5'/3' RACE method for sequencing the 5' and 3' untranslated regions of Zika virus
The spread of Zika virus (ZIKV) from the African continent to the Americas promoted its molecular evolution, as reflected by mutations in its RNA genome. Most of the ZIKV genome sequences in the GenBank database have incomplete 5' and 3' UTR sequences, reflecting the deficiency of whole-ge...
- Autores:
-
Álvarez Díaz, Diego Alejandro
Usme Ciro, José Aldemar
Corchuelo, Sheryll
Naizaque, Julián Ricardo
Rivera, Jorge Alonso
Castiblanco Martínez, Hernán Darío
Torres Fernández, Orlando
Rengifo, Aura Caterine
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2023
- Institución:
- Universidad Cooperativa de Colombia
- Repositorio:
- Repositorio UCC
- Idioma:
- eng
- OAI Identifier:
- oai:repository.ucc.edu.co:20.500.12494/55526
- Acceso en línea:
- https://hdl.handle.net/20.500.12494/55526
- Palabra clave:
- 610 - Medicina y salud
Zika virus
RNA genome
ZIKV genome
UTR sequences
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by-nc-nd/4.0/
| Summary: | The spread of Zika virus (ZIKV) from the African continent to the Americas promoted its molecular evolution, as reflected by mutations in its RNA genome. Most of the ZIKV genome sequences in the GenBank database have incomplete 5' and 3' UTR sequences, reflecting the deficiency of whole-genome sequencing technologies to resolve the sequences of the genome ends. We modified a protocol for rapid amplification of cDNA ends (RACE) to determine the complete sequences of the 5' and 3' UTRs of a previously reported ZIKV isolate (GenBank no. MH544701.1). This strategy is useful for determining 5' and 3' UTR sequences of ZIKV isolates and will be useful for comparative genomics applications. |
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