Profiling analysis of circulating microRNA in peripheral blood of patients with class IV lupus nephritis

Renal involvement in Systemic Lupus Erythematous (SLE) patients is one of the leading causes of morbidity and a significant contributor to mortality. It’s estimated that nearly 50% of SLE individuals develop kidney disease in the first year of the diagnosis. Class IV lupus nephritis (LN-IV) is the c...

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Autores:
Navarro Quiroz, Elkin Antonio
Pacheco Lugo, Lisandro
Tipo de recurso:
Article of journal
Fecha de publicación:
2017
Institución:
Universidad Cooperativa de Colombia
Repositorio:
Repositorio UCC
Idioma:
OAI Identifier:
oai:repository.ucc.edu.co:20.500.12494/1827
Acceso en línea:
https://hdl.handle.net/20.500.12494/1827
Palabra clave:
Circulating microRNA
Rights
openAccess
License
Licencia CC
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dc.title.spa.fl_str_mv Profiling analysis of circulating microRNA in peripheral blood of patients with class IV lupus nephritis
title Profiling analysis of circulating microRNA in peripheral blood of patients with class IV lupus nephritis
spellingShingle Profiling analysis of circulating microRNA in peripheral blood of patients with class IV lupus nephritis
Circulating microRNA
title_short Profiling analysis of circulating microRNA in peripheral blood of patients with class IV lupus nephritis
title_full Profiling analysis of circulating microRNA in peripheral blood of patients with class IV lupus nephritis
title_fullStr Profiling analysis of circulating microRNA in peripheral blood of patients with class IV lupus nephritis
title_full_unstemmed Profiling analysis of circulating microRNA in peripheral blood of patients with class IV lupus nephritis
title_sort Profiling analysis of circulating microRNA in peripheral blood of patients with class IV lupus nephritis
dc.creator.fl_str_mv Navarro Quiroz, Elkin Antonio
Pacheco Lugo, Lisandro
dc.contributor.author.none.fl_str_mv Navarro Quiroz, Elkin Antonio
Pacheco Lugo, Lisandro
dc.subject.spa.fl_str_mv Circulating microRNA
topic Circulating microRNA
description Renal involvement in Systemic Lupus Erythematous (SLE) patients is one of the leading causes of morbidity and a significant contributor to mortality. It’s estimated that nearly 50% of SLE individuals develop kidney disease in the first year of the diagnosis. Class IV lupus nephritis (LN-IV) is the class of lupus nephritis most common in Colombian patients with SLE. Altered miRNAs expression levels have been reported in human autoimmune diseases including lupus. Variations in the expression pattern of peripheral blood circulating miRNAs specific for this class of lupus nephritis could be correlated with the pathophysiological status of this group of individuals. The aim of this study was to evaluate the relative abundance of circulating microRNAs in peripheral blood from Colombian patients with LN-IV. Circulating miRNAs in plasma of patients with diagnosis of LN-IV were compared with individuals without renal involvement (LNN group) and healthy individuals (CTL group). Total RNA was extracted from 10 ml of venous blood and subsequently sequenced using Illumina. The sequences were processed and these were analyzed using miRBase and Ensembl databases. Differential gene expression analysis was carried out with edgeR and functional analysis were done with DIANA-miRPath. Analysis was carried out using as variables of selection fold change ( 2 o -2) and false discovery rate (0.05). We identified 24 circulating microRNAs with differential abundance between LN-IV and CTL groups, fourteen of these microRNAs are described for the first time to lupus nephritis (hsa-miR-589-3p, hsa-miR-1260b, hsa-miR-4511, hsa-miR485-5p, hsa-miR-584-5p, hsa-miR-543, hsa-miR-153-3p, hsa-miR-6087, hsa-miR-3942-5p, hsa-miR-7977, hsa-miR-323b-3p, hsa-miR-4732-3p and hsa-miR-6741-3p). These changes in the abundance of miRNAs could be interpreted as alterations in the miRNAs-mRNA regulatory network in the pathogenesis of LN, preceding the clinical onset of the disease. The findings thus contribute to understanding the disease process and are likely to pave the way towards identifying disease biomarkers for early diagnosis of LN.
