Características histológicas de piel cultivada in vitro

In vitro skin culture have been used in the regeneration of skin wound (chronic ulcers and burns), and for genodermatosis treatment. In our country there is a high patient number with chronic ulcers and 2319 burned in a period of 10 years. Conventional treatment generates long hospitalization stays...

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Autores:: Arango Rodríguez, Martha Ligia
Chamorro Jiménez, Clara Ibel
Restrepo Múnera, Luz Marina
Correa Londoño, Luis Alfonso
Henao Pérez, Julieta

Tipo de recurso:: Article of journal

Fecha de publicación:: 2010

Institución:: Universidad Cooperativa de Colombia

Repositorio:: Repositorio UCC

Idioma:: spa

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repository_id_str
dc.title.spa.fl_str_mv	Características histológicas de piel cultivada in vitro
dc.title.translated.none.fl_str_mv	Histological characteristics of skin culture in vitro
title	Características histológicas de piel cultivada in vitro
spellingShingle	Características histológicas de piel cultivada in vitro Skin Substitute Keratinocytes Fibroblast Cell culture Regenerative medicine Skin substitute Keratinocytes Fibroblasts Cell culture Regenerative medicine
title_short	Características histológicas de piel cultivada in vitro
title_full	Características histológicas de piel cultivada in vitro
title_fullStr	Características histológicas de piel cultivada in vitro
title_full_unstemmed	Características histológicas de piel cultivada in vitro
title_sort	Características histológicas de piel cultivada in vitro
dc.creator.fl_str_mv	Arango Rodríguez, Martha Ligia Chamorro Jiménez, Clara Ibel Restrepo Múnera, Luz Marina Correa Londoño, Luis Alfonso Henao Pérez, Julieta
dc.contributor.author.none.fl_str_mv	Arango Rodríguez, Martha Ligia Chamorro Jiménez, Clara Ibel Restrepo Múnera, Luz Marina Correa Londoño, Luis Alfonso Henao Pérez, Julieta
dc.contributor.researcher.none.fl_str_mv	Henao Pérez, Julieta
dc.subject.other.none.fl_str_mv	Skin Substitute Keratinocytes Fibroblast Cell culture Regenerative medicine
topic	Skin Substitute Keratinocytes Fibroblast Cell culture Regenerative medicine Skin substitute Keratinocytes Fibroblasts Cell culture Regenerative medicine
dc.subject.proposal.eng.fl_str_mv	Skin substitute Keratinocytes Fibroblasts Cell culture Regenerative medicine
description	In vitro skin culture have been used in the regeneration of skin wound (chronic ulcers and burns), and for genodermatosis treatment. In our country there is a high patient number with chronic ulcers and 2319 burned in a period of 10 years. Conventional treatment generates long hospitalization stays and high costs. We established culture conditions of keratinocytes and fibroblasts expansion, to generate a cutaneous substitute in order to offer other treatment options. Skin cells were obtained from organs donors and surgical surpluses procedures. Major success was achieved in primary cultures obtained from 40-year-old younger donors samples (65%), compared with older donors (33%). In one cutaneous substitute produced with these cells, was demonstrated thatkeratinocytes and fibroblasts, presented functional, structural and morphologic characteristics similar to normal skin. Cutaneous substitute besides preserve normal skin functional and structural characteristic, compared with other similar imported products, our cutaneous substitute, showed many advantages in terms of costs, manipulation and stability.
