Generation of a DNA-Launched reporter replicon based on dengue virus type 2 as a multipurpose platform
Artículo científico publicado en revista indexada en Scopus y categorizada en Q2.
- Autores:
-
Usme Ciro, José Aldemar
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2016
- Institución:
- Universidad Cooperativa de Colombia
- Repositorio:
- Repositorio UCC
- Idioma:
- OAI Identifier:
- oai:repository.ucc.edu.co:20.500.12494/978
- Acceso en línea:
- https://hdl.handle.net/20.500.12494/978
- Palabra clave:
- Virus dengue
Cromosoma artificial bacteriano
Replicon
Flavivirus
- Rights
- openAccess
- License
- Licencia CC
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Generation of a DNA-Launched reporter replicon based on dengue virus type 2 as a multipurpose platform |
| title |
Generation of a DNA-Launched reporter replicon based on dengue virus type 2 as a multipurpose platform |
| spellingShingle |
Generation of a DNA-Launched reporter replicon based on dengue virus type 2 as a multipurpose platform Virus dengue Cromosoma artificial bacteriano Replicon Flavivirus |
| title_short |
Generation of a DNA-Launched reporter replicon based on dengue virus type 2 as a multipurpose platform |
| title_full |
Generation of a DNA-Launched reporter replicon based on dengue virus type 2 as a multipurpose platform |
| title_fullStr |
Generation of a DNA-Launched reporter replicon based on dengue virus type 2 as a multipurpose platform |
| title_full_unstemmed |
Generation of a DNA-Launched reporter replicon based on dengue virus type 2 as a multipurpose platform |
| title_sort |
Generation of a DNA-Launched reporter replicon based on dengue virus type 2 as a multipurpose platform |
| dc.creator.fl_str_mv |
Usme Ciro, José Aldemar |
| dc.contributor.author.none.fl_str_mv |
Usme Ciro, José Aldemar |
| dc.subject.spa.fl_str_mv |
Virus dengue Cromosoma artificial bacteriano Replicon Flavivirus |
| topic |
Virus dengue Cromosoma artificial bacteriano Replicon Flavivirus |
| description |
Artículo científico publicado en revista indexada en Scopus y categorizada en Q2. |
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2016 |
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2016-06-23 |
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2017-08-09T01:14:23Z |
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Usme Ciro, J. A., Lopera, J. A., Alvarez, D. A., Enjuanes, L., & Almazán, F. (2016). Generation of a DNA-Launched Reporter Replicon Based on Dengue Virus Type 2 as a Multipurpose Platform. Intervirology, 59(5-6), 275-282. doi:10.1159/000476066 |
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Usme Ciro, J. A., Lopera, J. A., Alvarez, D. A., Enjuanes, L., & Almazán, F. (2016). Generation of a DNA-Launched Reporter Replicon Based on Dengue Virus Type 2 as a Multipurpose Platform. Intervirology, 59(5-6), 275-282. doi:10.1159/000476066 |
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Licencia CC |
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openAccess |
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Universidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Programa de Medicina, Santa Marta, Colombia, 00000 |
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Medicina |
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Santa Marta |
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Universidad Cooperativa de Colombia |
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Usme Ciro, José Aldemar2017-08-09T01:14:23Z2017-08-09T01:14:23Z2016-06-23https://hdl.handle.net/20.500.12494/978Usme Ciro, J. A., Lopera, J. A., Alvarez, D. A., Enjuanes, L., & Almazán, F. (2016). Generation of a DNA-Launched Reporter Replicon Based on Dengue Virus Type 2 as a Multipurpose Platform. Intervirology, 59(5-6), 275-282. doi:10.1159/000476066Artículo científico publicado en revista indexada en Scopus y categorizada en Q2.Dengue viruses (DENV) have become the most important arthropod-borne viruses, causing dengue and severe dengue fever in at least 50-100 million cases each year, mainly in tropical and subtropical countries. During recent years, important advances in the molecular biology concerning the life cycle of these viruses have allowed the manipulation and generation of recombinant viruses and replicons with multiple applications, mainly in viral biology and the screening of antiviral compounds. In the present study, we describe the construction of an enhanced green fluorescent protein-bearing DENV replicon under the control of the cytomegalovirus immediate early promoter. Following a rational in silico design and cloning by standard molecular biology techniques, a reporter DENV-2 replicon and a replication-deficient mutant were constructed, and characterized by confocal microscopy and real-time RT-PCR. The results showed successful transcription, translation, and autonomous viral RNA replication of the DENV replicon from its DNA clone. This novel DENV replicon will allow the study of viral replication and testing of antiviral candidates without the need for in vitro transcription.jose.usmec@campusucc.edu.coUniversidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Programa de Medicina, Santa Marta, Colombia, 00000MedicinaSanta Martahttps://www.karger.com/Article/FullText/476066Virus dengueCromosoma artificial bacterianoRepliconFlavivirusGeneration of a DNA-Launched reporter replicon based on dengue virus type 2 as a multipurpose platformArtículohttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionLicencia CCinfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2PublicationORIGINAL18. Generation of a DNA-launched reporter replicon based on DENV type 2 as a multipurpose platform. Usme-Ciro et al 2016.pdf18. Generation of a DNA-launched reporter replicon based on DENV type 2 as a multipurpose platform. Usme-Ciro et al 2016.pdfapplication/pdf431134https://repository.ucc.edu.co/bitstreams/72697b8b-8c3a-43b9-a032-d074ccc87fac/download82b6ac173910e560f85ced46e5abc73eMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repository.ucc.edu.co/bitstreams/daa3f1ef-cdb5-44c1-86de-c94cec08c1ec/download8a4605be74aa9ea9d79846c1fba20a33MD52TEXT18. Generation of a DNA-launched reporter replicon based on DENV type 2 as a multipurpose platform. Usme-Ciro et al 2016.pdf.txt18. Generation of a DNA-launched reporter replicon based on DENV type 2 as a multipurpose platform. Usme-Ciro et al 2016.pdf.txtExtracted texttext/plain32275https://repository.ucc.edu.co/bitstreams/19baa0e2-5cd6-4986-a264-0321875ad989/downloadebfc3a03d617766e9f30a612cc8dd1a4MD53THUMBNAIL18. Generation of a DNA-launched reporter replicon based on DENV type 2 as a multipurpose platform. Usme-Ciro et al 2016.pdf.jpg18. 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