.fl_str_mv |
Ruiz J,Jaime J,Ramirez G,Vera V. Molecular and in vitro characterization of field isolates of bovine herpesvirus-1. Virol Sin. 2012. 27. (1):p. 26-37. . |
url |
https://doi.org/10.1016/j.acci.2018.05.002 https://hdl.handle.net/20.500.12494/41401 |
identifier_str_mv |
16740769 Ruiz J,Jaime J,Ramirez G,Vera V. Molecular and in vitro characterization of field isolates of bovine herpesvirus-1. Virol Sin. 2012. 27. (1):p. 26-37. . |
dc.relation.ispartofjournal.spa.fl_str_mv |
Virologica Sinica |
dc.rights.accessrights.none.fl_str_mv |
info:eu-repo/semantics/closedAccess |
dc.rights.coar.none.fl_str_mv |
http://purl.org/coar/access_right/c_14cb |
eu_rights_str_mv |
closedAccess |
rights_invalid_str_mv |
http://purl.org/coar/access_right/c_14cb |
dc.format.extent.spa.fl_str_mv |
37-26 |
dc.publisher.spa.fl_str_mv |
Ko hsueh chu pan she |
institution |
Universidad Cooperativa de Colombia |
repository.name.fl_str_mv |
Repositorio Institucional Universidad Cooperativa de Colombia |
repository.mail.fl_str_mv |
bdigital@metabiblioteca.com |
_version_ |
1814246720787185664 |