Profiling analysis of circulating microRNA in peripheral blood of patients with class IV lupus nephritis

Renal involvement in Systemic Lupus Erythematous (SLE) patients is one of the leading causes of morbidity and a significant contributor to mortality. It’s estimated that nearly 50% of SLE individuals develop kidney disease in the first year of the diagnosis. Class IV lupus nephritis (LN-IV) is the c...

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Autores:
Navarro Quiroz, Roberto Carlos
Navarro Quiroz, Elkin Antonio
Pacheco Lugo, Lisandro
Lorenzi, Hernan
España Puccini, Pierine
Díaz Olmos, Yirys
Almendrales, Lisneth
Olave Jaller, Valeria
Gonzalez Torres, Henry
Diaz Perez, Anderson
Dominguez, Alex
Iglesias, Antonio
García, Raul
Aroca Martínez, Gustavo
Tipo de recurso:
Article of journal
Fecha de publicación:
2017
Institución:
Universidad Cooperativa de Colombia
Repositorio:
Repositorio UCC
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OAI Identifier:
oai:repository.ucc.edu.co:20.500.12494/5989
Acceso en línea:
https://hdl.handle.net/20.500.12494/5989
Palabra clave:
Systemic Lupus Erythematous
Lupus nephritis
Rights
openAccess
License
http://purl.org/coar/access_right/c_abf2
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dc.title.spa.fl_str_mv Profiling analysis of circulating microRNA in peripheral blood of patients with class IV lupus nephritis
title Profiling analysis of circulating microRNA in peripheral blood of patients with class IV lupus nephritis
spellingShingle Profiling analysis of circulating microRNA in peripheral blood of patients with class IV lupus nephritis
Systemic Lupus Erythematous
Lupus nephritis
title_short Profiling analysis of circulating microRNA in peripheral blood of patients with class IV lupus nephritis
title_full Profiling analysis of circulating microRNA in peripheral blood of patients with class IV lupus nephritis
title_fullStr Profiling analysis of circulating microRNA in peripheral blood of patients with class IV lupus nephritis
title_full_unstemmed Profiling analysis of circulating microRNA in peripheral blood of patients with class IV lupus nephritis
title_sort Profiling analysis of circulating microRNA in peripheral blood of patients with class IV lupus nephritis
dc.creator.fl_str_mv Navarro Quiroz, Roberto Carlos
Navarro Quiroz, Elkin Antonio
Pacheco Lugo, Lisandro
Lorenzi, Hernan
España Puccini, Pierine
Díaz Olmos, Yirys
Almendrales, Lisneth
Olave Jaller, Valeria
Gonzalez Torres, Henry
Diaz Perez, Anderson
Dominguez, Alex
Iglesias, Antonio
García, Raul
Aroca Martínez, Gustavo
dc.contributor.author.none.fl_str_mv Navarro Quiroz, Roberto Carlos
Navarro Quiroz, Elkin Antonio
Pacheco Lugo, Lisandro
Lorenzi, Hernan
España Puccini, Pierine
Díaz Olmos, Yirys
Almendrales, Lisneth
Olave Jaller, Valeria
Gonzalez Torres, Henry
Diaz Perez, Anderson
Dominguez, Alex
Iglesias, Antonio
García, Raul
Aroca Martínez, Gustavo
dc.subject.spa.fl_str_mv Systemic Lupus Erythematous
Lupus nephritis
topic Systemic Lupus Erythematous
Lupus nephritis
description Renal involvement in Systemic Lupus Erythematous (SLE) patients is one of the leading causes of morbidity and a significant contributor to mortality. It’s estimated that nearly 50% of SLE individuals develop kidney disease in the first year of the diagnosis. Class IV lupus nephritis (LN-IV) is the class of lupus nephritis most common in Colombian patients with SLE. Altered miRNAs expression levels have been reported in human autoimmune diseases including lupus. Variations in the expression pattern of peripheral blood circulating miRNAs specific for this class of lupus nephritis could be correlated with the pathophysiological status of this group of individuals. The aim of this study was to evaluate the relative abundance of circulating microRNAs in peripheral blood from Colombian patients with LN-IV. Circulating miRNAs in plasma of patients with diagnosis of LN-IV were compared with individuals without renal involvement (LNN group) and healthy individuals (CTL group). Total RNA was extracted from 10 ml of venous blood and subsequently sequenced using Illumina. The sequences were processed and these were analyzed using miRBase and Ensembl databases. Differential gene expression analysis was carried out with edgeR and functional analysis were done with DIANA-miRPath. Analysis was carried out using as variables of selection fold change (≥2 o ≤-2) and false discovery rate (0.05). We identified 24 circulating microRNAs with differential abundance between LN-IV and CTL groups, fourteen of these microRNAs are described for the first time to lupus nephritis (hsa-miR-589-3p, hsa-miR-1260b, hsa-miR-4511, hsa-miR-485-5p, hsa-miR-584-5p, hsa-miR-543, hsa-miR-153-3p, hsa-miR-6087, hsa-miR-3942-5p, hsa-miR-7977, hsa-miR-323b-3p, hsa-miR-4732-3p and hsa-miR-6741-3p). These changes in the abundance of miRNAs could be interpreted as alterations in the miRNAs-mRNA regulatory network in the pathogenesis of LN, preceding the clinical onset of the disease. The findings thus contribute to understanding the disease process and are likely to pave the way towards identifying disease biomarkers for early diagnosis of LN.
