Micropatterned silica thin films with nanohydroxyapatite micro-aggregates for guided tissue regeneration

Surface modification of biomaterials has been shown to improve the biological response to dental implants. The ability to create a controlled micro-texture on the implant via additive surface modification techniques with bioactive nanohydroxyapatite (nanoHA) may positively influence guided tissue regeneration. Objective: The main goal of this study was to produce micro-fabricated SiO 2 surfaces modified with nanohydroxyapatite particles and to characterize their influence on the biological response of Human Dental-Pulp Mesenchymal Stem Cells (hDP-MSCs) and Streptococcus mutans. Materials and methods: A combined methodology of sol-gel and soft-lithography was used to produce micropatterned SiO 2 thin films with different percentages of nanoHA micro-aggregates. The surfaces were characterized by SEM/EDS, FT-IR/ATR, AFM, XPS quantitative elemental percentage and contact angle measurements. Biological characterization was performed using hDP-MSCs cultures, while Streptococcus mutans was the selected microorganism to evaluate the bacterial adhesion on the thin films. Results: Micropatterned SiO 2 surfaces with 0%, 1% and 5% of nanoHA micro-aggregates were successfully produced using a combination of sol-gel and soft-lithography. These surfaces controlled the biological response, triggering alignment and oriented proliferation of hDP-MSCs and significant differences in the adhesion of S. mutans to the different surfaces. Significance: The micropatterned surfaces exhibited biocompatible behavior that induced an oriented adhesion and proliferation of hDP-MSCs while SiO 2 presented low bacterial adhesion. These results show that the combination of sol-gel with soft-lithography is a good approach to create micropatterned surfaces with bioactive nanoparticles for guided tissue regeneration. © 2012 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.

• Autores: Carvalho A.; Pelaez Vargas, Alejandro; Gallego-Perez D.; Grenho L.; Fernandes M.H.; De Aza A.H.; Ferraz M.P.; Hansford D.J.; Monteiro F.J.
• Tipo de recurso: Article of journal
• Fecha de publicación: 2012
• Institución: Universidad Cooperativa de Colombia
• Repositorio: Repositorio UCC
• OAI Identifier: oai:repository.ucc.edu.co:20.500.12494/41438
• Acceso en línea: https://doi.org/10.1016/j.humimm.2018.04.016; http://www.fipen.edu.br/hermes1/index.php/hermes1/article/view/481; https://hdl.handle.net/20.500.12494/41438
• Palabra clave: AFM; Bacterial adhesion; Bioactive nanoparticles; Biological characterization; Biological response; FTIR/ATR; Guided tissue regeneration; Mesenchymal stem cell; Micro patterning; Micro-texture; Microaggregates; Micropatterned; Micropatterned surface; Nano-HA; Nano-hydroxyapatite; S; mutans; SEM/EDS; Silica thin films; Soft-lithography; Streptococcus mutans; Surface modification techniques; Adhesion; Bacteria; Biological materials; Biomaterials; Cell culture; Contact angle; Dental prostheses; Flowcharting; Lithography; Silica; Sol-gel process; Sol-gels; Surfaces; Thin films; Aggregates; biomaterial; dental material; hydroxyapatite; nanomaterial; silicon dioxide; anisotropy; article; atomic force microscopy; bacterium adherence; biofilm; cell adhesion; cell count; cell proliferation; cell survival; chemistry; computer aided design; cytology; human; infrared spectroscopy; materials testing; mesenchymal stroma cell; methodology; phase transition; physiology; scanning electron microscopy; s
• Rights: closedAccess
• License: http://purl.org/coar/access_right/c_14cb