Molecular characterization of mosquitoes (Diptera: Culicidae) from the Colombian rainforest

A few studies have carried out the taxonomic and molecular characterization of sylvatic mosquito species in Latin America, where some species have been incriminated as vectors for arboviruses and parasites transmission. The present study reports the molecular characterization of mosquito species in...

Full description

Autores:
Muñoz Gamba, Andrew S.
Laiton Donato, Katherine
Perdomo Balaguera, Erick
Castro, Lyda R.
Usme Ciro, José Aldemar
Parra Henao, Gabriel
Tipo de recurso:
Article of investigation
Fecha de publicación:
2021
Institución:
Universidad Cooperativa de Colombia
Repositorio:
Repositorio UCC
Idioma:
OAI Identifier:
oai:repository.ucc.edu.co:20.500.12494/46182
Acceso en línea:
https://hdl.handle.net/20.500.12494/46182
Palabra clave:
Culicidae
Codigo de barras de ADN
COI
Taxonomia
Culicidae
DNA barcoding
COI
Taxonomy
Rights
openAccess
License
Atribución
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dc.title.spa.fl_str_mv Molecular characterization of mosquitoes (Diptera: Culicidae) from the Colombian rainforest
title Molecular characterization of mosquitoes (Diptera: Culicidae) from the Colombian rainforest
spellingShingle Molecular characterization of mosquitoes (Diptera: Culicidae) from the Colombian rainforest
Culicidae
Codigo de barras de ADN
COI
Taxonomia
Culicidae
DNA barcoding
COI
Taxonomy
title_short Molecular characterization of mosquitoes (Diptera: Culicidae) from the Colombian rainforest
title_full Molecular characterization of mosquitoes (Diptera: Culicidae) from the Colombian rainforest
title_fullStr Molecular characterization of mosquitoes (Diptera: Culicidae) from the Colombian rainforest
title_full_unstemmed Molecular characterization of mosquitoes (Diptera: Culicidae) from the Colombian rainforest
title_sort Molecular characterization of mosquitoes (Diptera: Culicidae) from the Colombian rainforest
dc.creator.fl_str_mv Muñoz Gamba, Andrew S.
Laiton Donato, Katherine
Perdomo Balaguera, Erick
Castro, Lyda R.
Usme Ciro, José Aldemar
Parra Henao, Gabriel
dc.contributor.author.none.fl_str_mv Muñoz Gamba, Andrew S.
Laiton Donato, Katherine
Perdomo Balaguera, Erick
Castro, Lyda R.
Usme Ciro, José Aldemar
Parra Henao, Gabriel
dc.subject.spa.fl_str_mv Culicidae
Codigo de barras de ADN
COI
Taxonomia
topic Culicidae
Codigo de barras de ADN
COI
Taxonomia
Culicidae
DNA barcoding
COI
Taxonomy
dc.subject.other.spa.fl_str_mv Culicidae
DNA barcoding
COI
Taxonomy
description A few studies have carried out the taxonomic and molecular characterization of sylvatic mosquito species in Latin America, where some species have been incriminated as vectors for arboviruses and parasites transmission. The present study reports the molecular characterization of mosquito species in the Sierra Nevada de Santa Marta, a natural ecosystem in the Northern coast of Colombia. Manual capture methods were used to collect mosquitoes, and the specimens were identified via classical taxonomy. The COI marker was used for species confirmation, and phylogenetic analysis was performed using the neighbor-joining method, with the Kimura-2-Parameters model. Aedes serratus , Psorophora ferox , Johnbelkinia ulopus , Sabethes chloropterus , Sabethes cyaneus , Wyeomyia aporonoma , Wyeomyia pseudopecten , Wyeomyia ulocoma and Wyeomyia luteoventralis were identified. We assessed the genetic variability of mosquitoes in this area and phylogenetic reconstructions allowed the identification at the species level. Classical and molecular taxonomy demonstrated to be useful and complementary when morphological characteristics are not well preserved, or the taxonomic group is not represented in public molecular databases.
