Calcitriol decreases HIV-1 transfer in vitro from monocyte-derived dendritic cells to CD4 + T cells, and downregulates the expression of DC-SIGN and SIGLEC-1

Dendritic cells (DCs) promote HIV-1 transmission by acting as Trojan horses, capturing viral particles, facilitating the infection of CD4+ T-cells. Vitamin D (VitD) has shown to decrease T cell activation, reducing susceptibility to HIV-1 infection of CD4+ T-cells in vitro; however, if VitD decrease...

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Autores:: Álvarez, Natalia
Gonzalez, Sandra M
Hernández López, Juan Carlos
Rugeles, Maria T
Aguilar-Jimenez, Wbeimar

Tipo de recurso:: Article of investigation

Fecha de publicación:: 2021

Institución:: Universidad Cooperativa de Colombia

Repositorio:: Repositorio UCC

Idioma:

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network_acronym_str	COOPER2
network_name_str	Repositorio UCC
repository_id_str
dc.title.spa.fl_str_mv	Calcitriol decreases HIV-1 transfer in vitro from monocyte-derived dendritic cells to CD4 + T cells, and downregulates the expression of DC-SIGN and SIGLEC-1
title	Calcitriol decreases HIV-1 transfer in vitro from monocyte-derived dendritic cells to CD4 + T cells, and downregulates the expression of DC-SIGN and SIGLEC-1
spellingShingle	Calcitriol decreases HIV-1 transfer in vitro from monocyte-derived dendritic cells to CD4 + T cells, and downregulates the expression of DC-SIGN and SIGLEC-1 Calcitriol HIV-1 monocyte-derived dendritic cells CD4 + T cells DC-SIGN SIGLEC-1
title_short	Calcitriol decreases HIV-1 transfer in vitro from monocyte-derived dendritic cells to CD4 + T cells, and downregulates the expression of DC-SIGN and SIGLEC-1
title_full	Calcitriol decreases HIV-1 transfer in vitro from monocyte-derived dendritic cells to CD4 + T cells, and downregulates the expression of DC-SIGN and SIGLEC-1
title_fullStr	Calcitriol decreases HIV-1 transfer in vitro from monocyte-derived dendritic cells to CD4 + T cells, and downregulates the expression of DC-SIGN and SIGLEC-1
title_full_unstemmed	Calcitriol decreases HIV-1 transfer in vitro from monocyte-derived dendritic cells to CD4 + T cells, and downregulates the expression of DC-SIGN and SIGLEC-1
title_sort	Calcitriol decreases HIV-1 transfer in vitro from monocyte-derived dendritic cells to CD4 + T cells, and downregulates the expression of DC-SIGN and SIGLEC-1
dc.creator.fl_str_mv	Álvarez, Natalia Gonzalez, Sandra M Hernández López, Juan Carlos Rugeles, Maria T Aguilar-Jimenez, Wbeimar
dc.contributor.author.none.fl_str_mv	Álvarez, Natalia Gonzalez, Sandra M Hernández López, Juan Carlos Rugeles, Maria T Aguilar-Jimenez, Wbeimar
dc.subject.spa.fl_str_mv	Calcitriol HIV-1 monocyte-derived dendritic cells CD4 + T cells DC-SIGN SIGLEC-1
topic	Calcitriol HIV-1 monocyte-derived dendritic cells CD4 + T cells DC-SIGN SIGLEC-1
description	Dendritic cells (DCs) promote HIV-1 transmission by acting as Trojan horses, capturing viral particles, facilitating the infection of CD4+ T-cells. Vitamin D (VitD) has shown to decrease T cell activation, reducing susceptibility to HIV-1 infection of CD4+ T-cells in vitro; however, if VitD decreases viral transfer from DCs to CD4+ T-cells is unknown. In this study, we co-cultured HIV-1-pulsed immature and LPS mature monocytes-derived DCs (iDCs and LmDCs, respectively), differentiated in presence or absence of calcitriol (VitD active form), with PHAactivated autologous CD4+ T-cells from 16 healthy donors. In co-cultures of iDCs and LmDCs treated with calcitriol, there was a significant decrease in frequency of infected CD4 + T-cells, evaluated by flow cytometry. However, p24 levels