Contamination of paper points used by students during preclinical and clinical endodontic procedures
Objective: To determine the presence of facultative anaerobic bacteria in the paper points used by students and to perform a pilot test to determine whether sterilization of these materials influences their absorption capacity. Material and Methods: This study consists of two phases. The first is a...
- Autores:
-
Angarita Díaz, María del Pilar
Universidad Cooperativa de Colombia
Angarita Díaz, Karen Mercedes
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2020
- Institución:
- Universidad Cooperativa de Colombia
- Repositorio:
- Repositorio UCC
- Idioma:
- OAI Identifier:
- oai:repository.ucc.edu.co:20.500.12494/20088
- Acceso en línea:
- https://hdl.handle.net/20.500.12494/20088
- Palabra clave:
- Microbiología
Contaminación
Bacteria
Anaeróbicos
Microbiology
Contamination
Bacteria
Anaerobic.
- Rights
- openAccess
- License
- Atribución
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Contamination of paper points used by students during preclinical and clinical endodontic procedures |
| title |
Contamination of paper points used by students during preclinical and clinical endodontic procedures |
| spellingShingle |
Contamination of paper points used by students during preclinical and clinical endodontic procedures Microbiología Contaminación Bacteria Anaeróbicos Microbiology Contamination Bacteria Anaerobic. |
| title_short |
Contamination of paper points used by students during preclinical and clinical endodontic procedures |
| title_full |
Contamination of paper points used by students during preclinical and clinical endodontic procedures |
| title_fullStr |
Contamination of paper points used by students during preclinical and clinical endodontic procedures |
| title_full_unstemmed |
Contamination of paper points used by students during preclinical and clinical endodontic procedures |
| title_sort |
Contamination of paper points used by students during preclinical and clinical endodontic procedures |
| dc.creator.fl_str_mv |
Angarita Díaz, María del Pilar Universidad Cooperativa de Colombia Angarita Díaz, Karen Mercedes |
| dc.contributor.author.none.fl_str_mv |
Angarita Díaz, María del Pilar Universidad Cooperativa de Colombia Angarita Díaz, Karen Mercedes |
| dc.subject.spa.fl_str_mv |
Microbiología Contaminación Bacteria Anaeróbicos |
| topic |
Microbiología Contaminación Bacteria Anaeróbicos Microbiology Contamination Bacteria Anaerobic. |
| dc.subject.other.spa.fl_str_mv |
Microbiology Contamination Bacteria Anaerobic. |
| description |
Objective: To determine the presence of facultative anaerobic bacteria in the paper points used by students and to perform a pilot test to determine whether sterilization of these materials influences their absorption capacity. Material and Methods: This study consists of two phases. The first is a descriptive phase where a representative sample of paper points (n = 72) was collected from the students and information about the points was voluntarily contributed. The points were placed in saline solution after they were collected, and mechanically shaken for 60 s. Then, 300 IU of the solution was seeded on blood agar in duplicates and incubated for 5 days under anaerobic conditions. The second phase was experimental, during which five paper points of each of the existing sizes were sterilized (Numbers: 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 70, and 80), and their capacity to absorb water was compared with that of the control or non-sterilized points. Results: The study determined that 22% (n=16) of the points were primarily contaminated by gram-positive bacilli, followed by gram-positive cocci, among which Staphylococcus epidermidis was identified. The presence of contamination was not significantly associated with the conditions of the paper points (p > 0.05). Furthermore, no significant effect (p>0.05) on the absorption capacity of these materials was detected in the sterilization test. Conclusion: Contamination was observed in the paper points used by the students, confirming the importance of implementing sterilization protocols. The sterilization protocols implemented in this study did not affect the absorption capacity of the points. |
