Inyección intracitoplasmática del espermatozoide en la reproducción asistida en bovinos: revisión literaria.

La inyección intracitoplasmática del espermatozoide (ICSI) es una técnica reciente de la reproducción asistida que se ha prescrito en la mayoría de los mamíferos con infertilidad en el macho pero que ha tenido bastante acogida y evolución desde su descubrimiento. Aunque en humanos se ha logrado sobr...

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Autores:: Casas Silva, Santiago E.

Tipo de recurso:: Trabajo de grado de pregrado

Fecha de publicación:: 2018

Institución:: Universidad Cooperativa de Colombia

Repositorio:: Repositorio UCC

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repository_id_str
dc.title.spa.fl_str_mv	Inyección intracitoplasmática del espermatozoide en la reproducción asistida en bovinos: revisión literaria.
title	Inyección intracitoplasmática del espermatozoide en la reproducción asistida en bovinos: revisión literaria.
spellingShingle	Inyección intracitoplasmática del espermatozoide en la reproducción asistida en bovinos: revisión literaria. ICSI Semen sexado Reproducción bovina Embriones Ovocito Infertilidad TG 2018 MVZ 8133 ICSI Sexed semen Bovine reproduction Embryos Oocyte Infertility
title_short	Inyección intracitoplasmática del espermatozoide en la reproducción asistida en bovinos: revisión literaria.
title_full	Inyección intracitoplasmática del espermatozoide en la reproducción asistida en bovinos: revisión literaria.
title_fullStr	Inyección intracitoplasmática del espermatozoide en la reproducción asistida en bovinos: revisión literaria.
title_full_unstemmed	Inyección intracitoplasmática del espermatozoide en la reproducción asistida en bovinos: revisión literaria.
title_sort	Inyección intracitoplasmática del espermatozoide en la reproducción asistida en bovinos: revisión literaria.
dc.creator.fl_str_mv	Casas Silva, Santiago E.
dc.contributor.advisor.none.fl_str_mv	Trujillo Piso, Dunia Yisela
dc.contributor.author.none.fl_str_mv	Casas Silva, Santiago E.
dc.subject.spa.fl_str_mv	ICSI Semen sexado Reproducción bovina Embriones Ovocito Infertilidad
topic	ICSI Semen sexado Reproducción bovina Embriones Ovocito Infertilidad TG 2018 MVZ 8133 ICSI Sexed semen Bovine reproduction Embryos Oocyte Infertility
dc.subject.classification.spa.fl_str_mv	TG 2018 MVZ 8133
dc.subject.other.spa.fl_str_mv	ICSI Sexed semen Bovine reproduction Embryos Oocyte Infertility
description	La inyección intracitoplasmática del espermatozoide (ICSI) es una técnica reciente de la reproducción asistida que se ha prescrito en la mayoría de los mamíferos con infertilidad en el macho pero que ha tenido bastante acogida y evolución desde su descubrimiento. Aunque en humanos se ha logrado sobrepasar varias barreras, en bovinos se han vivido ciertas dificultades relacionadas con el desarrollo embrionario y fetal debido a las características de sus gametos y al desarrollo de la técnica. Es un desafío para el Médico veterinario realizar estos procedimientos en campo, pero debido a su eficacia, se ha logrado continuar con la técnica estudiando sus mejoras y realizando acciones que lleven a optimizar el proceso desde la recolección de ovocitos hasta su transferencia embrionaria y posterior preñez. El objetivo de esta revisión es la actualización de la información más reciente al respecto de la técnica ICSI, sus ventajas y desventajas en las producciones ganaderas, para convertirse en una herramienta literaria para los médicos veterinarios dedicados a la reproducción bovina.
