HIV-1 induces the first signal to activate the NLRP3 inflammasome in monocyte-derived macrophages

Background/Aims: Inflammasomes are multimolecular complexes that regulate caspase-1. They act as sensors for endogenous and exogenous signals, and mediate the processing of pro-IL-1ß into its secreted, biologically active form. The NLRP3 inflammasome and IL-1ß are particularly interesting because they are required for efficient control of viral infections. Indeed, HIV-1 induces expression of NLRP3 and IL-1ß in healthy controls, but not in HIV-1-infected patients. Here we evaluate whether HIV-1 can induce activation of the NLRP3 inflammasome. Methods: Human primary monocyte-derived macrophages were infected with HIV-1 in the absence or presence of classical NLRP3 inflammasome activators, and IL-1ß release was assessed by ELISA. Results: HIV-1 initiates the priming signal for NLRP3 inflammasome activation through the NF-?B-associated pathway in human primary monocyte-derived macrophages. Furthermore, priming of NLRP3 activation in response to HIV-1 was independent of the viral envelope, since similar results were observed with HIV-1 and pseudotyped HIV-1 lacking the env gene. Conclusion: Our findings suggest that HIV-1 infection promotes IL-1ß secretion by inducing the first signal for NLRP3 inflammasome activation, a phenomenon that may contribute to AIDS progression. Copyright © 2013 S. Karger AG, Basel.

• Autores: Hernández López, Juan Carlos; Latz, E.; Urcuqui Inchima, S.
• Tipo de recurso: Article of journal
• Fecha de publicación: 2023
• Institución: Universidad Cooperativa de Colombia
• Repositorio: Repositorio UCC
• OAI Identifier: oai:repository.ucc.edu.co:20.500.12494/49709
• Acceso en línea: https://doi.org/10.1159/000353902; https://www.scopus.com/inward/record.uri?eid=2-s2.0-84889820913&doi=10.1159%2f000353902&partnerID=40&md5=c3cb5bba2776cb898287c152a9a55a59; https://hdl.handle.net/20.500.12494/49709
• Palabra clave: ARTICLE; CARRIER PROTEINS; CELLS, CULTURED; CONTROLLED STUDY; CRYOPYRIN; CYTOKINE RELEASE; ENVELOPE GENE; ENZYME LINKED IMMUNOSORBENT ASSAY; ENZYME-LINKED IMMUNOSORBENT ASSAY; HIV-1; HUMAN; HUMAN CELL; HUMAN IMMUNODEFICIENCY VIRUS 1; HUMAN IMMUNODEFICIENCY VIRUS 1 INFECTION; HUMANS; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; INFLAMMASOME; INFLAMMASOMES; INTERLEUKIN 1BETA; INTERLEUKIN-1BETA; MACROPHAGE; MACROPHAGES; MONOCYTE; NONHUMAN; PRIORITY JOURNAL; PROTEIN INDUCTION; SIGNAL TRANSDUCTION; VIRUS ENVELOPE
• Rights: openAccess
• License: http://purl.org/coar/access_right/c_abf2