Molecular surveillance of Trypanosoma spp. reveals different clinical and epidemiological characteristics associated with the infection in three creole cattle breeds from Colombia

En América del Sur, Colombia es el tercer productor ganadero con aproximadamente 28,8 millones de cabezas de ganado, de las cuales el ganado criollo colombiano representa alrededor del 1% de la población ganadera. La Tripanosomiasis Animal (AT) es uno de los problemas más críticos en la industria ga...

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Autores:: Jaimes Dueñez, Jeiczon Elim
Mogollón Waltero, Edgar Mauricio
Arias Landazábal, José Norberto
Rangel Pachon, David
Jiménez Leaño, Ángela Patricia
Mejía Jaramillo, Ana
Triana Chavez, Omar

Tipo de recurso:: Article of investigation

Fecha de publicación:: 2021

Institución:: Universidad Cooperativa de Colombia

Repositorio:: Repositorio UCC

Idioma:

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repository_id_str
dc.title.spa.fl_str_mv	Molecular surveillance of Trypanosoma spp. reveals different clinical and epidemiological characteristics associated with the infection in three creole cattle breeds from Colombia
title	Molecular surveillance of Trypanosoma spp. reveals different clinical and epidemiological characteristics associated with the infection in three creole cattle breeds from Colombia
spellingShingle	Molecular surveillance of Trypanosoma spp. reveals different clinical and epidemiological characteristics associated with the infection in three creole cattle breeds from Colombia Animal Trypanosomiasis Prevalencia Razas Criollas Análisis clínico Animal Trypanosomiasis Prevalence Creole breeds Clinical analysis
title_short	Molecular surveillance of Trypanosoma spp. reveals different clinical and epidemiological characteristics associated with the infection in three creole cattle breeds from Colombia
title_full	Molecular surveillance of Trypanosoma spp. reveals different clinical and epidemiological characteristics associated with the infection in three creole cattle breeds from Colombia
title_fullStr	Molecular surveillance of Trypanosoma spp. reveals different clinical and epidemiological characteristics associated with the infection in three creole cattle breeds from Colombia
title_full_unstemmed	Molecular surveillance of Trypanosoma spp. reveals different clinical and epidemiological characteristics associated with the infection in three creole cattle breeds from Colombia
title_sort	Molecular surveillance of Trypanosoma spp. reveals different clinical and epidemiological characteristics associated with the infection in three creole cattle breeds from Colombia
dc.creator.fl_str_mv	Jaimes Dueñez, Jeiczon Elim Mogollón Waltero, Edgar Mauricio Arias Landazábal, José Norberto Rangel Pachon, David Jiménez Leaño, Ángela Patricia Mejía Jaramillo, Ana Triana Chavez, Omar
dc.contributor.author.none.fl_str_mv	Jaimes Dueñez, Jeiczon Elim Mogollón Waltero, Edgar Mauricio Arias Landazábal, José Norberto Rangel Pachon, David Jiménez Leaño, Ángela Patricia Mejía Jaramillo, Ana Triana Chavez, Omar
dc.subject.spa.fl_str_mv	Animal Trypanosomiasis Prevalencia Razas Criollas Análisis clínico
topic	Animal Trypanosomiasis Prevalencia Razas Criollas Análisis clínico Animal Trypanosomiasis Prevalence Creole breeds Clinical analysis
dc.subject.other.spa.fl_str_mv	Animal Trypanosomiasis Prevalence Creole breeds Clinical analysis
