HIV-1-derived single-stranded RNA acts as activator of human neutrophils

Neutrophils are key effector cells of the innate immune system and are involved in the host defense against invading pathogens such as viruses. Recently, it was reported that HIV-1–neutrophil interaction triggers neutrophil activation and promotes expression of Toll-like receptors (TLRs). Here, we assessed the role of single-stranded RNA40 (ssRNA40) derived from HIV-1 in neutrophil activation. We observed functional activation of neutrophils in response to HIV-1-derived ssRNA40 based on the expression of TLR7/8, RIG-I, and MDA5, induction of cytokines (IL-6 and TNF-a), and the production of reactive oxygen species (ROS). Additionally, ssRNA40 promoted the expression of CD62L and TNF-a and the production of ROS in the presence of the TLR2 agonist Pam2CSK4. ssRNA40 together with R848 (a TLR7/8 agonist) increased CD11b expression but decreased CD62L expression. Furthermore, decreased IL-6 expression was observed in the presence of the TLR4 agonist LPS. Finally, we found that ssRNA40 promotes RIG-I and MDA5 expression in the presence of the TLR2, TLR4 and TLR7/8 agonists. This study demonstrates a functional response of TLRs in neutrophils challenged with ssRNA40, suggesting that TLRs could be involved in the innate immune response observed during HIV infection, which might be mediated by its genome. © 2016, Springer Science+Business Media New York.

• Autores: Giraldo D.M.; Hernández López, Juan Carlos; Urcuqui-Inchima S.
• Tipo de recurso: Article of journal
• Fecha de publicación: 2016
• Institución: Universidad Cooperativa de Colombia
• Repositorio: Repositorio UCC
• OAI Identifier: oai:repository.ucc.edu.co:20.500.12494/41399
• Acceso en línea: https://doi.org/10.1016/j.acci.2016.06.001; http://www.scielo.org.co/pdf/recis/v17n2/1692-7273-recis-17-02-245.pdf; https://hdl.handle.net/20.500.12494/41399
• Palabra clave: CD11b antigen; interleukin 6; L selectin; marker; MDA5 protein; messenger RNA; reactive oxygen metabolite; retinoic acid inducible protein I; single stranded RNA; toll like receptor 2; toll like receptor 4; toll like receptor 7; toll like receptor 8; tumor necrosis factor; unclassified drug; adult; Article; cytokine release; female; human; human cell; Human immunodeficiency virus 1; leukocyte activation; male; normal human; priority journal
• Rights: closedAccess
• License: http://purl.org/coar/access_right/c_14cb