Use of the mitochondrial serine transfer RNA (UCN) of Lutzomyia columbiana (Diptera, Psychodidae) in phylogeographic studies

Ponencia Internacional

Autores:
Rosero Galindo, Carol Yovanna
García López, Juan Pablo
Montenegro Coral, Franco Andrés
Tipo de recurso:
http://purl.org/coar/resource_type/c_c94f
Fecha de publicación:
2014
Institución:
Universidad Cooperativa de Colombia
Repositorio:
Repositorio UCC
Idioma:
OAI Identifier:
oai:repository.ucc.edu.co:20.500.12494/1578
Acceso en línea:
https://hdl.handle.net/20.500.12494/1578
Palabra clave:
vectors of Leishmania sp
diseases in human
genetic diversity
mitochondrial gene
Rights
openAccess
License
Licencia CC
id COOPER2_e1cb9d867c4ba0ee46013e8622393fe7
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network_acronym_str COOPER2
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repository_id_str
spelling Rosero Galindo, Carol YovannaGarcía López, Juan PabloMontenegro Coral, Franco Andrés2017-10-13T12:55:45Z2017-10-13T12:55:45Z2014-08https://hdl.handle.net/20.500.12494/1578Ponencia InternacionalBACKGROUND: Sand flies are small diptera of Psychodidae family of medical importance due to their role like vectors of Leishmania sp., Acording to the low knowledge of molecular characteristics of this specie in our region, the principal main of this study was evaluate the genetic diversity of mitochondrial gene encoding serine (ser-tRNA), in natural populations of Lu. columbiana in Nariño, southern Colombia. METHODS: The species status was confirmed by using the taxonomic key generated by Young and Duncan (1994) and Galati (2003). The genetic analysis was performed by amplification by PCRof gene ser-tRNA using the primers identified by Ready et al (1997), which have as target sequence the 3' end by the 3' Cyt b'subunit 1 gene of NADH dehydrogenase (NAD). The same conditions for PCR reported by Perez (2008) were used. RESULTS: The ser-tRNA´s gene amplification was necessary to modify the PCR conditions reported by Pérez (2008). In a final volume of 12.5 ul used 1X PCR buffer, 1.5 mM MgCl2, 1.5 U of Taq polymerase ( Promega GoTaq Kit), 0.2 mm dNTP's (Promega ), 0.3 um of each primer and 6ul of DNA. The ser-tRNA´s gene consisted of 67 base pairs. The sequencing analysis show value of S:0 to number of polymorphic sites, Eta:0 to total number of mutations, h:1 to number of haplotypes, and Haplotype (gene) diversity, both variance of haplotype diversity and standard deviation of haplotype diversity values below 0. CONCLUSIONS: The number of haplotypes found in populations of Lu. columbiana exhibited genetic variance possibly mainly due to geographical diferences, however is necessary increase the number of individuals analyzed to confirm the utility of the gene in phylogeographic studies.carol.roserog@campusucc.edu.coGrupo Interdisciplinario de Investigación en Salud-Enfermedad, Facultad de Medicina, Universidad Cooperativa de Colombia, 520002 Pasto, Colombia.MedicinaPastovectors of Leishmania spdiseases in humangenetic diversitymitochondrial geneUse of the mitochondrial serine transfer RNA (UCN) of Lutzomyia columbiana (Diptera, Psychodidae) in phylogeographic studiesPonenciahttp://purl.org/coar/resource_type/c_c94finfo:eu-repo/semantics/conferenceObjectinfo:eu-repo/semantics/acceptedVersionLicencia CCinfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2PublicationLICENSElicense.txtlicense.txttext/plain; charset=utf-84553https://repository.ucc.edu.co/bitstreams/c6e9e111-85e3-43f0-a023-cd4903b97ce9/downloadcb8f4d9b72d863ad6737f22af442cc82MD52TEXTUse