publishDate 2017
dc.date.issued.none.fl_str_mv 2017-11-14
dc.date.accessioned.none.fl_str_mv 2018-10-30T19:27:38Z
dc.date.available.none.fl_str_mv 2018-09-01
2018-10-30T19:27:38Z
dc.type.none.fl_str_mv Artículo
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dc.identifier.bibliographicCitation.spa.fl_str_mv Navarro Quiroz E, Pacheco Lugo L, Navarro Quiroz R, Lorenzi H, España Puccini P, Díaz Olmos Y, et al. (2017) Profiling analysis of circulating microRNA in peripheral blood of patients with class IV lupus nephritis. PLoS ONE 12(11): e0187973. https://doi.org/10.1371/journal.pone.0187973
url https://hdl.handle.net/20.500.12494/5989
identifier_str_mv Navarro Quiroz E, Pacheco Lugo L, Navarro Quiroz R, Lorenzi H, España Puccini P, Díaz Olmos Y, et al. (2017) Profiling analysis of circulating microRNA in peripheral blood of patients with class IV lupus nephritis. PLoS ONE 12(11): e0187973. https://doi.org/10.1371/journal.pone.0187973
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dc.publisher.spa.fl_str_mv Universidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Medicina, Santa Marta
dc.publisher.program.spa.fl_str_mv Medicina
dc.publisher.place.spa.fl_str_mv Santa Marta
institution Universidad Cooperativa de Colombia
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spelling Navarro Quiroz, Roberto CarlosNavarro Quiroz, Elkin AntonioPacheco Lugo, LisandroLorenzi, HernanEspaña Puccini, PierineDíaz Olmos, YirysAlmendrales, LisnethOlave Jaller, ValeriaGonzalez Torres, HenryDiaz Perez, AndersonDominguez, AlexIglesias, AntonioGarcía, RaulAroca Martínez, Gustavo2018-10-30T19:27:38Z2018-09-012018-10-30T19:27:38Z2017-11-14https://hdl.handle.net/20.500.12494/5989Navarro Quiroz E, Pacheco Lugo L, Navarro Quiroz R, Lorenzi H, España Puccini P, Díaz Olmos Y, et al. (2017) Profiling analysis of circulating microRNA in peripheral blood of patients with class IV lupus nephritis. PLoS ONE 12(11): e0187973. https://doi.org/10.1371/journal.pone.0187973Renal involvement in Systemic Lupus Erythematous (SLE) patients is one of the leading causes of morbidity and a significant contributor to mortality. It’s estimated that nearly 50% of SLE individuals develop kidney disease in the first year of the diagnosis. Class IV lupus nephritis (LN-IV) is the class of lupus nephritis most common in Colombian patients with SLE. Altered miRNAs expression levels have been reported in human autoimmune diseases including lupus. Variations in the expression pattern of peripheral blood circulating miRNAs specific for this class of lupus nephritis could be correlated with the pathophysiological status of this group of individuals. The aim of this study was to evaluate the relative abundance of circulating microRNAs in peripheral blood from Colombian patients with LN-IV. Circulating miRNAs in plasma of patients with diagnosis of LN-IV were compared with individuals without renal involvement (LNN group) and healthy individuals (CTL group). Total RNA was extracted from 10 ml of venous blood and subsequently sequenced using Illumina. The sequences were processed and these were analyzed using miRBase and Ensembl databases. Differential gene expression analysis was carried out with edgeR and functional analysis were done with DIANA-miRPath. Analysis was carried out using as variables of selection fold change (≥2 o ≤-2) and false discovery rate (0.05). We identified 24 circulating microRNAs with differential abundance between LN-IV and CTL groups, fourteen of these microRNAs are described for the first time to lupus nephritis (hsa-miR-589-3p, hsa-miR-1260b, hsa-miR-4511, hsa-miR-485-5p, hsa-miR-584-5p, hsa-miR-543, hsa-miR-153-3p, hsa-miR-6087, hsa-miR-3942-5p, hsa-miR-7977, hsa-miR-323b-3p, hsa-miR-4732-3p and hsa-miR-6741-3p). These changes in the abundance of miRNAs could be interpreted as alterations in the miRNAs-mRNA regulatory network in the pathogenesis of LN, preceding the clinical onset of the disease. The findings thus contribute to understanding the disease process and are likely to pave the way towards identifying disease biomarkers for early diagnosis of LN.Abstract -- Introduction -- Materials and methods -- Results -- Discussion -- Supporting information -- Acknowledgments -- Referencesroberto.navarroq@campusucc.edu.coUniversidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Medicina, Santa MartaMedicinaSanta Martahttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0187973Systemic Lupus ErythematousLupus nephritisProfiling analysis of circulating microRNA in peripheral blood of patients with class IV lupus 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