publishDate 2021
dc.date.issued.none.fl_str_mv 2021-03-24
dc.date.accessioned.none.fl_str_mv 2022-08-19T23:43:35Z
dc.date.available.none.fl_str_mv 2022-08-19T23:43:35Z
dc.type.none.fl_str_mv Artículos Científicos
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dc.identifier.bibliographicCitation.spa.fl_str_mv Muñoz-Gamba AS, Laiton-Donato K, Perdomo-Balaguera E, Castro LR, Usme-Ciro JA, Parra-Henao G. Molecular characterization of mosquitoes (Diptera: Culicidae) from the Colombian rainforest. Rev Inst Med Trop Sao Paulo. 2021 Mar 24;63:e24. doi: 10.1590/S1678-9946202163024.https://repository.ucc.edu.co/handle/20.500.12494/46182
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10.1590/S1678-9946202163024
Muñoz-Gamba AS, Laiton-Donato K, Perdomo-Balaguera E, Castro LR, Usme-Ciro JA, Parra-Henao G. Molecular characterization of mosquitoes (Diptera: Culicidae) from the Colombian rainforest. Rev Inst Med Trop Sao Paulo. 2021 Mar 24;63:e24. doi: 10.1590/S1678-9946202163024.https://repository.ucc.edu.co/handle/20.500.12494/46182
url https://hdl.handle.net/20.500.12494/46182
dc.relation.isversionof.spa.fl_str_mv https://www.scielo.br/j/rimtsp/a/WkdHTQm59wsGJk4DLhLJ7gD/?lang=en
dc.relation.ispartofjournal.spa.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo
dc.relation.references.spa.fl_str_mv Harbach RE. The Culicidae (Diptera): a review of taxonomy, classification and phylogeny. Zootaxa. 2007;16681:591-638.
World Health Organization. Vector-borne diseases: key facts. [cited 2021 Jan 21]. Available from: https://www.who.int/en/ news-room/fact-sheets/detail/vector-borne-diseases
Vasilakis N, Cardosa J, Hanley KA, Holmes EC, Weaver SC. Fever from the forest: prospects for the continued emergence of sylvatic dengue virus and its impact on public health. Nat Rev Microbiol. 2011;9:532-41.
Cova GP, Tallaferro DE, Sutil OE. General principles of entomology. Montevideo: Fundación Venezolana para la Salud y la Educación; 1974.
International Commission on Zoological Nomenclature. International code of zoological nomenclature. 4th ed. London: International Trust for Zoological Nomenclature; 1999. [cited 2021 Jan 21] Available from: https://www.iczn.org/the-code/ the-international-code-of-zoological-nomenclature/the-codeonline/
Kress WJ, Garcia-Robledo C, Uriarte M, Erickson DL. DNA barcodes for ecology, evolution, and conservation. Trends Ecol Evol. 2015;30:25-35.
Shah MA, Khan AA, Khan HZ. DNA barcoding-applications in insect ecology. Indian J Ecol. 2015;42:287-94
Arnot DE, Roper C, Bayoumi RA. Digital codes from hypervariable tandemly repeated DNA sequences in the Plasmodium falciparum circumsporozoite gene can genetically barcode isolates. Mol Biochem Parasitol. 1993;61:15-24.
Hebert PD, Ratnasingham S, deWaard JR. Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species. Proc Biol Sci. 2003;270 Suppl 1:S96-9.
Naranjo-Diaz N, Rosero DA, Rua-Uribe G, Luckhart S, Correa MM. Abundance, behavior and entomological inoculation rates of anthropophilic anophelines from a primary Colombian malaria endemic area. Parasit Vectors. 2013;6:61.
Rozo-Lopez P, Mengual X. Mosquito species (Diptera, Culicidae) in three ecosystems from the Colombian Andes: identification through DNA barcoding and adult morphology. Zookeys. 2015;513:39-64.
Sánchez J, Galeano, Y, Rosero DA, Naranjo NJ, Correa MM. Confirmación molecular de especie en especímenes Anopheles recolectados en el corregimiento de Juan José, Puerto Libertador, Córdoba, Colombia. Hechos Microbiol. 2011;2:19- 28.
