Molecular characterization of mosquitoes (Diptera: Culicidae) from the Colombian rainforest
A few studies have carried out the taxonomic and molecular characterization of sylvatic mosquito species in Latin America, where some species have been incriminated as vectors for arboviruses and parasites transmission. The present study reports the molecular characterization of mosquito species in...
- Autores:
-
Muñoz Gamba, Andrew S.
Laiton Donato, Katherine
Perdomo Balaguera, Erick
Castro, Lyda R.
Usme Ciro, José Aldemar
Parra Henao, Gabriel
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2021
- Institución:
- Universidad Cooperativa de Colombia
- Repositorio:
- Repositorio UCC
- Idioma:
- OAI Identifier:
- oai:repository.ucc.edu.co:20.500.12494/46182
- Acceso en línea:
- https://hdl.handle.net/20.500.12494/46182
- Palabra clave:
- Culicidae
Codigo de barras de ADN
COI
Taxonomia
Culicidae
DNA barcoding
COI
Taxonomy
- Rights
- openAccess
- License
- Atribución
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dc.title.spa.fl_str_mv |
Molecular characterization of mosquitoes (Diptera: Culicidae) from the Colombian rainforest |
title |
Molecular characterization of mosquitoes (Diptera: Culicidae) from the Colombian rainforest |
spellingShingle |
Molecular characterization of mosquitoes (Diptera: Culicidae) from the Colombian rainforest Culicidae Codigo de barras de ADN COI Taxonomia Culicidae DNA barcoding COI Taxonomy |
title_short |
Molecular characterization of mosquitoes (Diptera: Culicidae) from the Colombian rainforest |
title_full |
Molecular characterization of mosquitoes (Diptera: Culicidae) from the Colombian rainforest |
title_fullStr |
Molecular characterization of mosquitoes (Diptera: Culicidae) from the Colombian rainforest |
title_full_unstemmed |
Molecular characterization of mosquitoes (Diptera: Culicidae) from the Colombian rainforest |
title_sort |
Molecular characterization of mosquitoes (Diptera: Culicidae) from the Colombian rainforest |
dc.creator.fl_str_mv |
Muñoz Gamba, Andrew S. Laiton Donato, Katherine Perdomo Balaguera, Erick Castro, Lyda R. Usme Ciro, José Aldemar Parra Henao, Gabriel |
dc.contributor.author.none.fl_str_mv |
Muñoz Gamba, Andrew S. Laiton Donato, Katherine Perdomo Balaguera, Erick Castro, Lyda R. Usme Ciro, José Aldemar Parra Henao, Gabriel |
dc.subject.spa.fl_str_mv |
Culicidae Codigo de barras de ADN COI Taxonomia |
topic |
Culicidae Codigo de barras de ADN COI Taxonomia Culicidae DNA barcoding COI Taxonomy |
dc.subject.other.spa.fl_str_mv |
Culicidae DNA barcoding COI Taxonomy |
description |
A few studies have carried out the taxonomic and molecular characterization of sylvatic mosquito species in Latin America, where some species have been incriminated as vectors for arboviruses and parasites transmission. The present study reports the molecular characterization of mosquito species in the Sierra Nevada de Santa Marta, a natural ecosystem in the Northern coast of Colombia. Manual capture methods were used to collect mosquitoes, and the specimens were identified via classical taxonomy. The COI marker was used for species confirmation, and phylogenetic analysis was performed using the neighbor-joining method, with the Kimura-2-Parameters model. Aedes serratus , Psorophora ferox , Johnbelkinia ulopus , Sabethes chloropterus , Sabethes cyaneus , Wyeomyia aporonoma , Wyeomyia pseudopecten , Wyeomyia ulocoma and Wyeomyia luteoventralis were identified. We assessed the genetic variability of mosquitoes in this area and phylogenetic reconstructions allowed the identification at the species level. Classical and molecular taxonomy demonstrated to be useful and complementary when morphological characteristics are not well preserved, or the taxonomic group is not represented in public molecular databases. |
