Efecto del estatus ovárico sobre la producción in vitro de embriones ovinos
El objetivo de este estudio fue evaluar el efecto del estatus ovárico sobre la producción in vitro de embriones ovinos. Fueron colectados pares de ovarios provenientes de una planta de beneficio ubicada en la ciudad de Bucaramanga, y transportados para ser procesados en el laboratorio de Biotecnolog...
- Autores:
-
Remolina Castrillón, Wendy
- Tipo de recurso:
- Trabajo de grado de pregrado
- Fecha de publicación:
- 2022
- Institución:
- Universidad Cooperativa de Colombia
- Repositorio:
- Repositorio UCC
- Idioma:
- OAI Identifier:
- oai:repository.ucc.edu.co:20.500.12494/46282
- Acceso en línea:
- https://hdl.handle.net/20.500.12494/46282
- Palabra clave:
- Blastocistos
Cuerpo lúteo
Ovinos
Fecundación in vitro
TG 2022 MVZ 46282
Blastocysts
Corpus luteum
Sheep
In vitro fertilization
- Rights
- openAccess
- License
- Atribución – No comercial – Sin Derivar
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Efecto del estatus ovárico sobre la producción in vitro de embriones ovinos |
| title |
Efecto del estatus ovárico sobre la producción in vitro de embriones ovinos |
| spellingShingle |
Efecto del estatus ovárico sobre la producción in vitro de embriones ovinos Blastocistos Cuerpo lúteo Ovinos Fecundación in vitro TG 2022 MVZ 46282 Blastocysts Corpus luteum Sheep In vitro fertilization |
| title_short |
Efecto del estatus ovárico sobre la producción in vitro de embriones ovinos |
| title_full |
Efecto del estatus ovárico sobre la producción in vitro de embriones ovinos |
| title_fullStr |
Efecto del estatus ovárico sobre la producción in vitro de embriones ovinos |
| title_full_unstemmed |
Efecto del estatus ovárico sobre la producción in vitro de embriones ovinos |
| title_sort |
Efecto del estatus ovárico sobre la producción in vitro de embriones ovinos |
| dc.creator.fl_str_mv |
Remolina Castrillón, Wendy |
| dc.contributor.advisor.none.fl_str_mv |
Moreno Jerez, Edgar Ricardo |
| dc.contributor.author.none.fl_str_mv |
Remolina Castrillón, Wendy |
| dc.subject.spa.fl_str_mv |
Blastocistos Cuerpo lúteo Ovinos Fecundación in vitro |
| topic |
Blastocistos Cuerpo lúteo Ovinos Fecundación in vitro TG 2022 MVZ 46282 Blastocysts Corpus luteum Sheep In vitro fertilization |
| dc.subject.classification.spa.fl_str_mv |
TG 2022 MVZ 46282 |
| dc.subject.other.spa.fl_str_mv |
Blastocysts Corpus luteum Sheep In vitro fertilization |
| description |
El objetivo de este estudio fue evaluar el efecto del estatus ovárico sobre la producción in vitro de embriones ovinos. Fueron colectados pares de ovarios provenientes de una planta de beneficio ubicada en la ciudad de Bucaramanga, y transportados para ser procesados en el laboratorio de Biotecnología de la reproducción animal LBRA de la Universidad Cooperativa de Colombia UCC. Inicialmente, fueron clasificados de acuerdo con la presencia de cuerpo lúteo (CL) en dos grupos de la siguiente manera: Monovulatorios+ (M+): con presencia de un cuerpo lúteo ; Poliovulatorios+ (P+): más de un cuerpo lúteo en el par de ovarios; y a partir de esta clasificación, se derivaron otros tres grupos, de la siguiente manera: Monovulatorios- (M-): sin cuerpo lúteo, pero proveniente de un animal con un cuerpo lúteo en el ovario contralateral; Poliovulatorios- (P-): sin cuerpo lúteo, pero proveniente de un animal con más de un cuerpo lúteo en el ovario contralateral; Sin cuerpo lúteo (NCL): ovarios de hembras en anestro. A partir de los 5 grupos experimentales propuestos, los ovocitos fueron recuperados, contados y clasificados, de acuerdo con su morfología en cuatro grados de calidad, y únicamente los calificados como grado I y grado II de cada grupo fueron sometidos a maduración in vitro (MIV). La MIV de los CCOs se realizó en incubadora durante 22-24 horas, en gotas de 100 µL de medio, Una vez transcurrido el tiempo de maduración, los ovocitos fueron sometidos a fertilización in vitro (FIV) mediante co -incubación durante 18-22 horas con 4 millones de espermatozoides/mL congelados/descongelados. Finalmente, los posibles embriones fueron cultivados in vitro (CIV) durante 7 días en incubadora a 38,5°C, humedad saturada, y una mezcla de gases compuesta por 90% nitrógeno, 5% CO2, y 5% O2. Fueron evaluadas las tasas de clivaje y la producción de blastocistos, a las 48 h y a los 6 y 7 días del CIV, respectivamente. |