Vidales H, Buitrago B, Sanín LH, Morales A, Groot H. Estudio de un brote epidémico de fiebre amarilla selvática en el pie de monte de La Sierra Nevada de Santa Marta, 1979. Biomédica. 1981;1:171-86.
Balaguera L, Cantillo J, Camargo LF, Gómez A, Jiménez M, Laverde GN, et al. Plan de manejo Parque Nacional Natural Sierra Nevada de Santa Marta. Santa Marta: Unidad Administrativa Especial del Sistema de Parques Nacionales Naturales; 2015. [cited 2021 Jan 21]. Available from: https://www.parquesnacionales.gov.co/portal/wp-content/ uploads/2013/12/parqueSierraNevadadeSantaMarta.pdf
Colombia. Banco de la República. Sierra Nevada de Santa Marta: economía de sus recursos naturales. [cited 2021 Jan 21]. Available from: https://www.banrep.gov.co/es/sierra-nevadasanta-marta-economia-sus-recursos-naturales
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dc.publisher.spa.fl_str_mv Universidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Medicina, Santa Marta
Thelma Suely Okay
dc.publisher.program.spa.fl_str_mv Medicina
dc.publisher.place.spa.fl_str_mv Santa Marta
institution Universidad Cooperativa de Colombia
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spelling Muñoz Gamba, Andrew S.Laiton Donato, KatherinePerdomo Balaguera, ErickCastro, Lyda R.Usme Ciro, José AldemarParra Henao, Gabriel632022-08-19T23:43:35Z2022-08-19T23:43:35Z2021-03-241678-994610.1590/S1678-9946202163024https://hdl.handle.net/20.500.12494/46182Muñoz-Gamba AS, Laiton-Donato K, Perdomo-Balaguera E, Castro LR, Usme-Ciro JA, Parra-Henao G. Molecular characterization of mosquitoes (Diptera: Culicidae) from the Colombian rainforest. Rev Inst Med Trop Sao Paulo. 2021 Mar 24;63:e24. doi: 10.1590/S1678-9946202163024.https://repository.ucc.edu.co/handle/20.500.12494/46182A few studies have carried out the taxonomic and molecular characterization of sylvatic mosquito species in Latin America, where some species have been incriminated as vectors for arboviruses and parasites transmission. The present study reports the molecular characterization of mosquito species in the Sierra Nevada de Santa Marta, a natural ecosystem in the Northern coast of Colombia. Manual capture methods were used to collect mosquitoes, and the specimens were identified via classical taxonomy. The COI marker was used for species confirmation, and phylogenetic analysis was performed using the neighbor-joining method, with the Kimura-2-Parameters model. Aedes serratus , Psorophora ferox , Johnbelkinia ulopus , Sabethes chloropterus , Sabethes cyaneus , Wyeomyia aporonoma , Wyeomyia pseudopecten , Wyeomyia ulocoma and Wyeomyia luteoventralis were identified. We assessed the genetic variability of mosquitoes in this area and phylogenetic reconstructions allowed the identification at the species level. Classical and molecular taxonomy demonstrated to be useful and complementary when morphological characteristics are not well preserved, or the taxonomic group is not represented in public molecular databases.https://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0000318507https://orcid.org/0000-0002-8093-0544https://scienti.minciencias.gov.co/gruplac/jsp/visualiza/visualizagr.jsp?nro=00000000008981jose.usmec@campusucc.edu.cohttps://scholar.google.com.co/citations?user=cU2KyT4AAAAJ&hl=en11Universidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Medicina, Santa MartaThelma Suely OkayMedicinaSanta Martahttps://www.scielo.br/j/rimtsp/a/WkdHTQm59wsGJk4DLhLJ7gD/?lang=enRevista do Instituto de Medicina Tropical de São PauloHarbach RE. The Culicidae (Diptera): a review of taxonomy, classification and phylogeny. Zootaxa. 