publishDate |
2021 |
dc.date.issued.none.fl_str_mv |
2021-03-24 |
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2022-08-19T23:43:35Z |
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2022-08-19T23:43:35Z |
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Artículos Científicos |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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1678-9946 |
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10.1590/S1678-9946202163024 |
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https://hdl.handle.net/20.500.12494/46182 |
dc.identifier.bibliographicCitation.spa.fl_str_mv |
Muñoz-Gamba AS, Laiton-Donato K, Perdomo-Balaguera E, Castro LR, Usme-Ciro JA, Parra-Henao G. Molecular characterization of mosquitoes (Diptera: Culicidae) from the Colombian rainforest. Rev Inst Med Trop Sao Paulo. 2021 Mar 24;63:e24. doi: 10.1590/S1678-9946202163024.https://repository.ucc.edu.co/handle/20.500.12494/46182 |
identifier_str_mv |
1678-9946 10.1590/S1678-9946202163024 Muñoz-Gamba AS, Laiton-Donato K, Perdomo-Balaguera E, Castro LR, Usme-Ciro JA, Parra-Henao G. Molecular characterization of mosquitoes (Diptera: Culicidae) from the Colombian rainforest. Rev Inst Med Trop Sao Paulo. 2021 Mar 24;63:e24. doi: 10.1590/S1678-9946202163024.https://repository.ucc.edu.co/handle/20.500.12494/46182 |
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https://hdl.handle.net/20.500.12494/46182 |
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https://www.scielo.br/j/rimtsp/a/WkdHTQm59wsGJk4DLhLJ7gD/?lang=en |
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Revista do Instituto de Medicina Tropical de São Paulo |
dc.relation.references.spa.fl_str_mv |
Harbach RE. The Culicidae (Diptera): a review of taxonomy, classification and phylogeny. Zootaxa. 2007;16681:591-638. World Health Organization. Vector-borne diseases: key facts. [cited 2021 Jan 21]. Available from: https://www.who.int/en/ news-room/fact-sheets/detail/vector-borne-diseases Vasilakis N, Cardosa J, Hanley KA, Holmes EC, Weaver SC. Fever from the forest: prospects for the continued emergence of sylvatic dengue virus and its impact on public health. Nat Rev Microbiol. 2011;9:532-41. Cova GP, Tallaferro DE, Sutil OE. General principles of entomology. Montevideo: Fundación Venezolana para la Salud y la Educación; 1974. International Commission on Zoological Nomenclature. International code of zoological nomenclature. 4th ed. London: International Trust for Zoological Nomenclature; 1999. [cited 2021 Jan 21] Available from: https://www.iczn.org/the-code/ the-international-code-of-zoological-nomenclature/the-codeonline/ Kress WJ, Garcia-Robledo C, Uriarte M, Erickson DL. DNA barcodes for ecology, evolution, and conservation. Trends Ecol Evol. 2015;30:25-35. Shah MA, Khan AA, Khan HZ. DNA barcoding-applications in insect ecology. Indian J Ecol. 2015;42:287-94 Arnot DE, Roper C, Bayoumi RA. Digital codes from hypervariable tandemly repeated DNA sequences in the Plasmodium falciparum circumsporozoite gene can genetically barcode isolates. Mol Biochem Parasitol. 1993;61:15-24. Hebert PD, Ratnasingham S, deWaard JR. Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species. Proc Biol Sci. 2003;270 Suppl 1:S96-9. Naranjo-Diaz N, Rosero DA, Rua-Uribe G, Luckhart S, Correa MM. Abundance, behavior and entomological inoculation rates of anthropophilic anophelines from a primary Colombian malaria endemic area. Parasit Vectors. 2013;6:61. Rozo-Lopez P, Mengual X. Mosquito species (Diptera, Culicidae) in three ecosystems from the Colombian Andes: identification through DNA barcoding and adult morphology. Zookeys. 