In vitro anti-HIV-1 activity of the enzymatic extract enriched with laccase produced by the fungi Ganoderma sp. and Lentinus sp.
Artículo Científico
- Autores:
-
Flórez Sampedro, Laura
Zapata Builes, Wildeman
Orozco, Lina P.
Mejía, Amanda I.
Arboleda Echavarría, Carolina
Rugeles López, María Teresa
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2016
- Institución:
- Universidad Cooperativa de Colombia
- Repositorio:
- Repositorio UCC
- Idioma:
- OAI Identifier:
- oai:repository.ucc.edu.co:20.500.12494/1319
- Acceso en línea:
- https://hdl.handle.net/20.500.12494/1319
- Palabra clave:
- Human immunodeficiency virus type 1 (HIV-1)
Ganoderma
Lentinus laccase
Antiviral activity
Natural products
- Rights
- openAccess
- License
- Licencia CC
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| dc.title.spa.fl_str_mv |
In vitro anti-HIV-1 activity of the enzymatic extract enriched with laccase produced by the fungi Ganoderma sp. and Lentinus sp. |
| title |
In vitro anti-HIV-1 activity of the enzymatic extract enriched with laccase produced by the fungi Ganoderma sp. and Lentinus sp. |
| spellingShingle |
In vitro anti-HIV-1 activity of the enzymatic extract enriched with laccase produced by the fungi Ganoderma sp. and Lentinus sp. Human immunodeficiency virus type 1 (HIV-1) Ganoderma Lentinus laccase Antiviral activity Natural products |
| title_short |
In vitro anti-HIV-1 activity of the enzymatic extract enriched with laccase produced by the fungi Ganoderma sp. and Lentinus sp. |
| title_full |
In vitro anti-HIV-1 activity of the enzymatic extract enriched with laccase produced by the fungi Ganoderma sp. and Lentinus sp. |
| title_fullStr |
In vitro anti-HIV-1 activity of the enzymatic extract enriched with laccase produced by the fungi Ganoderma sp. and Lentinus sp. |
| title_full_unstemmed |
In vitro anti-HIV-1 activity of the enzymatic extract enriched with laccase produced by the fungi Ganoderma sp. and Lentinus sp. |
| title_sort |
In vitro anti-HIV-1 activity of the enzymatic extract enriched with laccase produced by the fungi Ganoderma sp. and Lentinus sp. |
| dc.creator.fl_str_mv |
Flórez Sampedro, Laura Zapata Builes, Wildeman Orozco, Lina P. Mejía, Amanda I. Arboleda Echavarría, Carolina Rugeles López, María Teresa |
| dc.contributor.author.none.fl_str_mv |
Flórez Sampedro, Laura Zapata Builes, Wildeman Orozco, Lina P. Mejía, Amanda I. Arboleda Echavarría, Carolina Rugeles López, María Teresa |
| dc.subject.spa.fl_str_mv |
Human immunodeficiency virus type 1 (HIV-1) Ganoderma Lentinus laccase Antiviral activity Natural products |
| topic |
Human immunodeficiency virus type 1 (HIV-1) Ganoderma Lentinus laccase Antiviral activity Natural products |
| description |
Artículo Científico |
| publishDate |
2016 |
| dc.date.issued.none.fl_str_mv |
2016 |
| dc.date.accessioned.none.fl_str_mv |
2017-08-23T20:52:51Z |
| dc.date.available.none.fl_str_mv |
2017-08-23T20:52:51Z |
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Artículo |
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https://hdl.handle.net/20.500.12494/1319 |
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Flórez Sampedro, L.; Zapata, W; Orozco, L. P.; Mejía, A. I.; Arboleda, C.; Rugeles, M. T. (2016). In vitro Anti-HIV-1 activity of the enzymatic extract enriched with laccase produced by the Fungi Ganoderma sp. and Lentinus sp. Vitae. Vitae, Revista de la Facultad de Ciencias Farmacéuticas y Alimentarias. 23(2), pp. 109-118. |
| url |
https://hdl.handle.net/20.500.12494/1319 |
| identifier_str_mv |
Flórez Sampedro, L.; Zapata, W; Orozco, L. P.; Mejía, A. I.; Arboleda, C.; Rugeles, M. T. (2016). In vitro Anti-HIV-1 activity of the enzymatic extract enriched with laccase produced by the Fungi Ganoderma sp. and Lentinus sp. Vitae. Vitae, Revista de la Facultad de Ciencias Farmacéuticas y Alimentarias. 23(2), pp. 109-118. |