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2020 |
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2020-08-26T20:58:00Z |
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2020-07-01 |
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Artículo |
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10.14295/bds.2020.v23i3.2055 |
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https://hdl.handle.net/20.500.12494/20088 |
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Angarita MP, Angarita KM. Contamination of paper points used by students during preclinical and clinical endodontic procedures. Braz Dent Sci. 2020. 23(3). doi: 10.14295/bds.2020.v23i3.2055 |
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21786011 10.14295/bds.2020.v23i3.2055 Angarita MP, Angarita KM. Contamination of paper points used by students during preclinical and clinical endodontic procedures. Braz Dent Sci. 2020. 23(3). doi: 10.14295/bds.2020.v23i3.2055 |
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https://bds.ict.unesp.br/index.php/cob/article/view/2055 |
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Brazilian Dental Science |
| dc.relation.references.spa.fl_str_mv |
World Health Organization. Infection prevention and control. [Internet]. WHO [cited 2019 Feb 12]. Available from: https://www.who.int/antimicrobial-resistance/global-action-plan/infection-prevention-control/en/ Centers for Disease Control and Prevention. Summary of infection prevention practices in dental settings: Basic expectations for safe care [Internet]. Atlanta, GA: Centers for Disease Control and Prevention, US Dept of Health and Human Services; 2016. [cited 2019 Feb 6]. Available from: https://www.cdc.gov/oralhealth/infectioncontrol/pdf/safe-care2.pdf Malmberg L, Björkner AE, Bergenholtz G. Establishment and maintenance of asepsis in endodontics - a review of the literature. Acta Odontol Scand. 2016;74(6):431–5. doi:10.1080/00016357.2016.1195508 Musani I, Goyal V, Singh A, Bhat C. Evaluation and comparison of biological cleaning efficacy of two endofiles and irrigants as judged by microbial quantification in primary teeth - an in vivo study. Int J Clin Pediatr Dent. 2009;2(3):15–22. doi:10.5005/jp-journals-10005-1013 Sakko M, Tjäderhane L, Rautemaa-Richardson R. Microbiology of root canal infections. Prim Dent J. 2016;5(2):84–9. doi:10.1308/205016816819304231 Aguiar CM, Torres T, Mendes DA, Farias B, Câmara AC. Effect of sterilization methods on the absorption capacity of absorbent paper points. Braz Dent Sci. 2012;15(1):27–32. doi: 10.14295/bds.2012.v15i1.737 Almeida MB, André MC, dos Santos P, de Oliveira T. Avaliação da contaminação de cones de papel absorvente. Rev Bras Odontol. 2010; 67(1):81–5. doi: 10.18363/rbo.v67n1.p.81 Pereira ER, Nabeshima CK, de Lima Machado ME. Analysis of contamination of endodontic absorbent paper points. Rev Odonto Cienc. 2011;26(1):56–60. doi: 10.1590/S1980-65232011000100013 Ximenes R, Marques F, da Silva J, Amaral G, Sassone LM. In vitro analysis of microbial contamination of paper points. RSBO. 2014;11(4):336–9. Pessoa de Andrade L, Chacon de Oliveira Conde N, Sponchiado Junior EC, Franco Marques AA, Pereira JV, Garcia LF. Contamination of absorbent paper points in clinical practice: a critical approach. Gen Dent. 2014;62(4):e38–e40. Avendaño A. Verificación de la esterilidad de las puntas de papel absorbente utilizadas en la terapia endodôntica [Internet]. [cited 2019 Jan 15]. Available from:http://www.carlosboveda.com/Odontologosfolder/odontoinvitadoold/odontoinvitado3.htm do Prado M, Duque TM, Gomes BPFA, Borges DO, Gusman HCS. Evaluation of cell pack paper points: a microbiological study. Dent Press Endod. 