2015;513:39-64. Sánchez J, Galeano, Y, Rosero DA, Naranjo NJ, Correa MM. Confirmación molecular de especie en especímenes Anopheles recolectados en el corregimiento de Juan José, Puerto Libertador, Córdoba, Colombia. Hechos Microbiol. 2011;2:19- 28. Vidales H, Buitrago B, Sanín LH, Morales A, Groot H. Estudio de un brote epidémico de fiebre amarilla selvática en el pie de monte de La Sierra Nevada de Santa Marta, 1979. Biomédica. 1981;1:171-86. Balaguera L, Cantillo J, Camargo LF, Gómez A, Jiménez M, Laverde GN, et al. Plan de manejo Parque Nacional Natural Sierra Nevada de Santa Marta. Santa Marta: Unidad Administrativa Especial del Sistema de Parques Nacionales Naturales; 2015. [cited 2021 Jan 21]. Available from: https://www.parquesnacionales.gov.co/portal/wp-content/ uploads/2013/12/parqueSierraNevadadeSantaMarta.pdf Colombia. Banco de la República. Sierra Nevada de Santa Marta: economía de sus recursos naturales. [cited 2021 Jan 21]. Available from: https://www.banrep.gov.co/es/sierra-nevadasanta-marta-economia-sus-recursos-naturales |
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dc.publisher.spa.fl_str_mv |
Universidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Medicina, Santa Marta Thelma Suely Okay |
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Medicina |
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Santa Marta |
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Universidad Cooperativa de Colombia |
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Muñoz Gamba, Andrew S.Laiton Donato, KatherinePerdomo Balaguera, ErickCastro, Lyda R.Usme Ciro, José AldemarParra Henao, Gabriel632022-08-19T23:43:35Z2022-08-19T23:43:35Z2021-03-241678-994610.1590/S1678-9946202163024https://hdl.handle.net/20.500.12494/46182Muñoz-Gamba AS, Laiton-Donato K, Perdomo-Balaguera E, Castro LR, Usme-Ciro JA, Parra-Henao G. Molecular characterization of mosquitoes (Diptera: Culicidae) from the Colombian rainforest. Rev Inst Med Trop Sao Paulo. 2021 Mar 24;63:e24. doi: 10.1590/S1678-9946202163024.https://repository.ucc.edu.co/handle/20.500.12494/46182A few studies have carried out the taxonomic and molecular characterization of sylvatic mosquito species in Latin America, where some species have been incriminated as vectors for arboviruses and parasites transmission. The present study reports the molecular characterization of mosquito species in the Sierra Nevada de Santa Marta, a natural ecosystem in the Northern coast of Colombia. Manual capture methods were used to collect mosquitoes, and the specimens were identified via classical taxonomy. The COI marker was used for species confirmation, and phylogenetic analysis was performed using the neighbor-joining method, with the Kimura-2-Parameters model. Aedes serratus , Psorophora ferox , Johnbelkinia ulopus , Sabethes chloropterus , Sabethes cyaneus , Wyeomyia aporonoma , Wyeomyia pseudopecten , Wyeomyia ulocoma and Wyeomyia luteoventralis were identified. We assessed the genetic variability of mosquitoes in this area and phylogenetic reconstructions allowed the identification at the species level. Classical and molecular taxonomy demonstrated to be useful and complementary when morphological characteristics are not well preserved, or the taxonomic group is not represented in public molecular databases.https://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0000318507https://orcid.org/0000-0002-8093-0544https://scienti.minciencias.gov.co/gruplac/jsp/visualiza/visualizagr.jsp?nro=00000000008981jose.usmec@campusucc.edu.cohttps://scholar.google.com.co/citations?user=cU2KyT4AAAAJ&hl=en11Universidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Medicina, Santa MartaThelma Suely OkayMedicinaSanta Martahttps://www.scielo.br/j/rimtsp/a/WkdHTQm59wsGJk4DLhLJ7gD/?lang=enRevista do Instituto de Medicina Tropical de São PauloHarbach RE. The Culicidae (Diptera): a review of taxonomy, classification and phylogeny. Zootaxa. 