description	En América del Sur, Colombia es el tercer productor ganadero con aproximadamente 28,8 millones de cabezas de ganado, de las cuales el ganado criollo colombiano representa alrededor del 1% de la población ganadera. La Tripanosomiasis Animal (AT) es uno de los problemas más críticos en la industria ganadera, reduciendo su producción en cerca de un 30 %. Considerando la escasez de información para comprender las características epidemiológicas de la AT en bovinos criollos colombianos, el presente estudio reporta la prevalencia molecular y los rasgos clínicos asociados con la infección de Trypanosoma spp. en tres razas criollas colombianas. De 2019 a 2020 se diseñó una vigilancia transversal en fincas del centro y occidente de Colombia para evaluar las características mencionadas en las razas Casanare˜no, Chino Santandereano y Criollo Sanmartinero. El análisis molecular mostró una prevalencia de AT del 60,2 % (IC 95 % = 54,2 % - 66,2 %). La población Chino Santandereano presentó el mayor valor (Trypanosoma spp., 75,2 %, T. theileri 59,6 % y T. evansi 15,6 %), seguida por Casanare˜no (Trypanosoma spp., 65,3 %, T. theileri 38,6 %, T. evansi 24,0 % y T. vivax 5,3 %) y sanmartinero (Trypanosoma spp., 33,3 %, T. theileri 24,0 % y T. evansi 9,3 %). Características como la raza, la edad y el sistema de alimentación se asociaron significativamente con la prevalencia de AT (P < 0,05). Además, se observó un nivel bajo de proteínas totales en suero durante la infección por T. evansi en Sanmartinero (P < 0.05). Hasta donde sabemos, esta es la primera encuesta transversal que evalúa mediante métodos moleculares la infección de Trypanosoma spp. en razas criollas colombianas, mostrando variaciones significativas en la prevalencia y signos clínicos asociados a la infección. Estos resultados sugieren diferentes grados de tripanotolerancia en estas razas, así como un posible efecto de variables ambientales sobre la prevalencia y características clínicas asociadas a la infección. Las implicaciones epidemiológicas y económicas de estos hallazgos se discuten aquí.
publishDate	2021
dc.date.issued.none.fl_str_mv	2021-06-23
dc.date.accessioned.none.fl_str_mv	2022-09-14T17:31:33Z
dc.date.available.none.fl_str_mv	2022-09-14T17:31:33Z
dc.type.none.fl_str_mv	Artículos Científicos
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dc.identifier.issn.spa.fl_str_mv	0167-5877
dc.identifier.uri.spa.fl_str_mv	https://doi.org/10.1016/j.prevetmed.2021.105414
dc.identifier.uri.none.fl_str_mv	https://hdl.handle.net/20.500.12494/46387
dc.identifier.bibliographicCitation.spa.fl_str_mv	Jeiczon Jaimes-Dueñez, Edgar Mogollón-Waltero, Norberto Árias-Landazabal, David Rangel-Pachon, Angela Jimenez-Leaño, Ana Mejia-Jaramillo, Omar Triana-Chávez, Molecular surveillance of Trypanosoma spp. reveals different clinical and epidemiological characteristics associated with the infection in three creole cattle breeds from Colombia, Preventive Veterinary Medicine, Volume 193, 2021, 105414, ISSN 0167-5877, https://doi.org/10.1016/j.prevetmed.2021.105414. (https://www.sciencedirect.com/science/article/pii/S0167587721001586)-https://repository.ucc.edu.co/handle/20.500.12494/46387
identifier_str_mv	0167-5877 Jeiczon Jaimes-Dueñez, Edgar Mogollón-Waltero, Norberto Árias-Landazabal, David Rangel-Pachon, Angela Jimenez-Leaño, Ana Mejia-Jaramillo, Omar Triana-Chávez, Molecular surveillance of Trypanosoma spp. reveals different clinical and epidemiological characteristics associated with the infection in three creole cattle breeds from Colombia, Preventive Veterinary Medicine, Volume 193, 2021, 105414, ISSN 0167-5877, https://doi.org/10.1016/j.prevetmed.2021.105414. (https://www.sciencedirect.com/science/article/pii/S0167587721001586)-https://repository.ucc.edu.co/handle/20.500.12494/46387
url	https://doi.org/10.1016/j.prevetmed.2021.105414 https://hdl.handle.net/20.500.12494/46387
dc.relation.isversionof.spa.fl_str_mv	https://www.sciencedirect.com/science/article/pii/S0167587721001586