evaluated by ELISA were not significantly reduced in culture supernatants. Moreover, calcitriol-treated iDCs exhibited decreased expression of genes involved in HIV-1 transfer compared to the control. Both, calcitriol-treated iDCs and LmDCs exhibit a similar gene expression profile, probably related to a transcriptional balance achieved after long treatment with calcitriol. Since calcitriol-differentiated DCs express on their surface a lower amount of DC-SIGN and SIGLEC-1 molecules, widely associated with HIV-1 transfer, suggesting that this mechanism contributes to a lower transfer of viral particles by the DCs.
publishDate	2021
dc.date.issued.none.fl_str_mv	2021-09-09
dc.date.accessioned.none.fl_str_mv	2022-08-16T14:45:05Z
dc.date.available.none.fl_str_mv	2022-08-16T14:45:05Z
dc.type.none.fl_str_mv	Artículos Científicos
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dc.type.driver.none.fl_str_mv	info:eu-repo/semantics/article
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dc.identifier.uri.spa.fl_str_mv	https://doi. org/10.1371/journal.pone.0269932
dc.identifier.uri.none.fl_str_mv	https://hdl.handle.net/20.500.12494/46142
dc.identifier.bibliographicCitation.spa.fl_str_mv	: Alvarez N, Gonzalez SM, Hernandez JC, Rugeles MT, Aguilar-Jimenez W (2022) Calcitriol decreases HIV-1 transfer in vitro from monocytederived dendritic cells to CD4 + T cells, and downregulates the expression of DC-SIGN and SIGLEC-1. PLoS ONE 17(7): e0269932. https://doi. org/10.1371/journal.pone.0269932
url	https://doi. org/10.1371/journal.pone.0269932 https://hdl.handle.net/20.500.12494/46142
identifier_str_mv	: Alvarez N, Gonzalez SM, Hernandez JC, Rugeles MT, Aguilar-Jimenez W (2022) Calcitriol decreases HIV-1 transfer in vitro from monocytederived dendritic cells to CD4 + T cells, and downregulates the expression of DC-SIGN and SIGLEC-1. PLoS ONE 17(7): e0269932. https://doi. org/10.1371/journal.pone.0269932
dc.relation.ispartofjournal.spa.fl_str_mv	Plos one
dc.relation.references.spa.fl_str_mv	1. UNAIDS. 2019 GLOBAL HIV STATISTICS. (2020) 2. Calton EK, Keane KN, Newsholme P, Soares MJ. The Impact of Vitamin D Levels on Inflammatory Status: A Systematic Review of Immune Cell Studies. PLoS One (2015) 10(11):1–12. 3. Coussens AK, Martineau AR, Wilkinson RJ. Anti-Inflammatory and Antimicrobial Actions of Vitamin D in Combating TB/HIV. Scientifica (Cairo) (2014) 2014:1–13 4. Beard JA, Bearden A, Striker R. Vitamin D and the anti-viral state. J Clin Virol (2011) 50:194–200. 5. Prietl B, Treiber G, Pieber TR, Amrein K. Vitamin D and immune function. Nutrients (2013) 5:2502– 2521. 6. Arboleda Alzate JF, Rodenhuis-Zybert IA, Herna´ndez JC, Smit JM, Urcuqui-Inchima S. Human macrophages differentiated in the presence of vitamin D3restrict dengue virus infection and innate responses by downregulating mannose receptor expression. PLoS Negl Trop Dis (2017) 11:1–18. 7. Aguilar-Jime´nez W, Zapata W, Caruz A, Rugeles MT. High transcript levels of vitamin D receptor are correlated with higher mRNA expression of human beta defensins and IL-10 in Mucosa of HIV-1- exposed seronegative individuals. PLoS One (2013) 8: 8. Coussens AK, Naude CE, Goliath R, Chaplin G, Wilkinson RJ, Jablonski NG. High-dose vitamin D 3 reduces deficiency caused by low UVB exposure and limits HIV-1 replication in urban Southern Africans. Proc Natl Acad Sci (2015) 112:8052–8057 9. Aguilar-Jimenez W, Villegas-Ospina S, Gonzalez S, Zapata W, Saulle I, Garziano M, et al. Precursor forms of Vitamin D reduce HIV-1 infection in vitro. in Journal of Acquired Immune Deficiency Syndromes, 497–506. 