Venom from Opisthacanthus elatus scorpion of Colombia, could bemore hemolytic and less neurotoxic than thought
We report the first biochemical, biological, pharmacological and partial proteomic characterizationstudies of the Opisthancanthus elatus venom (Gervais, 1844) from Colombia.
- Autores:
-
Estrada Gómez, Sebastián
Vargas Muñoz, Leidy Johana
Saldarriaga Córdoba, Mónica María
Quintana Castillo, Juan Carlos
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2015
- Institución:
- Universidad Cooperativa de Colombia
- Repositorio:
- Repositorio UCC
- Idioma:
- OAI Identifier:
- oai:repository.ucc.edu.co:20.500.12494/1333
- Acceso en línea:
- https://hdl.handle.net/20.500.12494/1333
- Palabra clave:
- Toxinologia
Opisthacanthus elatus
Scorpion
- Rights
- openAccess
- License
- Licencia CC
| id |
COOPER2_93dd5fe7a59e374f338b7b7dad666e26 |
|---|---|
| oai_identifier_str |
oai:repository.ucc.edu.co:20.500.12494/1333 |
| network_acronym_str |
COOPER2 |
| network_name_str |
Repositorio UCC |
| repository_id_str |
|
| dc.title.spa.fl_str_mv |
Venom from Opisthacanthus elatus scorpion of Colombia, could bemore hemolytic and less neurotoxic than thought |
| title |
Venom from Opisthacanthus elatus scorpion of Colombia, could bemore hemolytic and less neurotoxic than thought |
| spellingShingle |
Venom from Opisthacanthus elatus scorpion of Colombia, could bemore hemolytic and less neurotoxic than thought Toxinologia Opisthacanthus elatus Scorpion |
| title_short |
Venom from Opisthacanthus elatus scorpion of Colombia, could bemore hemolytic and less neurotoxic than thought |
| title_full |
Venom from Opisthacanthus elatus scorpion of Colombia, could bemore hemolytic and less neurotoxic than thought |
| title_fullStr |
Venom from Opisthacanthus elatus scorpion of Colombia, could bemore hemolytic and less neurotoxic than thought |
| title_full_unstemmed |
Venom from Opisthacanthus elatus scorpion of Colombia, could bemore hemolytic and less neurotoxic than thought |
| title_sort |
Venom from Opisthacanthus elatus scorpion of Colombia, could bemore hemolytic and less neurotoxic than thought |
| dc.creator.fl_str_mv |
Estrada Gómez, Sebastián Vargas Muñoz, Leidy Johana Saldarriaga Córdoba, Mónica María Quintana Castillo, Juan Carlos |
| dc.contributor.advisor.none.fl_str_mv |
Vargas Muñoz, Leidy Johana |
| dc.contributor.author.none.fl_str_mv |
Estrada Gómez, Sebastián Vargas Muñoz, Leidy Johana Saldarriaga Córdoba, Mónica María Quintana Castillo, Juan Carlos |
| dc.subject.spa.fl_str_mv |
Toxinologia Opisthacanthus elatus Scorpion |
| topic |
Toxinologia Opisthacanthus elatus Scorpion |
| description |
We report the first biochemical, biological, pharmacological and partial proteomic characterizationstudies of the Opisthancanthus elatus venom (Gervais, 1844) from Colombia. |
| publishDate |
2015 |
| dc.date.issued.none.fl_str_mv |
2015-09-27 |
| dc.date.accessioned.none.fl_str_mv |
2017-08-23T23:47:27Z |
| dc.date.available.none.fl_str_mv |
2017-08-23T23:47:27Z |
| dc.type.none.fl_str_mv |
Artículo |
| dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
| dc.type.coar.none.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
| dc.type.coarversion.none.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.driver.none.fl_str_mv |
info:eu-repo/semantics/article |
| dc.type.version.none.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| format |
http://purl.org/coar/resource_type/c_6501 |
| status_str |
publishedVersion |
| dc.identifier.uri.none.fl_str_mv |
https://hdl.handle.net/20.500.12494/1333 |
| dc.identifier.bibliographicCitation.spa.fl_str_mv |
Estrada Gómez, S., Vargas Muñoz, L. J., Saldarriaga Córdoba, M. & Quintana Castillo, J. C. (2016). Venom from Opisthacanthus elatus scorpion of Colombia, could bemore hemolytic and less neurotoxic than thought. Acta Tropica, 153, 70-78 |
| url |
https://hdl.handle.net/20.500.12494/1333 |
| identifier_str_mv |
Estrada Gómez, S., Vargas Muñoz, L. J., Saldarriaga Córdoba, M. & Quintana Castillo, J. C. (2016). Venom from Opisthacanthus elatus scorpion of Colombia, could bemore hemolytic and less neurotoxic than thought. Acta Tropica, 153, 70-78 |
| dc.relation.isversionof.spa.fl_str_mv |
http://dx.doi.org/10.1016/j.actatropica.2015.09.019 |
| dc.rights.cc.none.fl_str_mv |
Licencia CC |
| dc.rights.accessrights.none.fl_str_mv |
info:eu-repo/semantics/openAccess |
| dc.rights.coar.none.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
| rights_invalid_str_mv |
Licencia CC http://purl.org/coar/access_right/c_abf2 |
| eu_rights_str_mv |
openAccess |
| dc.publisher.spa.fl_str_mv |
Universidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Programa de Medicina, Medellín y Envigado, Colombia, 00000 |
| dc.publisher.program.spa.fl_str_mv |
Medicina |
| dc.publisher.place.spa.fl_str_mv |
