An overview of the trypanosomatid (kinetoplastida: trypanosomatidae) parasites infecting several mammal species in Colombia
Antecedentes: Los tripanosomátidos se encuentran entre los parásitos más críticos para la salud pública debido a su impacto en humanos, sanidad animal y vegetal. Las enfermedades asociadas con estos patógenos se manifiestan principalmente en poblaciones pobres y vulnerables. ciones, donde factores s...
- Autores:
-
Castillo Castañeda, Adriana C.
Patiño, Luz H.
Zuñiga, Maria Fernanda
Cantillo Barraza, Omar
Ayala, Martha S.
Segura, Maryi
Bautista, Jessica
Urbano, Plutarco
Jaimes Dueñez, Jeiczon Elim
Ramírez, Juan David
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2022
- Institución:
- Universidad Cooperativa de Colombia
- Repositorio:
- Repositorio UCC
- Idioma:
- OAI Identifier:
- oai:repository.ucc.edu.co:20.500.12494/52717
- Acceso en línea:
- https://doi.org/10.1186/s13071-022-05595-y
https://hdl.handle.net/20.500.12494/52717
- Palabra clave:
- NGS basada en amplicones
Mamíferos
Tripanosomátidos
Coinfección
Diversidad
Amplicon-based NGS
Sanger Mammals
Trypanosomatids
Coinfection
Diversity
- Rights
- openAccess
- License
- NINGUNA
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oai:repository.ucc.edu.co:20.500.12494/52717 |
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COOPER2 |
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Repositorio UCC |
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| dc.title.none.fl_str_mv |
An overview of the trypanosomatid (kinetoplastida: trypanosomatidae) parasites infecting several mammal species in Colombia |
| title |
An overview of the trypanosomatid (kinetoplastida: trypanosomatidae) parasites infecting several mammal species in Colombia |
| spellingShingle |
An overview of the trypanosomatid (kinetoplastida: trypanosomatidae) parasites infecting several mammal species in Colombia NGS basada en amplicones Mamíferos Tripanosomátidos Coinfección Diversidad Amplicon-based NGS Sanger Mammals Trypanosomatids Coinfection Diversity |
| title_short |
An overview of the trypanosomatid (kinetoplastida: trypanosomatidae) parasites infecting several mammal species in Colombia |
| title_full |
An overview of the trypanosomatid (kinetoplastida: trypanosomatidae) parasites infecting several mammal species in Colombia |
| title_fullStr |
An overview of the trypanosomatid (kinetoplastida: trypanosomatidae) parasites infecting several mammal species in Colombia |
| title_full_unstemmed |
An overview of the trypanosomatid (kinetoplastida: trypanosomatidae) parasites infecting several mammal species in Colombia |
| title_sort |
An overview of the trypanosomatid (kinetoplastida: trypanosomatidae) parasites infecting several mammal species in Colombia |
| dc.creator.fl_str_mv |
Castillo Castañeda, Adriana C. Patiño, Luz H. Zuñiga, Maria Fernanda Cantillo Barraza, Omar Ayala, Martha S. Segura, Maryi Bautista, Jessica Urbano, Plutarco Jaimes Dueñez, Jeiczon Elim Ramírez, Juan David |
| dc.contributor.author.none.fl_str_mv |
Castillo Castañeda, Adriana C. Patiño, Luz H. Zuñiga, Maria Fernanda Cantillo Barraza, Omar Ayala, Martha S. Segura, Maryi Bautista, Jessica Urbano, Plutarco Jaimes Dueñez, Jeiczon Elim Ramírez, Juan David |
| dc.subject.none.fl_str_mv |
NGS basada en amplicones Mamíferos Tripanosomátidos Coinfección Diversidad |
| topic |
NGS basada en amplicones Mamíferos Tripanosomátidos Coinfección Diversidad Amplicon-based NGS Sanger Mammals Trypanosomatids Coinfection Diversity |
| dc.subject.other.none.fl_str_mv |
Amplicon-based NGS Sanger Mammals Trypanosomatids Coinfection Diversity |
| description |
