Human splenic macrophages as a model forin vitroinfection with Mycobacterium tuberculosis
Macrophages play an important role during Mycobacterium tuberculosis (MTB) infection. In humans most of the studies on MTB-macrophage interactions have been performed using circulating monocytes and monocyte-derived macrophages. However, little research has been performed on this interaction using t...
- Autores:
-
Henao Pérez, Julieta
Sánchez, Dulfary
Muñoz, Carlos H
Mejía, Natalia
Arias, Mauricio A
García, Luis Fernando
Barrera, Luis Fernando
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2023
- Institución:
- Universidad Cooperativa de Colombia
- Repositorio:
- Repositorio UCC
- Idioma:
- OAI Identifier:
- oai:repository.ucc.edu.co:20.500.12494/51392
- Acceso en línea:
- https://doi.org/10.1016/j.tube.2007.07.002
https://hdl.handle.net/20.500.12494/51392
- Palabra clave:
- M. Tuberculosis
Macrófagos esplénicos humanos
Citoquinas
M. Tuberculosis
Human splenic macrophages
Citoquines
- Rights
- closedAccess
- License
- http://purl.org/coar/access_right/c_14cb
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Human splenic macrophages as a model forin vitroinfection with Mycobacterium tuberculosis |
| title |
Human splenic macrophages as a model forin vitroinfection with Mycobacterium tuberculosis |
| spellingShingle |
Human splenic macrophages as a model forin vitroinfection with Mycobacterium tuberculosis M. Tuberculosis Macrófagos esplénicos humanos Citoquinas M. Tuberculosis Human splenic macrophages Citoquines |
| title_short |
Human splenic macrophages as a model forin vitroinfection with Mycobacterium tuberculosis |
| title_full |
Human splenic macrophages as a model forin vitroinfection with Mycobacterium tuberculosis |
| title_fullStr |
Human splenic macrophages as a model forin vitroinfection with Mycobacterium tuberculosis |
| title_full_unstemmed |
Human splenic macrophages as a model forin vitroinfection with Mycobacterium tuberculosis |
| title_sort |
Human splenic macrophages as a model forin vitroinfection with Mycobacterium tuberculosis |
| dc.creator.fl_str_mv |
Henao Pérez, Julieta Sánchez, Dulfary Muñoz, Carlos H Mejía, Natalia Arias, Mauricio A García, Luis Fernando Barrera, Luis Fernando |
| dc.contributor.advisor.none.fl_str_mv |
Henao Pérez, Julieta |
| dc.contributor.author.none.fl_str_mv |
Henao Pérez, Julieta Sánchez, Dulfary Muñoz, Carlos H Mejía, Natalia Arias, Mauricio A García, Luis Fernando Barrera, Luis Fernando |
| dc.subject.none.fl_str_mv |
M. Tuberculosis Macrófagos esplénicos humanos Citoquinas |
| topic |
M. Tuberculosis Macrófagos esplénicos humanos Citoquinas M. Tuberculosis Human splenic macrophages Citoquines |
| dc.subject.other.none.fl_str_mv |
M. Tuberculosis Human splenic macrophages Citoquines |
| description |
Macrophages play an important role during Mycobacterium tuberculosis (MTB) infection. In humans most of the studies on MTB-macrophage interactions have been performed using circulating monocytes and monocyte-derived macrophages. However, little research has been performed on this interaction using tissue macrophages. Herein, we used human splenic macrophages to characterize particular responses to MTB infection. Based on morphological, biochemical, and immunological markers, splenic adherent cells exhibit characteristics of tissue macrophages. They were able to efficiently phagocytose both live and heat-killed (h-k) MTB H37Rv. Upon infection with live, but not h-k MTB, an increase in secreted TNF-alpha was elicited. Splenic macrophages produced high basal levels of IL-10; however, infection with live or h-k MTB resulted in decrease IL-10 secretion. Both IL-12p40 and IL-12p70 basal levels were also decreased upon infection with live or h-k MTB; however, while the reduction for IL-12p40 levels was observed at earlier time points (4h) for both live and h-k MTB, infection with live MTB, but not h-k MTB, resulted in a time-dependent secretion of IL-12p40 at 24 and 48h after infection. IL-12p70 levels were completely reduced upon infection by either live or h-k MTB. These results support that human splenic macrophages may represent a potential useful model to study MTB-macrophage interactions in vitro. |
