HIV-1 induces the first signal to activate the NLRP3 inflammasome in monocyte-derived macrophages

Background/Aims: Inflammasomes are multimolecular complexes that regulate caspase-1. They act as sensors for endogenous and exogenous signals, and mediate the processing of pro-IL-1ß into its secreted, biologically active form. The NLRP3 inflammasome and IL-1ß are particularly interesting because th...

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Autores:
Hernández López, Juan Carlos
Latz E.
Urcuqui-Inchima S.
Tipo de recurso:
Article of journal
Fecha de publicación:
2013
Institución:
Universidad Cooperativa de Colombia
Repositorio:
Repositorio UCC
Idioma:
OAI Identifier:
oai:repository.ucc.edu.co:20.500.12494/41805
Acceso en línea:
https://doi.org/10.16925/di.v18i23.1292
https://hdl.handle.net/20.500.12494/41805
Palabra clave:
cryopyrin
immunoglobulin enhancer binding protein
inflammasome
interleukin 1beta
immunoglobulin enhancer binding protein
interleukin 1beta
article
controlled study
cytokine release
enzyme linked immunosorbent assay
human
human cell
Human immunodeficiency virus 1 infection
macrophage
priority journal
protein induction
signal transduction
virus envelope
Article
envelope gene
Human immunodeficiency virus 1
Human immunodeficiency virus 1 infection
macrophage
monocyte
nonhuman
Carrier Proteins
Cells
Cultured
Enzyme-Linked Immunosorbent Assay
HIV-1
Humans
Inflammasomes
Interleukin-1beta
Macrophages
Human immunodeficiency virus 1
Rights
closedAccess
License
http://purl.org/coar/access_right/c_14cb
id COOPER2_b0f5102eb135ed009b2d42e037029bd6
oai_identifier_str oai:repository.ucc.edu.co:20.500.12494/41805
network_acronym_str COOPER2
network_name_str Repositorio UCC
repository_id_str
spelling Hernández López, Juan Carlos Latz E.Urcuqui-Inchima S.2021-12-16T22:15:48Z2021-12-16T22:15:48Z2013https://doi.org/10.16925/di.v18i23.129203005526https://hdl.handle.net/20.500.12494/41805Hernandez JC,Latz E,Urcuqui S. HIV-1 induces the first signal to activate the NLRP3 inflammasome in monocyte-derived macrophages. Intervirology. 2013. 57. (1):p. 36-42. .Background/Aims: Inflammasomes are multimolecular complexes that regulate caspase-1. They act as sensors for endogenous and exogenous signals, and mediate the processing of pro-IL-1ß into its secreted, biologically active form. The NLRP3 inflammasome and IL-1ß are particularly interesting because they are required for efficient control of viral infections. Indeed, HIV-1 induces expression of NLRP3 and IL-1ß in healthy controls, but not in HIV-1-infected patients. Here we evaluate whether HIV-1 can induce activation of the NLRP3 inflammasome. Methods: Human primary monocyte-derived macrophages were infected with HIV-1 in the absence or presence of classical NLRP3 inflammasome activators, and IL-1ß release was assessed by ELISA. Results: HIV-1 initiates the priming signal for NLRP3 inflammasome activation through the NF-?B-associated pathway in human primary monocyte-derived macrophages. Furthermore, priming of NLRP3 activation in response to HIV-1 was independent of the viral envelope, since similar results were observed