Profiling analysis of circulating microRNA in peripheral blood of patients with class IV lupus nephritis
Renal involvement in Systemic Lupus Erythematous (SLE) patients is one of the leading causes of morbidity and a significant contributor to mortality. It’s estimated that nearly 50% of SLE individuals develop kidney disease in the first year of the diagnosis. Class IV lupus nephritis (LN-IV) is the c...
- Autores:
-
Navarro Quiroz, Roberto Carlos
Navarro Quiroz, Elkin Antonio
Pacheco Lugo, Lisandro
Lorenzi, Hernan
España Puccini, Pierine
Díaz Olmos, Yirys
Almendrales, Lisneth
Olave Jaller, Valeria
Gonzalez Torres, Henry
Diaz Perez, Anderson
Dominguez, Alex
Iglesias, Antonio
García, Raul
Aroca Martínez, Gustavo
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2017
- Institución:
- Universidad Cooperativa de Colombia
- Repositorio:
- Repositorio UCC
- Idioma:
- OAI Identifier:
- oai:repository.ucc.edu.co:20.500.12494/5989
- Acceso en línea:
- https://hdl.handle.net/20.500.12494/5989
- Palabra clave:
- Systemic Lupus Erythematous
Lupus nephritis
- Rights
- openAccess
- License
- http://purl.org/coar/access_right/c_abf2
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dc.title.spa.fl_str_mv |
Profiling analysis of circulating microRNA in peripheral blood of patients with class IV lupus nephritis |
title |
Profiling analysis of circulating microRNA in peripheral blood of patients with class IV lupus nephritis |
spellingShingle |
Profiling analysis of circulating microRNA in peripheral blood of patients with class IV lupus nephritis Systemic Lupus Erythematous Lupus nephritis |
title_short |
Profiling analysis of circulating microRNA in peripheral blood of patients with class IV lupus nephritis |
title_full |
Profiling analysis of circulating microRNA in peripheral blood of patients with class IV lupus nephritis |
title_fullStr |
Profiling analysis of circulating microRNA in peripheral blood of patients with class IV lupus nephritis |
title_full_unstemmed |
Profiling analysis of circulating microRNA in peripheral blood of patients with class IV lupus nephritis |
title_sort |
Profiling analysis of circulating microRNA in peripheral blood of patients with class IV lupus nephritis |
dc.creator.fl_str_mv |
Navarro Quiroz, Roberto Carlos Navarro Quiroz, Elkin Antonio Pacheco Lugo, Lisandro Lorenzi, Hernan España Puccini, Pierine Díaz Olmos, Yirys Almendrales, Lisneth Olave Jaller, Valeria Gonzalez Torres, Henry Diaz Perez, Anderson Dominguez, Alex Iglesias, Antonio García, Raul Aroca Martínez, Gustavo |
dc.contributor.author.none.fl_str_mv |
Navarro Quiroz, Roberto Carlos Navarro Quiroz, Elkin Antonio Pacheco Lugo, Lisandro Lorenzi, Hernan España Puccini, Pierine Díaz Olmos, Yirys Almendrales, Lisneth Olave Jaller, Valeria Gonzalez Torres, Henry Diaz Perez, Anderson Dominguez, Alex Iglesias, Antonio García, Raul Aroca Martínez, Gustavo |
dc.subject.spa.fl_str_mv |
Systemic Lupus Erythematous Lupus nephritis |
topic |
Systemic Lupus Erythematous Lupus nephritis |
description |
Renal involvement in Systemic Lupus Erythematous (SLE) patients is one of the leading causes of morbidity and a significant contributor to mortality. It’s estimated that nearly 50% of SLE individuals develop kidney disease in the first year of the diagnosis. Class IV lupus nephritis (LN-IV) is the class of lupus nephritis most common in Colombian patients with SLE. Altered miRNAs expression levels have been reported in human autoimmune diseases including lupus. Variations in the expression pattern of peripheral blood circulating miRNAs specific for this class of lupus nephritis could be correlated with the pathophysiological status of this group of individuals. The aim of this study was to evaluate the relative abundance of circulating microRNAs in peripheral blood from Colombian patients with LN-IV. Circulating miRNAs in plasma of patients with diagnosis of LN-IV were compared with individuals without renal involvement (LNN group) and healthy individuals (CTL group). Total RNA was extracted from 10 ml of venous blood and subsequently sequenced using Illumina. The sequences were processed and these were analyzed using miRBase and Ensembl databases. Differential gene expression analysis was carried out with edgeR and functional analysis were done with DIANA-miRPath. Analysis was carried out using as variables of selection fold change (≥2 o ≤-2) and false discovery rate (0.05). We identified 24 circulating microRNAs with differential abundance between LN-IV and CTL groups, fourteen of these microRNAs are described for the first time to lupus nephritis (hsa-miR-589-3p, hsa-miR-1260b, hsa-miR-4511, hsa-miR-485-5p, hsa-miR-584-5p, hsa-miR-543, hsa-miR-153-3p, hsa-miR-6087, hsa-miR-3942-5p, hsa-miR-7977, hsa-miR-323b-3p, hsa-miR-4732-3p and hsa-miR-6741-3p). These changes in the abundance of miRNAs could be interpreted as alterations in the miRNAs-mRNA regulatory network in the pathogenesis of LN, preceding the clinical onset of the disease. The findings thus contribute to understanding the disease process and are likely to pave the way towards identifying disease biomarkers for early diagnosis of LN. |
publishDate |
2017 |
dc.date.issued.none.fl_str_mv |
2017-11-14 |
dc.date.accessioned.none.fl_str_mv |
2018-10-30T19:27:38Z |
dc.date.available.none.fl_str_mv |
2018-09-01 2018-10-30T19:27:38Z |
dc.type.none.fl_str_mv |
Artículo |
dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
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http://purl.org/coar/resource_type/c_6501 |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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info:eu-repo/semantics/article |