publishDate	2018
dc.date.issued.none.fl_str_mv	2018-06
dc.date.accessioned.none.fl_str_mv	2019-04-03T13:43:41Z
dc.date.available.none.fl_str_mv	2019-04-03T13:43:41Z
dc.type.none.fl_str_mv	Trabajo de grado - Pregrado
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dc.identifier.uri.none.fl_str_mv	https://hdl.handle.net/20.500.12494/8133
dc.identifier.bibliographicCitation.spa.fl_str_mv	Casas Silva. S. E. (2018). Inyección intracitoplasmática del espermatozoide en la reproducción asistida en bovinos: revisión literaria. (Tesis de pregrado). Recuperado de: http://repository.ucc.edu.co/handle/ucc/8133
url	https://hdl.handle.net/20.500.12494/8133
identifier_str_mv	Casas Silva. S. E. (2018). Inyección intracitoplasmática del espermatozoide en la reproducción asistida en bovinos: revisión literaria. (Tesis de pregrado). Recuperado de: http://repository.ucc.edu.co/handle/ucc/8133
dc.relation.references.spa.fl_str_mv	Abdalla H, Shimoda M, Hirabayashi M, Hochi S. 2009. A combined treatment of ionomycin with ethanol improves blastocyst development of bovine oocytes harvested from stored ovaries and microinjected with spermatozoa. Theriogenology, 72 453-460. Abdalla, H., Shimoda, M., Hara, H., Morita, H., Kuwayama, M., Hirabayashi, M., & Hochi, S. (2010). Vitrification of ICSI- and IVF-derived bovine blastocysts by minimum volume cooling procedure: effect of developmental stage and age. Theriogenology, 74(6), 1028-1035. Doi: 10.1016/j.theriogenology.2010.04.033 Águila, L., Zambrano, F., Arias, M., & Felmer, R. (2017). Sperm capacitation pretreatment positively impacts bovine intracytoplasmic sperm injection. Molecular Reproduction And Development, 84(7), 649-659. Doi: 10.1002/mrd.22834 Ajduk, A., Yamauchi, Y., & Ward, M. (2006). Sperm Chromatin Remodeling after Intracytoplasmic Sperm Injection Differs from That of In Vitro Fertilization1. Biology Of Reproduction, 75(3), 442-451. Doi: 10.1095/biolreprod.106.053223 Arias Cea, M. (2013). Optimizacion de la Técnica de Inyección Intracitoplasmatica de Espermatozoides (ICSI) en Bovinos y Evaluación de la Calidad de los Embriones Generados (Doctorado). Universidad de la Frontera Facultad de Ciencias Agropecuarias y Forestales. Arias, M., Risopatrón, J., Sánchez, R., & Felmer, R. (2015). Intracytoplasmic sperm injection affects embryo developmental potential and gene expression in cattle. Reproductive Biology, 15(1), 34-41. Doi: 10.1016/j.repbio.2014.11.001 Assumpção, M.; Haipeck, k.; Lima, A.S.; Mello, M.; Oliveira, l.; Oliveira, V.;Tavares, l.; Visintin, J. Capacitação espermática In Vitro com heparina e cálcio ionóforo e sua correlação com a fertilidade em touros. Braz. J. Vet. Res. Anim. Sci 39, Numero 2: 149-156. 2002. Benavides Torres, R. (2008). Evaluación de Tres Protocolos de Capacitación Espermática Bovina In Vitro a Través de la Penetración Oocitaria (Pregrado). Universidad de la Salle Facultad de Medicina Veterinaria Bevacqua, R., Pereyra-Bonnet, F., Fernandez-Martin, R., & Salamone, D. (2010). High rates of bovine blastocyst development after ICSI-mediated gene transfer assisted by chemical activation. Theriogenology, 74(6), 922-931. doi: 10.1016/j.theriogenology.2010.04.017 Catt, J. (1996). Intracytoplasmic sperm injection (ICSI) and related technology. Animal Reproduction Science, 42(1-4), 239-250. Doi: 10.1016/0378-4320(96)01481-9 Chen, S., & Seidel, G. (1997). Bovine oocyte activation after