2012;2(2):42–6. Gajan EB, Aghazadeh M, Abashov R, Salem Milani A, Moosavi Z. Microbial flora of root canals of pulpally-infected teeth: enterococcus faecalis a prevalent species. J Dent Res Dent Clin Dent Prospects. 2009;3(1):24–7. doi:10.5681/joddd.2009.007 Shweta, Prakash SK. Dental abscess: a microbiological review. Dent Res J (Isfahan). 2013;10(5):585–91. Otto M. Staphylococcus epidermidis--the 'accidental' pathogen. Nat Rev Microbiol. 2009;7(8):555–67. doi:10.1038/nrmicro2182 O'Gara JP, Humphreys H. Staphylococcus epidermidis biofilms: importance and implications. J Med Microbiol. 2001;50(7):582–7. doi:10.1099/0022-1317-50-7-582 Otto M. Staphylococcus epidermidis pathogenesis. Methods Mol Biol. 2014;1106:17–31. doi: 10.1007/978-1-62703-736-5_2 Kubo CH, Gomes APM, Jorge AOC. Influência dos métodos de esterilização na capacidade e velocidade de absorção de diferentes marcas comerciais de cones de papel absorvente para endodontia. Rev Odontol UNESP 2000;29(1/2):113-27. Victorino FR, Lukiantchuk M, Garcia LB, Bramante CM, Moraes IG, Hidalgo MM. Capacidade de absorção e toxicidade de cones de papel após esterilização. RGO. 2008;56(4):411-5. |
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Angarita Díaz, María del PilarUniversidad Cooperativa de ColombiaAngarita Díaz, Karen Mercedes232020-08-26T20:58:00Z2020-08-26T20:58:00Z2020-07-012178601110.14295/bds.2020.v23i3.2055https://hdl.handle.net/20.500.12494/20088Angarita MP, Angarita KM. Contamination of paper points used by students during preclinical and clinical endodontic procedures. Braz Dent Sci. 2020. 23(3). doi: 10.14295/bds.2020.v23i3.2055Objective: To determine the presence of facultative anaerobic bacteria in the paper points used by students and to perform a pilot test to determine whether sterilization of these materials influences their absorption capacity. Material and Methods: This study consists of two phases. The first is a descriptive phase where a representative sample of paper points (n = 72) was collected from the students and information about the points was voluntarily contributed. The points were placed in saline solution after they were collected, and mechanically shaken for 60 s. Then, 300 IU of the solution was seeded on blood agar in duplicates and incubated for 5 days under anaerobic conditions. The second phase was experimental, during which five paper points of each of the existing sizes were sterilized (Numbers: 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 70, and 80), and their capacity to absorb water was compared with that of the control or non-sterilized points. Results: The study determined that 22% (n=16) of the points were primarily contaminated by gram-positive bacilli, followed by gram-positive cocci, among which Staphylococcus epidermidis was identified. The presence of contamination was not significantly associated with the conditions of the paper points (p > 0.05). Furthermore, no significant effect (p>0.05) on the absorption capacity of these materials was detected in the sterilization test. Conclusion: Contamination was observed in the paper points used by the students, confirming the importance of implementing sterilization protocols. The sterilization protocols implemented in this study did not affect the absorption capacity of the points.http://scienti.colciencias.gov.co:8081/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0001561382https://orcid.org/0000-0002-5435-3456GIOMETmaria.angaritad@campusucc.edu.co8Universidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Programa de Odontología, Villavicencio, Colombia, 00000OdontologíaVillavicenciohttps://bds.ict.unesp.br/index.php/cob/article/view/2055Brazilian Dental ScienceWorld Health Organization. Infection prevention and control. [Internet]. WHO [cited 2019 Feb 12]. Available from: https://www.who.int/antimicrobial-resistance/global-action-plan/infection-prevention-control/en/Centers for Disease Control and Prevention. Summary of infection prevention practices in dental settings: Basic expectations for safe care [Internet]. Atlanta, GA: Centers for Disease Control and Prevention, US Dept of Health and Human Services; 2016. [cited 2019 Feb 6]. Available from: https://www.cdc.gov/oralhealth/infectioncontrol/pdf/safe-care2.pdfMalmberg L, Björkner AE, Bergenholtz G. Establishment and maintenance of asepsis in endodontics - a review of the literature. Acta Odontol Scand. 