of the mitochondrial, 2014.pdf.txtUse of the mitochondrial, 2014.pdf.txtExtracted texttext/plain706https://repository.ucc.edu.co/bitstreams/57b6080c-e8eb-4cf9-8bbf-0d5fc2db3833/download29bfb7507957274c05374d6173c52c16MD53ORIGINALUse of the mitochondrial, 2014.pdfUse of the mitochondrial, 2014.pdfapplication/pdf220692https://repository.ucc.edu.co/bitstreams/304584e1-1d78-466d-ab08-6a45ddacb920/download771a3358c030b3615a6f6f4cedfeb289MD51THUMBNAILUse of the mitochondrial, 2014.pdf.jpgUse of the mitochondrial, 2014.pdf.jpgIM Thumbnailimage/jpeg17799https://repository.ucc.edu.co/bitstreams/bd4edff9-edc0-4188-9f71-c204197f5387/download9f56b7a4860cf12de777b765bed3a0d4MD5420.500.12494/1578oai:repository.ucc.edu.co:20.500.12494/15782024-08-10 22:52:07.799restrictedhttps://repository.ucc.edu.coRepositorio Institucional Universidad Cooperativa de Colombiabdigital@metabiblioteca.com
dc.title.eng.fl_str_mv Use of the mitochondrial serine transfer RNA (UCN) of Lutzomyia columbiana (Diptera, Psychodidae) in phylogeographic studies
title Use of the mitochondrial serine transfer RNA (UCN) of Lutzomyia columbiana (Diptera, Psychodidae) in phylogeographic studies
spellingShingle Use of the mitochondrial serine transfer RNA (UCN) of Lutzomyia columbiana (Diptera, Psychodidae) in phylogeographic studies
vectors of Leishmania sp
diseases in human
genetic diversity
mitochondrial gene
title_short Use of the mitochondrial serine transfer RNA (UCN) of Lutzomyia columbiana (Diptera, Psychodidae) in phylogeographic studies
title_full Use of the mitochondrial serine transfer RNA (UCN) of Lutzomyia columbiana (Diptera, Psychodidae) in phylogeographic studies
title_fullStr Use of the mitochondrial serine transfer RNA (UCN) of Lutzomyia columbiana (Diptera, Psychodidae) in phylogeographic studies
title_full_unstemmed Use of the mitochondrial serine transfer RNA (UCN) of Lutzomyia columbiana (Diptera, Psychodidae) in phylogeographic studies
title_sort Use of the mitochondrial serine transfer RNA (UCN) of Lutzomyia columbiana (Diptera, Psychodidae) in phylogeographic studies
dc.creator.fl_str_mv Rosero Galindo, Carol Yovanna
García López, Juan Pablo
Montenegro Coral, Franco Andrés
dc.contributor.author.none.fl_str_mv Rosero Galindo, Carol Yovanna
García López, Juan Pablo
Montenegro Coral, Franco Andrés
dc.subject.spa.fl_str_mv vectors of Leishmania sp
diseases in human
genetic diversity
mitochondrial gene
topic vectors of Leishmania sp
diseases in human
genetic diversity
mitochondrial gene
description Ponencia Internacional
publishDate 2014
dc.date.issued.none.fl_str_mv 2014-08
dc.date.accessioned.none.fl_str_mv 2017-10-13T12:55:45Z
dc.date.available.none.fl_str_mv 2017-10-13T12:55:45Z
dc.type.none.fl_str_mv Ponencia
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url https://hdl.handle.net/20.500.12494/1578
dc.rights.cc.none.fl_str_mv Licencia CC
dc.rights.accessrights.none.fl_str_mv info:eu-repo/semantics/openAccess
dc.rights.coar.none.fl_str_mv http://purl.org/coar/access_right/c_abf2
rights_invalid_str_mv Licencia CC
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.publisher.spa.fl_str_mv Grupo Interdisciplinario de Investigación en Salud-Enfermedad, Facultad de Medicina, Universidad Cooperativa de Colombia, 520002 Pasto, Colombia.
dc.publisher.program.spa.fl_str_mv Medicina
dc.publisher.place.spa.fl_str_mv Pasto
institution Universidad Cooperativa de Colombia
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