Antecedentes: Los tripanosomátidos se encuentran entre los parásitos más críticos para la salud pública debido a su impacto en humanos, sanidad animal y vegetal. Las enfermedades asociadas con estos patógenos se manifiestan principalmente en poblaciones pobres y vulnerables. ciones, donde factores sociales, ambientales y biológicos modulan la incidencia de casos y la distribución geográfica. Métodos: Utilizamos Sanger y secuenciación de próxima generación (NGS) basada en amplicones en muestras de diferentes mamíferos para identificar infecciones por tripanosomátidos en varios departamentos de Colombia. Un total de 174 muestras de ADN (18 humanos, 83 perros y 73 mamíferos salvajes) fueron analizados mediante PCR convencional utilizando un fragmento del choque térmico gen de la proteína 70 (Hsp70) y Sanger secuenciaron las muestras positivas. Se enviaron veintisiete muestras para NGS basada en amplicones utilizando el mismo fragmento de gen. Los datos obtenidos se utilizaron para realizar análisis de diversidad. Resultados: Ciento trece muestras resultaron positivas para PCR por fragmento Hsp70; estos correspondieron al 22,1% Leishmania spp., 18,6% L. amazonensis, 9,7% L. braziliensis, 14,2% L. infantum, 8% L. panamensis y 27,4% Trypanosoma cruzi. La comparación de las especies identificadas mediante las dos tecnologías de secuenciación utilizadas dio como resultado un 97% de concordancia. ance. Los índices de diversidad alfa y beta fueron significativos, principalmente para perros; hubo un interesante índice de coinfección eventos en las muestras analizadas: diferentes especies de Leishmania y la presencia simultánea de T. cruzi e incluso T. rangeli en una de las muestras analizadas. Además, se observó una baja presencia de L. braziliensis en muestras de especies silvestres. mamíferos. Curiosamente, hasta donde sabemos, este es el primer informe de detección de Leishmania en Hydrochaeris hydrochaeris.(capibara) en Colombia |
| publishDate |
2022 |
| dc.date.issued.none.fl_str_mv |
2022-12-16 |
| dc.date.accessioned.none.fl_str_mv |
2023-09-19T18:35:09Z |
| dc.date.available.none.fl_str_mv |
2023-09-19T18:35:09Z |
| dc.type.none.fl_str_mv |
Artículos Científicos |
| dc.type.coar.none.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
| dc.type.coarversion.none.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.driver.none.fl_str_mv |
info:eu-repo/semantics/article |
| dc.type.version.none.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| format |
http://purl.org/coar/resource_type/c_2df8fbb1 |
| status_str |
publishedVersion |
| dc.identifier.uri.none.fl_str_mv |
https://doi.org/10.1186/s13071-022-05595-y https://hdl.handle.net/20.500.12494/52717 |
| dc.identifier.bibliographicCitation.none.fl_str_mv |
Castillo-Castañeda, A.C., Patiño, L.H., Zuñiga, M.F. et al. An overview of the trypanosomatid (Kinetoplastida: Trypanosomatidae) parasites infecting several mammal species in Colombia. Parasites Vectors 15, 471 (2022). https://doi.org/10.1186/s13071-022-05595-y |
| url |
https://doi.org/10.1186/s13071-022-05595-y https://hdl.handle.net/20.500.12494/52717 |
| identifier_str_mv |
Castillo-Castañeda, A.C., Patiño, L.H., Zuñiga, M.F. et al. An overview of the trypanosomatid (Kinetoplastida: Trypanosomatidae) parasites infecting several mammal species in Colombia. Parasites Vectors 15, 471 (2022). https://doi.org/10.1186/s13071-022-05595-y |
| dc.relation.isversionof.none.fl_str_mv |
https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-022-05595-y#citeas |
| dc.relation.references.none.fl_str_mv |