intracytoplasmic sperm injection. Theriogenology, 47(1), 248. Doi: 10.1016/s0093-691x(97)82375-8 Chen, S., & Seidel, G. (1997). Effects of oocyte activation and treatment of spermatozoa on embryonic development following intracytoplasmic sperm injection in cattle. Theriogenology, 48(8), 1265-1273. Doi: 10.1016/s0093-691x(97)00369-5 Chen, S., Lien,Y.,Cheng,Y.,Chen,H.,Yang,Y.2001. Vitrification of mouse oocytes using closed pulled straws achieves a high survival and preserves good patterns of meiotic spindles, compared with conventional straws, open pulled straws and grids. Human reproduction, 16:2350-2356. Galli, C., Crotti, G., Notari, C., & Lazzari, G. (1999). High rate of activation and fertilisation following intracytoplasmatic sperm injection (ICSI) in cattle. Theriogenology, 51(1), 355. Doi: 10.1016/s0093-691x(99)91914-3 Galli, C., Vassiliev, I., Lagutina, I., Galli, A., & Lazzari, G. (2003). Bovine embryo development following ICSI: effect of activation, sperm capacitation and pre-treatment with dithiothreitol. Theriogenology, 60(8), 1467-1480. Doi: 10.1016/s0093-691x(03)00133-x Görlach, A. (1999). Transferencia de embriones en el ganado vacuno. España: Editorial Acribia, S.A. Graciano, J. (2014). Injeção Intracitoplasmática de Espermatozoides (ICSI) (Pregrado). Universidade De Brasília Faculdade De Agronomia E Medicina Veterinária Hagemann LJ, Hillery-Weinhold FL, Leibfried Rutledge ML, First NL. 1995. Activation of murine oocytes with Ca2+ ionophore and cycloheximide. J Exp Zool, 271 57-61. Hanada, A., Nagase, H. (1981). Effects sperm preincubation in rabbit uterus and of imidazole on the penetration of zona-free hamster eggs by bull and board spermatozoa in vitro. J. Anim. Reprod. 25:113-118. Harshan, H. (2008). Intracytoplasmic sperm injection: method and application in veterinary science. Veterinary World, 1(1), 23-27. Retrieved from http://www.veterinaryworld.org/2008/January/Sperm.pdf Hidalgo Ordóñez, C.O., Tamargo Miguel, C., Díez Monforte, C. Análisis del semen bovino. Tecnología Agroalimentaria. 2005 ; (2): 39-43 Horiuchi, T., Emuta, C., Yamauchi, Y., Oikawa, T., Numabe, T., & Yanagimachi, R. (2002). Birth of normal calves after intracytoplasmic sperm injection of bovine oocytes: a methodological approach. Theriogenology, 57(3), 1013-1024. Doi: 10.1016/s0093-691x(01)00701-4 Horiuchi, T., Emuta, C., Yamauchi, Y., Yamada, M., Oikawa, T., & Numabe, T. (1999). Intracytoplasmic sperm injection (ICSI) of bull spermatozoa immobilized by tail scoring. Theriogenology, 51(1), 357. Doi: 10.1016/s0093-691x(99)91916-7 Iritani, A., Niwa, K. (1977). Capacitation of bull espermatozoa and in vitro fertilization of cattle follicular oocytes matured in culture. J. Reprod. Fert. 50:119-121. Jo, H., Bang, J., Kim, S., Choi, B., Jin, J., & Kim, H. Et al. (2014). Production of female bovine embryos with sex-sorted sperm using intracytoplasmic sperm injection: Efficiency and in vitro developmental competence. Theriogenology, 81(5), 675-682.e1. Doi: 10.1016/j.theriogenology.2013.11.010 Kant, V., Parmar, M., Pant, C., Karuppanas, K., Mili, B., & Upadhyay, D. (2013). Intracytoplasmic Sperm Injection (ICSI) and its Applications in Veterinary Sciences: An Overview. Science International, 1(8), 266-270. Doi: 10.17311/sciintl.2013.266.270 Katayose, H., Yanagida, K., Shinoki, T., Kawahara, T., Horiuchi, T., & Sato, A. (1999). Efficient injection of bull spermatozoa into oocytes