dc.relation.ispartofjournal.spa.fl_str_mv	Preventive Veterinary Medicine
dc.relation.references.spa.fl_str_mv	Amato, B., Mira, F., Di Marco Lo Presti, V., Guercio, A., Russotto, L., Gucciardi, F., Vitale, M., Lena, A., Loria, G.R., Puleio, R., Cannella, V., 2019. A case of bovine trypanosomiasis caused by Trypanosoma theileri in Sicily, Italy. Parasitol. Res. 118 (9), 2723–2727. https://doi.org/10.1007/s00436-019-06390-y. Epub 2019 Jul 13. PMID: 31302757. Amorim, L.S., Wenceslau, A.A., Carvalho, F.S., Carneiro, P.L., Albuquerque, G.R., 2014. Bovine babesiosis and anaplasmosis complex: diagnosis and evaluation of the risk factors from Bahia, Brazil. Rev. Bras. Parasitol. Vet. 23 (3), 328–336. Anosa, V.O., Isoun, T.T., 1976. Serum proteins, blood and plasma volumes in experimental Trypanosoma vivax infections of sheep and goats. Trop. Anim. Health Prod. 8 (1), 14–19. https://doi.org/10.1007/BF02383360. Baldissera, M.D., Sagrillo, M.R., de S´a, M.F., Grando, T.H., Souza, C.F., de Brum, G.F., da Luz, S.C., Oliveira, S.S., De Mello, A.L., Nascimento, K., Tatsch, E., Moresco, R.N., da Silva, A.S., Monteiro, S.G., 2016. Relationship between DNA damage in liver, heart, spleen and total blood cells and disease pathogenesis of infected rats by Trypanosoma evansi. Exp. Parasitol. 161, 12–19. https://doi.org/10.1016/j.exppara.2015.12.008 Berthier, D., Peylhard, M., Dayo, G.K., Flori, L., Sylla, S., Bolly, S., Sakande, H., Chantal, I., Thevenon, S., 2015. A comparison of phenotypic traits related to trypanotolerance in five west african cattle breeds highlights the value of shorthorn taurine breeds. PLoS One 10 (5), e0126498. https://doi.org/10.1371/journal. pone.0126498. Biryomumaisho, S., Christopher, R., 2003. Serum biochemical changes in experimental Trypanosoma congolense and Trypanosoma brucei infection in Small East Africa goats. In: Veterinarski Arhiv, Vol. 73(3, pp. 167–180 Boakye, D.A., Tang, J., Truc, P., Merriweather, A., Unnasch, T.R., 1999. Identification of bloodmeals in haematophagous Diptera by cytochrome B heteroduplex analysis. Med. Vet. Entomol. 13 (3), 282–287 Cortez, A.P., Rodrigues, A.C., Garcia, H.A., Neves, L., Batista, J.S., Bengaly, Z., Paiva, F., Teixeira, M.M., 2009. Cathepsin L-like genes of Trypanosoma vivax from Africa and South America–characterization, relationships and diagnostic implications. Mol. Cell. Probes 23 (1), 44–51. https://doi.org/10.1016/j.mcp.2008.11.003 d’Ieteren, G.D., Authi´e, E., Wissocq, N., Murray, M., 1998. Trypanotolerance, an option for sustainable livestock production in areas at risk from trypanosomosis. Rev. Sci. Technol. 17 (1), 154–175 Delgado, J.V., Martínez, A.M., Acosta, A., Alvarez, L.A., Armstrong, E., Camacho, E., Ca˜n´on, J., Cort´es, O., Dunner, S., Landi, V., Marques, J.R., Martín-Burriel, I., Martínez, O.R., Martínez, R.D., Melucci, L., Mu˜noz, J.E., Penedo, M.C., Postiglioni, A., Quir´oz, J., Rodellar, C., Sponenberg, P., Uffo, O., Ulloa-Arvizu, R., Vega-Pla, J.L., Villalobos, A., Zambrano, D., Zaragoza, P., Gama, L.T., Ginja, C., 2012. Genetic characterization of Latin-American Creole cattle using microsatellite markers. Anim. Genet. 