publishDate	2010
dc.date.issued.none.fl_str_mv	2010-12-15
dc.date.accessioned.none.fl_str_mv	2024-02-26T19:20:38Z
dc.date.available.none.fl_str_mv	2024-02-26T19:20:38Z
dc.type.none.fl_str_mv	Artículo
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dc.identifier.citation.none.fl_str_mv	Arango Rodríguez, M., Chamorro, C. I., Restrepo, L. M., Correa, L. A. y Henao, J. Características histológicas de piel cultivada in vitro,. Oct-Dic. 2009, Vol. 90 N° 4.Recuperado: https://rad-online.org.ar/2010/01/01/caracteristicas-histologicas-de-piel-cultivada-in-vitro/https://hdl.handle.net/20.500.12494/55069
dc.identifier.issn.none.fl_str_mv	ISSN 1851-300x
dc.identifier.uri.none.fl_str_mv	https://hdl.handle.net/20.500.12494/55069
identifier_str_mv	Arango Rodríguez, M., Chamorro, C. I., Restrepo, L. M., Correa, L. A. y Henao, J. Características histológicas de piel cultivada in vitro,. Oct-Dic. 2009, Vol. 90 N° 4.Recuperado: https://rad-online.org.ar/2010/01/01/caracteristicas-histologicas-de-piel-cultivada-in-vitro/https://hdl.handle.net/20.500.12494/55069 ISSN 1851-300x
url	https://hdl.handle.net/20.500.12494/55069
dc.language.iso.none.fl_str_mv	spa
language	spa
dc.relation.citationedition.none.fl_str_mv	https://rad-online.org.ar/2010/01/01/caracteristicas-histologicas-de-piel-cultivada-in-vitro/
dc.relation.citationendpage.none.fl_str_mv	200 p.
dc.relation.citationissue.none.fl_str_mv	Número 4
dc.relation.citationstartpage.none.fl_str_mv	190 p.
dc.relation.citationvolume.none.fl_str_mv	Vol. 90
dc.relation.ispartofjournal.none.fl_str_mv	Revista Argentina de Dermatología
dc.relation.references.none.fl_str_mv	Inoue H, Oshima H, Matsuzaki K y Kumagai N. Application for regenerative medicine of epithelial cell culture-vistas of cultured epithelium. Congenit Anom (Kyoto) 2006; 46 (3): 129-134 Jensen TG. Cutaneous gene therapy. Ann Med 2007; 39 (2): 108-115. Bello YM, Falabella AF y Eaglstein WH. Tissue-engineered skin. Current status in wound healing. Am J Clin Dermatol 2001; 2 (5): 305-313. Muhart M, McFalls S, Kirsner R, Kerdel F y Eaglstein WH. Bioengineered skin. Lancet 1997; 350 (9085): 1142. Matouskova E, Broz L, Stolbova V, Klein L, Konigova R y Vesely P. Human alloge neic keratinocytes cultured on acellular xenodermis: the use in healing of burns REV ARGENT DERMATOL 2009; 90: 190-200. 199 and other skin defects. Biomed Mater Eng 2006; 16 (4): S63-S71. Gallico GG, III, O’Connor NE, Compton CC, Kehinde O y Green H. Permanent coverage of large burn wounds with autologous cultured human epithelium. N Engl J Med 1984; 311 (7): 448-451 Hu S, Kirsner RS, Falanga V, Phillips T y Eaglstein WH. Evaluation of Apligraf persistence and basement membrane restoration in donor site wounds: a pilot study. Wound Repair Regen 2006; 14 (4): 427-433. Johnsen S, Ermuth T, Tanczos E y col. Treatment of therapy-refractive ulcera cruris of various origins with autologous keratinocytes in fibrin sealant. Vasa 2005; 34 (1): 25-29. Kirsner RS, Fastenau J, Falabella A, Va lencia I, Long R y Eaglstein WH. Clinical and economic outcomes with graftskin for hard-to-heal venous leg ulcers: a single center experience. Dermatol Surg 2002; 28 (1): 81-82 Falabella AF, Schachner LA, Valencia IC y Eaglstein WH. The use of tissue-enginee red skin (Apligraf) to treat a newborn with epidermolysis bullosa. Arch Dermatol 1999; 135 (10): 1219-1222. Rheinwald JG y Green H. Serial cultiva tion of strains of human epidermal keratinocytes: the formation of keratini zing colonies from single cells. Cell 1975; 6 (3): 331-343. Compton CC, Hickerson W, Nadire K y Press W. Acceleration of skin regeneration from cultured epithelial autografts by transplantation to homograft dermis. J Burn Care Rehabil 1993; 14 (6): 653-662. Breitkreutz D, Mirancea N y Nischt R. Basement membranes in skin: unique matrix structures with diverse functions? Histochem Cell Biol 2009; 132 (1): 1-10. Butler CE, Orgill DP, Yannas IV y Compton CC. Effect of keratinocyte seeding of collagen-glycosaminoglycan membranes on the regeneration of skin in a porcine model. Plast Reconstr Surg 1998; 101 (6): 1572-1579. Butler CE, Yannas IV, Compton CC, Correia CA y Orgill DP. Comparison of cultured and uncultured keratinocytes seeded into collagen-GAG matrix for skin replace ments. Br J Plast Surg 1999; 52 (2): 127- 132 El Ghalbzouri A, Commandeur S, Rietveld MH, Mulder AA y Willemze R. Replace ment of animal-derived collagen matrix by human fibroblast-derived dermal matrix for human skin equivalent products. Biomaterials 2008. Meana A, Iglesias J, Del Rio M y col. Large surface of cultured human epithelium obtained on a dermal matrix based on live fibroblast-containing fibrin gels. Burns 1998; 24 (7): 621-630. rango M, Chamorro C, Cohen Haguenauer O, Rojas M y Restrepo LM. Human skin keratinocytes modified by a Friend-derived retroviral vector: a functional approach. Dermatol Online J 2005; 11 (2): 2 Wang JF, Olson ME, Reno CR, Wright JB y Hart DA. The pig as a model for excisional skin wound healing: characterization of the molecular and cellular biology, and bacteriology of the healing process. Comp Med 2001; 51 (4): 341-348. Horch RE, Kopp J, Kneser U, Beier J y Bach AD. Tissue engineering of cultured skin substitutes. J Cell Mol Med 2005; 9 (3): 592- 608. Gilchrest BA. In vitro assessment of keratinocyte aging. J Invest Dermatol 1983; 81 (1): 184s-189s. Jeyapalan JC y Sedivy JM. Cellular senescence and organismal aging. Mech Ageing Dev 2008; 129 (7-8): 467-474. Martin GM, Sprague CA y Epstein CJ. Replicative life-span of cultivated human cells. Effects of donor’s age, tissue, and genotype. Lab Invest 1970; 23 (1): 86-92. Nakazawa K, Kalassy M, Sahuc F, Collombel C y Damour O. Pigmented human skin equivalent—as a model of the mechanisms of control of cell-cell and cell matrix interactions. Med 1998; 36 (6): 813-820. Compton CC, Warland G y Kratz G. Melanocytes in cultured epithelial grafts are depleted with serial subcultivation and cryopreservation: implications for clinical outcome. J Burn Care Rehabil 1998; 19 (4): 330-336. Limat A, Salomon D, Carraux P, Saurat JH y Hunziker T. Human melanocytes grown in epidermal equivalents transfer their melanin to follicular outer root sheath keratinocytes. Arch Dermatol Res 1999; 291 (6): 325-332. Rheinwald JG y Green H. Formation of a keratinizing epithelium in culture by a cloned cell line derived from a teratoma. Cell 1975; 6 (3): 317-330. Rheinwald JG. Serial cultivation of nor mal human epidermal keratinocytes. Methods Cell Biol 1980; 21A: 229-254 Black AF, Bouez C, Perrier E, Schlotmann K, Chapuis F y Damour O. Optimization and characterization of an engineered human skin equivalent. Tissue Eng 2005; 11 (5-6): 723-733 Horch RE, Debus M, Wagner G y Stark GB. Cultured human keratinocytes on type I collagen membranes to reconstitute the epidermis. Tissue Eng 2000; 6 (1): 53-67 Ho G, Barbenel J y Grant MH. Effect