Identifying different transcribed proteins in the newly described Theraphosidae Pamphobeteus verdolaga

Theraphosidae spider venoms are well known for possess a complex mixture of protein and non-protein compounds in their venom. The objective of this study was to report and identify different proteins translated from the venom gland DNA information of the recently described Theraphosidae spider Pamph...

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Autores:
Estrada-Gómez, S.
Vargas Muñoz, Leidy Johana
Saldarriaga Córdoba, M.
Cifuentes, Y.
Perafan, C.
Tipo de recurso:
Article of journal
Fecha de publicación:
2023
Institución:
Universidad Cooperativa de Colombia
Repositorio:
Repositorio UCC
Idioma:
OAI Identifier:
oai:repository.ucc.edu.co:20.500.12494/49523
Acceso en línea:
https://doi.org/10.1016/j.toxicon.2017.02.004
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85013135314&doi=10.1016%2fj.toxicon.2017.02.004&partnerID=40&md5=ff2e53344a833f0e160fc75591c81b02
https://hdl.handle.net/20.500.12494/49523
Palabra clave:
PAMPHOBETEUS VERDOLAGA
PROTEOMIC
THERAPHOSIDAE
TRANSCRIPTOMIC
Rights
openAccess
License
http://purl.org/coar/access_right/c_abf2
id COOPER2_c90b01a8749ab5d31eab572d3166d22b
oai_identifier_str oai:repository.ucc.edu.co:20.500.12494/49523
network_acronym_str COOPER2
network_name_str Repositorio UCC
repository_id_str
spelling Estrada-Gómez, S.Vargas Muñoz, Leidy JohanaSaldarriaga Córdoba, M.Cifuentes, Y.Perafan, C.2023-05-24T16:21:29Z2023-05-24T16:21:29Z01/04/2017https://doi.org/10.1016/j.toxicon.2017.02.004https://www.scopus.com/inward/record.uri?eid=2-s2.0-85013135314&doi=10.1016%2fj.toxicon.2017.02.004&partnerID=40&md5=ff2e53344a833f0e160fc75591c81b0200410101https://hdl.handle.net/20.500.12494/49523Estrada-Gómez S.,Vargas Muñoz Leidy johana,Saldarriaga-Córdoba M.,Cifuentes Y.,Perafan C..Identifying different transcribed proteins in the newly described Theraphosidae Pamphobeteus verdolaga.Toxicon. 2017. 129.:p. 81-88Theraphosidae spider venoms are well known for possess a complex mixture of protein and non-protein compounds in their venom. The objective of this study was to report and identify different proteins translated from the venom gland DNA information of the recently described Theraphosidae spider Pamphobeteus verdolaga. Using a venom gland transcriptomic analysis, we reported a set of the first complete sequences of seven different proteins of the recenlty described Theraphosidae spider P. verdolaga. Protein analysis indicates the presence of different proteins on the venom composition of this new spider, some of them uncommon in the Theraphosidae family. MS/MS analysis of P. verdolaga showed different fragments matching sphingomyelinases (sicaritoxin), barytoxins, hexatoxins, latroinsectotoxins, and linear (zadotoxins) peptides. Only four of the MS/MS fragments showed 100% sequence similarity with one of the transcribed proteins. Transcriptomic analysis showed the presence of different groups of proteins like phospholipases, hyaluronidases, inhibitory cysteine knots (ICK) peptides among others. The three database of protein domains used in this study (Pfam, SMART and CDD) showed congruency in the search of unique conserved protein domain for only four of the translated proteins. Those proteins matched with EF-hand proteins, cysteine rich secretory proteins, jingzhaotoxins, theraphotoxins and hexatoxins, from different Mygalomorphae spiders belonging to the families Theraphosidae, Barychelidae and Hexathelidae. None of the analyzed sequences showed a complete 100% similarity. © 2017 Elsevier Ltdleidy.vargasmu@campusucc.edu.co81-88Elsevier LtdPAMPHOBETEUS VERDOLAGAPROTEOMICTHERAPHOSIDAETRANSCRIPTOMICIdentifying different transcribed proteins in the newly described Theraphosidae Pamphobeteus verdolagaArtículohttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionToxiconinfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Publication20.500.12494/49523oai:repository.ucc.edu.co:20.500.12494/495232024-08-20 16:20:15.003metadata.onlyhttps://repository.ucc.edu.coRepositorio Institucional Universidad Cooperativa de Colombiabdigital@metabiblioteca.com
dc.title.spa.fl_str_mv Identifying different transcribed proteins in the newly described Theraphosidae Pamphobeteus verdolaga
title Identifying different transcribed proteins in the newly described Theraphosidae Pamphobeteus verdolaga
spellingShingle Identifying different transcribed proteins in the newly described Theraphosidae Pamphobeteus verdolaga
PAMPHOBETEUS VERDOLAGA
PROTEOMIC
THERAPHOSIDAE
TRANSCRIPTOMIC
title_short Identifying different transcribed proteins in the newly described Theraphosidae Pamphobeteus verdolaga
title_full Identifying different transcribed proteins in the newly described Theraphosidae Pamphobeteus verdolaga
