Protocol for the Collection of Nail Matrix Biopsies in Corpses
Introduction: The gold standard for the diagnosis of subungual melanocytic lesions is the histopathological study. Parameters such as number, morphological characteristics and location of melanocytes in the nail apparatus are essential to differentiate benign lesions from nail melanoma, malignant ne...
- Autores:
-
Hernández Rubio, Mario Alberto
Vargas Carreño, Elga Johanna
Abaunza Chagin, María Claudia
Fernández Moya, David Andrés
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2016
- Institución:
- Universidad Cooperativa de Colombia
- Repositorio:
- Repositorio UCC
- Idioma:
- spa
- OAI Identifier:
- oai:repository.ucc.edu.co:20.500.12494/44339
- Acceso en línea:
- https://revistas.ucc.edu.co/index.php/ml/article/view/1779
https://hdl.handle.net/20.500.12494/44339
- Palabra clave:
- biopsy
fingers
toes
dissection
melanocytes
nails
biopsia
dedos
disección
melanocitos
uñas
biópsia
dedos
dissecção
melanócitos
unhas
- Rights
- openAccess
- License
- Derechos de autor 2017 Colombia Forense
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| dc.title.eng.fl_str_mv |
Protocol for the Collection of Nail Matrix Biopsies in Corpses |
| dc.title.spa.fl_str_mv |
Protocolo para la toma de biopsias de matriz ungular en cadáveres |
| dc.title.por.fl_str_mv |
Protocolo para a realização de biópsias de matriz ungueal em cadáveres |
| title |
Protocol for the Collection of Nail Matrix Biopsies in Corpses |
| spellingShingle |
Protocol for the Collection of Nail Matrix Biopsies in Corpses biopsy fingers toes dissection melanocytes nails biopsia dedos disección melanocitos uñas biópsia dedos dissecção melanócitos unhas |
| title_short |
Protocol for the Collection of Nail Matrix Biopsies in Corpses |
| title_full |
Protocol for the Collection of Nail Matrix Biopsies in Corpses |
| title_fullStr |
Protocol for the Collection of Nail Matrix Biopsies in Corpses |
| title_full_unstemmed |
Protocol for the Collection of Nail Matrix Biopsies in Corpses |
| title_sort |
Protocol for the Collection of Nail Matrix Biopsies in Corpses |
| dc.creator.fl_str_mv |
Hernández Rubio, Mario Alberto Vargas Carreño, Elga Johanna Abaunza Chagin, María Claudia Fernández Moya, David Andrés |
| dc.contributor.author.none.fl_str_mv |
Hernández Rubio, Mario Alberto Vargas Carreño, Elga Johanna Abaunza Chagin, María Claudia Fernández Moya, David Andrés |
| dc.subject.eng.fl_str_mv |
biopsy fingers toes dissection melanocytes nails |
| topic |
biopsy fingers toes dissection melanocytes nails biopsia dedos disección melanocitos uñas biópsia dedos dissecção melanócitos unhas |
| dc.subject.spa.fl_str_mv |
biopsia dedos disección melanocitos uñas |
| dc.subject.por.fl_str_mv |
biópsia dedos dissecção melanócitos unhas |
| description |
Introduction: The gold standard for the diagnosis of subungual melanocytic lesions is the histopathological study. Parameters such as number, morphological characteristics and location of melanocytes in the nail apparatus are essential to differentiate benign lesions from nail melanoma, malignant neoplasm of aggressive behavior and poor prognosis. In our country, there have been no studies aimed at quantifying melanocytes in the normal nail matrix or pigmented lesions of the nails. For this reason, a research project was carried out to determine the mean number of melanocytes in the normal nail matrix, including the creation of a protocol for the collection of nail matrix biopsies in corpses. Materials and methods: Nail matrix biopsies of the first finger and toe, of the right hand and foot,were performed on 41 Colombian mestizo cadavers without macroscopic evidence of nail lesions following the sampling protocol described in this article. Results and discussion: The protocol for collecting nail matrix biopsies in corpses consists of five steps: nail inspection, dissection and avulsion of the nail plate, collection of two longitudinal nail matrix biopsies,repositioning and fixing of the plate, and fixing of the nail folds. Conclusions: The protocol created allows to obtain nail matrix biopsies without important aesthetic alterations, while providing good quality samples. |
| publishDate |
2016 |
| dc.date.accessioned.none.fl_str_mv |
2022-03-02T16:45:24Z |
| dc.date.available.none.fl_str_mv |
2022-03-02T16:45:24Z |
| dc.date.none.fl_str_mv |
2016-10-20 |
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Artículo |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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http://purl.org/coar/resource_type/c_6501 |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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info:eu-repo/semantics/article |
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http://purl.org/redcol/resource_type/ART |
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https://revistas.ucc.edu.co/index.php/ml/article/view/1779 10.16925/cf.v3i2.1779 |
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https://hdl.handle.net/20.500.12494/44339 |
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https://revistas.ucc.edu.co/index.php/ml/article/view/1779 https://hdl.handle.net/20.500.12494/44339 |
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10.16925/cf.v3i2.1779 |
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spa |
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spa |
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https://revistas.ucc.edu.co/index.php/ml/article/view/1779/2043 |
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Derechos de autor 2017 Colombia Forense |
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info:eu-repo/semantics/openAccess |
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http://purl.org/coar/access_right/c_abf2 |
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application/pdf |