of low level laser treatment of tissue-engineered skin substitutes: contraction of collagen lattices. J Biomed Opt 2009; 14 (3): 034002. Casasco M, Casasco A, Icaro CA, Farina A y Calligaro A. Differential distribution of elastic tissue in human natural skin and tissue-engineered skin. J Mol Histol 2004; 35 (4): 421-428 Escobar-Franco M, Henao J, Wolff M, Estrada S y Restrepo L. Tratamiento de las úlceras crónicas en los miembros inferio res con un equivalente cutáneo autólogo y desbridación con larvas de Lucilia sp. (Diptera: Calliphoridae). Reporte de un caso. Iatreia 2007; 20 (4): 397-406. Siwy BK y Compton CC. Cultured epider mis: Indiana University Medical Center’s experience. J Burn Care Rehabil 1992; 13 (1): 130-137. Eaglstein WH, Iriondo M y Laszlo K. A composite skin substitute (graftskin) for surgical wounds. A clinical experience. Dermatol Surg 1995; 21 (10): 839-843 Hernon CA, Dawson RA, Freedlander E y col. Clinical experience using cultured epithelial autografts leads to an alternative methodology for transferring skin cells from the laboratory to the patient. Regen Med 2006; 1 (6): 809-821. Llames SG, Del Rio M, Larcher F y col. Human plasma as a dermal scaffold for the generation of a completely autologous bioengineered skin. Transplantation 2004; 77 (3): 350-355. Llames S, Garcia E, Garcia V y col. Clinical results of an autologous engineered skin. Cell Tissue Bank 2006; 7 (1): 47-53 Rio MD, Larcher F, Meana A, Segovia J, Alvarez A y Jorcano J. Nonviral transfer of genes to pig primary keratinocytes. Induction of angiogenesis by composite grafts of modified keratinocytes overex pressing VEGF driven by a keratin promoter. Gene Ther 1999; 6 (10): 1734- 1741. Larcher F, Del Rio M, Serrano F y col. A cutaneous gene therapy approach to human leptin deficiencies: correction of the murine ob/ob phenotype using leptin targeted keratinocyte grafts. FASEB J 2001; 15 (9): 1529-1538 Escamez MJ, Garcia M, Larcher F y col. An in vivo model of wound healing in genetically modified skin-humanized mice. J Invest Dermatol 2004; 123 (6): 1182- 1191. Del Río M, Larcher F, Serrano F y col. A preclinical model for the analysis of genetically modified human skin in vivo. Hum Genet Ther 2002; 13 (8): 959-968. Faller C, Bracher M, Dami N y Roguet R. Predictive ability of reconstructed human epidermis equivalents for the assessment of skin irritation of cosmetics. Toxicol In Vitro 2002; 16 (5): 557-572 Frei V, Perrier E, Orly I, Huc A, Augustin C y Damour O.Activation of fibroblast metabolism in a dermal and skin equiva lent model: a screening test for activity of peptides. Int J Cosmet Sci 1998; 20 (3): 159- 173. Damour O, Augustin C y Black AF. Applications of reconstructed skin models in pharmaco-toxicological trials. Med Biol Eng Comput 1998; 36 (6): 825-832. Roguet R. The use of standardized human skin models for cutaneous pharmacotoxi cology studies. Skin Pharmacol Appl Skin Physiol 2002; 15 (1): 1-3