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2023 |
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2023-06-16T15:11:10Z |
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2023-06-16T15:11:10Z |
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2023-06-15 |
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Artículo |
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https://doi.org/10.1016/j.tube.2007.07.002 https://hdl.handle.net/20.500.12494/51392 |
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Henao, J., Sánchez, D., Muñoz, C. H., Mejía, N., Arias, M. A., García, L. F., & Barrera, L. F. (2007). Human splenic macrophages as a model for in vitro infection with Mycobacterium tuberculosis. [Articulo, Universidad Cooperativa de Colombia]. Repositorio Institucional Universidad Cooperativa de Colombia.https://repository.ucc.edu.co/handle/20.500.12494/51392 |
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https://doi.org/10.1016/j.tube.2007.07.002 https://hdl.handle.net/20.500.12494/51392 |
| identifier_str_mv |
Henao, J., Sánchez, D., Muñoz, C. H., Mejía, N., Arias, M. A., García, L. F., & Barrera, L. F. (2007). Human splenic macrophages as a model for in vitro infection with Mycobacterium tuberculosis. [Articulo, Universidad Cooperativa de Colombia]. Repositorio Institucional Universidad Cooperativa de Colombia.https://repository.ucc.edu.co/handle/20.500.12494/51392 |
| dc.relation.isversionof.none.fl_str_mv |
https://www.sciencedirect.com/science/article/abs/pii/S1472979207000704?via%3Dihub |
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Tuberculosis |
| dc.relation.references.none.fl_str_mv |
Vidal Pessolani MC, Marques MA, Reddy VM, Locht C, MenozziFD. Systemic dissemination in tuberculosis and leprosy: do mycobacterial adhesins play a role?Microbes Infect2003;5:677–84. Goyal M, Dhand R, Gupta N, Ganguly NK, Dang N. Monocyte/macrophage functions & humoral response in blood & bronch-oalveolar lavage fluid of pulmonary tuberculosis patients.Indian J Med Res1990;91:349–54. Kim SJ, Kim HI, Lee YH, Kim SK. Production of tumor necrosisfactor-alpha by alveolar macrophages from patients withpulmonary tuberculosis.J Korean Med Sci1991;6:45–53. Placido R, Mancino G, Amendola A, Mariani F, Vendetti S,Piacentini M, et al. Apoptosis of human monocytes/macro-phages inMycobacterium tuberculosisinfection.J Pathol1997;181:31–8. Korkusuz P, Dagdeviren A, Asan E. Immunophenotypic analysis ofhuman spleen compartments.Ann Anat2002;184:431–41. Schmalzl F, Braunsteiner H. The cytochemistry of monocytesand macrophages.Ser Haematol1970;3:93–131. Quesniaux V, Fremond C, Jacobs M, Parida S, Nicolle D,Yeremeev V, et al. Toll-like receptor pathways in the immuneresponses to mycobacteria.Microbes Infect2004;6:946–59. Takeda K, Akira S. Toll-like receptors in innate immunity.IntImmunol2005;17:1–14. Kraal G, Mebius R. New insights into the cell biology of themarginal zone of the spleen.Int Rev Cytol2006;250:175–215. Adams S, O’Neill DW, Bhardwaj N. Recent advances in dendriticcell biology.J Clin Immunol2005;25:177–88. Rossi M, Young JW. Human dendritic cells: potent antigen-presenting cells at the crossroads of innate and adaptiveimmunity.J Immunol2005;175:1373–81. Mebius RE, Kraal G. Structure and function of the