Usefulness of an in vitro-transcribed RNA control for the detection and quantification of Yellow fever virus through real-time reverse transcription-polymerase chain reaction
Introduction: Unvaccinated individuals in endemic areas with proven enzootic transmission of Yellow fever virus are at risk of infection due to a dramatic shift in the epidemiology of the disease over recent years. For this reason, epidemiological surveillance and laboratory confirmation of cases ha...
- Autores:
-
Laiton Donato, Katherine
Quintero Cortés, Paula
Franco Salazar, Juan Pablo
Peláez Carvajal, Dioselina
Navas, Maria Cristina
Junglen, Sandra
Parra Henao, Gabriel
Usme Ciro, José Aldemar
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2023
- Institución:
- Universidad Cooperativa de Colombia
- Repositorio:
- Repositorio UCC
- Idioma:
- eng
- OAI Identifier:
- oai:repository.ucc.edu.co:20.500.12494/55530
- Acceso en línea:
- https://hdl.handle.net/20.500.12494/55530
- Palabra clave:
- Yellow fever virus
RT-qPCR
Molecular detection
in vitro transcription
Plasmid DNA
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by-nc-nd/4.0/
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Usefulness of an in vitro-transcribed RNA control for the detection and quantification of Yellow fever virus through real-time reverse transcription-polymerase chain reaction |
| title |
Usefulness of an in vitro-transcribed RNA control for the detection and quantification of Yellow fever virus through real-time reverse transcription-polymerase chain reaction |
| spellingShingle |
Usefulness of an in vitro-transcribed RNA control for the detection and quantification of Yellow fever virus through real-time reverse transcription-polymerase chain reaction Yellow fever virus RT-qPCR Molecular detection in vitro transcription Plasmid DNA |
| title_short |
Usefulness of an in vitro-transcribed RNA control for the detection and quantification of Yellow fever virus through real-time reverse transcription-polymerase chain reaction |
| title_full |
Usefulness of an in vitro-transcribed RNA control for the detection and quantification of Yellow fever virus through real-time reverse transcription-polymerase chain reaction |
| title_fullStr |
Usefulness of an in vitro-transcribed RNA control for the detection and quantification of Yellow fever virus through real-time reverse transcription-polymerase chain reaction |
| title_full_unstemmed |
Usefulness of an in vitro-transcribed RNA control for the detection and quantification of Yellow fever virus through real-time reverse transcription-polymerase chain reaction |
| title_sort |
Usefulness of an in vitro-transcribed RNA control for the detection and quantification of Yellow fever virus through real-time reverse transcription-polymerase chain reaction |
| dc.creator.fl_str_mv |
Laiton Donato, Katherine Quintero Cortés, Paula Franco Salazar, Juan Pablo Peláez Carvajal, Dioselina Navas, Maria Cristina Junglen, Sandra Parra Henao, Gabriel Usme Ciro, José Aldemar |
| dc.contributor.author.none.fl_str_mv |
Laiton Donato, Katherine Quintero Cortés, Paula Franco Salazar, Juan Pablo Peláez Carvajal, Dioselina Navas, Maria Cristina Junglen, Sandra Parra Henao, Gabriel Usme Ciro, José Aldemar |
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CIST-Centro de Investigación en Salud para el Trópico |
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Yellow fever virus RT-qPCR Molecular detection in vitro transcription Plasmid DNA |
| topic |
Yellow fever virus RT-qPCR Molecular detection in vitro transcription Plasmid DNA |
| description |
Introduction: Unvaccinated individuals in endemic areas with proven enzootic transmission of Yellow fever virus are at risk of infection due to a dramatic shift in the epidemiology of the disease over recent years. For this reason, epidemiological surveillance and laboratory confirmation of cases have become mandatory. Objective: To develop and test a control RNA for YFV detection through real-time RT-PCR. Methods: A 437-bp insert containing the T7 promoter and the target sequences for two different in-house protocols was designed in the context of the pUC57 vector and obtained through gene synthesis. After T7-driven in vitro transcription, standard curves were developed for Log10 serial dilutions of the YFV control RNA with 8 replicates. Results: A dynamic range of quantification of 10 orders of magnitude was observed with a limit of detection of 6.3 GCE/µL (95% CI, 2.6 to 139.4 GCE/µL). Conclusion: The plasmid construct is available for YFV molecular test validation on clinical, entomological, and epizootic samples. |
