Variabilidad genética del cerdo Congo Santandereano mediante marcadores microsatélite

The Congo pig is a zoogenetic resource characteristic of the Santander Department in Colombia. Its genetic diversity was evaluated using 12 different microsatellites, from 37 blood samples. DNA was extracted using Corpogen 2000 kit®; the microsatellites were amplified using PCR (Polymerase Chain Rea...

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Autores:
Jiménez Á.P.
Albarracin Balaguera, Miguel Antonio
Estupiñan Sanabria, Omar Rodrigo
Tipo de recurso:
Article of journal
Fecha de publicación:
2017
Institución:
Universidad Cooperativa de Colombia
Repositorio:
Repositorio UCC
Idioma:
OAI Identifier:
oai:repository.ucc.edu.co:20.500.12494/42123
Acceso en línea:
https://doi.org/10.21830/19006586.89
https://hdl.handle.net/20.500.12494/42123
Palabra clave:
Colombia
Diversity
Pigs
SSR
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closedAccess
License
http://purl.org/coar/access_right/c_14cb
id COOPER2_ba53c0a008ca57657247fd8e77994d52
oai_identifier_str oai:repository.ucc.edu.co:20.500.12494/42123
network_acronym_str COOPER2
network_name_str Repositorio UCC
repository_id_str
spelling Jiménez Á.P.Albarracin Balaguera, Miguel AntonioEstupiñan Sanabria, Omar Rodrigo2021-12-16T22:16:02Z2021-12-16T22:16:02Z2017https://doi.org/10.21830/19006586.8900040592https://hdl.handle.net/20.500.12494/42123Jiménez ÁP,Albarracín M,Estupiñán S. Variabilidad genética del cerdo Congo Santandereano mediante marcadores microsatélite. Arch. Zootec. 2017. 66. (256):p. 599-602. .The Congo pig is a zoogenetic resource characteristic of the Santander Department in Colombia. Its genetic diversity was evaluated using 12 different microsatellites, from 37 blood samples. DNA was extracted using Corpogen 2000 kit®; the microsatellites were amplified using PCR (Polymerase Chain Reaction) and electrophoresis of vertical chamber for typification. Using software (Genetix®) the observed heterozygosity (Ho) and expected heterozygosity (He), the number of alleles for each locus, the allelic frequency and the FIS statistics were calculated. The polymorphic indices (PIC) were calculated using the microsatellite excel Toolkit. The twelve microsatellites employed were polymorphic, a total of 52 alleles were detected, with a median of 4.3 alleles. The (He) range interval values was 0.42 (minimal markers S009 and SW240) – 0.76 (Maximal marker CGA1). The medium Ho was 35%, the majority of the markers were useful with polymorphism information content (PIC) superior to 0.5. The inbreeding index (FIS 0.5) reveals a high deficit of heterozygotes. This finding strongly suggests a low genetic variability of the Santander Congo pig, suggests a possible trend of increase in consanguinity, possibly related to a reduced population size. © 2017, Universidad de Cordoba,Servicio de Publicaciones. All rights reserved.miguel.albarracin@campusucc.edu.co602-599Universidad de Cordoba,Servicio de PublicacionesColombiaDiversityPigsSSRVariabilidad genética del cerdo Congo Santandereano mediante marcadores microsatéliteArtículohttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionArchivos de Zootecniainfo:eu-repo/semantics/closedAccesshttp://purl.org/coar/access_right/c_14cbPublication20.500.12494/42123oai:repository.ucc.edu.co:20.500.12494/421232024-08-20 16:20:09.05metadata.onlyhttps://repository.ucc.edu.coRepositorio Institucional Universidad Cooperativa de Colombiabdigital@metabiblioteca.com
dc.title.spa.fl_str_mv Variabilidad genética del cerdo Congo Santandereano mediante marcadores microsatélite
title Variabilidad genética del cerdo Congo Santandereano mediante marcadores microsatélite
spellingShingle Variabilidad genética del cerdo Congo Santandereano mediante marcadores microsatélite
Colombia
Diversity
Pigs
SSR
title_short Variabilidad genética del cerdo Congo Santandereano mediante marcadores microsatélite
title_full Variabilidad genética del cerdo Congo Santandereano mediante marcadores microsatélite
title_fullStr Variabilidad genética del cerdo Congo Santandereano mediante marcadores microsatélite
title_full_unstemmed Variabilidad genética del cerdo Congo Santandereano mediante marcadores microsatélite
title_sort Variabilidad genética del cerdo Congo Santandereano mediante marcadores microsatélite
dc.creator.fl_str_mv Jiménez Á.P.