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Medinger R, Nolte V, Pandey RV, Jost S, Ottenwälder B, Schlöt‑ terer C, et al. diversity in a hidden world: potential and limitation of Castillo‑Castañeda et al. Parasites & Vectors (2022) 15:471 Page 13 of 13 • fast, convenient online submission • thorough peer review by experienced researchers in your field • rapid publication on acceptance • support for research data, including large and complex data types • gold Open Access which fosters wider collaboration and increased citations • maximum visibility for your research: over 100M website views per year At BMC, research is always in progress. Learn more biomedcentral.com/submissions Ready to submit y submit your research ? Choose BMC and benefit from: next -generation sequencing for surveys of molecular diversity of eukaryotic microorganisms. Mol Ecol. 2010;19:32–40. Rodrigues DC, Silva R, Rondinelli E, Ürményi TP. Trypanosoma cruzi: modulation of HSP70 mRNA stability by untranslated regions during heat shock. Exp Parasitol. 2010;126:245–53. Hernández C, Salazar C, Brochero H, Teherán A, Buitrago LS, Vera M, et al. Untangling the transmission dynamics of primary and secondary vectors of Trypanosoma cruzi in Colombia: parasite infection, feeding sources and discrete typing units. Parasit Vectors. 2016;9:620 Filgueira CPB, Moreira OC, Cantanhêde LM, de Farias HMT, Porrozzi R, Britto C, et al. Comparison and clinical validation of qPCR assays target ‑ ing Leishmania 18S rDNA and HSP70 genes in patients with American tegumentary leishmaniasis. PLoS Negl Trop Dis. 2020;14:e0008750. |
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Castillo Castañeda, Adriana C. Patiño, Luz H.Zuñiga, Maria FernandaCantillo Barraza, OmarAyala, Martha S.Segura, MaryiBautista, JessicaUrbano, PlutarcoJaimes Dueñez, Jeiczon Elim Ramírez, Juan David2023-09-19T18:35:09Z2023-09-19T18:35:09Z2022-12-16https://doi.org/10.1186/s13071-022-05595-yhttps://hdl.handle.net/20.500.12494/52717Castillo-Castañeda, A.C., Patiño, L.H., Zuñiga, M.F. et al. An overview of the trypanosomatid (Kinetoplastida: Trypanosomatidae) parasites infecting several mammal species in Colombia. Parasites Vectors 15, 471 (2022). https://doi.org/10.1186/s13071-022-05595-yAntecedentes: Los tripanosomátidos se encuentran entre los parásitos más críticos para la salud pública debido a su impacto en humanos, sanidad animal y vegetal. Las enfermedades asociadas con estos patógenos se manifiestan principalmente en poblaciones pobres y vulnerables. ciones, donde factores sociales, ambientales y biológicos modulan la incidencia de casos y la distribución geográfica. Métodos: Utilizamos Sanger y secuenciación de próxima generación (NGS) basada en amplicones en muestras de diferentes mamíferos para identificar infecciones por tripanosomátidos en varios departamentos de Colombia. Un total de 174 muestras de ADN (18 humanos, 83 perros y 73 mamíferos salvajes) fueron analizados mediante PCR convencional utilizando un fragmento del choque térmico gen de la proteína 70 (Hsp70) y Sanger secuenciaron las muestras positivas. Se enviaron veintisiete muestras para NGS basada en amplicones utilizando el mismo fragmento de gen. Los datos obtenidos se utilizaron para realizar análisis de diversidad. Resultados: Ciento trece muestras resultaron positivas para PCR por fragmento Hsp70; estos correspondieron al 22,1% Leishmania spp., 18,6% L. amazonensis, 9,7% L. braziliensis, 14,2% L. infantum, 8% L. panamensis y 27,4% Trypanosoma cruzi. La comparación de las especies identificadas mediante las dos tecnologías de secuenciación utilizadas dio como resultado un 97% de concordancia. ance. Los índices de diversidad alfa y beta fueron significativos, principalmente para perros; hubo un interesante índice de coinfección eventos en las muestras analizadas: diferentes especies de Leishmania y la presencia simultánea de T. cruzi e incluso T. rangeli en una de las muestras analizadas. Además, se observó una baja presencia de L. braziliensis en muestras de especies silvestres. mamíferos. Curiosamente, hasta donde sabemos, este es el primer informe de detección de Leishmania en Hydrochaeris hydrochaeris.