| publishDate |
2022 |
| dc.date.accessioned.none.fl_str_mv |
2022-09-02T15:27:10Z |
| dc.date.available.none.fl_str_mv |
2022-09-02T15:27:10Z |
| dc.date.issued.none.fl_str_mv |
2022-08-31 |
| dc.type.none.fl_str_mv |
Trabajo de grado - Pregrado |
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http://purl.org/coar/resource_type/c_7a1f |
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info:eu-repo/semantics/bachelorThesis |
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info:eu-repo/semantics/acceptedVersion |
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http://purl.org/coar/resource_type/c_7a1f |
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acceptedVersion |
| dc.identifier.uri.none.fl_str_mv |
https://hdl.handle.net/20.500.12494/46282 |
| dc.identifier.bibliographicCitation.spa.fl_str_mv |
Remolina Castrillón, W. (2022). Efecto del estatus ovárico sobre la producción in vitro de embriones ovinos. [Tesis de pregrado, Universidad Cooperativa de Colombia]. Repositorio Institucional UCC. https://repository.ucc.edu.co/handle/20.500.12494/46282 |
| url |
https://hdl.handle.net/20.500.12494/46282 |
| identifier_str_mv |
Remolina Castrillón, W. (2022). Efecto del estatus ovárico sobre la producción in vitro de embriones ovinos. [Tesis de pregrado, Universidad Cooperativa de Colombia]. Repositorio Institucional UCC. https://repository.ucc.edu.co/handle/20.500.12494/46282 |
| dc.relation.references.spa.fl_str_mv |
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Oxygen tension during in vitro oocyte maturation and fertilization affects embryo quality in sheep and deer. Anim Reprod Ciencia. 2020. Reza-Ebrahimi M, Mara L, Parham A, Dattena M. Reduced effect of mineral oil toxicity using four-well culture dish in sheep embryo production. Small Ruminant Research. 2020. Leifried L, First N. 1979. Characterization of bovine follicular oocytes and their ability to mature in vitro. |
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Universidad Coopertativa de Colombia, Facultad de Ciencias de la Salud, Medicina Veterinaria y Zootecnia, Bucaramanga |
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Moreno Jerez, Edgar RicardoRemolina Castrillón, Wendy2022-09-02T15:27:10Z2022-09-02T15:27:10Z2022-08-31https://hdl.handle.net/20.500.12494/46282Remolina Castrillón, W. (2022). Efecto del estatus ovárico sobre la producción in vitro de embriones ovinos. [Tesis de pregrado, Universidad Cooperativa de Colombia]. Repositorio Institucional UCC. https://repository.ucc.edu.co/handle/20.500.12494/46282El objetivo de este estudio fue evaluar el efecto del estatus ovárico sobre la producción in vitro de embriones ovinos. Fueron colectados pares de ovarios provenientes de una planta de beneficio ubicada en la ciudad de Bucaramanga, y transportados para ser procesados en el laboratorio de Biotecnología de la reproducción animal LBRA de la Universidad Cooperativa de Colombia UCC. Inicialmente, fueron clasificados de acuerdo con la presencia de cuerpo lúteo (CL) en dos grupos de la siguiente manera: Monovulatorios+ (M+): con presencia de un cuerpo lúteo ; Poliovulatorios+ (P+): más de un cuerpo lúteo en el par de ovarios; y a partir de esta clasificación, se derivaron otros tres grupos, de la siguiente manera: Monovulatorios- (M-): sin cuerpo lúteo, pero proveniente de un animal con un cuerpo lúteo en el ovario contralateral; Poliovulatorios- (P-): sin cuerpo lúteo, pero proveniente de un animal con más de un cuerpo lúteo en el ovario contralateral; Sin cuerpo lúteo (NCL): ovarios de hembras en anestro. A partir de los 5 grupos experimentales propuestos, los ovocitos fueron recuperados, contados y clasificados, de acuerdo con su morfología en cuatro grados de calidad, y únicamente los calificados como grado I y grado II de cada grupo fueron sometidos a maduración in vitro (MIV). La MIV de los CCOs se realizó en incubadora durante 22-24 horas, en