10. Gonzalez SM, Aguilar-Jimenez W, Trujillo-Gil E, Zapata W, Su R-C, Ball TB, et al. Vitamin D treatment of peripheral blood mononuclear cells modulated immune activation and reduced susceptibility to HIV-1 infection of CD4+ T lymphocytes. PLoS One (2019) 14:e0222878 11. Barragan M, Good M, Kolls JK. Regulation of dendritic cell function by vitamin D. Nutrients (2015) 7:8127–8151 12. Berer A, Sto¨ckl J, Majdic O, Wagner T, Kollars M, Lechner K, et al. 1,25-Dihydroxyvitamin D 3 inhibits dendritic cell differentiation and maturation in vitro. Elsevier (2000) 28:575–583 13. Penna G, Adorini L. 1 α, 25-Dihydroxyvitamin D 3 Inhibits Differentiation, Maturation, Activation, and Survival of Dendritic Cells Leading to Impaired Alloreactive T Cell Activation. J Immunol (2014) 164(5): 14. Wang J, Janas AM, Olson WJ, Wu L. Functionally Distinct Transmission of Human Immunodeficiency Virus Type 1 Mediated by Immature and Mature Dendritic Cells. J Virol (2007) 81:8933–8943 15. Cameron PU, Freudenthal PS, Barker JM, Gezelter S, Inaba K, Steinman RM. Dendritic cells exposed to human immunodeficiency virus type-1 transmit a vigorous cytopathic infection to CD4+ T cells. Science (80-) (1992) 257:383–387
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dc.coverage.temporal.spa.fl_str_mv	17(7)
dc.publisher.spa.fl_str_mv	Universidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Programa de Medicina, Medellín y Envigado, Colombia, 00000
dc.publisher.program.spa.fl_str_mv	Medicina
dc.publisher.place.spa.fl_str_mv	Medellín
institution	Universidad Cooperativa de Colombia
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spelling	Álvarez, NataliaGonzalez, Sandra MHernández López, Juan Carlos Rugeles, Maria TAguilar-Jimenez, Wbeimar17(7)2022-08-16T14:45:05Z2022-08-16T14:45:05Z2021-09-09https://doi. org/10.1371/journal.pone.0269932https://hdl.handle.net/20.500.12494/46142: Alvarez N, Gonzalez SM, Hernandez JC, Rugeles MT, Aguilar-Jimenez W (2022) Calcitriol decreases HIV-1 transfer in vitro from monocytederived dendritic cells to CD4 + T cells, and downregulates the expression of DC-SIGN and SIGLEC-1. PLoS ONE 17(7): e0269932. https://doi. org/10.1371/journal.pone.0269932Dendritic cells (DCs) promote HIV-1 transmission by acting as Trojan horses, capturing viral particles, facilitating the infection of CD4+ T-cells. Vitamin D (VitD) has shown to decrease T cell activation, reducing susceptibility to HIV-1 infection of CD4+ T-cells in vitro; however, if VitD decreases viral transfer from DCs to CD4+ T-cells is unknown. In this study, we co-cultured HIV-1-pulsed immature and LPS mature monocytes-derived DCs (iDCs and LmDCs, respectively), differentiated in presence or absence of calcitriol (VitD active form), with PHAactivated autologous CD4+ T-cells from 16 healthy donors. In co-cultures of iDCs and LmDCs treated with calcitriol, there was a significant decrease in frequency of infected CD4 + T-cells, evaluated by flow cytometry. However, p24 levels evaluated by ELISA were not significantly reduced in culture supernatants. Moreover, calcitriol-treated iDCs exhibited decreased expression of genes involved in HIV-1 transfer compared to the control. Both, calcitriol-treated iDCs and LmDCs exhibit a similar gene expression profile, probably related to a transcriptional balance achieved after long treatment with calcitriol. Since calcitriol-differentiated DCs express on their surface a lower amount of DC-SIGN and SIGLEC-1 molecules, widely associated with HIV-1 transfer, suggesting that this mechanism contributes to a lower transfer of viral particles by the