Albarracin Balaguera, Miguel Antonio
Estupiñan Sanabria, Omar Rodrigo
dc.contributor.author.none.fl_str_mv Jiménez Á.P.
Albarracin Balaguera, Miguel Antonio
Estupiñan Sanabria, Omar Rodrigo
dc.subject.spa.fl_str_mv Colombia
Diversity
Pigs
SSR
topic Colombia
Diversity
Pigs
SSR
description The Congo pig is a zoogenetic resource characteristic of the Santander Department in Colombia. Its genetic diversity was evaluated using 12 different microsatellites, from 37 blood samples. DNA was extracted using Corpogen 2000 kit®; the microsatellites were amplified using PCR (Polymerase Chain Reaction) and electrophoresis of vertical chamber for typification. Using software (Genetix®) the observed heterozygosity (Ho) and expected heterozygosity (He), the number of alleles for each locus, the allelic frequency and the FIS statistics were calculated. The polymorphic indices (PIC) were calculated using the microsatellite excel Toolkit. The twelve microsatellites employed were polymorphic, a total of 52 alleles were detected, with a median of 4.3 alleles. The (He) range interval values was 0.42 (minimal markers S009 and SW240) – 0.76 (Maximal marker CGA1). The medium Ho was 35%, the majority of the markers were useful with polymorphism information content (PIC) superior to 0.5. The inbreeding index (FIS 0.5) reveals a high deficit of heterozygotes. This finding strongly suggests a low genetic variability of the Santander Congo pig, suggests a possible trend of increase in consanguinity, possibly related to a reduced population size. © 2017, Universidad de Cordoba,Servicio de Publicaciones. All rights reserved.
publishDate 2017
dc.date.issued.none.fl_str_mv 2017
dc.date.accessioned.none.fl_str_mv 2021-12-16T22:16:02Z
dc.date.available.none.fl_str_mv 2021-12-16T22:16:02Z
dc.type.none.fl_str_mv Artículo
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.coar.none.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.coarversion.none.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.driver.none.fl_str_mv info:eu-repo/semantics/article
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dc.type.version.none.fl_str_mv info:eu-repo/semantics/publishedVersion
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status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.21830/19006586.89
dc.identifier.issn.spa.fl_str_mv 00040592
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/20.500.12494/42123
dc.identifier.bibliographicCitation.spa.fl_str_mv Jiménez ÁP,Albarracín M,Estupiñán S. Variabilidad genética del cerdo Congo Santandereano mediante marcadores microsatélite. Arch. Zootec. 2017. 66. (256):p. 599-602. .
url https://doi.org/10.21830/19006586.89
https://hdl.handle.net/20.500.12494/42123
identifier_str_mv 00040592
Jiménez ÁP,Albarracín M,Estupiñán S. Variabilidad genética del cerdo Congo Santandereano mediante marcadores microsatélite. Arch. Zootec. 2017. 66. (256):p. 599-602. .
dc.relation.ispartofjournal.spa.fl_str_mv Archivos de Zootecnia
dc.rights.accessrights.none.fl_str_mv info:eu-repo/semantics/closedAccess
dc.rights.coar.none.fl_str_mv http://purl.org/coar/access_right/c_14cb
eu_rights_str_mv closedAccess
rights_invalid_str_mv http://purl.org/coar/access_right/c_14cb
dc.format.extent.spa.fl_str_mv 602-599
dc.publisher.spa.fl_str_mv Universidad de Cordoba,Servicio de Publicaciones
institution Universidad Cooperativa de Colombia
repository.name.fl_str_mv Repositorio Institucional Universidad Cooperativa de Colombia
repository.mail.fl_str_mv bdigital@metabiblioteca.com
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