Bromotyrosine derivatives from marine sponges inhibit the HIV-1 replication in vitro

Background: Human immunodeficiency virus type 1 (HIV-1) infection and Acquired immunodeficiency syndrome are mayor global public health issues. HIV-1 infection is now manageable as a chronic disease thanks to the development of antiretroviral therapy; however, the existence of HIV drug resistance and collateral effects have increased the search for therapeutic alternatives. Compounds of marine resources have been studied for their antiviral potential. Objectives: To evaluate the antiviral activity of isolated bromotyrosine-derivative compounds from the Colombian marine sponges, Verongula rigida and Aiolochoria crassa against HIV-1 infection in vitro. Methods: Cytotoxicity of 11 bromotyrosine-derivative compounds was determined by the MTT assay. Inhibition of HIV-1 replication was performed using the U373-MAGI cell line, which was infected with recombinant green fluorescent protein (GFP)-expressing viruses pseudotyped, in the presence or absence of the compounds. The percentage of infected cells was evaluated by of low cytometry. In addition, the inhibition of reverse transcription and nuclear import was determined by quantification of early and late reverse transcription products and 2-LTR circles, respectively, using quantitative PCR. Results: Aeroplysinin-1, purealidin B and 3-bromo-5-hydroxy-Omethyltyrosine inhibited the HIV-1 replication in a dose-dependent manner, with a median maximum percentage of inhibition of 74% (20 µM), 57% (80 µM) and 47% (80 µM), respectively. Importantly, none of these concentrations were cytotoxic. Aeroplysinin-1, 19-deoxyfistularin 3, purealidin B, fistularin 3 and 3-bromo-5-hydroxy-O-methyltyrosine inhibited the nuclear import efficiently; while 3,5-dibromo-N,N,N,O-tetramethyltyraminium, aeroplysinin-1, purealidin B, fistularin 3 and 3-bromo-5-hydroxy-Omethyltyrosine inhibited X4 HIV-1 cell entry with a median maximum percentage of inhibition ranging between 2 to 30%. Conclusions:Aeroplysinin-1, 19-deoxyfistularin 3, purealidin B, fistularin 3 and 3-bromo-5-hydroxy-O-methyltyrosine inhibited HIV replication at different steps. This study opens the possibility of chemically synthesizing these compounds and evaluating them as alternative therapies against HIV-1.

• Autores: Gómez-Archila L.G.; Zapata Builes, Wildeman; Galeano E.; Martínez A.; Díaz F.J.; Rugeles M.T.
• Tipo de recurso: Article of journal
• Fecha de publicación: 2014
• Institución: Universidad Cooperativa de Colombia
• Repositorio: Repositorio UCC
• OAI Identifier: oai:repository.ucc.edu.co:20.500.12494/42682
• Acceso en línea: https://www.scopus.com/inward/record.uri?eid=2-s2.0-84905048823&partnerID=40&md5=1ff7fb8925ea131bb2b0ac75a7fb5606; https://hdl.handle.net/20.500.12494/42682
• Palabra clave: 19 deoxyfistularin; 3 bromo 5 hydroxy O methyl tyrosine; 3 bromo N N N trimethyltyrosinium; 3,5 dibromo N N N O tetramethyltyraminium; 3,5 dibromo N N N trimethyltyraminium; 3,5 dibromo N N N trimethyltyrosinium; aeroplysinin 1; bromotyrosine derivative; dihydroxyaerothionin; fistularin 3; purealidin b; tyrosine derivative; unclassified drug; zidovudine; Aiolochoria crassa; article; controlled study; cytotoxicity; dose response; flow cytometry; human; human cell; Human immunodeficiency virus 1 infection; MTT assay; nonhuman; polymerase chain reaction; reverse transcription; sponge (Porifera); Verongula rigida; virus replication
• Rights: closedAccess
• License: http://purl.org/coar/access_right/c_14cb