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spelling	Arango Rodríguez, Martha LigiaChamorro Jiménez, Clara IbelRestrepo Múnera, Luz MarinaCorrea Londoño, Luis AlfonsoHenao Pérez, JulietaHenao Pérez, Julieta2024-02-26T19:20:38Z2024-02-26T19:20:38Z2010-12-15Arango Rodríguez, M., Chamorro, C. I., Restrepo, L. M., Correa, L. A. y Henao, J. Características histológicas de piel cultivada in vitro,. Oct-Dic. 2009, Vol. 90 N° 4.Recuperado: https://rad-online.org.ar/2010/01/01/caracteristicas-histologicas-de-piel-cultivada-in-vitro/https://hdl.handle.net/20.500.12494/55069ISSN 1851-300xhttps://hdl.handle.net/20.500.12494/55069In vitro skin culture have been used in the regeneration of skin wound (chronic ulcers and burns), and for genodermatosis treatment. In our country there is a high patient number with chronic ulcers and 2319 burned in a period of 10 years. Conventional treatment generates long hospitalization stays and high costs. We established culture conditions of keratinocytes and fibroblasts expansion, to generate a cutaneous substitute in order to offer other treatment options. Skin cells were obtained from organs donors and surgical surpluses procedures. Major success was achieved in primary cultures obtained from 40-year-old younger donors samples (65%), compared with older donors (33%). In one cutaneous substitute produced with these cells, was demonstrated thatkeratinocytes and fibroblasts, presented functional, structural and morphologic characteristics similar to normal skin. Cutaneous substitute besides preserve normal skin functional and structural characteristic, compared with other similar imported products, our cutaneous substitute, showed many advantages in terms of costs, manipulation and stability.julieta.henaop@campusucc.edu.co10 p.application/pdfspaArgentinaUniversidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Medicina, Medellín y Envigadohttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://purl.org/coar/access_right/c_abf2https://rad-online.org.ar/2010/01/01/caracteristicas-histologicas-de-piel-cultivada-in-vitro/Skin SubstituteKeratinocytesFibroblastCell cultureRegenerative medicineSkin substituteKeratinocytesFibroblastsCell cultureRegenerative medicineCaracterísticas histológicas de piel cultivada in vitroHistological characteristics of skin culture in vitroArtículohttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://rad-online.org.ar/2010/01/01/caracteristicas-histologicas-de-piel-cultivada-in-vitro/200 p.Número 4190 p.Vol. 90Revista Argentina de DermatologíaInoue H, Oshima H, Matsuzaki K y Kumagai N. Application for regenerative medicine of epithelial cell culture-vistas of cultured epithelium. Congenit Anom (Kyoto) 2006; 46 (3): 129-134Jensen TG. Cutaneous gene therapy. Ann Med 2007; 39 (2): 108-115.Bello YM, Falabella AF y Eaglstein WH. Tissue-engineered skin. Current status in wound healing. Am J Clin Dermatol 2001; 2 (5): 305-313.Muhart M, McFalls S, Kirsner R, Kerdel F y Eaglstein WH. Bioengineered skin. Lancet 1997; 350 (9085): 1142.Matouskova E, Broz L, Stolbova V, Klein L, Konigova R y Vesely P. Human alloge neic keratinocytes cultured on acellular xenodermis: the use in healing of burns REV ARGENT DERMATOL 2009; 90: 190-200. 199 and other skin defects. Biomed Mater Eng 2006; 16 (4): S63-S71.Gallico GG, III, O’Connor NE, Compton CC, Kehinde O y Green H. Permanent coverage of large burn wounds with autologous cultured human epithelium. N Engl J Med 1984; 311 (7): 448-451Hu S, Kirsner RS, Falanga V, Phillips T y Eaglstein WH. Evaluation of Apligraf persistence and basement membrane restoration in donor site wounds: a pilot study. Wound Repair Regen 2006; 14 (4): 427-433.Johnsen S, Ermuth T, Tanczos E y col. Treatment of therapy-refractive ulcera cruris of various origins with autologous keratinocytes in fibrin sealant. Vasa 2005; 34 (1): 25-29.Kirsner RS, Fastenau J, Falabella A, Va lencia I, Long R y Eaglstein