with HIV-1 and pseudotyped HIV-1 lacking the env gene. Conclusion: Our findings suggest that HIV-1 infection promotes IL-1ß secretion by inducing the first signal for NLRP3 inflammasome activation, a phenomenon that may contribute to AIDS progression. Copyright © 2013 S. Karger AG, Basel.0000-0002-9200-5698juanc.hernandezl@campusucc.edu.co42-36S. Karger AGcryopyrinimmunoglobulin enhancer binding proteininflammasomeinterleukin 1betaimmunoglobulin enhancer binding proteininterleukin 1betaarticlecontrolled studycytokine releaseenzyme linked immunosorbent assayhumanhuman cellHuman immunodeficiency virus 1 infectionmacrophagepriority journalprotein inductionsignal transductionvirus envelopeArticleenvelope geneHuman immunodeficiency virus 1Human immunodeficiency virus 1 infectionmacrophagemonocytenonhumanCarrier ProteinsCellsCulturedEnzyme-Linked Immunosorbent AssayHIV-1HumansInflammasomesInterleukin-1betaMacrophagesHuman immunodeficiency virus 1HIV-1 induces the first signal to activate the NLRP3 inflammasome in monocyte-derived macrophagesArtículohttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionIntervirologyinfo:eu-repo/semantics/closedAccesshttp://purl.org/coar/access_right/c_14cbPublication20.500.12494/41805oai:repository.ucc.edu.co:20.500.12494/418052024-08-20 16:21:13.441metadata.onlyhttps://repository.ucc.edu.coRepositorio Institucional Universidad Cooperativa de Colombiabdigital@metabiblioteca.com
dc.title.spa.fl_str_mv HIV-1 induces the first signal to activate the NLRP3 inflammasome in monocyte-derived macrophages
title HIV-1 induces the first signal to activate the NLRP3 inflammasome in monocyte-derived macrophages
spellingShingle HIV-1 induces the first signal to activate the NLRP3 inflammasome in monocyte-derived macrophages
cryopyrin
immunoglobulin enhancer binding protein
inflammasome
interleukin 1beta
immunoglobulin enhancer binding protein
interleukin 1beta
article
controlled study
cytokine release
enzyme linked immunosorbent assay
human
human cell
Human immunodeficiency virus 1 infection
macrophage
priority journal
protein induction
signal transduction
virus envelope
Article
envelope gene
Human immunodeficiency virus 1
Human immunodeficiency virus 1 infection
macrophage
monocyte
nonhuman
Carrier Proteins
Cells
Cultured
Enzyme-Linked Immunosorbent Assay
HIV-1
Humans
Inflammasomes
Interleukin-1beta
Macrophages
Human immunodeficiency virus 1
title_short HIV-1 induces the first signal to activate the NLRP3 inflammasome in monocyte-derived macrophages
title_full HIV-1 induces the first signal to activate the NLRP3 inflammasome in monocyte-derived macrophages
title_fullStr HIV-1 induces the first signal to activate the NLRP3 inflammasome in monocyte-derived macrophages
title_full_unstemmed HIV-1 induces the first signal to activate the NLRP3 inflammasome in monocyte-derived macrophages
title_sort HIV-1 induces the first signal to activate the NLRP3 inflammasome in monocyte-derived macrophages
dc.creator.fl_str_mv Hernández López, Juan Carlos
Latz E.
Urcuqui-Inchima S.
dc.contributor.author.none.fl_str_mv Hernández López, Juan Carlos
Latz E.
Urcuqui-Inchima S.
