Immune characterization of a Colombian family cluster with SARS-CoV-2 infection

Introduction: Immunological markers have been described during COVID-19 and persist after recovery. These immune markers are associated with clinical features among SARSCoV- 2 infected individuals. Nevertheless, studies reporting a comprehensive analysis of the immune changes occurring during SARS-C...

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Autores:
Aguilar Jiménez, Wbeimar
Flórez Álvarez, Lizdany
Rincón, Daniel S.
Marín Palma, Damariz
Sanchez Martinez, Alexandra
Martínez Moreno, Jahnnyer
Zapata Cardona, María Isabel
Loaiza Durán, John Darío
Cardenas, Constanza
Guzman, Fanny
Velilla Hernandez, Paula Andrea
Taborda, Natalia Andrea
Zapata Builes, Wildeman
Hernández López, Juan Carlos
Diaz, Francisco Javier
Rugeles López, María Teresa
Tipo de recurso:
Article of investigation
Fecha de publicación:
2021
Institución:
Universidad Cooperativa de Colombia
Repositorio:
Repositorio UCC
Idioma:
OAI Identifier:
oai:repository.ucc.edu.co:20.500.12494/43606
Acceso en línea:
https://hdl.handle.net/20.500.12494/43606
Palabra clave:
Coronavirus infections
Inflammation
Killer cells, natural
T-lymphocytes
Antibodies
Neutralizing
Coronavirus infections
Inflammation
Killer cells, natural
T-lymphocytes
Antibodies
Neutralizing
Rights
openAccess
License
Atribución
id COOPER2_dbfdffffb574bf053f469705b926bcc5
oai_identifier_str oai:repository.ucc.edu.co:20.500.12494/43606
network_acronym_str COOPER2
network_name_str Repositorio UCC
repository_id_str
dc.title.spa.fl_str_mv Immune characterization of a Colombian family cluster with SARS-CoV-2 infection
title Immune characterization of a Colombian family cluster with SARS-CoV-2 infection
spellingShingle Immune characterization of a Colombian family cluster with SARS-CoV-2 infection
Coronavirus infections
Inflammation
Killer cells, natural
T-lymphocytes
Antibodies
Neutralizing
Coronavirus infections
Inflammation
Killer cells, natural
T-lymphocytes
Antibodies
Neutralizing
title_short Immune characterization of a Colombian family cluster with SARS-CoV-2 infection
title_full Immune characterization of a Colombian family cluster with SARS-CoV-2 infection
title_fullStr Immune characterization of a Colombian family cluster with SARS-CoV-2 infection
title_full_unstemmed Immune characterization of a Colombian family cluster with SARS-CoV-2 infection
title_sort Immune characterization of a Colombian family cluster with SARS-CoV-2 infection
dc.creator.fl_str_mv Aguilar Jiménez, Wbeimar
Flórez Álvarez, Lizdany
Rincón, Daniel S.
Marín Palma, Damariz
Sanchez Martinez, Alexandra
Martínez Moreno, Jahnnyer
Zapata Cardona, María Isabel
Loaiza Durán, John Darío
Cardenas, Constanza
Guzman, Fanny
Velilla Hernandez, Paula Andrea
Taborda, Natalia Andrea
Zapata Builes, Wildeman
Hernández López, Juan Carlos
Diaz, Francisco Javier
Rugeles López, María Teresa
dc.contributor.author.none.fl_str_mv Aguilar Jiménez, Wbeimar
Flórez Álvarez, Lizdany
Rincón, Daniel S.