spleen.NatRev Immunol2005;5:606–16. Flynn JL, Ernst JD. Immune responses in tuberculosis.Curr OpinImmunol2000;12:432–6. Dao DN, Kremer L, Guerardel Y, Molano A, Jacobs Jr. WR,Porcelli SA, et al.Mycobacterium tuberculosislipomannaninduces apoptosis and interleukin-12 production in macro-phages.Infect Immun2004;72:2067–74. Fulton SA, Johnsen JM, Wolf SF, Sieburth DS, Boom WH.Interleukin-12 production by human monocytes infected withMycobacterium tuberculosis: role of phagocytosis.InfectImmun1996;64:2523–31. Yang CS, Lee JS, Jung SB, Oh JH, Song CH, Kim HJ, et al.Differential regulation of interleukin-12 and tumour necrosisfactor-alpha by phosphatidylinositol 3-kinase and ERK 1/2pathways duringMycobacterium tuberculosisinfection.ClinExp Immunol2006;143:150–60. Blumenthal A, Ehlers S, Lauber J, Buer J, Lange C, Goldmann T,et al. The Wingless homolog WNT5A and its receptor Frizzled-5regulate inflammatory responses of human mononuclear cellsinduced by microbial stimulation.Blood2006;108:965–73. Giacomini E, Iona E, Ferroni L, Miettinen M, Fattorini L, OreficiG, et al. Infection of human macrophages and dendritic cellswithMycobacterium tuberculosisinduces a differential cyto-kine gene expression that modulates Tcell response.J Immunol2001;166:7033–41 Nigou J, Zelle-Rieser C, Gilleron M, Thurnher M, Puzo G.Mannosylated lipoarabinomannans inhibit IL-12 production byhuman dendritic cells: evidence for a negative signal deliveredthrough the mannose receptor.J Immunol2001;166:7477–85. Pathak SK, Basu S, Bhattacharyya A, Pathak S, Kundu M, Basu J.Mycobacterium tuberculosislipoarabinomannan-mediated IRAK-M induction negatively regulates toll-like receptor-depen-dent interleukin-12 p40 production in macrophages.J BiolChem2005;280:42794–800. Ehrt S, Schnappinger D, Bekiranov S, Drenkow J, Shi S, GingerasTR, et al. Reprogramming of the macrophage transcriptome inresponse to interferon-gamma andMycobacterium tuberculo-sis: signaling roles of nitric oxide synthase-2 and phagocyteoxidase.J Exp Med2001;194:1123–40. Beltan E, Horgen L, Rastogi N. Secretion of cytokines by humanmacrophages upon infection by pathogenic and non-pathogenicmycobacteria.Microb Pathog2000;28:313–8. Hasan Z, Shah BH, Mahmood A, Young DB, Hussain R. The effectof mycobacterial virulence and viability on MAP kinase signal-ling and TNF alpha production by human monocytes.Tubercu-losis2003;83:299–309. Surewicz K, Aung H, Kanost RA, Jones L, Hejal R, Toossi Z. Thedifferential interaction of p38 MAP kinase and tumor necrosisfactor-alpha in human alveolar macrophages and monocytesinduced byMycobacterium tuberculois.Cell Immunol2004;228:34–41. Verreck FA, de Boer T, Langenberg DM, Hoeve MA, Kramer M,Vaisberg E, Kastelein R, Kolk A, de Waal-Malefyt T, OttenhoffTH. Human IL-23-producing type 1 macrophages promote but IL-10-producing type 2 macrophages subvert immunity to myco-bacteria.PNAS2004;101:4560–5. Porcheray F, Viaud S, Rimaniol AC, Leone C, Samah B,Dereuddre-Bosquet N, et al. Macrophage activation switching:an asset for the resolution of inflammation.Clin Exp Immunol2005;142:481–9. Buckley PJ, Smith MR, Braverman MF, Dickson SA. Human spleencontains phenotypic subsets of macrophages and dendritic cellsthat occupy discrete microanatomic locations.Am J Pathol1987;128:505–20. Flynn JL, Chan J. Immune evasion byMycobacterium tubercu-losis: living with the enemy.Curr Opin Immunol2003;15:450–5. Sanchez MD, Garcia Y, Montes C, Paris SC, Rojas M, Barrera LF,et al. Functional and phenotypic changes in monocytes frompatients with tuberculosis are reversed with treatment.Microbes Infect2006;8:2400–92. |
