High-Throughput sequencing reveals circulating miRNAs as potential biomarkers of kidney damage in patients with systemic lupus erythematosus
Estudio exploratorio que busca identificar mirnas expresados diferenciamente en con lupus eritematoso sistemico que presentan alteraciones renales.
- Autores:
-
Navarro Quiroz, Elkin Antonio
Pacheco Lugo, Lisandro
Lorenzi, Hernan
Díaz Olmos, Yirys
Almendrales, Lisneth
Rico, Edwin
Navarro Quiroz, Roberto Carlos
España Puccini, Pierine
Iglesias, Antonio
Egea Bermejo, Eduardo Alberto
Aroca Martínez, Gustavo
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2016
- Institución:
- Universidad Cooperativa de Colombia
- Repositorio:
- Repositorio UCC
- Idioma:
- OAI Identifier:
- oai:repository.ucc.edu.co:20.500.12494/1306
- Acceso en línea:
- https://hdl.handle.net/20.500.12494/1306
- Palabra clave:
- Systemic Lupus Erythematosus
miRNAs
- Rights
- openAccess
- License
- Licencia CC
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High-Throughput sequencing reveals circulating miRNAs as potential biomarkers of kidney damage in patients with systemic lupus erythematosus |
| title |
High-Throughput sequencing reveals circulating miRNAs as potential biomarkers of kidney damage in patients with systemic lupus erythematosus |
| spellingShingle |
High-Throughput sequencing reveals circulating miRNAs as potential biomarkers of kidney damage in patients with systemic lupus erythematosus Systemic Lupus Erythematosus miRNAs |
| title_short |
High-Throughput sequencing reveals circulating miRNAs as potential biomarkers of kidney damage in patients with systemic lupus erythematosus |
| title_full |
High-Throughput sequencing reveals circulating miRNAs as potential biomarkers of kidney damage in patients with systemic lupus erythematosus |
| title_fullStr |
High-Throughput sequencing reveals circulating miRNAs as potential biomarkers of kidney damage in patients with systemic lupus erythematosus |
| title_full_unstemmed |
High-Throughput sequencing reveals circulating miRNAs as potential biomarkers of kidney damage in patients with systemic lupus erythematosus |
| title_sort |
High-Throughput sequencing reveals circulating miRNAs as potential biomarkers of kidney damage in patients with systemic lupus erythematosus |
| dc.creator.fl_str_mv |
Navarro Quiroz, Elkin Antonio Pacheco Lugo, Lisandro Lorenzi, Hernan Díaz Olmos, Yirys Almendrales, Lisneth Rico, Edwin Navarro Quiroz, Roberto Carlos España Puccini, Pierine Iglesias, Antonio Egea Bermejo, Eduardo Alberto Aroca Martínez, Gustavo |
| dc.contributor.author.none.fl_str_mv |
Navarro Quiroz, Elkin Antonio Pacheco Lugo, Lisandro Lorenzi, Hernan Díaz Olmos, Yirys Almendrales, Lisneth Rico, Edwin Navarro Quiroz, Roberto Carlos España Puccini, Pierine Iglesias, Antonio Egea Bermejo, Eduardo Alberto Aroca Martínez, Gustavo |
| dc.subject.spa.fl_str_mv |
Systemic Lupus Erythematosus miRNAs |
| topic |
Systemic Lupus Erythematosus miRNAs |
| description |
Estudio exploratorio que busca identificar mirnas expresados diferenciamente en con lupus eritematoso sistemico que presentan alteraciones renales. |
| publishDate |
2016 |
| dc.date.issued.none.fl_str_mv |
2016-11-11 |
| dc.date.accessioned.none.fl_str_mv |
2017-08-23T17:56:01Z |
| dc.date.available.none.fl_str_mv |
2017-08-23T17:56:01Z 2018-01-21 |
| dc.type.none.fl_str_mv |
Artículo |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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https://hdl.handle.net/20.500.12494/1306 |
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Navarro Quiroz, E., Pacheco Lugo, L., Lorenzi, H., Díaz Olmos, Y., Almendrales, L., Rico, E., Navarro, R., España Puccini, P., Iglesias, A., Egea, E., & Aroca. (2016) High-Throughput Sequencing Reveals Circulating miRNAs as Potential Biomarkers of Kidney Damage in Patients with Systemic Lupus Erythematosus. PLoS ONE 11(11): e0166202. https://doi.org/10.1371/journal.pone.0166202 |
| url |
https://hdl.handle.net/20.500.12494/1306 |
| identifier_str_mv |
Navarro Quiroz, E., Pacheco Lugo, L., Lorenzi, H., Díaz Olmos, Y., Almendrales, L., Rico, E., Navarro, R., España Puccini, P., Iglesias, A., Egea, E., & Aroca. (2016) High-Throughput Sequencing Reveals Circulating miRNAs as Potential Biomarkers of Kidney Damage in Patients with Systemic Lupus Erythematosus. PLoS ONE 11(11): e0166202. https://doi.org/10.1371/journal.pone.0166202 |
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0166202 |
| dc.rights.cc.none.fl_str_mv |
Licencia CC |
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info:eu-repo/semantics/openAccess |
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http://purl.org/coar/access_right/c_abf2 |
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Licencia CC http://purl.org/coar/access_right/c_abf2 |
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openAccess |
| dc.publisher.spa.fl_str_mv |
Universidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Programa de Medicina, Santa Marta, Colombia, 00000 |
| dc.publisher.program.spa.fl_str_mv |
Medicina |
| dc.publisher.place.spa.fl_str_mv |
Santa Marta |
| institution |
Universidad Cooperativa de Colombia |