Marín Palma, Damariz
Sanchez Martinez, Alexandra
Martínez Moreno, Jahnnyer
Zapata Cardona, María Isabel
Loaiza Durán, John Darío
Cardenas, Constanza
Guzman, Fanny
Velilla Hernandez, Paula Andrea
Taborda, Natalia Andrea
Zapata Builes, Wildeman
Hernández López, Juan Carlos
Diaz, Francisco Javier
Rugeles López, María Teresa
dc.subject.spa.fl_str_mv Coronavirus infections
Inflammation
Killer cells, natural
T-lymphocytes
Antibodies
Neutralizing
topic Coronavirus infections
Inflammation
Killer cells, natural
T-lymphocytes
Antibodies
Neutralizing
Coronavirus infections
Inflammation
Killer cells, natural
T-lymphocytes
Antibodies
Neutralizing
dc.subject.other.spa.fl_str_mv Coronavirus infections
Inflammation
Killer cells, natural
T-lymphocytes
Antibodies
Neutralizing
description Introduction: Immunological markers have been described during COVID-19 and persist after recovery. These immune markers are associated with clinical features among SARSCoV- 2 infected individuals. Nevertheless, studies reporting a comprehensive analysis of the immune changes occurring during SARS-CoV-2 infection are still limited. Objective: To evaluate the production of proinflammatory cytokines, the antibody response, and the phenotype and function of NK cells and T cells in a Colombian family cluster with SARS-CoV-2 infection. Materials and methods: Proinflammatory cytokines were evaluated by RT-PCR and ELISA. The frequency, phenotype, and function of NK cells (cocultures with K562 cells) and T-cells (stimulated with spike/RdRp peptides) were assessed by flow cytometry. Anti-SARS-CoV-2 antibodies were determined using indirect immunofluorescence and plaque reduction neutralization assay. Results: During COVID-19, we observed a high proinflammatory-cytokine production and a reduced CD56bright-NK cell and cytotoxic response. Compared with healthy controls, infected individuals had a higher frequency of dysfunctional CD8+ T cells CD38+HLA-DR-. During the acute phase, CD8+ T cells stimulated with viral peptides exhibited a monofunctional response characterized by high IL-10 production. However, during recovery, we observed a bifunctional response characterized by the co-expression of CD107a and granzyme B or perforin. Conclusion: Although the proinflammatory response is a hallmark of SARS-CoV-2 infection, other phenotypic and functional alterations in NK cells and CD8+ T cells could be associated with the outcome of COVID-19. However, additional studies are required to understand these alterations and to guide future immunotherapy strategies.
publishDate 2021
dc.date.issued.none.fl_str_mv 2021-06-09
dc.date.accessioned.none.fl_str_mv 2022-02-02T21:15:12Z
dc.date.available.none.fl_str_mv 2022-02-02T21:15:12Z
dc.type.none.fl_str_mv Artículos Científicos
dc.type.coar.none.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
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dc.identifier.issn.spa.fl_str_mv 0120-4157
dc.identifier.uri.spa.fl_str_mv 10.7705/biomedica.5976
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/20.500.12494/43606
dc.identifier.bibliographicCitation.spa.fl_str_mv Aguilar Jiménez, W., Florez Álvarez, L., Rincón, D.S., Marín Palma, D., Sánchez Martínez, A., Martínez, J. et al. Immune characterization of a Colombian family cluster with SARS-CoV-2 infection. Biom dica. 2021;41:86-102. https://doi.org/10.7705/biomedica.5976
identifier_str_mv 0120-4157
10.7705/biomedica.5976
Aguilar Jiménez, W., Florez Álvarez, L., Rincón, D.S., Marín Palma, D., Sánchez Martínez, A., Martínez, J. et al. Immune characterization of a Colombian family cluster with SARS-CoV-2 infection. Biom dica. 2021;41:86-102. https://doi.org/10.7705/biomedica.5976
url https://hdl.handle.net/20.500.12494/43606
dc.relation.isversionof.spa.fl_str_mv https://revistabiomedica.org/index.php/biomedica/article/view/5976
dc.relation.ispartofjournal.spa.fl_str_mv BIOMEDICA
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4. Chu H, Chan JFW, Wang Y, Yuen TT, Chai Y, Hou Y, et al. Comparative replication and immune activation profiles of SARS-CoV-2 and SARS-CoV in human lungs: An ex vivo study with implications for the pathogenesis of COVID-19. Clin Infect Dis. 2020;71:1400-9. https://doi.org/10.1093/cid/ciaa410