(capibara) en ColombiaBackground: Trypanosomatids are among the most critical parasites for public health due to their impact on human, animal, and plant health. Diseases associated with these pathogens manifest mainly in poor and vulnerable popula‑ tions, where social, environmental, and biological factors modulate the case incidence and geographical distribution. Methods: We used Sanger and amplicon-based next-generation sequencing (NGS) in samples from diferent mammals to identify trypanosomatid infections in several departments in Colombia. A total of 174 DNA samples (18 humans, 83 dogs, and 73 wild mammals) were analyzed by conventional PCR using a fragment of the heat shock protein 70 (Hsp70) gene and Sanger sequenced the positive samples. Twenty-seven samples were sent for ampliconbased NGS using the same gene fragment. Data obtained were used to perform diversity analyses. Results: One hundred and thirteen samples were positive for PCR by Hsp70 fragment; these corresponded to 22.1% Leishmania spp., 18.6% L. amazonensis, 9.7% L. braziliensis, 14.2% L. infantum, 8% L. panamensis, and 27.4% Trypanosoma cruzi. Comparison of the identifed species by the two sequencing technologies used resulted in 97% concord‑ ance. Alpha and beta diversity indices were signifcant, mainly for dogs; there was an interesting index of coinfection events in the analyzed samples: diferent Leishmania species and the simultaneous presence of T. cruzi and even T. rangeli in one of the samples analyzed. Moreover, a low presence of L. braziliensis was observed in samples from wild mammals. Interestingly, to our knowledge, this is the frst report of Leishmania detection in Hydrochaeris hydrochaeris (capybara) in Colombia.juand.ramirez@urosario.edu.coJeiczon.jaimes@campusucc.edu.co13 p.Universidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Medicina Veterinaría y Zootecnia, BucaramangaMedicina veterinaria y zootecniaBucaramangahttps://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-022-05595-y#citeasRodrigues JCF, Godinho JLP, de Souza W. 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Leishmania-host interactions—an epigenetic paradigm. Front Immunol. 2019;10:492Alemayehu B, Alemayehu M. Leishmaniasis: A review on parasite, vector and reservoir host. Health Sci J. 2017. http://www.hsj.gr/medicine/leish maniasis-a-review-on-parasite-vector-and-reservoir-host.php?aid= 20131. Accessed on 21 Oct 2019de Castro Neto AL, da Silveira JF, Mortara RA. Comparative analysis of virulence mechanisms of trypanosomatids pathogenic to humans. Front Cell Infect Microbiol. 2021;11:669079.Elmahallawy EK, Alkhaldi AAM, Saleh AA. Host immune response against leishmaniasis and parasite persistence strategies: a review and assessment of recent research. Biomed Pharmacother. 2021;139:111671.Ferro C, López M, Fuya P, Lugo L, Cordovez JM, González C. Spatial Distribution of Sand Fly Vectors and Eco-Epidemiology of Cutaneous Leishmaniasis Transmission in Colombia. PLOS ONE. 2015;10:e0139391.González C, Cabrera OL, Munstermann LE, Ferro C. 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PLoS Negl Trop Dis. 2020;14:e0008750.NGS basada en ampliconesMamíferosTripanosomátidosCoinfecciónDiversidadAmplicon-based NGSSanger MammalsTrypanosomatidsCoinfectionDiversityAn overview of the trypanosomatid (kinetoplastida: trypanosomatidae) parasites infecting several mammal species in ColombiaArtículos Científicoshttp://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionNINGUNAinfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2PublicationLICENSElicense.txtlicense.txttext/plain; charset=utf-84334https://repository.ucc.edu.co/bitstreams/63a4faac-a7f8-4268-9a9f-f10909ac4bd5/download3bce4f7ab09dfc588f126e1e36e98a45MD5120.500.12494/52717oai:repository.ucc.edu.co:20.500.12494/527172024-08-09 12:27:26.317metadata.onlyhttps://repository.ucc.edu.coRepositorio Institucional Universidad Cooperativa de 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