using a piezo-driven pipette. Theriogenology, 52(7), 1215-1224. Doi: 10.1016/s0093-691x(99)00213-7 Keefer, C., Younis, A., & Brackett, B. (1990). Cleavage development of bovine oocytes fertilized by sperm injection. Molecular Reproduction And Development, 25(3), 281-285. Doi: 10.1002/mrd.1080250310 Keskintepe L, Pacholczyk G, Machnicka A, Norris K, Curuk M. A, Khan I, Brackett B. G. 2002. Bovine blastocyst development from oocytes injected with freeze-dried spermatozoa. Biol Reprod, 67 409-415. Keskintepe, L., & Brackett, B. (2000). Cryopreservation of bovine blastocysts obtained by intracytoplasmic sperm injection. Theriogenology, 53(5), 1041-1052. Doi: 10.1016/s0093-691x(00)00250-8 Kubiak JZ, Weber M, de Pennart H, Winston NJ, Maro B. 1993. The metaphase II arrest in mouse oocytes is controlled through microtubule-dependent destruction of cyclin B in the presence of CSF. EMBO J, 12 3773-3778. Lee, J., Chang, H., Wu, H., Liu, S., Wang, C., Chu, C., & Shen, P. (2015). Effects of sperm pretreatment and embryo activation methods on the development of bovine embryos produced by intracytoplasmic sperm injection. Reproductive Biology, 15(3), 154-162. Doi: 10.1016/j.repbio.2015.07.001 Leibfried, L., Crttser, E.S., Parrish, J.J., First, N.L. (1989). In vitro maturation and fertilization of bovine oocytes. Theriogenology, 31:61-74. Leyva O, C., Barreras S, A., & Varizanga D, M. (1999). Transferencia no quirúrgica de embriones en el ganado bovino (1st ed.). Universidad Autónoma de Baja California. Malcuit, C., Maserati, M., Takahashi, Y., Page, R., & Fissore, R. (2006). Intracytoplasmic sperm injection in the bovine induces abnormal [Ca2+]iresponses and oocyte activation. Reproduction, Fertility And Development, 18(2), 39. Doi: 10.1071/rd05131 Martínez Martínez, Y. (2013). Análisis de la Morfología Ovocitaria En Bovina Previa a Fecundación In Vitro (Maestria). Universidad de Oviedo Máster Universitario en Biología y Tecnología de la Reproducción. Martins, C.F., Silva, A.E.D.F., Rumpf, R. (2002). Injeção Intracitoplasmática de Células Espermáticas e suas Aplicações na Reprodução dos Bovinos. Documentos / Embrapa Recursos Genéticos e Biotecnologia p 39. ISSN 0102-0110; n. 84 Medvedev, S., Bossak, N., Eckert, J., Lucas-Hahn, A., Niemann, H., & Johnson, L. (1997). Intracytoplasmic sperm injection (ICSI) with flow cytometrically sorted Y-chromosome bearing bovine sperm. Theriogenology, 47(1), 270. Doi: 10.1016/s0093-691x(97)82397-7 Mezzalira, J., Ohlweiler, L., Klein, N., Brum, D., Leivas, F., & Mezzalira, A. (2017). Intracytoplasmic Sperm Injection after Vitrification of Immature Oocytes in Follicular Fluid Increases Bovine Embryo Production. Acta Scientiae Veterinariae, 45(1), 7. Doi: 10.22456/1679-9216.80790 Moisyadi, S., Kaminski, J., & Yanagimachi, R. (2009). Use of intracytoplasmic sperm injection (ICSI) to generate transgenic animals. Comparative Immunology, Microbiology And Infectious Diseases, 32(2), 47-60. doi: 10.1016/j.cimid.2008.05.003 Ohlweiler, L., Brum, D., Leivas, F., Moyses, A., Ramos, R., & Klein, N. Et al. (2013). Intracytoplasmic sperm injection improves in vitro embryo production from poor quality bovine oocytes. Theriogenology, 79(5), 778-783. Doi: 10.1016/j.theriogenology.2012.12.002 Oikawa, T., Takada, N., Kikuchi, T., Numabe, T., Takenaka, M., & Horiuchi, T. (2005). Evaluation of activation treatments for blastocyst production and birth