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20. D az FJ, Aguilar-Jim nez W, Fl rez- lvarez L, Valencia G, Laiton-Donato K, Franco-Mu oz C, et al. Aislamiento y caracterizaci n de una cepa temprana de SARS-CoV-2 durante la epidemia de 2020 en Medell n, Colombia. Biom dica. 2020;40(Supl.2):148-58. https://doi.org/10.7705/biomedica.5834
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27. Ge H, Wang X, Yuan X, Xiao G, Wang C, Deng T, et al. The epidemiology and clinical information about COVID-19. Eur J Clin Microbiol Infect Dis. 2020;39:1011-9. https://doi.org/10.1007/s10096-020-03874-z
28. Garc a LF. Immune response, inflammation, and the clinical spectrum of COVID-19. Front Immunol. 2020;11:4-8. https://doi.org/10.3389/fimmu.2020.01441
29. Azkur AK, Akdis M, Azkur D, Sokolowska M, van de Veen W, Brüggen MC, et al. Immune response to SARS-CoV-2 and mechanisms of immunopathological changes in COVID-19. Allergy Eur J Allergy Clin Immunol. 2020;75:1564-81. https://doi.org/10.1111/all.14364
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41. De Biasi S, Meschiari M, Gibellini L, Bellinazzi C, Borella R, Fidanza L, et al. Marked T cell activation, senescence, exhaustion and skewing towards TH17 in patients with COVID-19 pneumonia. Nat Commun. 2020;11:1-17. https://doi.org/10.1038/s41467-020-17292-4
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Instituto Nacional de Salud
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dc.publisher.place.spa.fl_str_mv Medellín
institution Universidad Cooperativa de Colombia
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spelling Aguilar Jiménez, WbeimarFlórez Álvarez, LizdanyRincón, Daniel S.Marín Palma, DamarizSanchez Martinez, AlexandraMartínez Moreno, JahnnyerZapata Cardona, María IsabelLoaiza Durán, John DaríoCardenas, ConstanzaGuzman, FannyVelilla Hernandez, Paula AndreaTaborda, Natalia AndreaZapata Builes, WildemanHernández López, Juan CarlosDiaz, Francisco Javier Rugeles López, María Teresa41(Supl. 2)2022-02-02T21:15:12Z2022-02-02T21:15:12Z2021-06-090120-415710.7705/biomedica.5976https://hdl.handle.net/20.500.12494/43606Aguilar Jiménez, W., Florez Álvarez, L., Rincón, D.S., Marín Palma, D., Sánchez Martínez, A., Martínez, J. et al. Immune characterization of a Colombian family cluster with SARS-CoV-2 infection. Biom dica. 2021;41:86-102. https://doi.org/10.7705/biomedica.5976Introduction: Immunological markers have been described during COVID-19 and persist after recovery. These immune markers are associated with clinical features among SARSCoV- 2 infected individuals. Nevertheless, studies reporting a comprehensive analysis of the immune changes occurring during SARS-CoV-2 infection are still limited. Objective: To evaluate the production of proinflammatory cytokines, the antibody response, and the phenotype and function of NK cells and T cells in a Colombian family cluster with SARS-CoV-2 infection. Materials and methods: Proinflammatory cytokines were evaluated by RT-PCR and ELISA. The frequency, phenotype, and function of NK cells (cocultures with K562 cells) and T-cells (stimulated with spike/RdRp peptides) were assessed by flow cytometry. Anti-SARS-CoV-2 antibodies were determined using indirect immunofluorescence and plaque reduction neutralization assay. Results: During COVID-19, we observed a high proinflammatory-cytokine production and a reduced CD56bright-NK cell and cytotoxic response. Compared with healthy controls, infected individuals had a higher frequency of dysfunctional CD8+ T cells CD38+HLA-DR-. During the acute phase, CD8+ T cells stimulated with viral peptides exhibited a monofunctional response characterized by high IL-10 production. However, during recovery, we observed a bifunctional response characterized by the co-expression of CD107a and granzyme B or perforin. Conclusion: Although the proinflammatory response is a hallmark of SARS-CoV-2 infection, other phenotypic and functional alterations in NK cells and CD8+ T cells could be associated with the outcome of COVID-19. However, additional studies are required to understand these alterations and to guide future immunotherapy