43 (1), 2–10. https://doi.org/10.1111/j.1365- 2052.2011.02207.x. Desquesnes, M., 2004. Livestock Trypanosomoses and their Vectors in Latin America, 1 ed., Vol. 1. World organisation for animal health Desquesnes, M., Holzmuller, P., Lai, D.H., Dargantes, A., Lun, Z.R., Jittaplapong, S., 2013. Trypanosoma evansi and surra: a review and perspectives on origin, history, distribution, taxonomy, morphology, hosts, and pathogenic effects. Biomed Res. Int. 2013, 194176. https://doi.org/10.1155/2013/194176 Fidelis Junior, O.L., Sampaio, P.H., Machado, R.Z., Andr´e, M.R., Marques, L.C., Cadioli, F.A., 2016. Evaluation of clinical signs, parasitemia, hematologic and biochemical changes in cattle experimentally infected with Trypanosoma vivax. Rev. Bras. Parasitol. Vet. 25 (1), 69–81. https://doi.org/10.1590/S1984-29612016013 Garcia, H.A., Ramírez, O.J., Rodrigues, C.M., S´anchez, R.G., Bethencourt, A.M., Del M P´erez, G., Minervino, A.H., Rodrigues, A.C., Teixeira, M.M., 2016. Trypanosoma vivax in water buffalo of the Venezuelan Llanos: an unusual outbreak of wasting disease in an endemic area of typically asymptomatic infections. Vet. Parasitol. 230, 49–55. https://doi.org/10.1016/j.vetpar.2016.10.013 García, H.A., García, M.E., Zerpa, H., Zerpa, E., P´erez, G., Contreras, C.E., Vivas, I., Mendoza-Le´on, A., 2003. Detecci´on parasitol´ogica y molecular de infecciones naturales por Trypanosoma evansi y Trypanosoma vivax en búfalos de agua (Bubalus bubalis) y chigüires (Hydrochoerus hydrochaeris) en los estados Apure, Cojedes y Gu´arico, Venezuela. Rev. Fac. Cs. Vet. UCV 44, 131–14 Geysen, D., Delespaux, V., Geerts, S., 2003. PCR-RFLP using Ssu-rDNA amplification as an easy method for species-specific diagnosis of Trypanosoma species in cattle. Vet. Parasitol. 110 (3-4), 171–180 Hanotte, O., Ronin, Y., Agaba, M., Nilsson, P., Gelhaus, A., Horstmann, R., Sugimoto, Y., Kemp, S., Gibson, J., Korol, A., Soller, M., Teale, A., 2003. Mapping of quantitative trait loci controlling trypanotolerance in a cross of tolerant West African N’Dama and susceptible East African Boran cattle. Proc. Natl. Acad. Sci. U. S. A. 100 (13), 7443–7448. https://doi.org/10.1073/pnas.1232392100 Herrera, M., Soto, A., Urrego, V., Rivera, G., Zapata, M., Rios, M., 2008. Frequency of hemoparasites of bovines from Cauca and San Jorge Rivers, 2000-2005. In: Revista MVZ C´ordoba, Vol. 13, pp. 1486–1494 Holland, W.G., Claes, F., My, L.N., Thanh, N.G., Tam, P.T., Verloo, D., Büscher, P., Goddeeris, B., Vercruysse, J., 2001. A comparative evaluation of parasitological tests and a PCR for Trypanosoma evansi diagnosis in experimentally infected water buffaloes. Vet. Parasitol. 97 (1), 23–33 Hosmer, D., Lemeshow, S., 2005. Applied Logistic Regression, second edition. John Wiley & Sons. https://doi.org/10.1002/0471722146 ICA, 2020. Censo Pecuario año 2020. Instituto Colombiano Agropecuario. Jaimes-Dueñez, J., Triana-Ch´avez, O., Mejía-Jaramillo, A.M., 2017. Parasitological and molecular surveys reveal high rates of infection with vector-borne pathogens and clinical anemia signs associated with infection in cattle from two important livestock areas in Colombia. Ticks Tick. Dis. 8 (2), 290–299. https://doi.org/10.1016/j. ttbdis.2016.12.002. Jaimes-Dueñez, J., Triana-Ch´avez, O., Mejía-Jaramillo, A.M., 2018. Spatial-temporal and phylogeographic characterization ofTrypanosoma spp. in cattle (Bos taurus) and buffaloes (Bubalus bubalis) reveals transmission dynamics of these parasites in Colombia. Vet. Parasitol. 249, 30–42 Jaimes-Dueñez, J., Zapata-Zapata, C., Triana-Ch´avez, O., Mejía-Jaramillo, A.M., 2019. Evaluation of an alternative indirect-ELISA test using in vitro-propagated Trypanosoma brucei brucei whole cell lysate as antigen for the detection of anti- Trypanosoma evansi IgG in Colombian livestock. Prev. Vet. Med. 169, 104712 https://doi.org/10.1016/j.prevetmed.2019.104712 Martínez, G., 2010. National Action Plan for Conservation,improvement, and Sustainable Use of Colombian Animal Geneticresources. FAO– MADR. FAO. Muktar, Y., Asmelash, M., Mekonnen, N., 2016. Prevalence and associated risk factors of bovine trypanosomosis in Benatsemay district, SouthOmo zone, Ethiopia. In: Livestock Research for Rural Development, Vol. 28 Mwai, O., Hanotte, O., Kwon, Y.J., Cho, S., 2015. African indigenous cattle: unique genetic resources in a rapidly changing world. Asian-Australas. J. Anim. Sci. 28 (7), 911–921. https://doi.org/10.5713/ajas.15.0002R. Orenge, C.O., Munga, L., Kimwele, C.N., Kemp, S., Korol, A., Gibson, J.P., Hanotte, O., Soller, M., 2012. Trypanotolerance in N’Dama x Boran crosses under natural trypanosome challenge: effect of test-year environment, gender, and breed composition. BMC Genet. 