HIV-1 induces the first signal to activate the NLRP3 inflammasome in monocyte-derived macrophages

Background/Aims: Inflammasomes are multimolecular complexes that regulate caspase-1. They act as sensors for endogenous and exogenous signals, and mediate the processing of pro-IL-1ß into its secreted, biologically active form. The NLRP3 inflammasome and IL-1ß are particularly interesting because they are required for efficient control of viral infections. Indeed, HIV-1 induces expression of NLRP3 and IL-1ß in healthy controls, but not in HIV-1-infected patients. Here we evaluate whether HIV-1 can induce activation of the NLRP3 inflammasome. Methods: Human primary monocyte-derived macrophages were infected with HIV-1 in the absence or presence of classical NLRP3 inflammasome activators, and IL-1ß release was assessed by ELISA. Results: HIV-1 initiates the priming signal for NLRP3 inflammasome activation through the NF-?B-associated pathway in human primary monocyte-derived macrophages. Furthermore, priming of NLRP3 activation in response to HIV-1 was independent of the viral envelope, since similar results were observed with HIV-1 and pseudotyped HIV-1 lacking the env gene. Conclusion: Our findings suggest that HIV-1 infection promotes IL-1ß secretion by inducing the first signal for NLRP3 inflammasome activation, a phenomenon that may contribute to AIDS progression. Copyright © 2013 S. Karger AG, Basel.

• Autores: Hernández López, Juan Carlos; Latz E.; Urcuqui-Inchima S.
• Tipo de recurso: Article of journal
• Fecha de publicación: 2013
• Institución: Universidad Cooperativa de Colombia
• Repositorio: Repositorio UCC
• OAI Identifier: oai:repository.ucc.edu.co:20.500.12494/41805
• Acceso en línea: https://doi.org/10.16925/di.v18i23.1292; https://hdl.handle.net/20.500.12494/41805
• Palabra clave: cryopyrin; immunoglobulin enhancer binding protein; inflammasome; interleukin 1beta; immunoglobulin enhancer binding protein; interleukin 1beta; article; controlled study; cytokine release; enzyme linked immunosorbent assay; human; human cell; Human immunodeficiency virus 1 infection; macrophage; priority journal; protein induction; signal transduction; virus envelope; Article; envelope gene; Human immunodeficiency virus 1; Human immunodeficiency virus 1 infection; macrophage; monocyte; nonhuman; Carrier Proteins; Cells; Cultured; Enzyme-Linked Immunosorbent Assay; HIV-1; Humans; Inflammasomes; Interleukin-1beta; Macrophages; Human immunodeficiency virus 1
• Rights: closedAccess
• License: http://purl.org/coar/access_right/c_14cb