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2023 |
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2023-01-26 |
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2024-05-07T01:25:07Z |
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2024-05-07T01:25:07Z |
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Artículo |
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Laiton Donato, K., Quintero Cortés, P., Franco Salazar, J.P., Peláez Carvajal, D., Navas, M.C., Junglen, S., Parra Henao, G. & Usme Ciro, J.A. 2023. Usefulness of an in vitro-transcribed RNA control for the detection and quantification of Yellow fever virus through real-time reverse transcription-polymerase chain reaction. Infectious Diseases Now. 53(3):104654. doi: 10.1016/j.idnow.2023.104654.https://hdl.handle.net/20.500.12494/55530 |
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2666-9927 |
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https://hdl.handle.net/20.500.12494/55530 |
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2666-9919 |
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Laiton Donato, K., Quintero Cortés, P., Franco Salazar, J.P., Peláez Carvajal, D., Navas, M.C., Junglen, S., Parra Henao, G. & Usme Ciro, J.A. 2023. Usefulness of an in vitro-transcribed RNA control for the detection and quantification of Yellow fever virus through real-time reverse transcription-polymerase chain reaction. Infectious Diseases Now. 53(3):104654. doi: 10.1016/j.idnow.2023.104654.https://hdl.handle.net/20.500.12494/55530 2666-9927 doi.org/10.1016/j.idnow.2023.104654 2666-9919 |
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eng |
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eng |
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104654 |
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1 |
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53 |
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Infectious Diseases Now |
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Mutebi J-P, Wang H, Li L, Bryant JE, Barrett ADT. Phylogenetic and Evolutionary Relationships among Yellow Fever Virus Isolates in Africa. J Virol 2001;75:6999–7008. https://doi.org/10.1128/JVI.75.15.6999-7008.2001. Bryant JE, Holmes EC, Barrett ADT. Out of Africa: A Molecular Perspective on the Introduction of Yellow Fever Virus into the Americas. PLoS Pathog 2007;3: e75. https://doi.org/10.1371/journal.ppat.0030075. Paules CI, Fauci AS. Yellow Fever — Once Again on the Radar Screen in the Americas. N Engl J Med 2017;376:1397–9. https://doi.org/10.1056/NEJMp1702172. Faria NR, Kraemer MUG, Hill SC, Goes de Jesus J, Aguiar RS, Iani FCM, et al. Genomic and epidemiological monitoring of yellow fever virus transmission potential. Science (80-) 2018;361:894–9. https://doi.org/10.1126/science.aat7115. Sacchetto L, Silva NIO, de Rezende IM, Arruda MS, Costa TA, de Mello ÉM, et al. Neighbor danger: Yellow fever virus epizootics in urban and urban-rural transition areas of Minas Gerais state, during 2017–2018 yellow fever outbreaks in Brazil. PLoS Negl Trop Dis 2020;14:e0008658. https://doi.org/10.1371/journal.pntd.0008658. Domingo C, Charrel RN, Schmidt-Chanasit J, Zeller H, Reusken C. Yellow fever in the diagnostics laboratory. Emerg Microbes Infect 2018;7:1–15. https://doi.org/10.1038/s41426-018-0128-8. Pan American Health Organization P. Real-time RT-PCR (TaqManTM) protocol -Yellow fever virus (YFV) 2018:1. https://www.paho.org/en/documents/realtime-rt-pcr-taqmantm-protocol-yellow-fever-virus-yfv (accessed May 17, 2022). Organización Panamericana de la Salud OPS. Recomendaciones para la detección y el diagnóstico por laboratorio de infecciones por arbovirus en la Región de las Américas. Washington DC: Pan American Health Organization; 2022. https://doi.org/10.37774/9789275325872. Domingo C, Patel P, Yillah J, Weidmann M, Méndez JA, Nakouné ER, et al. Advanced Yellow Fever Virus Genome Detection in Point-of-Care Facilities and Reference Laboratories. J Clin Microbiol 2012;50:4054–60. https://doi.org/10.1128/JCM.01799-12. Álvarez-Díaz DA, Quintero PA, Peláez-Carvajal D, Ajami NJ, Usme-Ciro JA. Novel pan-serotype control RNA for dengue virus typing through real-time reverse transcription-polymerase chain reaction. J Virol Methods 2019;271:113677. https://doi.org/10.1016/j.jviromet.2019.113677. |