2016;74(6):431–5. doi:10.1080/00016357.2016.1195508Musani I, Goyal V, Singh A, Bhat C. Evaluation and comparison of biological cleaning efficacy of two endofiles and irrigants as judged by microbial quantification in primary teeth - an in vivo study. Int J Clin Pediatr Dent. 2009;2(3):15–22. doi:10.5005/jp-journals-10005-1013Sakko M, Tjäderhane L, Rautemaa-Richardson R. Microbiology of root canal infections. Prim Dent J. 2016;5(2):84–9. doi:10.1308/205016816819304231Aguiar CM, Torres T, Mendes DA, Farias B, Câmara AC. Effect of sterilization methods on the absorption capacity of absorbent paper points. Braz Dent Sci. 2012;15(1):27–32. doi: 10.14295/bds.2012.v15i1.737Almeida MB, André MC, dos Santos P, de Oliveira T. Avaliação da contaminação de cones de papel absorvente. Rev Bras Odontol. 2010; 67(1):81–5. doi: 10.18363/rbo.v67n1.p.81Pereira ER, Nabeshima CK, de Lima Machado ME. Analysis of contamination of endodontic absorbent paper points. Rev Odonto Cienc. 2011;26(1):56–60. doi: 10.1590/S1980-65232011000100013Ximenes R, Marques F, da Silva J, Amaral G, Sassone LM. In vitro analysis of microbial contamination of paper points. RSBO. 2014;11(4):336–9.Pessoa de Andrade L, Chacon de Oliveira Conde N, Sponchiado Junior EC, Franco Marques AA, Pereira JV, Garcia LF. Contamination of absorbent paper points in clinical practice: a critical approach. Gen Dent. 2014;62(4):e38–e40.Avendaño A. Verificación de la esterilidad de las puntas de papel absorbente utilizadas en la terapia endodôntica [Internet]. [cited 2019 Jan 15]. Available from:http://www.carlosboveda.com/Odontologosfolder/odontoinvitadoold/odontoinvitado3.htmdo Prado M, Duque TM, Gomes BPFA, Borges DO, Gusman HCS. Evaluation of cell pack paper points: a microbiological study. Dent Press Endod. 2012;2(2):42–6.Gajan EB, Aghazadeh M, Abashov R, Salem Milani A, Moosavi Z. Microbial flora of root canals of pulpally-infected teeth: enterococcus faecalis a prevalent species. J Dent Res Dent Clin Dent Prospects. 2009;3(1):24–7. doi:10.5681/joddd.2009.007Shweta, Prakash SK. Dental abscess: a microbiological review. Dent Res J (Isfahan). 2013;10(5):585–91.Otto M. Staphylococcus epidermidis--the 'accidental' pathogen. Nat Rev Microbiol. 2009;7(8):555–67. doi:10.1038/nrmicro2182O'Gara JP, Humphreys H. Staphylococcus epidermidis biofilms: importance and implications. J Med Microbiol. 2001;50(7):582–7. doi:10.1099/0022-1317-50-7-582Otto M. Staphylococcus epidermidis pathogenesis. Methods Mol Biol. 2014;1106:17–31. doi: 10.1007/978-1-62703-736-5_2Kubo CH, Gomes APM, Jorge AOC. Influência dos métodos de esterilização na capacidade e velocidade de absorção de diferentes marcas comerciais de cones de papel absorvente para endodontia. Rev Odontol UNESP 2000;29(1/2):113-27.Victorino FR, Lukiantchuk M, Garcia LB, Bramante CM, Moraes IG, Hidalgo MM. Capacidade de absorção e toxicidade de cones de papel após esterilização. RGO. 2008;56(4):411-5.MicrobiologíaContaminaciónBacteriaAnaeróbicosMicrobiologyContaminationBacteriaAnaerobic.Contamination of paper points used by students during preclinical and clinical endodontic proceduresArtículohttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAtribucióninfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2PublicationORIGINAL2020_Contamination_paper_points_students.pdf2020_Contamination_paper_points_students.pdfArtículoapplication/pdf530133https://repository.ucc.edu.co/bitstreams/840f706b-f7c8-470a-b13b-3983ae3a4e53/download7b6fcc894a8c160bda2a9847b7d69959MD51Licencia de uso RI Ver5 - 20-05-2020.doc (1) Contaminacion conos de papel.pdfLicencia de uso RI Ver5 - 20-05-2020.doc (1) Contaminacion conos de papel.pdfLicencia de 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