13, 87. https://doi.org/10.1186/1471-2156-13-87 Osorio, A.L., Madruga, C.R., Desquesnes, M., Soares, C.O., Ribeiro, L.R., Costa, S.C., 2008. Trypanosoma (Duttonella) vivax: its biology, epidemiology, pathogenesis, and introduction in the New World–a review. Mem. Inst. Oswaldo Cruz 103 (1), 1–13 Otte, M.J., Abuabara, J.Y., Wells, E.A., 1994. Trypanosoma vivax in Colombia: epidemiology and production losses. Trop. Anim. Health Prod. 26 (3), 146–156 Rockett, J., Bosted, S., 2007. Veterinary Clinical Procedures in Large Animal Practice (Veterinary Technology), second ed. Cengage Learning Rosero, J., Alvarez, L., Muñoz, J., Duran, C., Rodas, A., 2012. Allelic frequency of the Kappa–Casein gene in Colombian and creole cattle breeds. In: Rev Colom Cienc Pecua, Vol. 25. Schneider, D.S., Ayres, J.S., 2008. Two ways to survive infection: what resistance and tolerance can teach us about treating infectious diseases. Nat. Rev. Immunol. 8 (11), 889–895. https://doi.org/10.1038/nri2432 Smetko, A., Soudre, A., Silbermayr, K., Müller, S., Brem, G., Hanotte, O., Boettcher, P.J., Stella, A., Meszaros, G., Wurzinger, M., Curik, I., Müller, M., Burgstaller, J., S¨olkner, J., 2015. Trypanosomosis: potential driver of selection in African cattle. Front. Genet. 6, 137. https://doi.org/10.3389/fgene.2015.00137 Torcoroma, L.G., Ardila, Y.A., Rinc´on, D., Dur´an, C., Aguilar, J.R., 2014. A new PCRRFLP for species-specific diagnosis of South American animal trypanosomiasis. Am. J. Anim. Vet. Sci. 9 (2), 128–136 Waaij, E., Hanotte, O., Arendonk, J., Kemp, S., Kennedy, D., Gibson, J., Teale, A., 2003. Population parameters for traits defining trypanotolerance in an F2 cross of N’Dama and Boran cattle. In: Livestock Production Science, Vol. 28 Yaro, M., Munyard, K.A., Stear, M.J., Groth, D.M., 2016. Combatting African Animal Trypanosomiasis (AAT) in livestock: the potential role of trypanotolerance. Vet. Parasitol. 225, 43–52. https://doi.org/10.1016/j.vetpar.2016.05.003 Zapata, R.S., Ríos, L.A., Barahona, R., Polanco, D., 2011. Estudio parasitol´ogico y entomol´ogico de tripanosomiasis en bovinos (Bos Taurus) raza Holstein de una hacienda de zona ganadera lechera de clima frio. Antioquia, Colombia. http://opac udea.udea.edu.co/query.php?1282552
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spelling	Jaimes Dueñez, Jeiczon Elim Mogollón Waltero, Edgar MauricioArias Landazábal, José Norberto Rangel Pachon, DavidJiménez Leaño, Ángela PatriciaMejía Jaramillo, AnaTriana Chavez, Omar193 (2021) 1054142022-09-14T17:31:33Z2022-09-14T17:31:33Z2021-06-230167-5877https://doi.org/10.1016/j.prevetmed.2021.105414https://hdl.handle.net/20.500.12494/46387Jeiczon Jaimes-Dueñez, Edgar Mogollón-Waltero, Norberto Árias-Landazabal, David Rangel-Pachon, Angela Jimenez-Leaño, Ana Mejia-Jaramillo, Omar Triana-Chávez, Molecular surveillance of Trypanosoma spp. reveals different clinical and epidemiological characteristics associated with the infection in three creole cattle breeds from Colombia, Preventive Veterinary Medicine, Volume 193, 2021, 105414, ISSN 0167-5877, https://doi.org/10.1016/j.prevetmed.2021.105414. (https://www.sciencedirect.com/science/article/pii/S0167587721001586)-https://repository.ucc.edu.co/handle/20.500.12494/46387En