dc.subject.spa.fl_str_mv cryopyrin
immunoglobulin enhancer binding protein
inflammasome
interleukin 1beta
immunoglobulin enhancer binding protein
interleukin 1beta
article
controlled study
cytokine release
enzyme linked immunosorbent assay
human
human cell
Human immunodeficiency virus 1 infection
macrophage
priority journal
protein induction
signal transduction
virus envelope
Article
envelope gene
Human immunodeficiency virus 1
Human immunodeficiency virus 1 infection
macrophage
monocyte
nonhuman
Carrier Proteins
Cells
Cultured
Enzyme-Linked Immunosorbent Assay
HIV-1
Humans
Inflammasomes
Interleukin-1beta
Macrophages
Human immunodeficiency virus 1
topic cryopyrin
immunoglobulin enhancer binding protein
inflammasome
interleukin 1beta
immunoglobulin enhancer binding protein
interleukin 1beta
article
controlled study
cytokine release
enzyme linked immunosorbent assay
human
human cell
Human immunodeficiency virus 1 infection
macrophage
priority journal
protein induction
signal transduction
virus envelope
Article
envelope gene
Human immunodeficiency virus 1
Human immunodeficiency virus 1 infection
macrophage
monocyte
nonhuman
Carrier Proteins
Cells
Cultured
Enzyme-Linked Immunosorbent Assay
HIV-1
Humans
Inflammasomes
Interleukin-1beta
Macrophages
Human immunodeficiency virus 1
description Background/Aims: Inflammasomes are multimolecular complexes that regulate caspase-1. They act as sensors for endogenous and exogenous signals, and mediate the processing of pro-IL-1ß into its secreted, biologically active form. The NLRP3 inflammasome and IL-1ß are particularly interesting because they are required for efficient control of viral infections. Indeed, HIV-1 induces expression of NLRP3 and IL-1ß in healthy controls, but not in HIV-1-infected patients. Here we evaluate whether HIV-1 can induce activation of the NLRP3 inflammasome. Methods: Human primary monocyte-derived macrophages were infected with HIV-1 in the absence or presence of classical NLRP3 inflammasome activators, and IL-1ß release was assessed by ELISA. Results: HIV-1 initiates the priming signal for NLRP3 inflammasome activation through the NF-?B-associated pathway in human primary monocyte-derived macrophages. Furthermore, priming of NLRP3 activation in response to HIV-1 was independent of the viral envelope, since similar results were observed with HIV-1 and pseudotyped HIV-1 lacking the env gene. Conclusion: Our findings suggest that HIV-1 infection promotes IL-1ß secretion by inducing the first signal for NLRP3 inflammasome activation, a phenomenon that may contribute to AIDS progression. Copyright © 2013 S. Karger AG, Basel.
publishDate 2013
dc.date.issued.none.fl_str_mv 2013
dc.date.accessioned.none.fl_str_mv 2021-12-16T22:15:48Z
dc.date.available.none.fl_str_mv 2021-12-16T22:15:48Z
dc.type.none.fl_str_mv Artículo
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.coar.none.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.coarversion.none.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.driver.none.fl_str_mv info:eu-repo/semantics/article
dc.type.redcol.none.fl_str_mv http://purl.org/redcol/resource_type/ART
dc.type.version.none.fl_str_mv info:eu-repo/semantics/publishedVersion
format http://purl.org/coar/resource_type/c_6501
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.16925/di.v18i23.1292
dc.identifier.issn.spa.fl_str_mv 03005526
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/20.500.12494/41805
dc.identifier.bibliographicCitation.spa.fl_str_mv Hernandez JC,Latz E,Urcuqui S. HIV-1 induces the first signal to activate the NLRP3 inflammasome in monocyte-derived macrophages. Intervirology. 2013. 57. (1):p. 36-42. .
url https://doi.org/10.16925/di.v18i23.1292
https://hdl.handle.net/20.500.12494/41805
identifier_str_mv 03005526
Hernandez JC,Latz E,Urcuqui S. HIV-1 induces the first signal to activate the NLRP3 inflammasome in monocyte-derived macrophages. Intervirology. 2013. 57. (1):p. 36-42. .
dc.relation.ispartofjournal.spa.fl_str_mv Intervirology
dc.rights.accessrights.none.fl_str_mv info:eu-repo/semantics/closedAccess
dc.rights.coar.none.fl_str_mv http://purl.org/coar/access_right/c_14cb
eu_rights_str_mv closedAccess
rights_invalid_str_mv http://purl.org/coar/access_right/c_14cb
dc.format.extent.spa.fl_str_mv 42-36
dc.publisher.spa.fl_str_mv S. Karger AG
institution Universidad Cooperativa de Colombia
repository.name.fl_str_mv Repositorio Institucional Universidad Cooperativa de Colombia
repository.mail.fl_str_mv bdigital@metabiblioteca.com
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