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Henao Pérez, Julieta Henao Pérez, JulietaSánchez, DulfaryMuñoz, Carlos HMejía, NataliaArias, Mauricio AGarcía, Luis FernandoBarrera, Luis Fernando872023-06-16T15:11:10Z2023-06-16T15:11:10Z2023-06-15https://doi.org/10.1016/j.tube.2007.07.002https://hdl.handle.net/20.500.12494/51392Henao, J., Sánchez, D., Muñoz, C. H., Mejía, N., Arias, M. A., García, L. F., & Barrera, L. F. (2007). Human splenic macrophages as a model for in vitro infection with Mycobacterium tuberculosis. [Articulo, Universidad Cooperativa de Colombia]. Repositorio Institucional Universidad Cooperativa de Colombia.https://repository.ucc.edu.co/handle/20.500.12494/51392Macrophages play an important role during Mycobacterium tuberculosis (MTB) infection. In humans most of the studies on MTB-macrophage interactions have been performed using circulating monocytes and monocyte-derived macrophages. However, little research has been performed on this interaction using tissue macrophages. Herein, we used human splenic macrophages to characterize particular responses to MTB infection. Based on morphological, biochemical, and immunological markers, splenic adherent cells exhibit characteristics of tissue macrophages. They were able to efficiently phagocytose both live and heat-killed (h-k) MTB H37Rv. Upon infection with live, but not h-k MTB, an increase in secreted TNF-alpha was elicited. Splenic macrophages produced high basal levels of IL-10; however, infection with live or h-k MTB resulted in decrease IL-10 secretion. Both IL-12p40 and IL-12p70 basal levels were also decreased upon infection with live or h-k MTB; however, while the reduction for IL-12p40 levels was observed at earlier time points (4h) for both live and h-k MTB, infection with live MTB, but not h-k MTB, resulted in a time-dependent secretion of IL-12p40 at 24 and 48h after infection. IL-12p70 levels were completely reduced upon infection by either live or h-k MTB. These results support that human splenic macrophages may represent a potential useful model to study MTB-macrophage interactions in vitro.julieta.henaop@campusucc.edu.co509-517 p.Universidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Medicina, Medellín y EnvigadoMedicinaMedellínhttps://www.sciencedirect.com/science/article/abs/pii/S1472979207000704?via%3DihubTuberculosisVidal Pessolani MC, Marques MA, Reddy VM, Locht C, MenozziFD. Systemic dissemination in tuberculosis and leprosy: do mycobacterial adhesins play a role?Microbes Infect2003;5:677–84.Goyal M, Dhand R, Gupta N, Ganguly NK, Dang N. Monocyte/macrophage functions & humoral response in blood & bronch-oalveolar lavage fluid of pulmonary tuberculosis patients.Indian J Med Res1990;91:349–54.Kim SJ, Kim HI, Lee YH, Kim SK. Production of tumor necrosisfactor-alpha by alveolar macrophages from patients withpulmonary tuberculosis.J Korean Med Sci1991;6:45–53.Placido R, Mancino G, Amendola A, Mariani F, Vendetti S,Piacentini M, et al. Apoptosis of human monocytes/macro-phages inMycobacterium tuberculosisinfection.J Pathol1997;181:31–8.Korkusuz P, Dagdeviren A, Asan E. Immunophenotypic analysis ofhuman spleen compartments.Ann Anat2002;184:431–41.Schmalzl F, Braunsteiner H. The cytochemistry of monocytesand macrophages.Ser Haematol1970;3:93–131.Quesniaux V, Fremond C, Jacobs M, Parida S, Nicolle D,Yeremeev V, et al. Toll-like receptor pathways in the immuneresponses to mycobacteria.Microbes Infect2004;6:946–59.Takeda K, Akira S. Toll-like receptors in innate immunity.IntImmunol2005;17:1–14.Kraal G, Mebius R. New insights into the cell biology of themarginal zone of the spleen.Int Rev Cytol2006;250:175–215.Adams S, O’Neill DW, Bhardwaj N. Recent advances in dendriticcell biology.J Clin Immunol2005;25:177–88.Rossi M, Young JW. Human dendritic cells: potent antigen-presenting cells at the crossroads of innate and adaptiveimmunity.J