América del Sur, Colombia es el tercer productor ganadero con aproximadamente 28,8 millones de cabezas de ganado, de las cuales el ganado criollo colombiano representa alrededor del 1% de la población ganadera. La Tripanosomiasis Animal (AT) es uno de los problemas más críticos en la industria ganadera, reduciendo su producción en cerca de un 30 %. Considerando la escasez de información para comprender las características epidemiológicas de la AT en bovinos criollos colombianos, el presente estudio reporta la prevalencia molecular y los rasgos clínicos asociados con la infección de Trypanosoma spp. en tres razas criollas colombianas. De 2019 a 2020 se diseñó una vigilancia transversal en fincas del centro y occidente de Colombia para evaluar las características mencionadas en las razas Casanare˜no, Chino Santandereano y Criollo Sanmartinero. El análisis molecular mostró una prevalencia de AT del 60,2 % (IC 95 % = 54,2 % - 66,2 %). La población Chino Santandereano presentó el mayor valor (Trypanosoma spp., 75,2 %, T. theileri 59,6 % y T. evansi 15,6 %), seguida por Casanare˜no (Trypanosoma spp., 65,3 %, T. theileri 38,6 %, T. evansi 24,0 % y T. vivax 5,3 %) y sanmartinero (Trypanosoma spp., 33,3 %, T. theileri 24,0 % y T. evansi 9,3 %). Características como la raza, la edad y el sistema de alimentación se asociaron significativamente con la prevalencia de AT (P < 0,05). Además, se observó un nivel bajo de proteínas totales en suero durante la infección por T. evansi en Sanmartinero (P < 0.05). Hasta donde sabemos, esta es la primera encuesta transversal que evalúa mediante métodos moleculares la infección de Trypanosoma spp. en razas criollas colombianas, mostrando variaciones significativas en la prevalencia y signos clínicos asociados a la infección. Estos resultados sugieren diferentes grados de tripanotolerancia en estas razas, así como un posible efecto de variables ambientales sobre la prevalencia y características clínicas asociadas a la infección. Las implicaciones epidemiológicas y económicas de estos hallazgos se discuten aquí.In South America, Colombia is the third-largest livestock producer with approximately 28.8 million cattle, of which Colombian Creole cattle represent around 1% of the livestock population. Animal Trypanosomiasis (AT) is one of the most critical problems in the livestock industry, reducing its production by about 30 %. Considering the paucity of information to understand the epidemiological features of AT in Colombian Creole cattle, the present study reports the molecular prevalence and clinical traits associated with the infection of Trypanosoma spp. in three Colombian Creole breeds. From 2019 to 2020, cross-sectional surveillance in farms of central and west of Colombia was designed to evaluate the mentioned characteristics in Casanare˜no, Chino Santandereano, and Sanmartinero Creole breeds. Molecular analysis showed an AT prevalence of 60.2 % (95 % CI = 54.2 % - 66.2 %). The Chino Santandereano population presented the highest value (Trypanosoma spp., 75.2 %, T. theileri 59.6 % and T. evansi 15.6 %), followed by Casanare˜no (Trypanosoma spp., 65.3 %, T. theileri 38.6 %, T. evansi 24.0 %, and T. vivax 5.3 %) and Sanmartinero (Trypanosoma spp., 33.3 %, T. theileri 24.0 % and T. evansi 9.3 %). Features such as breeds, age, and feeding system were significantly associated with AT prevalence (P < 0.05). Additionally, a low level of serum total proteins was observed during T. evansi infection in Sanmartinero (P < 0.05). To our knowledge, this is the first cross-sectional survey that evaluates using molecular methods the infection of Trypanosoma spp. in Colombian Creole