Medellín |
| institution |
Universidad Cooperativa de Colombia |
| bitstream.url.fl_str_mv |
https://repository.ucc.edu.co/bitstreams/9b2bfa28-eefe-4124-aebf-8021c06cd219/download https://repository.ucc.edu.co/bitstreams/9973be2b-1067-4622-ae70-ee639e7e6df6/download https://repository.ucc.edu.co/bitstreams/99099a0e-8117-4c68-b5f5-e05fdc86b030/download https://repository.ucc.edu.co/bitstreams/c198e2f8-9064-4e03-9159-6f5181f0f15e/download |
| bitstream.checksum.fl_str_mv |
fd073e7d94a9affe9c320b5ca27666c2 cb8f4d9b72d863ad6737f22af442cc82 0e366f66c0bbc34a13b8f3ae2d26e068 c80c23977c1b7ca81b585b7a9e8bbee2 |
| bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 MD5 |
| repository.name.fl_str_mv |
Repositorio Institucional Universidad Cooperativa de Colombia |
| repository.mail.fl_str_mv |
bdigital@metabiblioteca.com |
| _version_ |
1814247065880887296 |
| spelling |
Vargas Muñoz, Leidy JohanaEstrada Gómez, SebastiánVargas Muñoz, Leidy JohanaSaldarriaga Córdoba, Mónica MaríaQuintana Castillo, Juan Carlos2017-08-23T23:47:27Z2017-08-23T23:47:27Z2015-09-27https://hdl.handle.net/20.500.12494/1333Estrada Gómez, S., Vargas Muñoz, L. J., Saldarriaga Córdoba, M. & Quintana Castillo, J. C. (2016). Venom from Opisthacanthus elatus scorpion of Colombia, could bemore hemolytic and less neurotoxic than thought. Acta Tropica, 153, 70-78We report the first biochemical, biological, pharmacological and partial proteomic characterizationstudies of the Opisthancanthus elatus venom (Gervais, 1844) from Colombia.We report the first biochemical, biological, pharmacological and partial proteomic characterizationstudies of the Opisthancanthus elatus venom (Gervais, 1844) from Colombia. The Reverse Phase High-Performance Liquid Chromatography venom profile showed 28 main well-defined peaks, most elutingbetween 20 and 45 min (18–30% of acetonitrile, respectively). High-resolution mass analysis indicatesthe presence of 106 components ranging from 806.59742 Da to 16849.4139 Da. O. elatus venom showedhemolytic activity and hydrolyzed the specific substrate BapNa suggesting the presence of proteins withserine-protease activity. Collected RP-HPLC fractions eluting at 52.6, 55.5, 55.8, 56.2, and 63.9 min (PLA2region between 33 and 40% of acetonitrile), showed hemolytic activity and hydrolyzed the synthetic sub-strate 4-nitro-3-octanoyloxy-benzoic acid, indicating the presence of compounds with phospholipasesA2activity. These RP-HPLC fractions, showed molecular masses values up to 13978.19546 Da, corroborat-ing the possible presence of the mentioned enzymes. Tryptic digestion and MS/MS analysis showed thepresence of a phospholipase like fragment, similar to on described in other Opisthacanthus genus studies.No coagulant activity was observed. No larvicidal or antimicrobial activity was observed at concentra-tions evaluated. Lethal and toxic activity is expected at doses above 100 mg/kg, no neurotoxic effects weredetected at lower doses. In conclusion, O. elatus exhibits a venom with a predominant phospholipase A2activity than thought; mammal’s neurotoxic activity is expected above the 100 mg/kg, which is very highcompared to the venom from other neurotoxic scorpions.sebastian.estrada@udea.edu.coleidy.vargasmu@campusucc.edu.coUniversidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Programa de Medicina, Medellín y Envigado, Colombia, 00000MedicinaMedellínhttp://dx.doi.org/10.1016/j.actatropica.2015.09.019ToxinologiaOpisthacanthus elatusScorpionVenom from Opisthacanthus elatus scorpion of Colombia, could bemore hemolytic and less neurotoxic than thoughtArtículohttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionLicencia CCinfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2PublicationORIGINALO. elatus ActaT final.pdfO. elatus ActaT final.pdfapplication/pdf1334373https://repository.ucc.edu.co/bitstreams/9b2bfa28-eefe-4124-aebf-8021c06cd219/downloadfd073e7d94a9affe9c320b5ca27666c2MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-84553https://repository.ucc.edu.co/bitstreams/9973be2b-1067-4622-ae70-ee639e7e6df6/downloadcb8f4d9b72d863ad6737f22af442cc82MD52THUMBNAILO. elatus ActaT final.pdf.jpgO. elatus ActaT final.pdf.jpgIM Thumbnailimage/jpeg3988https://repository.ucc.edu.co/bitstreams/99099a0e-8117-4c68-b5f5-e05fdc86b030/download0e366f66c0bbc34a13b8f3ae2d26e068MD54TEXTO. elatus ActaT final.pdf.txtO. elatus ActaT final.pdf.txtExtracted texttext/plain52991https://repository.ucc.edu.co/bitstreams/c198e2f8-9064-4e03-9159-6f5181f0f15e/downloadc80c23977c1b7ca81b585b7a9e8bbee2MD5320.500.12494/1333oai:repository.ucc.edu.co:20.500.12494/13332024-08-10 22:44:02.359open.accesshttps://repository.ucc.edu.coRepositorio Institucional Universidad Cooperativa de Colombiabdigital@metabiblioteca.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 |