of viable calves following bovine intracytoplasmic sperm injection. Animal Reproduction Science, 86(3-4), 187-194. Doi: 10.1016/j.anireprosci.2004.07.003 Otoi, T., Tachkawa, S., Kondo, S., Suaki, T. (1993). Effects of different lots of semen from the lame bu¡] on in vitro development of bovine oocytes fertilized in vitro. Theriogenology 39:713-718. Pérez, F. And Pérez, F. 1985. Dilución del esperma. Reproducción Animal, Inseminación Artificial y Transplante de Embriones. Editorial científicomédica, Barcelona, España. Capítulo 6: 215-249. Presicce GA, Yang X. 1994. Nuclear dynamics of parthenogenesis of bovine oocytes matured in vitro for 20 and 40 hours and activated with combined ethanol and cycloheximide treatment. Mol Reprod Dev, 37 61-68. Rahman, A. (2010). Intracytoplasmic Sperm Injection-Revolution in Human and Animal Assisted Reproduction: A Review. Biotechnology(Faisalabad), 9(4), 392-410. Doi: 10.3923/biotech.2010.392.410 Reyes S., M. (1994). Fecundación in vitro en bovinos: Avances en el manejo de gamentos. Avances en Ciencias Veterinarias, 9(1). Rho GJ, Wu B, Kawarsky S, Leibo SP, Betteridge KJ. 1998b. Activation regimens to prepare bovine oocytes for intracytoplasmic sperm injection. Mol Reprod Dev, 50 485-492. Rho, G., Kawarsky, S., Johnson, W., Kochhar, K., & Betteridge, K. (1998). Sperm and Oocyte Treatments to Improve the Formation of Male and Female Pronuclei and Subsequent Development Following Intracytoplasmic Sperm Injection into Bovine Oocytes1. Biology Of Reproduction, 59(4), 918-924. Doi: 10.1095/biolreprod59.4.918 Salamone, D., Canel, N., & Rodríguez, M. (2017). Intracytoplasmic sperm injection in domestic and wild mammals. Reproduction, 154(6), F111-F124. Doi: 10.1530/rep-17-0357 Sekhavati, M., Shadanloo, F., Hosseini, M., Tahmoorespur, M., Nasiri, M., Hajian, M., & Nasr-Esfahani, M. (2012). Improved Bovine ICSI Outcomes by Sperm Selected after Combined Heparin-Glutathione Treatment. Cellular Reprogramming, 14(4), 295-304. Doi: 10.1089/cell.2012.0014 Shamsuddin, M., Niwa, K., Larsson, B., & Rodriguez-Martinez, H. (1996). In vitro maturation and fertilization of bovine oocytes. Reprod Dom Anim, 31, 613-622. Shi, D.S., Lu, K.H., Gordon, J. (1990). Effect of bull on fertilization of bovine oocytes and there subsequent development in vitro. Theriogenology. 33:324. Suttner, R., Zakhartchenko, V., Stojkovic, P., Müller, S., Alberio, R., & Medjugorac, I. Et al. (2000). Intracytoplasmic sperm injection in bovine: Effects of oocyte activation, sperm pretreatment and injection technique. Theriogenology, 54(6), 935-948. Doi: 10.1016/s0093-691x(00)00403-9 Tocharus, C., Kitiyanant, Y., & Pavasuthipaisit, K. (1996). Fertilization and subsequent development of bovine embryos after intracytoplasmic sperm injection (ICSI) using different injection pipettes. Theriogenology, 45(1), 302. Doi: 10.1016/0093-691x(96)84775-3 Wallach, E., Palermo, G., Cohen, J., & Rosenwaks, Z. (1996). Intracytoplasmic sperm injection: a powerful tool to overcome fertilization failure. Fertility And Sterility, 65(5), 899-908. Doi: 10.1016/s0015-0282(16)58257-0 Zambrano, F., Aguila, L., Arias, M., Sánchez, R., & Felmer, R. (2016). Improved preimplantation development of bovine ICSI embryos generated with spermatozoa pretreated with membrane-destabilizing agents lysolecithin and Triton X-100. Theriogenology, 86(6), 1489-1497. Doi: 10.1016/j.theriogenology.2016.05.007.