WH. Clinical and economic outcomes with graftskin for hard-to-heal venous leg ulcers: a single center experience. Dermatol Surg 2002; 28 (1): 81-82Falabella AF, Schachner LA, Valencia IC y Eaglstein WH. The use of tissue-enginee red skin (Apligraf) to treat a newborn with epidermolysis bullosa. Arch Dermatol 1999; 135 (10): 1219-1222.Rheinwald JG y Green H. Serial cultiva tion of strains of human epidermal keratinocytes: the formation of keratini zing colonies from single cells. Cell 1975; 6 (3): 331-343.Compton CC, Hickerson W, Nadire K y Press W. Acceleration of skin regeneration from cultured epithelial autografts by transplantation to homograft dermis. J Burn Care Rehabil 1993; 14 (6): 653-662.Breitkreutz D, Mirancea N y Nischt R. Basement membranes in skin: unique matrix structures with diverse functions? Histochem Cell Biol 2009; 132 (1): 1-10.Butler CE, Orgill DP, Yannas IV y Compton CC. Effect of keratinocyte seeding of collagen-glycosaminoglycan membranes on the regeneration of skin in a porcine model. Plast Reconstr Surg 1998; 101 (6): 1572-1579.Butler CE, Yannas IV, Compton CC, Correia CA y Orgill DP. Comparison of cultured and uncultured keratinocytes seeded into collagen-GAG matrix for skin replace ments. Br J Plast Surg 1999; 52 (2): 127- 132El Ghalbzouri A, Commandeur S, Rietveld MH, Mulder AA y Willemze R. Replace ment of animal-derived collagen matrix by human fibroblast-derived dermal matrix for human skin equivalent products. Biomaterials 2008.Meana A, Iglesias J, Del Rio M y col. Large surface of cultured human epithelium obtained on a dermal matrix based on live fibroblast-containing fibrin gels. Burns 1998; 24 (7): 621-630.rango M, Chamorro C, Cohen Haguenauer O, Rojas M y Restrepo LM. Human skin keratinocytes modified by a Friend-derived retroviral vector: a functional approach. Dermatol Online J 2005; 11 (2): 2Wang JF, Olson ME, Reno CR, Wright JB y Hart DA. The pig as a model for excisional skin wound healing: characterization of the molecular and cellular biology, and bacteriology of the healing process. Comp Med 2001; 51 (4): 341-348.Horch RE, Kopp J, Kneser U, Beier J y Bach AD. Tissue engineering of cultured skin substitutes. J Cell Mol Med 2005; 9 (3): 592- 608.Gilchrest BA. In vitro assessment of keratinocyte aging. J Invest Dermatol 1983; 81 (1): 184s-189s.Jeyapalan JC y Sedivy JM. Cellular senescence and organismal aging. Mech Ageing Dev 2008; 129 (7-8): 467-474.Martin GM, Sprague CA y Epstein CJ. Replicative life-span of cultivated human cells. Effects of donor’s age, tissue, and genotype. Lab Invest 1970; 23 (1): 86-92.Nakazawa K, Kalassy M, Sahuc F, Collombel C y Damour O. Pigmented human skin equivalent—as a model of the mechanisms of control of cell-cell and cell matrix interactions. Med 1998; 36 (6): 813-820.Compton CC, Warland G y Kratz G. Melanocytes in cultured epithelial grafts are depleted with serial subcultivation and cryopreservation: implications for clinical outcome. J Burn Care Rehabil 1998; 19 (4): 330-336.Limat A, Salomon D, Carraux P, Saurat JH y Hunziker T. Human melanocytes grown in epidermal equivalents transfer their melanin to follicular outer root sheath keratinocytes. Arch Dermatol Res 1999; 291 (6): 325-332.Rheinwald JG y Green H. Formation of a keratinizing epithelium in culture by a cloned cell line derived from a teratoma. Cell 1975; 6 (3): 317-330.Rheinwald JG. Serial cultivation of nor mal human epidermal keratinocytes. Methods