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http://aprendeenlinea.udea.edu.co/revistas/index.php/vitae/article/view/23524/20782958 |
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Licencia CC |
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info:eu-repo/semantics/openAccess |
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http://purl.org/coar/access_right/c_abf2 |
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Licencia CC http://purl.org/coar/access_right/c_abf2 |
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openAccess |
| dc.publisher.spa.fl_str_mv |
Universidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Programa de Medicina, Medellín y Envigado, Colombia, 00000 |
| dc.publisher.program.spa.fl_str_mv |
Medicina |
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Medellín |
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Universidad Cooperativa de Colombia |
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Flórez Sampedro, LauraZapata Builes, WildemanOrozco, Lina P.Mejía, Amanda I.Arboleda Echavarría, CarolinaRugeles López, María Teresa2017-08-23T20:52:51Z2017-08-23T20:52:51Z2016https://hdl.handle.net/20.500.12494/1319Flórez Sampedro, L.; Zapata, W; Orozco, L. P.; Mejía, A. I.; Arboleda, C.; Rugeles, M. T. (2016). In vitro Anti-HIV-1 activity of the enzymatic extract enriched with laccase produced by the Fungi Ganoderma sp. and Lentinus sp. Vitae. Vitae, Revista de la Facultad de Ciencias Farmacéuticas y Alimentarias. 23(2), pp. 109-118.Artículo CientíficoBackground: Natural compounds are a good source for the development of antiretroviral drugs with low cytotoxicity. The laccase enzyme, produced by fungi of the genera Ganoderma sp. and Lentinus sp., inhibits the reverse transcriptase (RT) of the human immunodeficiency virus 1 (HIV-1), in cell-free models in vitro. Objetives: In this study we evaluated the anti-HIV-1 activity of the enzymatic extracts (EE) enriched with laccase, produced by two native species of fungi of the same genera in an in vitro cell culture model. Methods: The inhibition of viral replication was performed using the U373-MAGI cell line infected with recombinant viruses in the presence/absence of the EE and 48 hpi, the percentage of infected cells was evaluated by flow cytometry for green fluorescent protein –GFP– and ELISA for p24. The inhibition of the RT was determined by quantification of early and late products of reverse transcription using quantitative PCR. Results: The EEs from Ganoderma sp. and Lentinus sp. inhibited the replication of HIV-1 between 80 and 90% and decreased the production of early and late transcripts between 55,5%-91,3% and 82,1%-93,6% respectively. The EE from Lentinus sp. had the best selectivity index (SI: 8.3). Conclusions: These results suggest the potential anti-HIV-1 activity of the EE for the exploration of an alternative therapy against HIV-1 infection.Abstract. -- Materials and methods. -- Results. -- Discussion. -- Reference.wildeman.zapatab@campusucc.edu.coUniversidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Programa de Medicina, Medellín y Envigado, Colombia, 00000MedicinaMedellínhttp://aprendeenlinea.udea.edu.co/revistas/index.php/vitae/article/view/23524/20782958Human immunodeficiency virus type 1 (HIV-1)GanodermaLentinus laccaseAntiviral activityNatural productsIn vitro anti-HIV-1 activity of the enzymatic extract enriched with laccase produced by the fungi Ganoderma sp. and Lentinus sp.Artículohttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionLicencia 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