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spelling	Trujillo Piso, Dunia YiselaCasas Silva, Santiago E.2019-04-03T13:43:41Z2019-04-03T13:43:41Z2018-06https://hdl.handle.net/20.500.12494/8133Casas Silva. S. E. (2018). Inyección intracitoplasmática del espermatozoide en la reproducción asistida en bovinos: revisión literaria. (Tesis de pregrado). Recuperado de: http://repository.ucc.edu.co/handle/ucc/8133La inyección intracitoplasmática del espermatozoide (ICSI) es una técnica reciente de la reproducción asistida que se ha prescrito en la mayoría de los mamíferos con infertilidad en el macho pero que ha tenido bastante acogida y evolución desde su descubrimiento. Aunque en humanos se ha logrado sobrepasar varias barreras, en bovinos se han vivido ciertas dificultades relacionadas con el desarrollo embrionario y fetal debido a las características de sus gametos y al desarrollo de la técnica. Es un desafío para el Médico veterinario realizar estos procedimientos en campo, pero debido a su eficacia, se ha logrado continuar con la técnica estudiando sus mejoras y realizando acciones que lleven a optimizar el proceso desde la recolección de ovocitos hasta su transferencia embrionaria y posterior preñez. El objetivo de esta revisión es la actualización de la información más reciente al respecto de la técnica ICSI, sus ventajas y desventajas en las producciones ganaderas, para convertirse en una herramienta literaria para los médicos veterinarios dedicados a la reproducción bovina.Intracytoplasmic sperm injection (ICSI) is a recent technique of assisted reproduction that has been prescribed in mammals with male infertility but that has been quite welcome since its evolution and discover. Although in humans several barriers have been overcome, in bovines certain difficulties related to embryonic and fetal development have been experienced due to the characteristics of their gametes and the development of the technique. It is a challenge for the veterinarian to perform these procedures in the field, but due to its effectiveness, it has been possible to continue with the technique studying its improvements and performing actions that lead to optimize the process from the collection of oocytes to its embryo transfer and subsequent pregnancy. The objective of this work is the compilation of more recent information about ICSI, its advantages and disadvantages in livestock production, in a practical way to become a literary tool for veterinarians dedicated to bovine reproductionsantiago.casas@campusucc.edu.co24Universidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Medicina Veterinaría y Zootecnia, IbaguéMedicina veterinaria y zootecniaIbaguéICSISemen sexadoReproducción bovinaEmbrionesOvocitoInfertilidadTG 2018 MVZ 8133ICSISexed semenBovine reproductionEmbryosOocyteInfertilityInyección intracitoplasmática del espermatozoide en la reproducción asistida en bovinos: revisión literaria.Trabajo de grado - Pregradohttp://purl.org/coar/resource_type/c_7a1finfo:eu-repo/semantics/bachelorThesisinfo:eu-repo/semantics/acceptedVersionAtribución – No comercial – Sin Derivarinfo:eu-repo/semantics/closedAccesshttp://purl.org/coar/access_right/c_14cbAbdalla H, Shimoda M, Hirabayashi M, Hochi S. 2009. A combined treatment of ionomycin with ethanol improves blastocyst development of bovine oocytes harvested from stored ovaries and microinjected with spermatozoa. Theriogenology, 72 453-460.Abdalla, H., Shimoda, M., Hara, H., Morita, H., Kuwayama, M., Hirabayashi, M., & Hochi, S. (2010). Vitrification of ICSI- and IVF-derived bovine blastocysts by minimum volume cooling procedure: effect of developmental stage and age. Theriogenology, 74(6), 1028-1035. Doi: 10.1016/j.theriogenology.2010.04.033Águila, L., Zambrano, F., Arias, M., & Felmer, R. (2017). Sperm capacitation pretreatment positively impacts bovine intracytoplasmic sperm injection. Molecular Reproduction And Development, 84(7), 649-659. Doi: 10.1002/mrd.22834Ajduk, A., Yamauchi, Y., & Ward, M. (2006). Sperm Chromatin Remodeling after Intracytoplasmic Sperm Injection Differs from That of In Vitro Fertilization1. Biology Of Reproduction, 75(3), 442-451. Doi: 10.1095/biolreprod.106.053223Arias Cea, M. (2013). Optimizacion de la Técnica de Inyección Intracitoplasmatica de Espermatozoides (ICSI) en Bovinos y Evaluación de la Calidad de los Embriones Generados (Doctorado). Universidad de la Frontera Facultad de Ciencias Agropecuarias y Forestales.Arias, M., Risopatrón, J., Sánchez, R., & Felmer, R. (2015). Intracytoplasmic sperm injection affects embryo developmental potential and gene expression in cattle. Reproductive Biology, 15(1), 34-41. Doi: 10.1016/j.repbio.2014.11.001Assumpção, M.; Haipeck, k.; Lima, A.S.; Mello, M.; Oliveira, l.; Oliveira, V.;Tavares, l.; Visintin, J. Capacitação espermática In Vitro com heparina e cálcio ionóforo e sua correlação com a fertilidade em touros. Braz. J. Vet. Res. Anim. Sci 39, Numero 2: 149-156. 2002.Benavides Torres, R. (2008). Evaluación de Tres Protocolos de Capacitación Espermática Bovina In Vitro a Través de la Penetración Oocitaria (Pregrado). 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Inyección intracitoplasmática del espermatozoide en la reproducción asistida en bovinos: revisión literaria.

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