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Universidad Cooperativa de Colombia / Instituto Nacional de Medicina Legal y Ciencias Forenses |
| dc.source.eng.fl_str_mv |
Colombia Forense; Vol. 3 No. 2; 75-86 |
| dc.source.spa.fl_str_mv |
Colombia Forense; Vol. 3 Núm. 2; 75-86 |
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Colombia Forense; v. 3 n. 2; 75-86 |
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2145-9649 2145-0684 |
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Universidad Cooperativa de Colombia |
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Repositorio Institucional Universidad Cooperativa de Colombia |
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Hernández Rubio, Mario AlbertoVargas Carreño, Elga JohannaAbaunza Chagin, María ClaudiaFernández Moya, David Andrés2016-10-202022-03-02T16:45:24Z2022-03-02T16:45:24Zhttps://revistas.ucc.edu.co/index.php/ml/article/view/177910.16925/cf.v3i2.1779https://hdl.handle.net/20.500.12494/44339Introduction: The gold standard for the diagnosis of subungual melanocytic lesions is the histopathological study. Parameters such as number, morphological characteristics and location of melanocytes in the nail apparatus are essential to differentiate benign lesions from nail melanoma, malignant neoplasm of aggressive behavior and poor prognosis. In our country, there have been no studies aimed at quantifying melanocytes in the normal nail matrix or pigmented lesions of the nails. For this reason, a research project was carried out to determine the mean number of melanocytes in the normal nail matrix, including the creation of a protocol for the collection of nail matrix biopsies in corpses. Materials and methods: Nail matrix biopsies of the first finger and toe, of the right hand and foot,were performed on 41 Colombian mestizo cadavers without macroscopic evidence of nail lesions following the sampling protocol described in this article. Results and discussion: The protocol for collecting nail matrix biopsies in corpses consists of five steps: nail inspection, dissection and avulsion of the nail plate, collection of two longitudinal nail matrix biopsies,repositioning and fixing of the plate, and fixing of the nail folds. Conclusions: The protocol created allows to obtain nail matrix biopsies without important aesthetic alterations, while providing good quality samples.Introducción. El estándar de oro para el diagnóstico de las lesiones melanocíticas ungulares es el estudio histopatológico. Parámetros como el número, características morfológicas y ubicación de los melanocitos en el aparato ungular, son esenciales para diferenciar las lesiones benignas del melanoma ungular, neoplasia maligna de comportamiento agresivo y pobre pronóstico. En nuestro país no se han realizado estudios de cuantificación de melanocitos en la matriz ungular normal ni en lesiones pigmentadas de las uñas. Por esta razón, se desarrolló un protocolo de toma de biopsias de matriz ungular en cadáveres sin generar alteraciones estéticas importantes y proporcionando muestras de buena calidad que permitan una evaluación histológica adecuada. Los principales pasos del protocolo de toma de biopsias son: inspección de la uña, disección y avulsión de la placa ungular, toma de dos biopsias longitudinales de la matriz ungular, reposicionamiento y fijación de la lámina y fijación de los pliegues ungulares.Introdução: o critério de referência para o diagnóstico das lesões melanocíticas ungueais é o estudo histopatológico. Parâmetros como o número, as características morfológicas e o posicionamento dos melanócitos no aparelho ungueal são essenciais para diferenciar as lesões benignas do melanoma ungueal, neoplasia maligna de comportamento agressivo e pobre prognóstico. Na Colômbia, não vêm sendo realizados estudos de quantificação de melanócitos na matriz ungueal normal nem em lesões pigmentadas das unhas. Por essa razão, foi realizado um projeto de pesquisa para determinar a média de melanócitos na matriz ungueal normal, o que incluiu o desenvolvimento de um protocolo de tomada de biópsias de matriz ungueal em cadáveres. Métodos e materiais:foram realizadas biópsias da matriz ungueal do primeiro dedo, tanto do pé quanto da mão direita, de 41 cadáveres de nacionalidade colombiana, raça mestiça e sem evidência macroscópica de lesões ungueais seguindo o protocolo de tomada descrito neste artigo. Resultados e discussão o protocolo de realização de biópsias de matriz ungueal em cadáveres contém cinco passos: inspeção da unha, dissecção e avulsão da placa ungueal, realização de duas biópsias longitudinais da matriz ungueal, reposicionamento e fixação da lâmina e fixação das pregas ungueais. Conclusões: o protocolo desenvolvido permite obter biópsias da matriz ungueal sem gerar alterações estéticas importantes e proporciona amostras de boa qualidade.application/pdfspaUniversidad Cooperativa de Colombia / Instituto Nacional de Medicina Legal y Ciencias Forenseshttps://revistas.ucc.edu.co/index.php/ml/article/view/1779/2043Derechos de autor 2017 Colombia Forenseinfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Colombia Forense; Vol. 3 No. 2; 75-86Colombia Forense; Vol. 3 Núm. 2; 75-86Colombia Forense; v. 3 n. 2; 75-862145-96492145-0684biopsyfingerstoesdissectionmelanocytesnailsbiopsiadedosdisecciónmelanocitosuñasbiópsiadedosdissecçãomelanócitosunhasProtocol for the Collection of Nail Matrix Biopsies in CorpsesProtocolo para la toma de biopsias de matriz ungular en cadáveresProtocolo para a realização de biópsias de matriz ungueal em cadáveresArtículohttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionPublication20.500.12494/44339oai:repository.ucc.edu.co:20.500.12494/443392024-07-16 13:30:32.094metadata.onlyhttps://repository.ucc.edu.coRepositorio Institucional Universidad Cooperativa de Colombiabdigital@metabiblioteca.com |