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Laiton Donato, KatherineQuintero Cortés, PaulaFranco Salazar, Juan PabloPeláez Carvajal, DioselinaNavas, Maria CristinaJunglen, SandraParra Henao, GabrielUsme Ciro, José AldemarCIST-Centro de Investigación en Salud para el Trópico2024-05-07T01:25:07Z2024-05-07T01:25:07Z2023-01-26Laiton Donato, K., Quintero Cortés, P., Franco Salazar, J.P., Peláez Carvajal, D., Navas, M.C., Junglen, S., Parra Henao, G. & Usme Ciro, J.A. 2023. Usefulness of an in vitro-transcribed RNA control for the detection and quantification of Yellow fever virus through real-time reverse transcription-polymerase chain reaction. Infectious Diseases Now. 53(3):104654. doi: 10.1016/j.idnow.2023.104654.https://hdl.handle.net/20.500.12494/55530 2666-9927https://hdl.handle.net/20.500.12494/55530doi.org/10.1016/j.idnow.2023.1046542666-9919Introduction: Unvaccinated individuals in endemic areas with proven enzootic transmission of Yellow fever virus are at risk of infection due to a dramatic shift in the epidemiology of the disease over recent years. For this reason, epidemiological surveillance and laboratory confirmation of cases have become mandatory. Objective: To develop and test a control RNA for YFV detection through real-time RT-PCR. Methods: A 437-bp insert containing the T7 promoter and the target sequences for two different in-house protocols was designed in the context of the pUC57 vector and obtained through gene synthesis. After T7-driven in vitro transcription, standard curves were developed for Log10 serial dilutions of the YFV control RNA with 8 replicates. Results: A dynamic range of quantification of 10 orders of magnitude was observed with a limit of detection of 6.3 GCE/µL (95% CI, 2.6 to 139.4 GCE/µL). Conclusion: The plasmid construct is available for YFV molecular test validation on clinical, entomological, and epizootic samples.Minciencias Instituto Nacional de Salud Universidad Cooperativa de Colombia Universidad de Antioquia Universidad Nacional de Colombia5 páginasapplication/pdfengUniversidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Medicina, Santa MartaExternohttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://purl.org/coar/access_right/c_abf2Usefulness of an in vitro-transcribed RNA control for the detection and quantification of Yellow fever virus through real-time reverse transcription-polymerase chain reactionArtículohttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion10465453153Infectious Diseases NowMutebi J-P, Wang H, Li L, Bryant JE, Barrett ADT. Phylogenetic and Evolutionary Relationships among Yellow Fever Virus Isolates in Africa. J Virol 2001;75:6999–7008. https://doi.org/10.1128/JVI.75.15.6999-7008.2001.Bryant JE, Holmes EC, Barrett ADT. Out of Africa: A Molecular Perspective on the Introduction of Yellow Fever Virus into the Americas. PLoS Pathog 2007;3: e75. https://doi.org/10.1371/journal.ppat.0030075.Paules CI, Fauci AS. Yellow Fever — Once Again on the Radar Screen in the Americas. N Engl J Med 2017;376:1397–9. https://doi.org/10.1056/NEJMp1702172.Faria NR, Kraemer MUG, Hill SC, Goes de Jesus J, Aguiar RS, Iani FCM, et al. Genomic and epidemiological monitoring of yellow fever virus transmission potential. Science (80-) 2018;361:894–9. https://doi.org/10.1126/science.aat7115.Sacchetto L, Silva NIO, de Rezende IM, Arruda MS, Costa TA, de Mello ÉM, et al. Neighbor danger: Yellow fever virus epizootics in urban and urban-rural transition areas of Minas Gerais state, during 2017–2018 yellow fever outbreaks in Brazil. PLoS Negl Trop Dis 2020;14:e0008658. https://doi.org/10.1371/journal.pntd.0008658.Domingo C, Charrel RN, Schmidt-Chanasit J, Zeller H, Reusken C. Yellow fever in the diagnostics laboratory. Emerg Microbes Infect 2018;7:1–15. https://doi.org/10.1038/s41426-018-0128-8.Pan American Health Organization P. Real-time RT-PCR (TaqManTM) protocol -Yellow fever virus (YFV) 2018:1. https://www.paho.org/en/documents/realtime-rt-pcr-taqmantm-protocol-yellow-fever-virus-yfv (accessed May 17, 2022).Organización Panamericana de la Salud OPS. Recomendaciones para la detección y el diagnóstico por laboratorio de infecciones por arbovirus en la Región de las Américas. Washington DC: Pan American Health Organization; 2022. https://doi.org/10.37774/9789275325872.Domingo C, Patel P, Yillah J, Weidmann M, Méndez JA, Nakouné ER, et al. Advanced Yellow Fever Virus Genome Detection in Point-of-Care Facilities and Reference Laboratories. J Clin Microbiol 2012;50:4054–60. https://doi.org/10.1128/JCM.01799-12.Álvarez-Díaz DA, Quintero PA, Peláez-Carvajal D, Ajami NJ, Usme-Ciro JA. Novel pan-serotype control RNA for dengue virus typing through real-time reverse transcription-polymerase chain reaction. 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