Cell Biol 1980; 21A: 229-254Black AF, Bouez C, Perrier E, Schlotmann K, Chapuis F y Damour O. Optimization and characterization of an engineered human skin equivalent. Tissue Eng 2005; 11 (5-6): 723-733Horch RE, Debus M, Wagner G y Stark GB. Cultured human keratinocytes on type I collagen membranes to reconstitute the epidermis. Tissue Eng 2000; 6 (1): 53-67Ho G, Barbenel J y Grant MH. Effect of low level laser treatment of tissue-engineered skin substitutes: contraction of collagen lattices. J Biomed Opt 2009; 14 (3): 034002.Casasco M, Casasco A, Icaro CA, Farina A y Calligaro A. Differential distribution of elastic tissue in human natural skin and tissue-engineered skin. J Mol Histol 2004; 35 (4): 421-428Escobar-Franco M, Henao J, Wolff M, Estrada S y Restrepo L. Tratamiento de las úlceras crónicas en los miembros inferio res con un equivalente cutáneo autólogo y desbridación con larvas de Lucilia sp. (Diptera: Calliphoridae). Reporte de un caso. Iatreia 2007; 20 (4): 397-406.Siwy BK y Compton CC. Cultured epider mis: Indiana University Medical Center’s experience. J Burn Care Rehabil 1992; 13 (1): 130-137.Eaglstein WH, Iriondo M y Laszlo K. A composite skin substitute (graftskin) for surgical wounds. A clinical experience. Dermatol Surg 1995; 21 (10): 839-843Hernon CA, Dawson RA, Freedlander E y col. Clinical experience using cultured epithelial autografts leads to an alternative methodology for transferring skin cells from the laboratory to the patient. Regen Med 2006; 1 (6): 809-821.Llames SG, Del Rio M, Larcher F y col. Human plasma as a dermal scaffold for the generation of a completely autologous bioengineered skin. Transplantation 2004; 77 (3): 350-355.Llames S, Garcia E, Garcia V y col. Clinical results of an autologous engineered skin. Cell Tissue Bank 2006; 7 (1): 47-53Rio MD, Larcher F, Meana A, Segovia J, Alvarez A y Jorcano J. Nonviral transfer of genes to pig primary keratinocytes. Induction of angiogenesis by composite grafts of modified keratinocytes overex pressing VEGF driven by a keratin promoter. Gene Ther 1999; 6 (10): 1734- 1741.Larcher F, Del Rio M, Serrano F y col. A cutaneous gene therapy approach to human leptin deficiencies: correction of the murine ob/ob phenotype using leptin targeted keratinocyte grafts. FASEB J 2001; 15 (9): 1529-1538Escamez MJ, Garcia M, Larcher F y col. An in vivo model of wound healing in genetically modified skin-humanized mice. J Invest Dermatol 2004; 123 (6): 1182- 1191.Del Río M, Larcher F, Serrano F y col. A preclinical model for the analysis of genetically modified human skin in vivo. Hum Genet Ther 2002; 13 (8): 959-968.Faller C, Bracher M, Dami N y Roguet R. Predictive ability of reconstructed human epidermis equivalents for the assessment of skin irritation of cosmetics. Toxicol In Vitro 2002; 16 (5): 557-572Frei V, Perrier E, Orly I, Huc A, Augustin C y Damour O.Activation of fibroblast metabolism in a dermal and skin equiva lent model: a screening test for activity of peptides. Int J Cosmet Sci 1998; 20 (3): 159- 173.Damour O, Augustin C y Black AF. Applications of reconstructed skin models in pharmaco-toxicological trials. Med Biol Eng Comput 1998; 36 (6): 825-832.Roguet R. The use of standardized human skin models for cutaneous pharmacotoxi cology studies. 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Características histológicas de piel cultivada in vitro

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