2007;16681:591-638.World Health Organization. Vector-borne diseases: key facts. [cited 2021 Jan 21]. Available from: https://www.who.int/en/ news-room/fact-sheets/detail/vector-borne-diseasesVasilakis N, Cardosa J, Hanley KA, Holmes EC, Weaver SC. Fever from the forest: prospects for the continued emergence of sylvatic dengue virus and its impact on public health. Nat Rev Microbiol. 2011;9:532-41.Cova GP, Tallaferro DE, Sutil OE. General principles of entomology. Montevideo: Fundación Venezolana para la Salud y la Educación; 1974.International Commission on Zoological Nomenclature. International code of zoological nomenclature. 4th ed. London: International Trust for Zoological Nomenclature; 1999. [cited 2021 Jan 21] Available from: https://www.iczn.org/the-code/ the-international-code-of-zoological-nomenclature/the-codeonline/Kress WJ, Garcia-Robledo C, Uriarte M, Erickson DL. DNA barcodes for ecology, evolution, and conservation. Trends Ecol Evol. 2015;30:25-35.Shah MA, Khan AA, Khan HZ. DNA barcoding-applications in insect ecology. Indian J Ecol. 2015;42:287-94Arnot DE, Roper C, Bayoumi RA. Digital codes from hypervariable tandemly repeated DNA sequences in the Plasmodium falciparum circumsporozoite gene can genetically barcode isolates. Mol Biochem Parasitol. 1993;61:15-24.Hebert PD, Ratnasingham S, deWaard JR. Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species. Proc Biol Sci. 2003;270 Suppl 1:S96-9.Naranjo-Diaz N, Rosero DA, Rua-Uribe G, Luckhart S, Correa MM. Abundance, behavior and entomological inoculation rates of anthropophilic anophelines from a primary Colombian malaria endemic area. Parasit Vectors. 2013;6:61.Rozo-Lopez P, Mengual X. Mosquito species (Diptera, Culicidae) in three ecosystems from the Colombian Andes: identification through DNA barcoding and adult morphology. Zookeys. 2015;513:39-64.Sánchez J, Galeano, Y, Rosero DA, Naranjo NJ, Correa MM. Confirmación molecular de especie en especímenes Anopheles recolectados en el corregimiento de Juan José, Puerto Libertador, Córdoba, Colombia. Hechos Microbiol. 2011;2:19- 28.Vidales H, Buitrago B, Sanín LH, Morales A, Groot H. Estudio de un brote epidémico de fiebre amarilla selvática en el pie de monte de La Sierra Nevada de Santa Marta, 1979. Biomédica. 1981;1:171-86.Balaguera L, Cantillo J, Camargo LF, Gómez A, Jiménez M, Laverde GN, et al. Plan de manejo Parque Nacional Natural Sierra Nevada de Santa Marta. Santa Marta: Unidad Administrativa Especial del Sistema de Parques Nacionales Naturales; 2015. [cited 2021 Jan 21]. Available from: https://www.parquesnacionales.gov.co/portal/wp-content/ uploads/2013/12/parqueSierraNevadadeSantaMarta.pdfColombia. Banco de la República. Sierra Nevada de Santa Marta: economía de sus recursos naturales. [cited 2021 Jan 21]. Available from: https://www.banrep.gov.co/es/sierra-nevadasanta-marta-economia-sus-recursos-naturalesCulicidaeCodigo de barras de ADNCOITaxonomiaCulicidaeDNA barcodingCOITaxonomyMolecular characterization of mosquitoes (Diptera: Culicidae) from the Colombian rainforestArtículos Científicoshttp://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAtribucióninfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2PublicationLICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repository.ucc.edu.co/bitstreams/3dc92251-6416-4bc9-9558-3f1ad634e952/download8a4605be74aa9ea9d79846c1fba20a33MD54ORIGINAL32. Molecular characterization of mosquitoes (Diptera Culicidae) from the Colombian rainforest. Munoz-Gamba et al 2021.pdf32. Molecular characterization of mosquitoes (Diptera Culicidae) from the Colombian rainforest. 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