strategies.Introduction: Immunological markers have been described during COVID-19 and persist after recovery. These immune markers are associated with clinical features among SARSCoV- 2 infected individuals. Nevertheless, studies reporting a comprehensive analysis of the immune changes occurring during SARS-CoV-2 infection are still limited. Objective: To evaluate the production of proinflammatory cytokines, the antibody response, and the phenotype and function of NK cells and T cells in a Colombian family cluster with SARS-CoV-2 infection. Materials and methods: Proinflammatory cytokines were evaluated by RT-PCR and ELISA. The frequency, phenotype, and function of NK cells (cocultures with K562 cells) and T-cells (stimulated with spike/RdRp peptides) were assessed by flow cytometry. Anti-SARS-CoV-2 antibodies were determined using indirect immunofluorescence and plaque reduction neutralization assay. Results: During COVID-19, we observed a high proinflammatory-cytokine production and a reduced CD56bright-NK cell and cytotoxic response. Compared with healthy controls, infected individuals had a higher frequency of dysfunctional CD8+ T cells CD38+HLA-DR-. During the acute phase, CD8+ T cells stimulated with viral peptides exhibited a monofunctional response characterized by high IL-10 production. However, during recovery, we observed a bifunctional response characterized by the co-expression of CD107a and granzyme B or perforin. Conclusion: Although the proinflammatory response is a hallmark of SARS-CoV-2 infection, other phenotypic and functional alterations in NK cells and CD8+ T cells could be associated with the outcome of COVID-19. However, additional studies are required to understand these alterations and to guide future immunotherapy strategies.https://scienti.minciencias.gov.co/cvlac/EnProdArticulo/query.do?cod_producto=73&cod_rh=0000157775https://orcid.org/0000-0002-7351-8738COL0112548wildeman.zapatab@campusucc.edu.cohttps://scholar.google.com.co/citations?hl=en&user=VLZxl1UAAAAJ17Grupo Infettare, Facultad de Medicina, Universidad Cooperativa de Colombia, Medellin, ColombiaInstituto Nacional de SaludMedicinaMedellínhttps://revistabiomedica.org/index.php/biomedica/article/view/5976BIOMEDICA1. Li Q, Guan X, Wu P, Wang X, Zhou L, Tong Y, et al. Early transmission dynamics in Wuhan, China, of novel coronavirus-infected pneumonia. N Engl J Med. 2020;382:1199-207. https://doi.org/10.1056/NEJMoa20013162. Chen Y, Liu Q, Guo D. Emerging coronaviruses: Genome structure, replication, and pathogenesis. J Med Virol. 2020;92:418-23. https://doi.org/10.1002/jmv.256813. Ortiz ME, Thurman A, Pezzulo AA, Leidinger MR, Klesney-Tait JA, Karp PH, et al. Heterogeneous expression of the SARS-Coronavirus-2 receptor ACE2 in the human respiratory tract. EBioMedicine. 2020;60:102976. https://doi.org/10.1016/j.ebiom.2020.1029764. Chu H, Chan JFW, Wang Y, Yuen TT, Chai Y, Hou Y, et al. Comparative replication and immune activation profiles of SARS-CoV-2 and SARS-CoV in human lungs: An ex vivo study with implications for the pathogenesis of COVID-19. Clin Infect Dis. 2020;71:1400-9. https://doi.org/10.1093/cid/ciaa4105. Huang C, Wang Y, Li X, Ren L, Zhao J, Hu Y, et al. Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China. Lancet. 2020;395:497-506. https://doi.org/10.1016/S0140-6736(20)30183-56. Yuan X, Tong X, Wang Y, Wang H, Wang L, Xu X. Coagulopathy in elderly patients with coronavirus disease 2019. AGING Med. 2020;3:260-5. https://doi.org/10.1002/agm2.121337. Ponti G, Maccaferri M, Ruini C, Tomasi A, Ozben T. Biomarkers associated with COVID-19 disease progression. Crit Rev Clin Lab Sci. 2020;57:1-11. https://doi.org/10.1080/10408363.2020.17706858. Taborda NA, Hern ndez JC, Montoya CJ, Rugeles MT. Natural killer cells and their role in the immune response during human immunodeficiency virus type-1 infection. Inmunologia. 2014;33:11-20. https://doi.org/10.1016/j.inmuno.2013.11.0029. Alrubayyi A. NK cells in COVID-19: Protectors or opponents? Nat Rev Immunol. 2020;20:520. https://doi.org/10.1038/s41577-020-0408-010. Della Chiesa M, Sivori S, Carlomagno S, Moretta L, Moretta A. 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