breeds, showing significant variations in the prevalence and clinical signs associated with the infection. These results suggest different degrees of trypanotolerance in these breeds, as well as a possible effect of environmental variables on the prevalence and clinical characteristics associated with the infection. The epidemiological and economic implications of these findings are discussed here.https://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0001394289https://orcid.org/0000-0001-5839-8523https://scienti.minciencias.gov.co/gruplac/jsp/visualiza/visualizagr.jsp?nro=00000000000695jeiczon.jaimes@campusucc.edu.cohttps://scholar.google.es/citations?user=JfcTKEIAAAAJ&hl=es8 p.Universidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Medicina Veterinaría y Zootecnia, BucaramangaElsevier B.V. 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Antioquia, Colombia. http://opac udea.udea.edu.co/query.php?1282552AnimalTrypanosomiasisPrevalenciaRazas CriollasAnálisis clínicoAnimalTrypanosomiasisPrevalenceCreole breedsClinical analysisMolecular surveillance of Trypanosoma spp. reveals different clinical and epidemiological characteristics associated with the infection in three creole cattle breeds from ColombiaArtículos Científicoshttp://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionNINGUNAinfo:eu-repo/semantics/closedAccesshttp://purl.org/coar/access_right/c_14cbPublicationORIGINAL2022_molecular_surveillance_trypanosoma-FormatoLicenciaUso.pdf2022_molecular_surveillance_trypanosoma-FormatoLicenciaUso.pdfLicencia de Uso de Obraapplication/pdf238225https://repository.ucc.edu.co/bitstreams/6fc3978e-ed14-4677-8317-85f128b7a335/download08989198860dad9e696ea9c63bcd6cbaMD522022_molecular_surveillance_trypanosoma.pdf2022_molecular_surveillance_trypanosoma.pdfArtículo científicoapplication/pdf2397092https://repository.ucc.edu.co/bitstreams/7d0f9af3-3070-4cb7-b123-56d920f7a90b/downloadd4b8481308852562d0d11a17c5bfeed2MD53LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repository.ucc.edu.co/bitstreams/fc08fe36-eb2e-4053-add4-06a8b859e743/download8a4605be74aa9ea9d79846c1fba20a33MD54THUMBNAIL2022_molecular_surveillance_trypanosoma-FormatoLicenciaUso.pdf.jpg2022_molecular_surveillance_trypanosoma-FormatoLicenciaUso.pdf.jpgGenerated Thumbnailimage/jpeg4892https://repository.ucc.edu.co/bitstreams/d7d3e57d-b21b-4802-b358-f031bdc269c2/download5014c6649810ec65a2b969443772b60bMD552022_molecular_surveillance_trypanosoma.pdf.jpg2022_molecular_surveillance_trypanosoma.pdf.jpgGenerated Thumbnailimage/jpeg5577https://repository.ucc.edu.co/bitstreams/ba00f58a-c32f-4d29-9f04-217de9d3c280/downloada4af5cbfa5f2544a4db62085bb054b3cMD56TEXT2022_molecular_surveillance_trypanosoma-FormatoLicenciaUso.pdf.txt2022_molecular_surveillance_trypanosoma-FormatoLicenciaUso.pdf.txtExtracted texttext/plain6166https://repository.ucc.edu.co/bitstreams/7dfe0766-b6c2-46c6-a895-988b30ac32a4/download2207673b671d1e78c4c1bd5e38068a69MD572022_molecular_surveillance_trypanosoma.pdf.txt2022_molecular_surveillance_trypanosoma.pdf.txtExtracted texttext/plain54088https://repository.ucc.edu.co/bitstreams/686c9c24-eadb-4035-bf87-3f51f96dc37b/download6ea47fbf9483a45027f908a15ce7ac91MD5820.500.12494/46387oai:repository.ucc.edu.co:20.500.12494/463872024-08-09 12:27:14.661restrictedhttps://repository.ucc.edu.coRepositorio Institucional Universidad Cooperativa de Colombiabdigital@metabiblioteca.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

Molecular surveillance of Trypanosoma spp. reveals different clinical and epidemiological characteristics associated with the infection in three creole cattle breeds from Colombia

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