Immunol2005;175:1373–81.Mebius RE, Kraal G. Structure and function of the spleen.NatRev Immunol2005;5:606–16.Flynn JL, Ernst JD. Immune responses in tuberculosis.Curr OpinImmunol2000;12:432–6.Dao DN, Kremer L, Guerardel Y, Molano A, Jacobs Jr. WR,Porcelli SA, et al.Mycobacterium tuberculosislipomannaninduces apoptosis and interleukin-12 production in macro-phages.Infect Immun2004;72:2067–74.Fulton SA, Johnsen JM, Wolf SF, Sieburth DS, Boom WH.Interleukin-12 production by human monocytes infected withMycobacterium tuberculosis: role of phagocytosis.InfectImmun1996;64:2523–31.Yang CS, Lee JS, Jung SB, Oh JH, Song CH, Kim HJ, et al.Differential regulation of interleukin-12 and tumour necrosisfactor-alpha by phosphatidylinositol 3-kinase and ERK 1/2pathways duringMycobacterium tuberculosisinfection.ClinExp Immunol2006;143:150–60.Blumenthal A, Ehlers S, Lauber J, Buer J, Lange C, Goldmann T,et al. The Wingless homolog WNT5A and its receptor Frizzled-5regulate inflammatory responses of human mononuclear cellsinduced by microbial stimulation.Blood2006;108:965–73.Giacomini E, Iona E, Ferroni L, Miettinen M, Fattorini L, OreficiG, et al. Infection of human macrophages and dendritic cellswithMycobacterium tuberculosisinduces a differential cyto-kine gene expression that modulates Tcell response.J Immunol2001;166:7033–41Nigou J, Zelle-Rieser C, Gilleron M, Thurnher M, Puzo G.Mannosylated lipoarabinomannans inhibit IL-12 production byhuman dendritic cells: evidence for a negative signal deliveredthrough the mannose receptor.J Immunol2001;166:7477–85.Pathak SK, Basu S, Bhattacharyya A, Pathak S, Kundu M, Basu J.Mycobacterium tuberculosislipoarabinomannan-mediated IRAK-M induction negatively regulates toll-like receptor-depen-dent interleukin-12 p40 production in macrophages.J BiolChem2005;280:42794–800.Ehrt S, Schnappinger D, Bekiranov S, Drenkow J, Shi S, GingerasTR, et al. Reprogramming of the macrophage transcriptome inresponse to interferon-gamma andMycobacterium tuberculo-sis: signaling roles of nitric oxide synthase-2 and phagocyteoxidase.J Exp Med2001;194:1123–40.Beltan E, Horgen L, Rastogi N. Secretion of cytokines by humanmacrophages upon infection by pathogenic and non-pathogenicmycobacteria.Microb Pathog2000;28:313–8.Hasan Z, Shah BH, Mahmood A, Young DB, Hussain R. The effectof mycobacterial virulence and viability on MAP kinase signal-ling and TNF alpha production by human monocytes.Tubercu-losis2003;83:299–309.Surewicz K, Aung H, Kanost RA, Jones L, Hejal R, Toossi Z. Thedifferential interaction of p38 MAP kinase and tumor necrosisfactor-alpha in human alveolar macrophages and monocytesinduced byMycobacterium tuberculois.Cell Immunol2004;228:34–41.Verreck FA, de Boer T, Langenberg DM, Hoeve MA, Kramer M,Vaisberg E, Kastelein R, Kolk A, de Waal-Malefyt T, OttenhoffTH. Human IL-23-producing type 1 macrophages promote but IL-10-producing type 2 macrophages subvert immunity to myco-bacteria.PNAS2004;101:4560–5.Porcheray F, Viaud S, Rimaniol AC, Leone C, Samah B,Dereuddre-Bosquet N, et al. Macrophage activation switching:an asset for the resolution of inflammation.Clin Exp Immunol2005;142:481–9.Buckley PJ, Smith MR, Braverman MF, Dickson SA. Human spleencontains phenotypic subsets of macrophages and dendritic cellsthat occupy discrete microanatomic locations.Am J Pathol1987;128:505–20.Flynn JL, Chan J. Immune evasion byMycobacterium tubercu-losis: living with the enemy.Curr Opin Immunol2003;15:450–5.Sanchez MD, Garcia Y, Montes C, Paris SC, Rojas M, Barrera LF,et al. Functional and phenotypic changes in monocytes frompatients with tuberculosis are reversed with treatment.Microbes Infect2006;8:2400–92.M. TuberculosisMacrófagos esplénicos humanosCitoquinasM. 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