title_fullStr Identifying different transcribed proteins in the newly described Theraphosidae Pamphobeteus verdolaga
title_full_unstemmed Identifying different transcribed proteins in the newly described Theraphosidae Pamphobeteus verdolaga
title_sort Identifying different transcribed proteins in the newly described Theraphosidae Pamphobeteus verdolaga
dc.creator.fl_str_mv Estrada-Gómez, S.
Vargas Muñoz, Leidy Johana
Saldarriaga Córdoba, M.
Cifuentes, Y.
Perafan, C.
dc.contributor.author.none.fl_str_mv Estrada-Gómez, S.
Vargas Muñoz, Leidy Johana
Saldarriaga Córdoba, M.
Cifuentes, Y.
Perafan, C.
dc.subject.spa.fl_str_mv PAMPHOBETEUS VERDOLAGA
PROTEOMIC
THERAPHOSIDAE
TRANSCRIPTOMIC
topic PAMPHOBETEUS VERDOLAGA
PROTEOMIC
THERAPHOSIDAE
TRANSCRIPTOMIC
description Theraphosidae spider venoms are well known for possess a complex mixture of protein and non-protein compounds in their venom. The objective of this study was to report and identify different proteins translated from the venom gland DNA information of the recently described Theraphosidae spider Pamphobeteus verdolaga. Using a venom gland transcriptomic analysis, we reported a set of the first complete sequences of seven different proteins of the recenlty described Theraphosidae spider P. verdolaga. Protein analysis indicates the presence of different proteins on the venom composition of this new spider, some of them uncommon in the Theraphosidae family. MS/MS analysis of P. verdolaga showed different fragments matching sphingomyelinases (sicaritoxin), barytoxins, hexatoxins, latroinsectotoxins, and linear (zadotoxins) peptides. Only four of the MS/MS fragments showed 100% sequence similarity with one of the transcribed proteins. Transcriptomic analysis showed the presence of different groups of proteins like phospholipases, hyaluronidases, inhibitory cysteine knots (ICK) peptides among others. The three database of protein domains used in this study (Pfam, SMART and CDD) showed congruency in the search of unique conserved protein domain for only four of the translated proteins. Those proteins matched with EF-hand proteins, cysteine rich secretory proteins, jingzhaotoxins, theraphotoxins and hexatoxins, from different Mygalomorphae spiders belonging to the families Theraphosidae, Barychelidae and Hexathelidae. None of the analyzed sequences showed a complete 100% similarity. © 2017 Elsevier Ltd
publishDate 2023
dc.date.issued.none.fl_str_mv 01/04/2017
dc.date.accessioned.none.fl_str_mv 2023-05-24T16:21:29Z
dc.date.available.none.fl_str_mv 2023-05-24T16:21:29Z
dc.type.none.fl_str_mv Artículo
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.coar.none.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.coarversion.none.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.driver.none.fl_str_mv info:eu-repo/semantics/article
dc.type.redcol.none.fl_str_mv http://purl.org/redcol/resource_type/ART
dc.type.version.none.fl_str_mv info:eu-repo/semantics/publishedVersion
format http://purl.org/coar/resource_type/c_6501
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.1016/j.toxicon.2017.02.004
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85013135314&doi=10.1016%2fj.toxicon.2017.02.004&partnerID=40&md5=ff2e53344a833f0e160fc75591c81b02
dc.identifier.issn.spa.fl_str_mv 00410101
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/20.500.12494/49523
dc.identifier.bibliographicCitation.spa.fl_str_mv Estrada-Gómez S.,Vargas Muñoz Leidy johana,Saldarriaga-Córdoba M.,Cifuentes Y.,Perafan C..Identifying different transcribed proteins in the newly described Theraphosidae Pamphobeteus verdolaga.Toxicon. 2017. 129.:p. 81-88
url https://doi.org/10.1016/j.toxicon.2017.02.004
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85013135314&doi=10.1016%2fj.toxicon.2017.02.004&partnerID=40&md5=ff2e53344a833f0e160fc75591c81b02
https://hdl.handle.net/20.500.12494/49523
identifier_str_mv 00410101
Estrada-Gómez S.,Vargas Muñoz Leidy johana,Saldarriaga-Córdoba M.,Cifuentes Y.,Perafan C..Identifying different transcribed proteins in the newly described Theraphosidae Pamphobeteus verdolaga.Toxicon. 2017. 129.:p. 81-88
dc.relation.ispartofjournal.spa.fl_str_mv Toxicon
dc.rights.accessrights.none.fl_str_mv info:eu-repo/semantics/openAccess
dc.rights.coar.none.fl_str_mv http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
rights_invalid_str_mv http://purl.org/coar/access_right/c_abf2
dc.format.extent.spa.fl_str_mv 81-88
dc.publisher.spa.fl_str_mv Elsevier Ltd
institution Universidad Cooperativa de Colombia
repository.name.fl_str_mv Repositorio Institucional Universidad Cooperativa de Colombia
repository.mail.fl_str_mv bdigital@metabiblioteca.com
_version_ 1814247041913585664

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