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Navarro Quiroz, Elkin AntonioPacheco Lugo, LisandroLorenzi, HernanDíaz Olmos, YirysAlmendrales, LisnethRico, EdwinNavarro Quiroz, Roberto CarlosEspaña Puccini, PierineIglesias, AntonioEgea Bermejo, Eduardo AlbertoAroca Martínez, Gustavo2017-08-23T17:56:01Z2018-01-212017-08-23T17:56:01Z2016-11-11https://hdl.handle.net/20.500.12494/1306Navarro Quiroz, E., Pacheco Lugo, L., Lorenzi, H., Díaz Olmos, Y., Almendrales, L., Rico, E., Navarro, R., España Puccini, P., Iglesias, A., Egea, E., & Aroca. (2016) High-Throughput Sequencing Reveals Circulating miRNAs as Potential Biomarkers of Kidney Damage in Patients with Systemic Lupus Erythematosus. PLoS ONE 11(11): e0166202. https://doi.org/10.1371/journal.pone.0166202Estudio exploratorio que busca identificar mirnas expresados diferenciamente en con lupus eritematoso sistemico que presentan alteraciones renales.Renal involvement is one of the most severe manifestations of systemic lupus erythematosus (SLE). Renal biopsy is the gold standard when it comes to knowing whether a patient has lupus nephritis, and the degree of renal disease present. However, the biopsy has various complications, bleeding being the most common. Therefore, the development of alternative, non-invasive diagnostic tests for kidney disease in patients with SLE is a priority. Micro RNAs (miRNAs) are differentially expressed in various tissues, and changes in their expression have been associated with several pathological processes. The aim of this study was to identify changes in the abundance of miRNAs in plasma samples from patients with lupus nephritis that could potentially allow the diagnosis of renal damage in SLE patients. This is an observational case-control cross-sectional study, in which we characterized the differential abundance profiles of miRNAs among patients with different degrees of lupus compared with SLE patients without renal involvement and healthy control individuals. We found 89 miRNAs with changes in their abundance between lupus nephritis patients and healthy controls, and 17 miRNAs that showed significant variations between SLE patients with or without renal involvement. Validation for qPCR of a group of miRNAs on additional samples from lupus patients with or without nephritis, and from healthy individuals, showed that five miRNAs presented an average detection sensitivity of 97%, a specificity of 70.3%, a positive predictive value of 82.5%, a negative predictive value of 96% and a diagnosis efficiency of 87.9%. These results strongly suggest that miR-221-5p, miR-380-3p, miR-556-5p, miR-758-3p and miR-3074-3p are potential diagnostic biomarkers of lupus nephritis in patients with SLE. The observed differential pattern of miRNA abundance may have functional implications in the pathophysiology of SLE renal damage.Abstract -- Introduction -- Materials and Methods -- Results -- Discussion -- Supporting Information -- Acknowledgments -- Author Contributions -- References -- Reader Comments (0) -- Media Coverage (0) -- Figures.enavarro26@unisimonbolivar.edu.coUniversidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Programa de Medicina, Santa Marta, Colombia, 00000MedicinaSanta Martahttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0166202Systemic Lupus ErythematosusmiRNAsHigh-Throughput sequencing reveals circulating miRNAs as potential biomarkers of kidney damage in patients with systemic lupus erythematosusArtículohttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionLicencia CCinfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2PublicationORIGINALHigh-Throughput Sequencing Reveals Circulating miRNAs as Potential Biomarkers of Kidney Damage in Patients with Systemic Lupus Erythematosus.PDFHigh-Throughput Sequencing Reveals Circulating miRNAs as Potential Biomarkers of Kidney Damage in Patients with Systemic Lupus Erythematosus.PDFapplication/pdf1767440https://repository.ucc.edu.co/bitstreams/f15c356b-0516-4ba0-a5af-6ca04a11575e/download653c5196fda5a1ca38d6710358999f03MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-84553https://repository.ucc.edu.co/bitstreams/fa53a4e4-847b-4de7-ab25-89722921abda/downloadcb8f4d9b72d863ad6737f22af442cc82MD52TEXTHigh-Throughput Sequencing Reveals Circulating miRNAs as Potential Biomarkers of Kidney Damage in Patients with Systemic Lupus Erythematosus.PDF.txtHigh-Throughput Sequencing Reveals Circulating miRNAs as Potential Biomarkers of Kidney Damage in Patients with Systemic Lupus Erythematosus.PDF.txtExtracted texttext/plain35078https://repository.ucc.edu.co/bitstreams/745a309d-9837-4fc9-82f3-176a2c464273/download8cd3b3e14341f7b4b9a70a176c8031acMD53THUMBNAILHigh-Throughput Sequencing Reveals Circulating miRNAs as Potential Biomarkers of Kidney Damage in Patients with Systemic Lupus Erythematosus.PDF.jpgHigh-Throughput Sequencing Reveals Circulating miRNAs as Potential Biomarkers of Kidney Damage in Patients with Systemic Lupus Erythematosus.PDF.jpgIM Thumbnailimage/jpeg8272https://repository.ucc.edu.co/bitstreams/ad53066b-394e-406b-856c-692754196e18/download7fb73ad93a2cd8699e7e364ab3559b38MD5420.500.12494/1306oai:repository.ucc.edu.co:20.500.12494/13062024-08-10 22:45:06.348open.accesshttps://repository.ucc.edu.coRepositorio Institucional Universidad Cooperativa de 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