DCs. https://scienti.colciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0000283088http://orcid.org/0000-0002-9200-5698https://scienti.colciencias.gov.co/gruplac/jsp/visualiza/visualizagr.jsp?nro=00000000011355juanc.hernandezl@campusucc.edu.co1-14Universidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Programa de Medicina, Medellín y Envigado, Colombia, 00000MedicinaMedellínCalcitriolHIV-1monocyte-derived dendritic cellsCD4 + T cellsDC-SIGNSIGLEC-1Calcitriol decreases HIV-1 transfer in vitro from monocyte-derived dendritic cells to CD4 + T cells, and downregulates the expression of DC-SIGN and SIGLEC-1Artículos Científicoshttp://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionAtribucióninfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Plos one1. UNAIDS. 2019 GLOBAL HIV STATISTICS. (2020)2. Calton EK, Keane KN, Newsholme P, Soares MJ. The Impact of Vitamin D Levels on Inflammatory Status: A Systematic Review of Immune Cell Studies. PLoS One (2015) 10(11):1–12.3. Coussens AK, Martineau AR, Wilkinson RJ. Anti-Inflammatory and Antimicrobial Actions of Vitamin D in Combating TB/HIV. Scientifica (Cairo) (2014) 2014:1–134. Beard JA, Bearden A, Striker R. Vitamin D and the anti-viral state. J Clin Virol (2011) 50:194–200.5. Prietl B, Treiber G, Pieber TR, Amrein K. Vitamin D and immune function. Nutrients (2013) 5:2502– 2521.6. Arboleda Alzate JF, Rodenhuis-Zybert IA, Herna´ndez JC, Smit JM, Urcuqui-Inchima S. Human macrophages differentiated in the presence of vitamin D3restrict dengue virus infection and innate responses by downregulating mannose receptor expression. PLoS Negl Trop Dis (2017) 11:1–18.7. Aguilar-Jime´nez W, Zapata W, Caruz A, Rugeles MT. High transcript levels of vitamin D receptor are correlated with higher mRNA expression of human beta defensins and IL-10 in Mucosa of HIV-1- exposed seronegative individuals. PLoS One (2013) 8:8. Coussens AK, Naude CE, Goliath R, Chaplin G, Wilkinson RJ, Jablonski NG. High-dose vitamin D 3 reduces deficiency caused by low UVB exposure and limits HIV-1 replication in urban Southern Africans. Proc Natl Acad Sci (2015) 112:8052–80579. Aguilar-Jimenez W, Villegas-Ospina S, Gonzalez S, Zapata W, Saulle I, Garziano M, et al. Precursor forms of Vitamin D reduce HIV-1 infection in vitro. in Journal of Acquired Immune Deficiency Syndromes, 497–506.10. Gonzalez SM, Aguilar-Jimenez W, Trujillo-Gil E, Zapata W, Su R-C, Ball TB, et al. Vitamin D treatment of peripheral blood mononuclear cells modulated immune activation and reduced susceptibility to HIV-1 infection of CD4+ T lymphocytes. PLoS One (2019) 14:e022287811. Barragan M, Good M, Kolls JK. Regulation of dendritic cell function by vitamin D. Nutrients (2015) 7:8127–815112. Berer A, Sto¨ckl J, Majdic O, Wagner T, Kollars M, Lechner K, et al. 1,25-Dihydroxyvitamin D 3 inhibits dendritic cell differentiation and maturation in vitro. Elsevier (2000) 28:575–58313. Penna G, Adorini L. 1 α, 25-Dihydroxyvitamin D 3 Inhibits Differentiation, Maturation, Activation, and Survival of Dendritic Cells Leading to Impaired Alloreactive T Cell Activation. J Immunol (2014) 164(5):14. Wang J, Janas AM, Olson WJ, Wu L. Functionally Distinct Transmission of Human Immunodeficiency Virus Type 1 Mediated by Immature and Mature Dendritic Cells. J Virol (2007) 81:8933–894315. Cameron PU, Freudenthal PS, Barker JM, Gezelter S, Inaba K, Steinman RM. Dendritic cells exposed to human immunodeficiency virus type-1 transmit a vigorous cytopathic infection to CD4+ T cells. 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Calcitriol decreases HIV-1 transfer in vitro from monocyte-derived dendritic cells to CD4 + T cells, and downregulates the expression of DC-SIGN and SIGLEC-1

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