2007;16681:591-638.World Health Organization. Vector-borne diseases: key facts. [cited 2021 Jan 21]. Available from: https://www.who.int/en/ news-room/fact-sheets/detail/vector-borne-diseasesVasilakis N, Cardosa J, Hanley KA, Holmes EC, Weaver SC. Fever from the forest: prospects for the continued emergence of sylvatic dengue virus and its impact on public health. Nat Rev Microbiol. 2011;9:532-41.Cova GP, Tallaferro DE, Sutil OE. General principles of entomology. Montevideo: Fundación Venezolana para la Salud y la Educación; 1974.International Commission on Zoological Nomenclature. International code of zoological nomenclature. 4th ed. London: International Trust for Zoological Nomenclature; 1999. [cited 2021 Jan 21] Available from: https://www.iczn.org/the-code/ the-international-code-of-zoological-nomenclature/the-codeonline/Kress WJ, Garcia-Robledo C, Uriarte M, Erickson DL. DNA barcodes for ecology, evolution, and conservation. Trends Ecol Evol. 2015;30:25-35.Shah MA, Khan AA, Khan HZ. DNA barcoding-applications in insect ecology. Indian J Ecol. 2015;42:287-94Arnot DE, Roper C, Bayoumi RA. Digital codes from hypervariable tandemly repeated DNA sequences in the Plasmodium falciparum circumsporozoite gene can genetically barcode isolates. Mol Biochem Parasitol. 1993;61:15-24.Hebert PD, Ratnasingham S, deWaard JR. Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species. Proc Biol Sci. 2003;270 Suppl 1:S96-9.Naranjo-Diaz N, Rosero DA, Rua-Uribe G, Luckhart S, Correa MM. Abundance, behavior and entomological inoculation rates of anthropophilic anophelines from a primary Colombian malaria endemic area. Parasit Vectors. 2013;6:61.Rozo-Lopez P, Mengual X. Mosquito species (Diptera, Culicidae) in three ecosystems from the Colombian Andes: identification through DNA barcoding and adult morphology. Zookeys. 2015;513:39-64.Sánchez J, Galeano, Y, Rosero DA, Naranjo NJ, Correa MM. Confirmación molecular de especie en especímenes Anopheles recolectados en el corregimiento de Juan José, Puerto Libertador, Córdoba, Colombia. Hechos Microbiol. 2011;2:19- 28.Vidales H, Buitrago B, Sanín LH, Morales A, Groot H. Estudio de un brote epidémico de fiebre amarilla selvática en el pie de monte de La Sierra Nevada de Santa Marta, 1979. Biomédica. 1981;1:171-86.Balaguera L, Cantillo J, Camargo LF, Gómez A, Jiménez M, Laverde GN, et al. Plan de manejo Parque Nacional Natural Sierra Nevada de Santa Marta. Santa Marta: Unidad Administrativa Especial del Sistema de Parques Nacionales Naturales; 2015. [cited 2021 Jan 21]. Available from: https://www.parquesnacionales.gov.co/portal/wp-content/ uploads/2013/12/parqueSierraNevadadeSantaMarta.pdfColombia. Banco de la República. Sierra Nevada de Santa Marta: economía de sus recursos naturales. [cited 2021 Jan 21]. Available from: https://www.banrep.gov.co/es/sierra-nevadasanta-marta-economia-sus-recursos-naturalesCulicidaeCodigo de barras de ADNCOITaxonomiaCulicidaeDNA barcodingCOITaxonomyMolecular characterization of mosquitoes (Diptera: Culicidae) from the Colombian rainforestArtículos Científicoshttp://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAtribucióninfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2PublicationLICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repository.ucc.edu.co/bitstreams/3dc92251-6416-4bc9-9558-3f1ad634e952/download8a4605be74aa9ea9d79846c1fba20a33MD54ORIGINAL32. Molecular characterization of mosquitoes (Diptera Culicidae) from the Colombian rainforest. Munoz-Gamba et al 2021.pdf32. Molecular characterization of mosquitoes (Diptera Culicidae) from the Colombian rainforest. 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