publishDate 2017
dc.date.issued.none.fl_str_mv 2017-11-14
dc.date.accessioned.none.fl_str_mv 2018-03-02T17:38:50Z
dc.date.available.none.fl_str_mv 2018-03-02T17:38:50Z
dc.type.none.fl_str_mv Artículo
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dc.identifier.bibliographicCitation.spa.fl_str_mv Navarro-Quiroz, E.; Pacheco-Lugo, L.; Navarro-Quiroz R, Lorenzi, H., España-Puccini, P., Díaz-Olmos, Y.; et al. (2017) Profiling analysis of circulating microRNA in peripheral blood of patients with class IV lupus nephritis. PLoS ONE, 12 (11): e0187973. https://doi.org/10.1371/journal. pone.0187973
url https://hdl.handle.net/20.500.12494/1827
identifier_str_mv Navarro-Quiroz, E.; Pacheco-Lugo, L.; Navarro-Quiroz R, Lorenzi, H., España-Puccini, P., Díaz-Olmos, Y.; et al. (2017) Profiling analysis of circulating microRNA in peripheral blood of patients with class IV lupus nephritis. PLoS ONE, 12 (11): e0187973. https://doi.org/10.1371/journal. pone.0187973
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dc.rights.cc.none.fl_str_mv Licencia CC
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dc.publisher.spa.fl_str_mv Universidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Medicina, Santa Marta
dc.publisher.program.spa.fl_str_mv Medicina
dc.publisher.place.spa.fl_str_mv Santa Marta
institution Universidad Cooperativa de Colombia
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spelling Navarro Quiroz, Elkin AntonioPacheco Lugo, Lisandro2018-03-02T17:38:50Z2018-03-02T17:38:50Z2017-11-14https://hdl.handle.net/20.500.12494/1827Navarro-Quiroz, E.; Pacheco-Lugo, L.; Navarro-Quiroz R, Lorenzi, H., España-Puccini, P., Díaz-Olmos, Y.; et al. (2017) Profiling analysis of circulating microRNA in peripheral blood of patients with class IV lupus nephritis. PLoS ONE, 12 (11): e0187973. https://doi.org/10.1371/journal. pone.0187973Renal involvement in Systemic Lupus Erythematous (SLE) patients is one of the leading causes of morbidity and a significant contributor to mortality. It’s estimated that nearly 50% of SLE individuals develop kidney disease in the first year of the diagnosis. Class IV lupus nephritis (LN-IV) is the class of lupus nephritis most common in Colombian patients with SLE. Altered miRNAs expression levels have been reported in human autoimmune diseases including lupus. Variations in the expression pattern of peripheral blood circulating miRNAs specific for this class of lupus nephritis could be correlated with the pathophysiological status of this group of individuals. The aim of this study was to evaluate the relative abundance of circulating microRNAs in peripheral blood from Colombian patients with LN-IV. Circulating miRNAs in plasma of patients with diagnosis of LN-IV were compared with individuals without renal involvement (LNN group) and healthy individuals (CTL group). Total RNA was extracted from 10 ml of venous blood and subsequently sequenced using Illumina. The sequences were processed and these were analyzed using miRBase and Ensembl databases. Differential gene expression analysis was carried out with edgeR and functional analysis were done with DIANA-miRPath. Analysis was carried out using as variables of selection fold change ( 2 o -2) and false discovery rate (0.05). We identified 24 circulating microRNAs with differential abundance between LN-IV and CTL groups, fourteen of these microRNAs are described for the first time to lupus nephritis (hsa-miR-589-3p, hsa-miR-1260b, hsa-miR-4511, hsa-miR485-5p, hsa-miR-584-5p, hsa-miR-543, hsa-miR-153-3p, hsa-miR-6087, hsa-miR-3942-5p, hsa-miR-7977, hsa-miR-323b-3p, hsa-miR-4732-3p and hsa-miR-6741-3p). These changes in the abundance of miRNAs could be interpreted as alterations in the miRNAs-mRNA regulatory network in the pathogenesis of LN, preceding the clinical onset of the disease. The findings thus contribute to understanding the disease process and are likely to pave the way towards identifying disease biomarkers for early diagnosis of LN.Abstract. -- Introduction. --enavarro26@unisimonbolivar.edu.coUniversidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Medicina, Santa MartaMedicinaSanta Martahttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0187973Circulating microRNAProfiling analysis of circulating microRNA in peripheral blood of patients with class IV lupus nephritisArtículohttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionLicencia 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