Protection against Schistosoma mansoni infection using a Fasciola hepatica-derived fatty acid binding protein from different delivery systems

Background: Schistosomiasis is a water-borne disease afflicting over 261 million people in many areas of the developing countries with high morbidity and mortality. The control relies mainly on treatment with praziquantel. Fatty acid binding proteins (FABP) have demonstrated high levels of immune-protection against trematode infections. This study reports the immunoprotection induced by cross-reacting Fasciola hepatica FABP, native (nFh12) and recombinantly expressed using two different expression systems Escherichia coli (rFh15) and baculovirus (rFh15b) against Schistosoma mansoni infection. Methods: BALB/c mice were vaccinated with native nFh12 or recombinant rFh15 and rFh15 FABP from F. hepatica formulated in adjuvant adaptation (ADAD) system with natural or chemical synthesised immunomodulators (PAL and AA0029) and then challenged with 150 cercariae of S. mansoni. Parasite burden, hepatic lesions and antibody response were studied in vaccination trials. Furthermore differences between rFh15 and rFh15b immunological responses (cytokine production, splenocyte population and antibody levels) were studied. Results: Vaccination with nFh12 induced significant reductions in worm burden (83 %), eggs in tissues (82-92 %) and hepatic lesions (85 %) compared to infected controls using PAL. Vaccination with rFh15 showed lower total worm burden (56-64 %), eggs in the liver (21-61 %), eggs in the gut (30-77 %) and hepatic damage (67-69 %) using PAL and AA0029 as immunomodulators. In contrast, mice vaccinated with rFh15b showed only reductions in eggs trapped in the liver and intestine (53 and 60 %, respectively), and hepatic lesions (45 %). We observed a significant rise in TNFa, IL-6, IL-2, IL-4 and high antibody response (IgG, IgG1, IgG2a, IgM and IgE) in mice immunised with either rFh15 or rFh15b. Moreover, mice immunised with rFh15b showed an increase in IFN? and a decrease in B220 cells compared to untreated mice, and less production of IgG1 and IgM than in mice immunised by rFh15. Conclusions: Higher level of protection is obtained by using Fasciola hepatica-derived FABP protein against Schistosoma mansoni infection. Native FABP is more effective than both recombinant systems. It could be due to post-translational modifications or FABP isoform or changes in the recombinant proteins. © 2016 Vicente et al.

• Autores: Vicente B.; López-Abán J.; Rojas Caraballo, Jose Vicente; Del Olmo E.; Fernández-Soto P.; Muro A.
• Tipo de recurso: Article of journal
• Fecha de publicación: 2016
• Institución: Universidad Cooperativa de Colombia
• Repositorio: Repositorio UCC
• OAI Identifier: oai:repository.ucc.edu.co:20.500.12494/41841
• Acceso en línea: https://doi.org/10.16925/sp.v9i18.548; https://revistas.unab.edu.co/index.php/medunab/article/view/2272/2806; https://hdl.handle.net/20.500.12494/41841
• Palabra clave: fatty acid binding protein; gamma interferon; immunoglobulin E antibody; immunoglobulin G antibody; immunoglobulin G1 antibody; immunoglobulin G2a antibody; immunoglobulin M antibody; immunomodulating agent; interleukin 2; interleukin 4; interleukin 6; Schistosoma vaccine; tumor necrosis factor alpha; fatty acid binding protein; helminth antibody; helminth protein; interleukin 2; interleukin 4; recombinant protein; animal experiment; animal model; animal tissue; antibody response; Article; Baculoviridae; cercaria; controlled study; cytokine production; Escherichia coli; Fasciola hepatica; female; intestine; mouse; nonhuman; parasite load; schistosomiasis mansoni; spleen cell; animal; Bagg albino mouse; cross protection; drug delivery system; Fasciola hepatica; genetics; human; immunology; parasitology; schistosomiasis mansoni; vaccination; Animals; Antibodies; Helminth; Cross Protection; Drug Delivery Systems; Fasciola hepatica; Fatty Acid-Binding Proteins; Female; Helminth Proteins; H
• Rights: closedAccess
• License: http://purl.org/coar/access_right/c_14cb