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publishedVersion |
dc.identifier.uri.none.fl_str_mv |
https://hdl.handle.net/20.500.12494/5989 |
dc.identifier.bibliographicCitation.spa.fl_str_mv |
Navarro Quiroz E, Pacheco Lugo L, Navarro Quiroz R, Lorenzi H, España Puccini P, Díaz Olmos Y, et al. (2017) Profiling analysis of circulating microRNA in peripheral blood of patients with class IV lupus nephritis. PLoS ONE 12(11): e0187973. https://doi.org/10.1371/journal.pone.0187973 |
url |
https://hdl.handle.net/20.500.12494/5989 |
identifier_str_mv |
Navarro Quiroz E, Pacheco Lugo L, Navarro Quiroz R, Lorenzi H, España Puccini P, Díaz Olmos Y, et al. (2017) Profiling analysis of circulating microRNA in peripheral blood of patients with class IV lupus nephritis. PLoS ONE 12(11): e0187973. https://doi.org/10.1371/journal.pone.0187973 |
dc.relation.isversionof.spa.fl_str_mv |
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0187973 |
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info:eu-repo/semantics/openAccess |
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http://purl.org/coar/access_right/c_abf2 |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://purl.org/coar/access_right/c_abf2 |
dc.publisher.spa.fl_str_mv |
Universidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Medicina, Santa Marta |
dc.publisher.program.spa.fl_str_mv |
Medicina |
dc.publisher.place.spa.fl_str_mv |
Santa Marta |
institution |
Universidad Cooperativa de Colombia |
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Navarro Quiroz, Roberto CarlosNavarro Quiroz, Elkin AntonioPacheco Lugo, LisandroLorenzi, HernanEspaña Puccini, PierineDíaz Olmos, YirysAlmendrales, LisnethOlave Jaller, ValeriaGonzalez Torres, HenryDiaz Perez, AndersonDominguez, AlexIglesias, AntonioGarcía, RaulAroca Martínez, Gustavo2018-10-30T19:27:38Z2018-09-012018-10-30T19:27:38Z2017-11-14https://hdl.handle.net/20.500.12494/5989Navarro Quiroz E, Pacheco Lugo L, Navarro Quiroz R, Lorenzi H, España Puccini P, Díaz Olmos Y, et al. (2017) Profiling analysis of circulating microRNA in peripheral blood of patients with class IV lupus nephritis. PLoS ONE 12(11): e0187973. https://doi.org/10.1371/journal.pone.0187973Renal involvement in Systemic Lupus Erythematous (SLE) patients is one of the leading causes of morbidity and a significant contributor to mortality. It’s estimated that nearly 50% of SLE individuals develop kidney disease in the first year of the diagnosis. Class IV lupus nephritis (LN-IV) is the class of lupus nephritis most common in Colombian patients with SLE. Altered miRNAs expression levels have been reported in human autoimmune diseases including lupus. Variations in the expression pattern of peripheral blood circulating miRNAs specific for this class of lupus nephritis could be correlated with the pathophysiological status of this group of individuals. The aim of this study was to evaluate the relative abundance of circulating microRNAs in peripheral blood from Colombian patients with LN-IV. Circulating miRNAs in plasma of patients with diagnosis of LN-IV were compared with individuals without renal involvement (LNN group) and healthy individuals (CTL group). Total RNA was extracted from 10 ml of venous blood and subsequently sequenced using Illumina. The sequences were processed and these were analyzed using miRBase and Ensembl databases. Differential gene expression analysis was carried out with edgeR and functional analysis were done with DIANA-miRPath. Analysis was carried out using as variables of selection fold change (≥2 o ≤-2) and false discovery rate (0.05). We identified 24 circulating microRNAs with differential abundance between LN-IV and CTL groups, fourteen of these microRNAs are described for the first time to lupus nephritis (hsa-miR-589-3p, hsa-miR-1260b, hsa-miR-4511, hsa-miR-485-5p, hsa-miR-584-5p, hsa-miR-543, hsa-miR-153-3p, hsa-miR-6087, hsa-miR-3942-5p, hsa-miR-7977, hsa-miR-323b-3p, hsa-miR-4732-3p and hsa-miR-6741-3p). These changes in the abundance of miRNAs could be interpreted as alterations in the miRNAs-mRNA regulatory network in the pathogenesis of LN, preceding the clinical onset of the disease. The findings thus contribute to understanding the disease process and are likely to pave the way towards identifying disease biomarkers for early diagnosis of LN.Abstract -- Introduction -- Materials and methods -- Results -- Discussion -- Supporting information -- Acknowledgments -- Referencesroberto.navarroq@campusucc.edu.coUniversidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Medicina, Santa MartaMedicinaSanta Martahttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0187973Systemic Lupus ErythematousLupus nephritisProfiling analysis of circulating microRNA in peripheral blood of patients with class IV lupus nephritisArtículohttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://orcid.org/0000-0001-7362-7769info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2PublicationORIGINALProfiling analysis of circulating microRNA in peripheral blood of patients with class IV lupus nephritis.pdfProfiling analysis of circulating microRNA in peripheral blood of patients with class IV lupus nephritis.pdfapplication/pdf1862478https://repository.ucc.edu.co/bitstreams/272728b2-ac4f-4b64-ae12-1f021d0ce2b3/downloadb593dbe95a7183e1086b161c32899941MD51FORMATO DE LICENCIA DE USO DE OBRA Profiling analysis of circulating microRNA in peripheral blood of patients with class IV.pdfFORMATO DE LICENCIA DE USO DE OBRA Profiling analysis of circulating microRNA in peripheral blood of patients with class 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