gotas de 100 µL de medio, Una vez transcurrido el tiempo de maduración, los ovocitos fueron sometidos a fertilización in vitro (FIV) mediante co -incubación durante 18-22 horas con 4 millones de espermatozoides/mL congelados/descongelados. Finalmente, los posibles embriones fueron cultivados in vitro (CIV) durante 7 días en incubadora a 38,5°C, humedad saturada, y una mezcla de gases compuesta por 90% nitrógeno, 5% CO2, y 5% O2. Fueron evaluadas las tasas de clivaje y la producción de blastocistos, a las 48 h y a los 6 y 7 días del CIV, respectivamente.The objective of this study was to evaluate the effect of ovarian status on the in vitro production of sheep embryos. Pairs of ovaries were collected from a processing plant located in the city of Bucaramanga and transported to be processed in the LBRA Animal Reproduction Biotechnology Laboratory of the Universidad Cooperative de Colombia UCC. Initially, they were classified according to the presence of the corpus luteum (CL) into two groups as follows: Monovulatory + (M +): with the presence of a corpus luteum; Poliovulatory + (P +): more than one corpus luteum in the pair of ovaries; and from this classification, three other groups were derived, as follows: Monovulatory- (M-): without corpus luteum, but from an animal with a corpus luteum in the contralateral ovary; Poliovulatory- (P-): without corpus luteum, but from an animal with more than one corpus luteum in the contralateral ovary; Without corpus luteum (NCL): female ovaries in anestrus. From the 5 proposed experimental groups, the oocytes were recovered, counted and classified, according to their morphology into four quality grades, and only those classified as grade I and grade II of each group were subjected to in vitro maturation (IVM ). IVM of the CCOs was performed in an incubator for 22-24 hours, in drops of 100 µL of medium. Once the maturation time had elapsed, the oocytes were subjected to in vitro fertilization (IVF) by co-incubation for 18-22 hours. hours with 4 million frozen / thawed sperm / mL. Finally, the possible embryos were cultured in vitro (IVC) for 7 days in an incubator at 38.5 ° C, saturated humidity, and a gas mixture composed of 90% nitrogen, 5% CO2, and 5% O2. Cleavage rates and blastocyst production were evaluated at 48 h and at 6 and 7 days after VSD, respectively.Resumen. -- Introducción. -- 1. Planteamiento del problema. -- 2. Justificación. -- 3. Objetivos. -- 3.1. Objetivo general. -- 3.2. Objetivos específicos. -- 4. Marco teórico. -- 4.1. Etapas de la Producción in vitro de Embriones. -- 4.1.1. Maduración Ovocitaria. -- 4.1.2. Preparación Espermática. -- 4.1.3. Fertilización in vitro. -- 4.1.4. Cultivo in vitro. -- 4.2. Competencia ovocitaria. -- 4.2.1. Factores que afectan la competencia ovocitaria. -- 5. Materiales y métodos. -- 5.1. Colecta de ovarios. -- 5.2. Grupos experimentales. -- 5.3. Recuperación y clasificación de los CCOs. -- 5.4. Maduración in vitro de los ovocitos. -- 5.5. Fertilización in vitro. -- 5.6. Cultivo in vitro. -- 5.7. Evaluación de la tasa de clivaje y producción de blastocistos. -- 6. Resultados y Discusión. -- 7. Conclusiones. -- 8. Recomendaciones. -- 9. Referencias Bibliográficas.wendy.remolinac@campusucc.edu.co69 p.Universidad Coopertativa de Colombia, Facultad de Ciencias de la Salud, Medicina Veterinaria y Zootecnia, BucaramangaMedicina veterinaria y zootecniaBucaramangaBlastocistosCuerpo lúteoOvinosFecundación in vitroTG 2022 MVZ 46282BlastocystsCorpus luteumSheepIn vitro fertilizationEfecto del estatus ovárico sobre la producción in vitro de embriones ovinosTrabajo de grado - Pregradohttp://purl.org/coar/resource_type/c_7a1finfo:eu-repo/semantics/bachelorThesisinfo:eu-repo/semantics/acceptedVersionAtribución – No comercial – Sin Derivarinfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Agronews Castilla y León. "Beneficios y propiedades de la carne de cordero".FAOSTAT-DATA. Live Animals.Zhu J, Moawad AR, Wang CY, Li HF, Ren JY, Dai YF. Advances in in vitro production of sheep embryos. International Journal of Veterinary Science and Medicine. 2018; p. 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